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koopa_troopa
the fun guy


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Producing a commercially viable method for inoc. truffle saplings! UPDATED with pre-TEK
#9351812 - 12/02/08 04:59 PM (4 years, 6 months ago) |
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I have been cultivating for several years and have somewhat mastered the cultivation of Psilocybe cubensis, and have enjoyed great success lately with several edible strains. I am by no means a master of fungi cultivation, but am very confident in my skills as a cultivator. After stumbling upon this article http://www.shroomery.org/forums/showflat.php/Number/8337353#8337353 which asserts that the region of North Carolina in which i reside is perfect for North American truffle production. In the article Ms. Rice, the owner of what will presumably become one the largest truffle operations in North America, states that the truffles will be harvested once every five years,which she expects to bring in up to $15 million during harvest. If she is successful, I predict this will trigger an emense regional influx of farmers wishing to establish similar operations. Due to the difficulty of cultivation, substantial time scale, and emense cost of successfully producing a viable truffle orchard, i believe it would be much more practical to focus on providing the saplings of Pubecent Oak trees whose roots have been innocullated with the spores of the White Truffle (Tuber magnatum) or the Black Périgord Truffle (Tuber melanosporum). I am posting this in hopes that some members who are more talanted and experienced than myself (which is probably a majority of this community) in fungi cultivation, can help me find the most effective method of innoculating the roots of these saplings. I have came across several methods, some of which are even patented. Most consist of making a bath of spore solution and submerging the roots in the bath, which sounds ALOT simpler than it actually is. I have found this publication very helpfull http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1449033 but would still like help in producing a simple and consistently effective method suitable for medium to large scale production operations. Ofcourse, this is a long term goal. For now, simply producing a handful of viable specimens would be paramount. I realize there was a thread on truffle production on these boards a couple years ago, but it died out awhile back and it focused on the complete cycle of truffle production, where this is only on the production of inoculated saplings. I know there are many brilliant and gifted cultivators on this forum and i could use as much help as i can get. I have already found several seriously interested investors and if anyone could help with bringing this dream to fruition, and i would gladly recipricate if this lead to a commercially viable method! I have purchased some truffle spore syringes and am sprouting several European Oak varieties from seeds. I am ready to get my hands dirty!
Edited by koopa_troopa (04/30/09 03:24 AM)
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denger
Mycelium keeper



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Re: Help producing a commercially viable method of innoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9352561 - 12/02/08 07:29 PM (4 years, 6 months ago) |
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This is an excellent endeavour, and good thinking on your part. My applause goes to you. While, I personally do not have any hands-on experience with truffles in particular, I would like to say that it is paramount to check that you actually have succeeded with your inoculations. One way to do this is by taking a piece of the root and checking under the microscope for mycelium interaction with the root tissue. Another note is that it might (or might not) be more beneficial to first grow diploid mycelium and use that to inoculate roots, similar to what is done with morels.
-------------------- Dennis, in Love with Fungi
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Breeding mushroom strains
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Forest Garden
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Re: Help producing a commercially viable method of innoculating seedlings with truffle spores!! [Re: denger]
#9357202 - 12/03/08 01:02 PM (4 years, 6 months ago) |
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i saw a website a few years ago that sold truffle inoculated hazelnut seedlings. you could just buy them and plant them in your yard. of course way too pricey if you wanted to plant multiple acres
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denger
Mycelium keeper



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Re: Help producing a commercially viable method of innoculating seedlings with truffle spores!! [Re: Forest Garden]
#9357787 - 12/03/08 02:42 PM (4 years, 6 months ago) |
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Quote:
Forest Garden said: i saw a website a few years ago that sold truffle inoculated hazelnut seedlings. you could just buy them and plant them in your yard. of course way too pricey if you wanted to plant multiple acres
I dont think thats what he's after. He want to make and sell those seedlings.
-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of innoculating seedlings with truffle spores!! [Re: denger]
#9368020 - 12/04/08 09:30 PM (4 years, 6 months ago) |
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hey, just got word back from the North Carolina Farm Prosperity Project.. they are currently offering free workshops and government funding in western nc! this is my next step, i am cultivating the pubescent oak and filbert seedlings as we speak!
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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shroomophile
ShroomitusFidelis



