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OfflineDr. uarewotueat
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Cytokinin Experiment.
    #7506886 - 10/10/07 10:17 PM (7 years, 2 months ago)

so, i obtained some "keiki paste" which is used in the propagation of orchids, it is used to produce keiki's (baby plantlets) on the flowering spikes of certain types of orchid.
keiki paste contains a hormone called cytokinin which stimulates axillary branching in plants. so obviously it should work on cacti in the same way, at least i am hoping it will...
see here for explanation of keiki/keiki paste.



this thread is where i got the inspiration to try out this experiment. i cant claim that it is my idea, but i havent seen anyone post about this topic at all and it is something that i have been interested in trying for myself since i first read that thread of una's.
if u read the above thread u will see that una used BAP and lanolin to produce a paste similar to the one i am using, so its not exactly the same, mine is a lower tech attempt to produce similar results, he talks about keiki paste there so i thought it would be worth a shot.

below are the 12 test specimens.
u can see from the photos that i have smeared the paste on one aerole per specimen, using a tooth pick.

#1. astrophytum asterias.


#2. myrtillocactus geometrizans monstrosus, aka booby cactus.


#3. lophophora williamsii cristata.


#4. trichocereus pachanoi cristata.


#5. four ribbed trichocereus bridgesii.


#6. myrtillocactus geometrizans.


#7. trichocereus bridgesii monstrosus, aka penis plant.


#8. trichocereus peruvianus.


#9. trichocereus pachanoi "tom juuls giant"


#10. lophophora williamsii A.


#11. lophophora williamsii B.


#12. lophophora williamsii C.



im not doing this in an attempt to produce wierd pupping lophophora or multi branched trichocereus, it is simply a test to determine its effectivity.
if it is succesful i shall be using it as a means to propagate rare specimens.
it should provide a good alternative method of producing pups for grafting, as opposed to mutilating specimens that i dont want to disfigure.

i'll report back in a month or so and hopefully i will have some results to show *fingers crossed*
so wish me luck cos this could be very useful to all the cacti growers out there :yesnod:


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Offlinefelixhigh
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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7507157 - 10/10/07 11:20 PM (7 years, 2 months ago)

Very nice, good luck!
My friend is trying to make a BAP paste... No success so far, I believe he didn´t make it strong enough.


FH


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OfflineDr. uarewotueat
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Re: Cytokinin Experiment. [Re: felixhigh]
    #7507194 - 10/10/07 11:29 PM (7 years, 2 months ago)

yeah im not sure that the keiki paste has enough hormone in it, but lets hope so!


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InvisibleVirgilKane
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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7507230 - 10/10/07 11:36 PM (7 years, 2 months ago)

Cool!  Very interesting experiment.  Did you get the paste from an Orchid grower who made it themselves or is it commercially available?

Good luck!:cool:


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Offlinekadakuda
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Re: Cytokinin Experiment. [Re: VirgilKane]
    #7507338 - 10/11/07 12:16 AM (7 years, 2 months ago)

sweet man looks great! cant wait to see what happens with the astrophytum! the crest will be quite interesting as well. What % is your paste? my brother in law bought a new brand a while ago and it is very weak and virtually useless (for orchids).....the cheapskate lol.

virgil, google it. its easy to find online. you can also buy all the ingredients to make your own (if you want to put in the effort and money)


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InvisibleVirgilKane
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Re: Cytokinin Experiment. [Re: kadakuda]
    #7507343 - 10/11/07 12:18 AM (7 years, 2 months ago)

Cool man, thanks!


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Absense of evidence is not evidence of absense...

"Religion is a defense against a religious experience"
              Carl G. Jung

 
"So really, ordinary reality is a kind of chemical habit, sanctioned by culture, which says it's okay to use certain drugs, eat certain foods, and have certain sexual behaviors. However, when you transcend all this pre-conditioning by returning to the original wisdom of the animal body, then you discover this immense dimension of opportunity. For some people, it is a frightening risk. To me, that's the psychedelic experience."
Terence McKenna


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Re: Cytokinin Experiment. [Re: VirgilKane]
    #7507800 - 10/11/07 05:12 AM (7 years, 2 months ago)

did you cut the point of where you applied the paste?

i know when i did orchids a few years ago that it came with a little knife and i had to cut a little flap under the node then spread it.


maybe cut a tuff off one and give it a try??


