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 captmueller
chemical shaman
Registered: 10/25/04
Posts: 115
Last seen: 6 years, 3 months
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 Re: :LIQUID CULTURE - THE EASY WAY:  [Re: GnuBobo]
#4149114 - 05/08/05 03:37 AM (8 years, 15 days ago) |
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i use the ultra black and it seems to make good selfhealing injection ports...
namaste,
captmueller
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc: 
Last seen: 4 months, 12 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: VALIS]
#4150951 - 05/08/05 08:37 PM (8 years, 14 days ago) |
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Quote:
VALIS said:
Quote:
agar said:
LOL...... In another life... I posted here as 6T. agar=SixTango
You should have mentioned that when I originally made the above post ... guess maybe you must have missed it.
People are funny.
Thats why I was making the comments about 6T not knowing what he was doing  Hey who gave this thread a three. Goobers.
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Blue Helix
old hand
Registered: 02/02/03
Posts: 1,101
Last seen: 6 days, 2 hours
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4151489 - 05/08/05 11:21 PM (8 years, 14 days ago) |
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Quote:
You now have a sterilized nutrient solution, in which to inject 2 or 3 ml/cc of spore solution into.
Why so much spore solution? Using a 1/2cc syringe with a 29-gauge needle, I inoculated agar half pints with spore solution. Each jar received only about 100 microliters (about one drop) of clear spore solution, yet those agar surfaces blew up with mycelium in only 3 days. At the end of one week, most surfaces were 60% covered or more!
The point is that if a single 100 microliter drop of spore solution can colonize an entire half pint agar surface in a few days which can then easily make 200 ml of liquid culture when blended, then why use 100X that amount of spore solution for a small 200ml jar of liquid culture? It's wasteful of the spore solution. I wouldn't use over 1/4 cc of spore solution myself. That should be MORE than enough.
Edited by Blue Helix (05/28/05 02:52 AM)
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 @cro
new name
Registered: 12/07/02
Posts: 1,224
Loc: The PNW
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4152465 - 05/09/05 05:02 AM (8 years, 14 days ago) |
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 You're 6T?
Damn, I still have a bottle of your old liquid nutrient solution. 
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Blue Helix]
#4152603 - 05/09/05 09:01 AM (8 years, 13 days ago) |
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You dont need to inject 2 or 3 ml of spore solution. Any amount (so long as it has a few viable compatable spores in it) will work. More - simply gets the action going faster, IMHO.
I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.
Once - you master mush/cult & PRINT or SPORE SOLUTION MAKING you generaly have more spores in print or solution form - than a single person (or small group) could EVER USE.
I often use a SPORALATOR to make spore solution:
 
1/2 or 1 gallon wide mouth jar (Sun Tea type) - with spigot removed & replaced with an injection / asperation port - that reseals, installed in the hole in the jar - where the spigot was.
Lid altered with a gas exchange filter & install a clip of some sort to hold a cap suspended with a stainless steel treble hook through the caps stem area - inside the container.
Fill container with 1000 / 1500 / 3000 ml water (depending on size of container) & PC the whole container with water inside it 20 minutes @ 15 psi & allow to cool to room temp.
When ready for use, harvest a large cap (veil about ready to tear), inside a G/B or F/H - swab cap top with h202, remove veil, peirce cap stem area with sterile treble hook, open container & attach cap to clip, which hangs over sterile water & will drop spores directly into it (repeat once or twice or more w/same strain cap).
The result is 1000 / 1500 / 3000 ml of spore solution that can be asperated through the injection / asperation port on the side of the jar via a sterile syringe.
Test first syringe on agar/petri/plate to insure spore solution contains no contaminates & (if not) presto - you now have a capacity to fill 100 / 150 / 300 syringes out of a single spore laden solution container - in aseptic conditions. 
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,898
Last seen: 3 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4152692 - 05/09/05 10:03 AM (8 years, 13 days ago) |
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Agar....you're crazy lol
Killer "Spore-A-Lator" btw
-Gnostic
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Quote:
IGnosticAbhorI said:
Agar....you're crazy lol Killer "Spore-A-Lator" btw
-Gnostic
Just a FUN hobby.........   
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Blue Helix
old hand
Registered: 02/02/03
Posts: 1,101
Last seen: 6 days, 2 hours
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4154692 - 05/09/05 09:09 PM (8 years, 13 days ago) |
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Quote:
I certainly don't advocate WASTING spore solution. At the same time, I prefer to use the content of a spore laden syringe at one sitting, so I do not have partial syringes laying around.