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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9373947 - 12/05/08 06:45 PM (4 years, 6 months ago) |
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These are just speculation on my part.I think truffles occur in old growth ares because it is a succession of organisms that it needs to fruit.When i can find a likely place to inoculate i would like to have the soil(even 1 to 5 pounds) from the area where a sclerotia is found.I would then pasteurize a load of similar substrate and then inoculate with the Master.I would then mulch my area with that.Just a thought.
-------------------- Once the mighty oak,was a nut who held his ground.
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spazn420
zombie mycologist



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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: shroomophile]
#9374220 - 12/05/08 07:16 PM (4 years, 6 months ago) |
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here is a site where they sell inoculated trees. Regards
http://www.truffletree.com/order.html
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: spazn420]
#9436555 - 12/15/08 02:47 PM (4 years, 6 months ago) |
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While laying in bed last night an idea struck me.. what if, somehow i was able to create a hydroponic system which consisted of not only nutrients for the saplings roots but also the truffle spores, logically this seems to be a very efficient and prosperous method of root inoculation. It sounded to great to be true but this morning, thanks to the omniscient Google i found this link http://www.springerlink.com/content/xr35880715887665/. Although the method pertains to the cultivation of the mushroom Cantharellus cibarius, the species is still a mycorrhizal fungi (the myc. must colonize the roots of a tree before fruit bodies can form). Does anyone know how one would keep such a hydroponic unit steril or if that is even necessary? i will probably have to purchase the journal unless someone just happens to subscribe to the Mycorrhiza journal lol, maybe RogerRabit?? i would love to know your thoughts if you stumble upon this.
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9436662 - 12/15/08 03:04 PM (4 years, 6 months ago) |
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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jjb007

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9438377 - 12/15/08 07:14 PM (4 years, 6 months ago) |
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Quote:
koopa_troopa said: While laying in bed last night an idea struck me.. what if, somehow i was able to create a hydroponic system which consisted of not only nutrients for the saplings roots but also the truffle spores, logically this seems to be a very efficient and prosperous method of root inoculation. It sounded to great to be true but this morning, thanks to the omniscient Google i found this link http://www.springerlink.com/content/xr35880715887665/. Although the method pertains to the cultivation of the mushroom Cantharellus cibarius, the species is still a mycorrhizal fungi (the myc. must colonize the roots of a tree before fruit bodies can form). Does anyone know how one would keep such a hydroponic unit steril or if that is even necessary? i will probably have to purchase the journal unless someone just happens to subscribe to the Mycorrhiza journal lol, maybe RogerRabit?? i would love to know your thoughts if you stumble upon this.
I know nothing about hydroponics or truffles, but I do know that truffles take about 5 years to develop. And it seems that running a hydroponics system for 5 years straight would suck up a lot of energy and may end up costing a fortune. But then again, I know nothing about hydroponics but it may be something to consider.
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denger
Mycelium keeper



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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: jjb007]
#9439319 - 12/15/08 09:33 PM (4 years, 6 months ago) |
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I think he means to use hydroponics to infect plants with truffles. Afterwards they will get transplanted into normal soil.
-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: jjb007]
#9443356 - 12/16/08 02:16 PM (4 years, 6 months ago) |
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jjb did you even read the thread or just my last post?? bc obviously you have no idea what the topic even is and if you know nothing of either topics and havent even bothered to have read the previous posts or the links i posted, why even post? you are wasting your time and mine.. i figured atleast somebody on these boards would be familiar with some sort of mycorrhizal fungi..
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
#9443418 - 12/16/08 02:25 PM (4 years, 6 months ago) |
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hey deng.. appreciate the interest you have shown in this thread even tho it appears to be going nowhere  lol atleast someone actually read my posts i believe thats the second time you corrected a poster.. btw i love the magnetic stirrer, really clean professional look to it. Where did you get the "double gang electric box" not even sure what the hell that is haha. I have two i have already made but i really want one with speed control.. will use your tek next
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9443802 - 12/16/08 03:32 PM (4 years, 6 months ago) |
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i have not decided yet on my method of root inoculation but i believe i have narrowed it down to two choices
1) employing a nutrient film technique (hydroponics), which would allow production the fungus on the roots of intact plants whose roots are bathed in a soil-less, nutrient solution.
Drawbacks: most methods of this require production in a greenhouse, which subjects inoculum is subject to contamination.
Possible Solution: Building a compact autoclavable hydroponic culture system for the production of axenic ectomycorrhizas and saplings. I found this method here http://www3.interscience.wiley.com/journal/119573041/abstract?CRETRY=1&SRETRY=0 --It describes a completely inclosed and autoclavical hydroponics system where "liquid is circulated by means of a low pressure air supply and sterility is maintained by glass fibre air-filters. Provision is made for sampling of the nutrient solution for analysis and sterility tests."
2) producing axenic (free from contamination) ectomycorrhizal fungi in association with plant root organ cultures on agar. The resulting root organ culture may be inoculated into whole plants.
Drawbacks: This technique would present substantial barriers to commercial scale-up on a cost effective basis. From what i have read, dual-member cultures are "relatively slow to develop and their long generation times (3-8 weeks from inoculation to harvest) limit the use of capital intensive, large scale fermentation equipment for production."
Solution: employing "porous substrates, as opposed to liquid or agar substrates to improve fungal growth and sporulation and to allow for economic inoculum production. This method entails using a medium comprising porous substrates such as vermiculite wetted with nutrient solution for the development of axenic fungi." This website details this technique http://www.freepatentsonline.com/EP0209627.html although i would probably have to alter the method if this patent becomes accepted until then.. let the games begin! 
If anyone is familiar with ANY of these techniques please weight in on the subject. I would be ecstatic if RogerRabbit droped by to throw his oppinion into the ring hint hint
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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jjb007

