Quote:

---

zouden said:
Both those methods have high cell mortality. Lipofectamine is much better, but less versatile. Cell mortality usually isn't an issue because there's no shortage of cells for this kind of work.

A brief list of things I've genetically engineered:

-sugarcane with gene gun: we were making a transgenic crop that produces a more complex carbohydrate than just sucrose
-bacteria with electroporation or heat-shock: various projects
-algae with glass bead vortexing: making a strain that can produce hydrogen gas through photosynthesis
-mammalian cells with lipofectamine: testing DNA before introduction into mouse - I used GFP as a marker, among other things
-mouse line with embryonic microinjection: I haven't done this personally, I'm outsourcing this to a specialist company. Roughly $2000 per mouse line.

The main methods that I haven't done are Agrobacterium-mediated gene transfer into plants and fungi, and viral vectors.

---

Quote:

---

Arp said:
so why did these mushrooms develop this feature? from an evolutionary standpoint.

perhaps its just a byproduct from something else

---