Ah, but you, Agar, are the one who has shown us the secret of using vacutainers to store spore solution...
That way you don't leave you spore solution lying around at all. You just poke the needle through the rubber lid thingy, draw out what you need (say 1/4th CC), and the rest is kept sterile and perfect in the vacutainer.
Edited by Blue Helix (05/09/05 09:14 PM)
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Silly_Cyben
not-quite-newbie
Registered: 04/11/05
Posts: 183
Loc: USA
Last seen: 5 years, 2 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4155749 - 05/10/05 02:17 AM (8 years, 13 days ago) |
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Sounds great. Thanks for sharing this.
How long can these bottles be stored in the refrigerator?
William
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down44
Johnson
Registered: 12/02/04
Posts: 177
Last seen: 2 months, 30 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Silly_Cyben]
#4155886 - 05/10/05 02:49 AM (8 years, 13 days ago) |
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great tek
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Silly_Cyben]
#4156485 - 05/10/05 09:33 AM (8 years, 12 days ago) |
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Quote:
browilliam said: How long can these bottles be stored in the refrigerator? William
Once you have healthy culture growth, it is BEST to stall them, by placing in the fridge - where they can/will last several months.
1 gallon LC on a magnetic stir plate
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ticktock
Seeker
Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 3 years, 2 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4163793 - 05/11/05 10:32 PM (8 years, 11 days ago) |
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My 2 cent additional suggestion;
Do everything just as described above. After you see the first development of mycelium, inject a volume of 3% peroxide equal to 1/20 the volume of the liquid culture. Within a minute, the mycelium will head for the surface and float. They have caught tiny bubbles of oxygen and will use it up in the next few days. Repeat when they sink again.
There you have aeration without the need for super filtered air.
ticktock
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 Don't panic!
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davesj1
Stranger
Registered: 04/11/05
Posts: 247
Last seen: 1 year, 7 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: agar]
#4174564 - 05/14/05 02:45 PM (8 years, 8 days ago) |
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can gerber bottles stand up to pressure cooking?
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RootedGrowth
Grain Apprentice
Registered: 10/30/04
Posts: 225
Last seen: 7 years, 7 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: davesj1]
#4174626 - 05/14/05 03:07 PM (8 years, 8 days ago) |
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nice tek and a tag
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Death is a reason to live
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,898
Last seen: 3 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: davesj1]
#4174639 - 05/14/05 03:12 PM (8 years, 8 days ago) |
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Quote:
davesj1 said:
can gerber bottles stand up to pressure cooking?
Read the thread, that's what it's there for, this question has already been anwsered...
-Gnostic
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CLUTCH
Stranger
Registered: 04/11/05
Posts: 879
Last seen: 4 months, 18 days
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Well, actually the tek is using EvenFlow bottles, but yea dave, the Gerber bottles will work just as good.
btw nice find there gnostic (in your avatar) I would love to find that growing in my backyard someday. 
Edited by CLUTCH (05/14/05 09:10 PM)
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blackout
Microwaver
Registered: 07/17/00
Posts: 3,356
Last seen: 1 year, 4 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: backupwards]
#4177482 - 05/15/05 10:54 AM (8 years, 7 days ago) |
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Quote:
backupwards said:
blackout like the little pic in your sig, how long did it take you to sit and put that cow together?
I stole it from another message board! I did the pathetic attempt of the shroom behind him myself.
I have some LC with just glucose growing at the moment, it is not as active as my honey water but it is clearer to see growth. I may try some regular sugar (sucrose) to see if it works, most say it won't but I have never seen somebody try it.
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Abermelin
Gnome Hat
Registered: 03/22/05
Posts: 394
Loc: In The Mycelial Network
Last seen: 8 years, 2 days
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: blackout]
#4177567 - 05/15/05 11:36 AM (8 years, 7 days ago) |
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at what point do we feed this solution to a baby?
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vescudero0
ay ay aaaaay
Registered: 01/28/05
Posts: 418
Last seen: 6 years, 6 months
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: Abermelin]
#4178030 - 05/15/05 01:30 PM (8 years, 7 days ago) |
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very good info, thanx once again---
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live & enjooooooooooooooooy
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kake
The answer to1984 is 1776.
Registered: 05/07/99
Posts: 2,782
Loc: The 66th harmonic
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Re: :LIQUID CULTURE - THE EASY WAY: [Re: vescudero0]
#4178780 - 05/15/05 05:01 PM (8 years, 7 days ago) |
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VALIS: how do you keep the polyfill from getting soaked while PCing?
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The answer to 1984 is 1776.
Edited by kake (05/17/05 12:18 PM)
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