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OfflineASD
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Cloudy honey LC
    #5706923 - 06/03/06 09:32 AM (17 years, 9 months ago)

My honey water is cloudy, there are little fluff balls that I assume are myclym. The water is very milky looking, thick to the point of the fluff balls almost blending in. Is this normal?

Some of my other honey jars have fluff balls aswell but the balls seem to have sediment specs in them, is this fine or are these jars contamed?

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InvisibleAtheist
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Re: Cloudy honey LC [Re: ASD]
    #5706926 - 06/03/06 09:34 AM (17 years, 9 months ago)

Mine had a sediment speck in it and it didnt do anything. If you sterilized the LCs good and were sterile during inoculation then you should be fine.

The white cloudy shit is mycelium dude, unless you mean its 'milky' right out of the PC? dunno i've never done honey

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OfflineASD
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Re: Cloudy honey LC [Re: Atheist]
    #5706934 - 06/03/06 09:39 AM (17 years, 9 months ago)

it has just gotten cloudy since the myclem started spreading. When I shake the jars it looks as if there is a white cloudy inside (besides the myclem puffs)

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InvisibleMrMaddHatter
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Re: Cloudy honey LC [Re: ASD]
    #5706939 - 06/03/06 09:42 AM (17 years, 9 months ago)

Try shooting a small bit into a grain or pf jar and see if its any good. :thumbup:

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OfflineASD
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Re: Cloudy honey LC [Re: MrMaddHatter]
    #5706968 - 06/03/06 10:03 AM (17 years, 9 months ago)

i'll try, thanks.

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OfflineBPJohnny
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Re: Cloudy honey LC [Re: MrMaddHatter]
    #5706971 - 06/03/06 10:05 AM (17 years, 9 months ago)

Quote:

MrMaddHatter said:
Try shooting a small bit into a grain or pf jar and see if its any good. :thumbup:




:thumbup: :thumbup: test jar :smile:

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OfflineOatman2000
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Re: Cloudy honey LC [Re: BPJohnny]
    #5706982 - 06/03/06 10:16 AM (17 years, 9 months ago)

yeah mine got cloudy... take a look at my gallery


like little snowflakes...


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine

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InvisibleMorelman
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Re: Cloudy honey LC *DELETED* [Re: ASD]
    #5706987 - 06/03/06 10:19 AM (17 years, 9 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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OfflineBMArts
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Re: Cloudy honey LC [Re: Morelman]
    #5707494 - 06/03/06 01:30 PM (17 years, 9 months ago)

Cloudy LC is ime due to bacteria... if all goes well and your LC isn't contammed your LC will become more clear as the myc grows due to all the particles being brought together by the myc ataching to it... a cloudy milky apearence that starts happening and stays even with myc there is most definetly bacteria...


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Everything I post on this board is pure fiction. Nothing in the post above is real. It is all made up...


May the source be with GNU

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Invisiblemonstermitch
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Re: Cloudy honey LC [Re: BMArts]
    #5707580 - 06/03/06 02:08 PM (17 years, 9 months ago)

Quote:

BMArts said:
Cloudy LC is ime due to bacteria... if all goes well and your LC isn't contammed your LC will become more clear as the myc grows due to all the particles being brought together by the myc ataching to it... a cloudy milky apearence that starts happening and stays even with myc there is most definetly bacteria...




my experience states the same thing.
I hate cloudy.

Always use new jars when making LC.
Bacterial endospores can certainly survive a 15 minute pc at 15 psi. If there was ever bacteria in an old jar, the new LC will cloud up.


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InvisibleCureCat
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Re: Cloudy honey LC [Re: monstermitch]
    #5707951 - 06/03/06 04:33 PM (17 years, 9 months ago)

Wait up- if the mycellium continues to grow, it would suggest that no bacteria is present. As i have read and experienced, bacteria stunts growth. If bacteria is present, the mycellium should quit growing.


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OfflineASD
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Re: Cloudy honey LC [Re: CureCat]
    #5708066 - 06/03/06 05:13 PM (17 years, 9 months ago)

so if there is bacteria myclem stops?

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InvisibleCureCat
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Re: Cloudy honey LC [Re: ASD]
    #5708197 - 06/03/06 05:47 PM (17 years, 9 months ago)

Such as i have read and experienced with bacillus(wet spot), yes.


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InvisibleMorelman
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Re: Cloudy honey LC *DELETED* [Re: BMArts]
    #5709699 - 06/03/06 11:59 PM (17 years, 9 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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InvisibleCureCat
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Re: Cloudy honey LC [Re: Morelman]
    #5710507 - 06/04/06 08:58 AM (17 years, 9 months ago)

Hmmm, interesting.  When you say the contaminated culture has mush better growth, do you mean that it had better growth before the contam or that after contamination the mycellium picked up momentum?? 

