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Offlinethatsstupid
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Mixing 2 strains in 1 jar?
    #8797647 - 08/19/08 06:15 PM (15 years, 7 months ago)

can you mix 2 strains in 1 jar?


i have a half pint BRF jar in the pot sterilizing right now. When it cools down can I inject 2 strains in the jar?

in 2 inoculation holes?


Cambodian and Mexi-Albino are the strains.

i just wanted to get a for sure answer before I did it. just as an experiment.



NOTE: I also have LCs growing of both strains.


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlinedjblackout
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8797653 - 08/19/08 06:16 PM (15 years, 7 months ago)

you can do anything you want,  you could inject feces follicles into the jar even....


will it work?  idk.


--------------------
I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You

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Offlinekillerthc
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8797674 - 08/19/08 06:21 PM (15 years, 7 months ago)

Quote:

thatsstupid said:
can you mix 2 strains in 1 jar?


i have a half pint BRF jar in the pot sterilizing right now. When it cools down can I inject 2 strains in the jar?

in 2 inoculation holes?


Cambodian and Mexi-Albino are the strains.

i just wanted to get a for sure answer before I did it. just as an experiment.



NOTE: I also have LCs growing of both strains.




either 1 will take over the other or they will live just fine.

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Offlinefunguy_84
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Re: Mixing 2 strains in 1 jar? [Re: killerthc]
    #8797692 - 08/19/08 06:23 PM (15 years, 7 months ago)

waste of time and SS.


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OfflinePowerOfTheCoir
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Re: Mixing 2 strains in 1 jar? [Re: funguy_84]
    #8797762 - 08/19/08 06:42 PM (15 years, 7 months ago)

I heard that they'll fight each other and sometimes even break the jar.


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OfflineFictionalPersona
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Re: Mixing 2 strains in 1 jar? [Re: PowerOfTheCoir]
    #8797801 - 08/19/08 06:52 PM (15 years, 7 months ago)

Quote:

PowerOfTheCoir said:
I heard that they'll fight each other and sometimes even break the jar.



Lol irl
would be kinda cool to have different looking shrooms on each side of the cake.

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: FictionalPersona]
    #8797813 - 08/19/08 06:55 PM (15 years, 7 months ago)

i know. that's why i wanna do it. =P


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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OfflineRogerRabbitM
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8798138 - 08/19/08 08:12 PM (15 years, 7 months ago)

Do it.  You'll be injecting hundreds of 'strains' with each injection anyway, regardless of the name on the syringe.

Remember, when hyphae from two compatible spores meet up and exchange genetic information, a strain is born.  When you inject 1 ml of solution from a spore syringe, you perhaps inject 50,000 or more spores.  Imagine all the strains created.  Since cubensis is a species, spores from many strains are going to be compatible. 

Through a process called anastomosis, the strains generally merge into a common whole as clamp connections form between mycelium from various strains.  Non-compatible strains will often grow and fruit separately, giving a flush of distinctly different mushrooms.
RR


--------------------
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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: RogerRabbit]
    #8798150 - 08/19/08 08:14 PM (15 years, 7 months ago)

So what your saying is it will work?


-.-

i injected 2 holes of Mexi Albino

half a CC each.

is that good?


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlinespazn420
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8798166 - 08/19/08 08:17 PM (15 years, 7 months ago)

Damn, I learn something new everyday! I am going to have to try it now. Thanks RR for your plethora of information!


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OfflineMetatron21
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Re: Mixing 2 strains in 1 jar? [Re: RogerRabbit]
    #8798220 - 08/19/08 08:30 PM (15 years, 7 months ago)

Quote:

RogerRabbit said:
Through a process called anastomosis, the strains generally merge into a common whole as clamp connections form between mycelium from various strains.  Non-compatible strains will often grow and fruit separately, giving a flush of distinctly different mushrooms.
RR




Wow that is remarkable.  I'd love to see this, what is the probability of this happening if you injected two strains, as the original poster is probably doing, or already did.


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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: Metatron21]
    #8798751 - 08/19/08 10:16 PM (15 years, 7 months ago)

Ok so the half pint jar has 1 CC of each strain in it.


4 holes. half a CC on each hole.


on our jar.. we dont have the lid part.. the middle.  we have the scew on lid.

what we did was put a piece of foil on top of the jar and screwed it on.

then put another piece of foil on top.


when i inoculated i went to far and the pink part of the needle at the bottom went in the foil so i have 2 kinda big holes from inoculation in the foil lid thing....


i hope it's ok. i put another layer of foil on top of the jar.