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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9444361 - 12/16/08 05:19 PM (4 years, 6 months ago) |
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Quote:
koopa_troopa said: jjb did you even read the thread or just my last post?? bc obviously you have no idea what the topic even is and if you know nothing of either topics and havent even bothered to have read the previous posts or the links i posted, why even post? you are wasting your time and mine.. i figured atleast somebody on these boards would be familiar with some sort of mycorrhizal fungi..
Yes I did... Although, as I heavily stressed, I do not know much of anything about the topic... I was just trying to provide some information that may be useful. Apparently it was not at all useful, but oh well, at least I tried.
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denger
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9444991 - 12/16/08 06:57 PM (4 years, 5 months ago) |
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Quote:
koopa_troopa said: Where did you get the "double gang electric box" not even sure what the hell that is haha. I have two i have already made but i really want one with speed control.. will use your tek next
Home depot has them, any large hardware store or electrical supply store would have them.
-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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denger
Mycelium keeper



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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9445026 - 12/16/08 07:03 PM (4 years, 5 months ago) |
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Quote:
koopa_troopa said: 2) producing axenic (free from contamination) ectomycorrhizal fungi in association with plant root organ cultures on agar. The resulting root organ culture may be inoculated into whole plants.
I am actually quite familiar with the plant tissue culture in general and this particular approach. Commercial scale is not a problem at all. Getting it all to work to begin with is a HUGE problem. You will need tons of equipment, chemicals (for the plant tissue media) and quite a bit of know-how. I would recommend sticking with hydroponics, or using the soil methods used by morel growers. I can dig up a link to their method if you'r interested. Basic idea is to grow mycelium aseptically (agar or LC) then make spawn on appropriate media, then mix spawn with sterilized soil and grow your seedlings in this soil for a month or two. Afterwards take a root tissue sample and check under the microscope for the presence of fungal-plant interactions.
-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
#9446211 - 12/16/08 10:37 PM (4 years, 5 months ago) |
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def interested in the link! how much start up would you estimate it would take to begin a small opperation with plant tissue cultures? this seems like it would be more efficient method in the long run. the problem with the hydroponics is that i would have to find a system that can withstand autoclaving or build one, then must be inclosed in a glass inclosure similar to a giant grow box and autoclaved to insure an acceptable rate of inoculation and contam prevention.. but this method seems to have a very respectable success rate but seems that this might be difficult to do on a large scale... although i have found many examples of mycologists that are creating and patenting these systems as we speak. As for the soil method, is the substrate pasteurized? grown inside under lights or in a greenhouse? i have read up on some of this and it seems they are pretty contam prone, when this method in conjunction with greenhouses is used. Thanks for the info!
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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denger
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9446661 - 12/16/08 11:41 PM (4 years, 5 months ago) |
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I think you are paying too much respect to sterile conditions. That would be important if you'r using spores, because contaminants easily take over media and suppress spore germination. If, instead you would grow your mycelium as you would any other mushroom, and use this grown mycelium to infect plants, the infection stage can be done in unsterile conditions. After all, the seedlings will find their way into the unsterile soil.
Check this out: http://www.morel-farms.com/discovery.html
Definitely read their patent too. This is very close to what you want to do.
As much fun plant tissue culture can be, I would not recommend going in that direction unless you just want to have a very expensive hobby.
-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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Speeker
www.speeker.tk