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Invisiblethallus
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Re: Cytokinin Experiment. [Re: VirgilKane]
    #7507802 - 10/11/07 05:20 AM (7 years, 2 months ago)

Great stuff, do you know which type of CK is in your paste?


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OfflineDr. uarewotueat
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Re: Cytokinin Experiment. [Re: royer]
    #7507814 - 10/11/07 05:26 AM (7 years, 2 months ago)

Quote:

kadakuda said:
What % is your paste?  my brother in law bought a new brand a while ago and it is very weak and virtually useless (for orchids).....the cheapskate lol.



i have no idea on the % of cytokinin in the paste, it wasnt quoted...
if it doesnt work then i will buy the BAP and lanolin and make up my own paste as in una's thread...
i just wanted to try this as its alot easier and cheaper than making my own :wink:

plus some fuckstick stole my orchids so i have no other use for it...


Quote:

royer9864 said:
did you cut the point of where you applied the paste?

i know when i did orchids a few years ago that it came with a little knife and i had to cut a little flap under the node then spread it.


maybe cut a tuff off one and give it a try??



yeah with orchids u have to remove the bract from the node, then apply to the fresh exposed area...
i dont think that will be necessary with cacti though, i dont want to damage the aerole in any way or that will probably stop it from throwing a pup...
i did scrape the surface of the aerole lightly with the toothpick, to make sure that the paste was well applied :yesnod:


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Offlinejasonpwnd
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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7508160 - 10/11/07 11:07 AM (7 years, 2 months ago)

sounds very interesting, cant wait to see your results


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Offlinegeedorah
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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7508205 - 10/11/07 11:31 AM (7 years, 2 months ago)

Those phals look sexy on that wiki page. I'm going to give this a try on some of my orchids.

Great experiment!


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OfflineDr. uarewotueat
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Re: Cytokinin Experiment. [Re: geedorah]
    #7508212 - 10/11/07 11:33 AM (7 years, 2 months ago)

cant believe someone stole my phals... i was gutted :crankey:


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Offlineroyer
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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7508229 - 10/11/07 11:42 AM (7 years, 2 months ago)

i will post pics tonight of a similar experiment and i would love to post it here if it is ok with you :-))


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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7508566 - 10/11/07 01:31 PM (7 years, 2 months ago)

Why not try on some cut aeroles and on some intact ones at the same time?

This can give you the best of both worlds,plus a more conclusive experiment.


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Re: Cytokinin Experiment. [Re: Psiloman]
    #7508577 - 10/11/07 01:32 PM (7 years, 2 months ago)

that is what i plan on doing :-)

just waiting to see if my friend minds me posting in his thread ??


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OfflineDr. uarewotueat
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Re: Cytokinin Experiment. [Re: Psiloman]
    #7508846 - 10/11/07 02:58 PM (7 years, 2 months ago)

Quote:

Psiloman said:
Why not try on some cut aeroles and on some intact ones at the same time?




one step at a time eh... i dont like mutilating my cacti for no reason.
as proved by una there is no need to cut the aerole if the hormone is of a sufficient strength...
if i have no success this way i shall perhaps try cutting the aerole... but more likely i will get my hands on some better cytokinin.


Quote:

royer9864 said:
i will post pics tonight of a similar experiment and i would love to post it here if it is ok with you :-))




go for it! :yesnod:


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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7510009 - 10/11/07 08:24 PM (7 years, 2 months ago)

ok so first here is the pic of the hormone
it is green and very thick like grease:-))


ok so first i took one of my lophophora's and cut off one of the aeroles off and applied

next i took a similar lophophora and applied with out cutting it


then i took a graft that i recently made and applied some to an aerole


then just for fun i took some pereskiopsis and plucked off a few leafs and applied some hormone, but it is not like it need a hormone to pup like crazy


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OfflineDr. uarewotueat
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Re: Cytokinin Experiment. [Re: royer]
    #7511509 - 10/12/07 03:47 AM (7 years, 2 months ago)

goodluck with yours man :thumbup:


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Re: Cytokinin Experiment. [Re: Dr. uarewotueat]
    #7511777 - 10/13/07 02:08 PM (7 years, 2 months ago)

:cool:


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OfflineDr. uarewotueat
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Re: Cytokinin Experiment. [Re: royer]
    #7622447 - 11/11/07 05:33 PM (7 years, 1 month ago)

hello :grin:



havent noticed any pups staring on the others yet, so i wont take this as conclusive proof that the keiki paste works, as this particular plant is clump forming anyway... but its a start :yesnod:

anything yet royer?


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