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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OfflineRogerRabbitM
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8798770 - 08/19/08 10:20 PM (15 years, 7 months ago)

I'd only use one layer of foil.  The dry verm is your filter, and you'll need gas exchange or the future mycelium will stall.
RR


--------------------
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OfflineDesertWolf
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Re: Mixing 2 strains in 1 jar? [Re: PowerOfTheCoir]
    #8798778 - 08/19/08 10:22 PM (15 years, 7 months ago)

Quote:

PowerOfTheCoir said:
I heard that they'll fight each other and sometimes even break the jar.




You've seen that happen to? :tongue2:


--------------------
Those that govern democracy, Nothing but lies and hypocrisy, Created by those who pretend to heal you, Bearers of the cross who try to control and conceal you, And they will die before it reveals to you, What you haven't been programmed to see.

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: RogerRabbit]
    #8798779 - 08/19/08 10:22 PM (15 years, 7 months ago)

ok thanks RR. ;]


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlinedjblackout
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8799387 - 08/20/08 02:03 AM (15 years, 7 months ago)

that makes me wonder what would happen if you..

crumble 2 cakes of 2 different strains and cased them?


--------------------
I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You

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OfflineFictionalPersona
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Re: Mixing 2 strains in 1 jar? [Re: djblackout]
    #8807012 - 08/21/08 05:54 PM (15 years, 7 months ago)

It would probably work even better since the myc is already established the its own substrate they would probably interfere less growing through the casing.

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: FictionalPersona]
    #8807065 - 08/21/08 06:05 PM (15 years, 7 months ago)

this is great i cant wait ill put up pics when we fruit so everyone can see lol
:tongue:
-Michael-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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OfflineG1assjaw
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Re: Mixing 2 strains in 1 jar? [Re: FictionalPersona]
    #8807091 - 08/21/08 06:09 PM (15 years, 7 months ago)

i have a jar or EQ and texas going right now and the jar is doing good. I did hear tho that one strain will become dominant so you will end up with just one stain in the end. however idk. i guess you just have to do it and see.

-g1assjaw

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Offlinedjblackout
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Re: Mixing 2 strains in 1 jar? [Re: G1assjaw]
    #8807397 - 08/21/08 07:00 PM (15 years, 7 months ago)

but even with crumble and casing with each other?


--------------------
I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: djblackout]
    #8816232 - 08/23/08 03:08 PM (15 years, 7 months ago)

Ok so our jar has 2 spots of mycelium growing... oposite sides.

i'm guessing the cambodian got a headstart..... since it's suppose to be a fast colonizer.

no signs of growth on the other 2 sides where i "think" mexi-albino was inoculated.


pics will be up soon.



EDIT: I took pics with my phone and put them on my memory card but I can't find the adapter for my memory card to put in my computer. Once I find it i'll post pics. The pictures include: Our incubator, The multi-strain jar, Mexi-Albino LC, & Cambodian LC.


GaRy-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



Edited by thatsstupid (08/23/08 03:42 PM)

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8818137 - 08/23/08 10:45 PM (15 years, 7 months ago)

Here are some pics I took with my phone.

The temp in the incubator is 83F.

Mycelium growth showing after 4 days in Mexi-Albino..

nothing really in Cambodian LC . not that I can notice.
























GaRy-

Edited by thatsstupid (08/25/08 04:12 AM)

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8818688 - 08/24/08 02:00 AM (15 years, 7 months ago)

can any one tell me if that's mycellum or contaminant plz


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlinedemonicaeroponic
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8818710 - 08/24/08 02:08 AM (15 years, 7 months ago)

I don't see any contams.


--------------------
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OfflineScavengerType
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8818747 - 08/24/08 02:27 AM (15 years, 7 months ago)

it looks like a contam but it could just be the blurry pics. Do you have a real camera? myc is one thing where blurry pics won't tell the truth rightly.

Edit: haha see 2 contradictory guesses right off the bat.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

Edited by ScavengerType (08/24/08 02:28 AM)

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OfflinePowerOfTheCoir
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Re: Mixing 2 strains in 1 jar? [Re: ScavengerType]
    #8819163 - 08/24/08 08:07 AM (15 years, 7 months ago)

My vote is for Myc.