Registered: 02/11/04
Posts: 613
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
#9448104 - 12/17/08 03:28 AM (4 years, 5 months ago) |
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don't know how "commercially viable" method this is, but here is a video where they are inoculating seedlings by hand with mycelium on agar.
mms://winmedia.cctv.com.cn/natureandscience/2006/11/natureandscience_128_20061128_3.wmv
Quote:
The Family of Fungi(Part 4): The Temptation of Truffles Source: CCTV.com 11-28-2006 16:34
For hundreds of years, truffles have been sought as a rare delicacy at a high price. They are often regarded as the “caviar” of fungi. They only grow underground in forests, and can only be tracked down by certain animals. In Italy and France, tamed pigs are used to locate truffles. The dark blocks which countryside people ate in the past are now luxurious food for international VIPs.
Even today, the methods used in finding truffles in forests of oak, white poplar, hazel or beech are unchanged after hundreds of years. Truffle hunters must have patience and know how to control their pigs, as pigs are clever and omnivorous. Pig trainers must also make sure to check them in time or they will eat all the truffles. The quality and size of truffles vary with the species and place of origin, and will be evaluated after picking.
Autumn is the traditional season for hunting for truffles. Soon after sunrise, many quiet households begin to get busy. Dogs are ready to begin the day’s work. This dog is well trained for seeking truffles, and is an indispensable assistant for his master, a professional truffle picker in Burgundy. The first thing they do every morning is head into the forests. There is no need to persuade the dog, because it shares the happiness of finding a truffle with its master.
In Burgundy and other truffle production bases in the Mediterranean area, the practice of seeking truffles with pigs is decreasing and more and more dogs are being employed, as they are faster and have a keener sense of smell. They are trained in special schools or by their masters. Clever masters will give them a certain degree of freedom so that they can accomplish the task themselves. They are usually as enthusiastic as their masters.
Before washing, truffles are not particularly appealing to the eye. Experts can determine the quality of truffles from their scent. After picking a truffle, the dog will be rewarded with a piece of cheese. Before leaving the site of picking, the picker will cover the pit with soil and leaves to protect the mycelium of the truffle so that more truffles will grow. For those that are lucky, a few hours of truffle hunting in the morning may result in a few great finds. When a Burgundy or Perigord truffle is cut into halves, it shows a special texture. Its special flavor can be used to cook some of the most delicious dishes in the world.
This is the Vienna Institute of Botany. Truffles have been studied here for a long time as a special topic. Botanist Alexander Eben believes that truffles will be cultivated artificially on a large scale in the future. Like boletus, truffles are symbiotic with the soft roots of some trees. The experiment they are attempting is to graft germinant truffle substances onto the roots of young trees. Several hundred young trees have been grafted and have been growing in green houses for two years. The researchers check their growth frequently after washing off the soil. They have found that the roots are growing longer and thicker, and they are on the road to success.
In three or four years, the first batch of farmed truffles may be grown in the Austrian truffle garden. What an exciting prospect! These so called white truffles are light in color and inferior in quality. But they are still highly valued. They grow in Austrian forests and can be found by experienced pickers, usually after a storm has washed off the topsoil on hill sides and exposed the bulb like truffles. Volt Korafak begins looking along the two sides of the valley after a rainfall. His patience is often paid off. Colorful and attractive fungi grow and propagate everywhere in the forest. An ancient Siberian legend said they were driven by the magic power of the spirits of fungi.
http://www.cctv.com/program/natureandscience/20061128/104104.shtml
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denger
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: Speeker]
#9564249 - 01/07/09 02:31 PM (4 years, 5 months ago) |
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-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
|
koopa_troopa
the fun guy