I wonder which kind of contaminant commonly afflicts LC's as well... perhaps it is not bacillus, and that stunted growth is specific to bacillus but not other bacteria.  Or maybe it is not even a bacterial infection?

Nice pics, good reference.  :smile:


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InvisibleMorelman
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Re: Cloudy honey LC *DELETED* [Re: CureCat]
    #5711734 - 06/04/06 04:05 PM (17 years, 9 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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InvisibleCureCat
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Re: Cloudy honey LC [Re: Morelman]
    #5712033 - 06/04/06 05:32 PM (17 years, 9 months ago)

Awesome.  Keep us posted  :smile:


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InvisibleMorelman
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Re: Cloudy honey LC *DELETED* [Re: CureCat]
    #5712994 - 06/04/06 10:00 PM (17 years, 9 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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Offlinekilroy
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Re: Cloudy honey LC [Re: Morelman]
    #5713429 - 06/04/06 11:57 PM (17 years, 9 months ago)

I had an LC that had bacteia in it and they grew in perfect purportion to each other until all the nutes were used up then the bacteria kept going.


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IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.




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Invisiblefastfred
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Re: Cloudy honey LC [Re: kilroy]
    #5713616 - 06/05/06 12:41 AM (17 years, 9 months ago)

> The water is very milky looking, thick to the point of the fluff balls almost blending in. Is this normal?

No, it's bacterial or yeast contamination. Throw it out.

> Try shooting a small bit into a grain or pf jar and see if its any good.

Bad idea. It's a 99% chance of a wasted jar. If it's cloudy, it's contaminated.

> Q: Does the presence of bacteria stimulate mycelium growth.

Interesting question. Some types of bacteria probably do. It could be useful if one could be found and studied.

> Wait up- if the mycelium continues to grow, it would suggest that no bacteria is present.

Not really. Only if the bacteria is parasitic or produces a toxin. Otherwise it's just competing for nutes. That shouldn't be a problem right away, of course bacteria will far out colonize the liquid media and end up causing the myc to stop prematurely.

> A few days ago I decided to add stones to my Karo LC's. I microwaved the stones in water for 5 minutes B4 dropping them in.

Why would you think that 5 min in a microwave would sterilize them?

> 1) Could be free-floating mycellium causing the cloudiness.

No. Mycelium forms visible sized networks. Single celled organisms cause cloudiness.

> It could have absorbed an unknown nutrient(s) which could be leaching out and causing the wild growth.

Highly unlikely.

> ...it can be isolated and studied, It could be an important advance for mycology.

True. But your jars are way to young to tell if the bacteria has any good effect. If it stimulates growth for a little while and then kills the myc it won't be too useful.

> "Pseudomonas putida, Bacillus megaterium, Azotobacter vinelandii and others have all been shown to have stimulatory effects on various mushroom species~either in the germination of spores, the growth of mycelia or the formation of fruitbodies."

Awesome info. I wonder if anyone's tried using this to their advantage.


-FF


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It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid

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Offlinehippie_cune
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Re: Cloudy honey LC [Re: Oatman2000]
    #5755971 - 06/16/06 01:05 AM (17 years, 9 months ago)

Quote:

Oatman2000 said:
yeah mine got cloudy... take a look at my gallery


like little snowflakes...




what are those balls in the jar?

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InvisibleMorelman
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Re: Cloudy honey LC *DELETED* [Re: hippie_cune]
    #5755982 - 06/16/06 01:09 AM (17 years, 9 months ago)

Post deleted by Morelman

Reason for deletion: Never again...



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Offlinehippie_cune
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Re: Cloudy honey LC [Re: Morelman]
    #5756002 - 06/16/06 01:19 AM (17 years, 9 months ago)

....?

what are those for?
what else can be used?

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Offlineetam
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Re: Cloudy honey LC [Re: hippie_cune]
    #5756073 - 06/16/06 01:49 AM (17 years, 9 months ago)

The marbles are used as an agitator to break up the mycelium, especially when you want to suck it up into a syringe and inoculate some cakes.

You just jiggle the jar and the marbles do their job.

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Offlinehippie_cune
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Re: Cloudy honey LC [Re: etam]
    #5756091 - 06/16/06 01:55 AM (17 years, 9 months ago)

ahh.. i see.

i assume thats done with the boiling steps eh?

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Offlineetam
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Re: Cloudy honey LC [Re: hippie_cune]
    #5756100 - 06/16/06 01:59 AM (17 years, 9 months ago)

Well.. you insert the marbles during the 'boiling' or 'PC' steps so that it's all sterile...

You should just check out http://www.shroomery.org/wiki/pmwiki.php/Main/LiquidCulture and do some research.

Morelman also has a nice Karo LC tek in his signature.

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