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Offlinewillowmp
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Re: Mixing 2 strains in 1 jar? [Re: PowerOfTheCoir]
    #8819210 - 08/24/08 08:22 AM (15 years, 7 months ago)

don't worry bout contams if its your first time its probably mycelium. i went a good week thinking my jars were infected but they weren't.


--------------------
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OfflineRogerRabbitM
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Re: Mixing 2 strains in 1 jar? [Re: willowmp]
    #8819225 - 08/24/08 08:29 AM (15 years, 7 months ago)

You better worry about contamination.  That's too small a container and CO2 will build up in it really fast, choking off the mycelium.  In addition, 83F is too hot, since mycelium slows down at any temp of 83F and above, and it's always 2F to 5F warmer inside the jars.  Don't heat above 80F if you feel you need to heat at all, which you don't.

You can't hold a camera in one hand and a jar in the other and expect a good picture. There's too much movement in your arms, and having both arms moving really blurs the picture. Put the jar down and rest the camera on a book or something.

There's no way to tell from a blurry picture if it's mold or not.
RR


--------------------
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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: RogerRabbit]
    #8822191 - 08/24/08 08:55 PM (15 years, 7 months ago)

well today gary went home and informed me that the jar myceleum grew now its double sized both strains are growing i think thats what he said also our LC's have sum activity im pretty sure he will upload sum pics so u all can see :grin::mushroom2:

-Michael-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8823315 - 08/25/08 03:50 AM (15 years, 7 months ago)

here are pics of 2 sides of the jar. 2 inoculation holes right across from each other.
the other 2 sides have smaller spots of mycelium growing. i'm guessing that's Mexi-Albino.
Cambodian must be the bigger spots. Since it's a fast colonizer.


NOTE: I couldn't get a good picture of the LC's. I'll get some up later.

oh yeah it's been 6 days since inoculation and all that. for LC's and the jar.

we started on the 19th it's the 25th.







GaRy-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



Edited by thatsstupid (08/25/08 04:09 AM)

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OfflineScavengerType
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8824418 - 08/25/08 11:41 AM (15 years, 7 months ago)

yep links like healthy myc, thanks for the better pics.

BTW when it gets that the 2 colonizing points meet you may find that rather than meld they fight each other and ruin your cakes assuming they are different strains like the thread title says.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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OfflineRogerRabbitM
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Re: Mixing 2 strains in 1 jar? [Re: ScavengerType]
    #8825623 - 08/25/08 03:46 PM (15 years, 7 months ago)

Quote:

when it gets that the 2 colonizing points meet you may find that rather than meld they fight each other and ruin your cakes




There's not a single shred of evidence to support that.

'Strain' is similar to 'race' or 'breed' in animals.  You're saying if a german shepard mates with a rotweiller the genes are going to fight and ruin the litter?  How about if a man from florida mates a woman from texas?  The kid is going to fight with his own genes and abort? I've been growing mushrooms for over 35 years and am still waiting to see my first mycelium fight. Don't repeat nonsense just because some other noob repeated it before.  These silly myths are hard to kill.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlinespazn420
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Re: Mixing 2 strains in 1 jar? [Re: RogerRabbit]
    #8825675 - 08/25/08 03:56 PM (15 years, 7 months ago)

lol! Well put RR! Love it!  :rockon:


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Re: Mixing 2 strains in 1 jar? [Re: spazn420]
    #8825766 - 08/25/08 04:15 PM (15 years, 7 months ago)

no need to bite my head off about it, it's not like I was the first person to say it here.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: ScavengerType]
    #8825848 - 08/25/08 04:33 PM (15 years, 7 months ago)

beautiful i cant wait to eat them shrooms how long dose fruiting take after colonization

-Michael-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlinespazn420
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8825873 - 08/25/08 04:38 PM (15 years, 7 months ago)

wait 5 days after you see mycelium everywhere then dunk and roll!


--------------------

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: spazn420]
    #8825915 - 08/25/08 04:48 PM (15 years, 7 months ago)

ok but after all that when will see pins or something i cant wait im very excited

-Michael-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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OfflineScavengerType
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8825926 - 08/25/08 04:49 PM (15 years, 7 months ago)

It'll take a little while (few days) after you birth it and dunk and roll.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Offlinethatsstupid
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Re: Mixing 2 strains in 1 jar? [Re: ScavengerType]
    #8826269 - 08/25/08 05:59 PM (15 years, 7 months ago)

OK COOLLLL


today we went out in the field again and checked out our spot and found 7 pans growing in 1 shit.

they're still small we left them to grow and we will check them out later tonight or tomorrow afternoon or morning.