Registered: 01/12/08
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
#9570950 - 01/08/09 03:31 PM (4 years, 5 months ago) |
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Hey Denger.. good to hear from you bro. I am actually doing research on the subjuct right now in my campus library.. thanks for the link man bout to check it out now.. will get back soon.
I am beginning the first round of experiments in two weeks, it would be great if anyone had any advice or knew of any places where i could possibly gain access to a larger autoclave.. or perhaps a good source on used autoclaves, even building instructions.. dont worry i wont blow myself up, my partner is a mechanical engineer
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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denger
Mycelium keeper



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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#9571081 - 01/08/09 03:50 PM (4 years, 5 months ago) |
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Building autoclaves is not the best way to spend your time/money. You will end up spending more $$ on materials and parts then on buying a brand new one. How big do you need it? AA pressure cookers go all the way to 41qt size, thats twice the size of a standard issue medical autoclave. Check ebay if you need bigger. Your best bet for bigger ones is a "retort" made for canning industry. Again, ebay, or canning industrial supply place.
-------------------- Dennis, in Love with Fungi
My improved magnetic stirrer
Breeding mushroom strains
Potato-Honey-Yeast-Agar Tek
Looking for Chantarelle and Armillaria cultures, have a huge collection of other edibles to trade.
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Amanita Sporosa
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: denger]
#9748735 - 02/06/09 11:55 AM (4 years, 4 months ago) |
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I am generally interested in this subject. I would like to learn how to grow truffles hydroponically. Let me know how your experiments turn out.
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koopa_troopa
the fun guy


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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: Amanita Sporosa]
#9750268 - 02/06/09 05:09 PM (4 years, 4 months ago) |
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Hey everybody, sorry for leaving this thread hanging for awhile for those that where interested. I am starting to actually make some progress now. Deng, thanks alot man for the interest and the wisdom you have provided.
Amanita, i suggest you read all the links ive provided on here and if you have access to a university, I strongly suggest that you use their computers to read as many scientific journal articles on any of the tuber genus and ecto and endomycorrhizal studies, research and patents you can find.
It is a subject very clouded in secrecy and a general lack of solid knowledge, which I have come to believe that to some extent is very purposefull.
The only advice I can give you at the moment is that I have came to learn that most of the methods I stated above are more suited towards acadamia and research and are impractical for field use and large scale production. I will update this thread after my first round of experiments are done, hopefully within the following month.
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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koopa_troopa
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#10260128 - 04/30/09 03:10 AM (4 years, 1 month ago) |
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So im about to begin work on site preparation for beginning my truffle orchard. Ive received close to 200 acres of farm and field land, along with all the major equipment I will need to cultivate it. Cant believe everything is falling into place so easily for once in my life
Ive been keeping correspondence with both Tom Michaels (of Tennessee Truffles fame) and Gerard Chevalier (THE truffle expert from France) who have both provided invaluable help. I will be starting a new thread soon which will detail the growth and progress of my opperation, but for now I will lay out some general info to give everybody something to chew on and to also help me get this shit out of my brain lol been obsessively researching all things Truffle related for some time now.. Lord knows the girlfriend is Fn tired of even hearing the word spoken God bless er' lol.
Next week, after my soul raping final exams are over with, I will begin construction of two large greenhouses in which Oak and Filbert saplings will be sprouted and inoculated in. After finishing construction, I will begin land preperation which includes tilling the land, and spreading approximately 1 ton of lime per acre to achieve the proper PH. Thankfully one of my friends owns a Grading company and has very cheap access to bulk amounts of lime ($50 per 1/2ton!) and also a spreader that holds 1/2 ton per load. Next, I will begin fertilizing the land, which ingredients will remain somewhat secret but you can get a pretty good idea by studying patents and research papers on the subject at university libraries .
Site selection for truffle orchard development is also very important.. ideally you should look for land that is similar to any that would be ideal for farming.. large open areas that would be easy to irrigate and most importantly, land that has not had prior tree growth recently. This helps limit the amount of domestic mycorhizal competition for the truffle to overcome. Tree selection is also very important. In the primary testing and selection stage, one should test several species prevalent in truffle preduction in France with native candidates (usually those of the Oak variety). Excellent info on this subject can be found in Tom Micheals research thesis on Invitro Truffle Growth. Once ideal host candidates are determined through random statistical analysis on growth vigor and mycorhizal abundance you should be feeling like a certified bad ass.
Next I will describe a simple method for developing the mycorhizal symbiosis between the tree and the truffle fungis. First, oak seeds must be surface sterilized with a .1% solution of silver nitrate for 20 minutes and then rinsed well with sterile water to remove all remaining traces of the substance. Next one must acquire fresh or frozen truffles allowing them to thaw if frozen then place in a jar or similar container filled with water allowing the truffle to degredate slightly. After degredation, dissolve 1 kg of dextrose or similar simple sugar per 20 liters of truffle suspension, creating what is called a truffle slurry. Some truffliers (growers of the truffle, duh ) like to add snail enzymes to the slurry claiming beneficial effects on mycorhization rates.
Next, you must prepare the potting soil. Each trufflier has their own secret potting mix, but a simple example is 20% loess soil, 30% organic material, 50% vermiculite, dolemite to raise PH and slow release Osmocoat fertilizer. The soil mix should then be pastuerized to remove detrimental microorganisms. Mix the truffle slurry into the potting soil mix (again, many ideal soil preperations can be found in many truffle patents and research papers) once the tree outgrows its pot after a few months, remove it and gently wash the roots with water to view its inoculation process, noting growth rates and statistical differences between other saplings. Next, dip the roots in a second mix of truffle slurry before adding it to a larger pot. This should all be done in an enclosed greenhouse or a lab/controlled grow room to minimize contam rates and controlled environmental factors to reduce sapling deaths and increase inoculation rates. After saplings have reached a year or two in age, they may be transferred to the previously prepared land to hopefully live long and happy truffle bearing years, preferably while you sip lemonade and laugh at wall street douche bags.
After 4-10 years you will hopefully begin to notice the ground to begin to knot around the trees, followed by burn circles causing the death of the grass around the trees. You should begin to notice a sweet pungent odor in the air, next tell any low life, favor asking, money borrowing brother in law to go frack a swine flu infested Panama hooker... while you and Chittlins (your prize winning truffle finding pig) cruise town in your drop-top 67 Vette!
Appologies for all the spelling/gramatical errors.. 3am and taking a study break from finals, figured I would share a cliff notes version of my future truffle orchard. Hope everyone enjoys the read, and keep a look out for the picture tutorial ill be making in the next couple weeks! Wish me luck, ill sure as hell need it!
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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Mycelio
Stranger


Registered: 06/24/08
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#10260845 - 04/30/09 07:57 AM (4 years, 1 month ago) |
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Hey Koopa_troopa,
very interesting, thanks for sharing! It seems you got pretty far in a short time. Now I'm looking forward to reading more and seeing some images.
Carsten
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koopa_troopa
the fun guy


Registered: 01/12/08
Posts: 374
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: Mycelio]
#10264665 - 04/30/09 08:53 PM (4 years, 1 month ago) |
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Thanks alot Mycello, Ive been literally obsessed with the topic since the initial post.
I hope to eventually start my own company supplying inoculated saplings to other truffle orchard startups once a get a few years of hands on practice inoc'n several thousand trees .. the NC Agricultural Foundation estimates that within the next 10 yrs, the amount of truffle orchards in this state alone will increase some 5000%. And with average orders for saplings per orchard between 600 and 5000, and the avg price per sapling being between $30-$80.. there should be some pretty nice potential for profit.
The NC Agricultural Foundation also estimates that a properly managed truffle orchard could net around $30,000 per acre once they begin bearing truffles, and possibly for 20-40 years!!! So with 250 acres of prime truffle growing land at my disposal, and between two of my best friends becoming partners with me, who just happen to own all the possible farming and grading equipment I could ever possibly need.. Im giddier than a Mormon virgin in Vegas
Its incredible how after years of life taking a shit on you, how suddenly one random day you can turn around and make life your bitch for a change haha.. But seriously I couldnt be happier with how everything is just falling into place so seamlessly.. ill be damned if I let this fish off the hook! All thats standing in my way now are the next two weeks of hellaciously mind rapping final exams..lol wish me luck
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Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars
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SOUTHERN
NAIL DRIVER



Registered: 03/29/09
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Re: Help producing a commercially viable method of inoculating seedlings with truffle spores!! [Re: koopa_troopa]
#10265463 - 04/30/09 10:42 PM (4 years, 1 month ago) |
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That sounds great koopa .. I need to order me some sapplings. I've got some land i'd love to put some of those trees on.
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