GaRy-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8831992 - 08/26/08 06:36 PM (15 years, 7 months ago)

well we got contaminants this fucken sucks ill upload pics in a minuet


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Re: Mixing 2 strains in 1 jar? [Re: djblackout]
    #8832171 - 08/26/08 07:07 PM (15 years, 7 months ago)

my bro just now put three strains together into one jar(im not sure why he did this and i told him it wouldnt work).  He used amazonians b+ and puerto ricans that had been left over from the birdseed casings he made. So picture three fully colonized bird seed jars each 1/3 full, mixed together into one jar. Right now healthy mycelium, of some sort or another, has colonized the whole jar(break it up it recolonizes, break it up again it recolonizes again).  I have no idea what is going to happen at this point but this thread makes me wonder.


--------------------
"Ive calculated your chance of survival but
I dont think your going to like it"


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Re: Mixing 2 strains in 1 jar? [Re: zak007]
    #8833375 - 08/26/08 10:47 PM (15 years, 7 months ago)







IS there any way to save it like could i break off the black shit or sumthin lol :confused:


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlineabitavenger
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Re: Mixing 2 strains in 1 jar? [Re: thatsstupid]
    #8833404 - 08/26/08 10:55 PM (15 years, 7 months ago)

nope its trash. throw it out in the garden


--------------------
Karma.

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InvisibleAdoreChampignons
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Re: Mixing 2 strains in 1 jar? And Creating Hybrids. [Re: thatsstupid]
    #8833405 - 08/26/08 10:55 PM (15 years, 7 months ago)

Quote:

thatsstupid said:
can you mix 2 strains in 1 jar?

i have a half pint BRF jar in the pot sterilizing right now. When it cools down can I inject 2 strains in the jar?

Cambodian and Mexi-Albino are the strains.




I'm sure you can mix two strains in a jar.  We often forget that these mushrooms normally co-habit with a multitude of bacteria and other fungi.  If the strain of mushroom is from the same genus, then there's a good chance that they'll co-habit well or even exchange genetic information to create a hybrid.  However, from the procedure that you're describing, hydrides are not that likely to appear.

The reason that hybrids may not appear is due to the way hybrids are created.  Please keep in mind that when a mushroom spore germinates, it has only half the genetic material it needs.  Before it can produce a mushroom cap, it first needs to bump into another germinating spore that also has half the genetic material.  Once these two meet and clamp onto each other and mate, they combine their genetic material are now able to grow usually faster, and now produce mushrooms.  In your case however, if you're inoculating the spawn jar at separate inoculation sites, due to the distance between inoculation sites, it's more likely that the germinating spores will mate with germinating spores of the same strain before even encountering germinating spores of the differing strain.

If you really want to create a hybrid between two strains of mushrooms, that is also possible.  However, it's a little tedious.  You first have to dilute the spores and grow them on nutrient agar.  The spores have be so diluted so that when they are smeared across the agar surface, they grow as individual colonies of mycelium as oppose to a thick cottony mat of mycelium.  Once you can see that a single spore has produced a little pin point of fuzzy mycelium, it has to be quickly isolated and transferred to a clean plate of nutrient agar.  Keep in mind that this mushroom mycelium only has half the genetic material needed to produce a mushroom.  The next step is to repeat the process with the strain that you want to create a hybrid with. 

Once you have establish two contamination free cultures having half the genetic material needed, you are ready to mate them and create the hybrid.  The best way I've read on how to do this is to touch the inoculation loop to the mycelium of one culture and drag it on the agar.  In dragging the inoculation loop on the agar, keep in mind that your goal is to draw a line on one side of the petri dish.  Repeat this process and draw a line on the other side of the same petri dish with the other strain of mushroom you want to mate.  Now as those two strains grow, they will meet predictably meet somewhere in the center. When they are about to meet, you can take a lower power dissection microscope and watch the mushrooms have sex. 

You usually can tell that mating was successful by the way the mycelium interacts as they meet.  When they meet, do they fold together nicely like the way your fingers fold together when you are about to pray?  Or, does it look like two fluffy waves of mycelium are bunching up and creating a sharp line of distinction?  If the mycelium meets and folds together smoothly, it usually means that there has been a successful mating.  If a sharp line of distinction appears, that usually means a number of things.  It could mean that the mycelium collected had already mated.  It could mean that the strains are incompatible at mating.  It could also mean that what you collected wasn't mushroom mycelium at all, but a contaminating fungi that blew into your petri dish.

Once you believe a successful mating has occurred, transfer the mycelium to your growing medium and incubate as usual.  The mushrooms that are created from this procedure may show a blending of characteristics of both parent mushroom strains, or only the characteristic of one parent,or neither.  Even though a successful mating has occurred, some traits may be dominant, others may be recessive.  While there is a big differ enc between mushrooms and men, you could see the same thing happening in children.  Sometimes they look like both parents.  At other times, they only look like the mother's side of the family.  And still, at other times they look like neither side of the family.  Go figure.

I hope this helped.  Did this make sense?  Are you going to try and creat your own super dooper msuhroom strain now?  Tell me what you think?

Cheers,

Mycophilic One


--------------------
There is no such thing as a dumb question.  There are just curious people trying to learn something new. 

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Re: Mixing 2 strains in 1 jar? And Creating Hybrids. [Re: AdoreChampignons]
    #8833506 - 08/26/08 11:18 PM (15 years, 7 months ago)

think anything will grow in my yard :grin:

and thanx AC  for the info


:stoned::thumbup:

-Michael-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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Offlineveda_sticks
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Re: Mixing 2 strains in 1 jar? And Creating Hybrids. [Re: thatsstupid]
    #8834520 - 08/27/08 07:00 AM (15 years, 6 months ago)

Those are for the trash, once contaminated theres no point in saving.

Check your sterilisation procedures and inoculation.

My guess is they got contammed during inoculatin. did you use a glovebox?


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinedjblackout
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Re: Mixing 2 strains in 1 jar? And Creating Hybrids. [Re: veda_sticks]
    #8836106 - 08/27/08 01:47 PM (15 years, 6 months ago)

i wouldnt even want to put the BLACK DEATH MOLD in my garden...


--------------------
I am the fakest person on this site. I only pretend to grow and consume illegal mushrooms. I have no knowledge what so ever on any scheduled substance because I know and respect the governing law in the United States of America. All pictures and dialogue posted by me is entirley copyrighted from those who wish to knowingly ignore the laws. I only post these messages as a mere propaganda technique used to gain attention and admiration from others. Thank You

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Re: Mixing 2 strains in 1 jar? And Creating Hybrids. [Re: djblackout]
    #8842540 - 08/28/08 03:50 PM (15 years, 6 months ago)

I offically think we suck at this hobby.

this is our second attempt and we still have no shrooms.


we keep getting fukin contaminated. i'm thinking about giving up and stop waisting my money on spores, verm, BRF, etc.

we do have wild birdseed. i'm thinking about trying that with the last of our mexi albino spores. about 4CC's.


and i have an LC of mexi albino. perfectly fine. 200ML - a syringe of the LC.

lots of mycelium in it. :laugh:


GaRy-


--------------------
"It's not enough. I need more. Nothing seems to satisfy. I don't want it. I just need it. To feel, to breathe, to know I'm alive."-Tool-
"And who are you and how can I try? Here inside I like metal Don't you?"- Nine Inch Nails
GOD IS DEAD!! AND NOOO ONEE CARES! IF THERE IS A HELL! I'LL SEEEE YOUUU THERE!!



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OfflineHazeyRoms
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Re: Mixing 2 strains in 1 jar? And Creating Hybrids. [Re: thatsstupid]
    #8843358 - 08/28/08 06:41 PM (15 years, 6 months ago)

Don't give up, that is how you never get success because you quit. This takes time and skill. Don't be a lame and quit this hobby, As for grains, see if you can handle the PF-Tek first. Then go for a PF to coir. More beneficial then just fruiting a cake.


--------------------
-Sonn, Hazey here. And I'm bacc. Anyone in Wisconsin, PM me. Unity my nillas/niggas.

-"5 Nike duffle bags??? How much does all that weigh?? - Just 30 pounds Bruhh, relax my nilla, it don't stink like weed so we're all good."-(Penis Envy Moments)-

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OfflineScavengerType
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Re: Mixing 2 strains in 1 jar? [Re: djblackout]
    #8843609 - 08/28/08 07:34 PM (15 years, 6 months ago)

you should get rid of the incubator and try some jars again. Are you inoculating in a sterile glove box?

Also how are you sterilizing your jars?


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

Edited by ScavengerType (08/28/08 08:48 PM)

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