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 Workman
Psilocybe Microscopist
 
Registered: 03/01/01
Posts: 3,094
Loc: Washington, USA
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 Panaeolus cyanescens, Suphanburi Thailand - robust isolate
#3751402 - 02/08/05 02:16 PM (7 years, 3 months ago) |
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Panaeolus cyanescens from Suphanburi, Thailand on cased sterile horse manure processed in a presealable injection port mycobag. Very easy to deal with since you can seal before sterilization without fear of rupture.
This strain tends to produce particularly large well formed fruits quickly in culture. Pinhead coloration is a brownish olive that fades to greyish white with maturity. Spore production is heavy over a long period of time. Flesh blues intensely when cut or bruised.
1st flush 7 days after casing with a thin layer of sterile peat and calcium carbonate
2nd flush, the largest mushroom has a cap diameter over 4 centimeters (1.5 inches) and is not fully expanded.
2nd flush long shot.
Close up of gill surface showing basidia with spores. Pointer is approximately 8.4 micrometers in width.
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 Arp
roving mycophagist
Registered: 04/20/98
Posts: 2,191
Loc: in a van by the river
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3751415 - 02/08/05 02:21 PM (7 years, 3 months ago) |
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Nice! Potency-wise are the Pan Cyanescen strains simular as with the cubes? 
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,094
Loc: Washington, USA
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Arp]
#3751421 - 02/08/05 02:22 PM (7 years, 3 months ago) |
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I've never heard of a weak pan, but I am sure they vary a bit between strains.
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Fluxburn
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3751601 - 02/08/05 03:00 PM (7 years, 3 months ago) |
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damn I want a print
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,094
Loc: Washington, USA
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3756728 - 02/09/05 02:35 PM (7 years, 3 months ago) |
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Images from today
Grid in the background is 1/2 inch per square. The largest mushroom is nearly 2 inches across which is huge for Panaeolus cyanescens. I need to check the maximum recorded size in nature. I should mention that the original specimen was collected by MJShroomer and at the time it was not identified with certainty. The original specimen was not large or impressive but was noted as unusual and therefore collected and kept seperate for later identification. Cultivated specimens are definately Panaeolus (Copelandia) but the species has yet to be determined with certainty. It is probably merely a large form of P. cyanscens.
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Edited by Workman (02/09/05 02:45 PM)
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Psilygirl
cyan goddess
Registered: 08/28/03
Posts: 4,418
Loc: PNW
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3756782 - 02/09/05 02:42 PM (7 years, 3 months ago) |
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absolutely gorgeous  keep up the good work!!
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onetime
onetime
Registered: 11/13/03
Posts: 3,609
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Psilygirl]
#3757320 - 02/09/05 04:08 PM (7 years, 3 months ago) |
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nice
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3757376 - 02/09/05 04:14 PM (7 years, 3 months ago) |
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NICE.
4-spored!!
Got any clear pics of the pleurocystidia, and sizes?
The domestic hawaiian in circulation can produce some woopers like that, but typically they are shorter when the caps are that big.
Very nice. 
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bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: EonTan]
#3758491 - 02/09/05 07:27 PM (7 years, 3 months ago) |
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By posting the basidia i assume your suggesting that it is Pan.cyanescens, since Pan.trops and Pan.cambo are both reported to have about 50% 2-spored basidia, and this mushroom clearly has four spored basidia consistantly from your picture.
as for cystidia - the range of cystidia displayed in Panaeolus cyanscens is so huge that had Guzman worked on the species im sure he would have delineated it into about 6 new species all identified only microscopically and of redudant value...
Very nice looking pans there - very yum looking too -
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 5,142
Loc: N38.93829W119.98108
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: bluemeanie]
#3758629 - 02/09/05 07:49 PM (7 years, 3 months ago) |
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boyyyy. Those sure do look nice! 
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The subjects that I cover are not species specific and can be used in All Aspects of Mycology
I really need a good flow hood and a scope
Teh=The
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,094
Loc: Washington, USA
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: EonTan]
#3761343 - 02/10/05 10:07 AM (7 years, 3 months ago) |
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Pleurocystidia: pointer is 8.4 micrometers in width.
The maximum published cap diameter for Panaeolus cyanescens and is 4 centimeters. The largest mushroom (so far) pictured above is just over 5 centimeters.
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Reinvesting 25% of Sales Towards Basic Research and Species Identification
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3761595 - 02/10/05 11:00 AM (7 years, 3 months ago) |
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Thanks.
The other asiatic Copelandias in circulation have small pleurocystidia too. Is that pointer 8.4 microns calibrated with a stage micrometer?
Very nice size fruits!!
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,094
Loc: Washington, USA
Last seen: 15 hours, 15 minutes
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: EonTan]
#3761729 - 02/10/05 11:26 AM (7 years, 3 months ago) |
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Quote:
Is that pointer 8.4 microns calibrated with a stage micrometer?
Yes, but I don't have a stage micrometer that measures in single microns. I had to use a 10 micron divison scale and digitally divide it into smaller units. But as best as I can tell the pointer is very close to 8.4 microns (microns is the old term, micrometers is now considered more correct. But I still like the sound of microns myself).
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Research funded by the patrons of
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Reinvesting 25% of Sales Towards Basic Research and Species Identification
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ralphster44
Vendor/collector
Registered: 01/03/01
Posts: 4,579
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3761811 - 02/10/05 11:43 AM (7 years, 3 months ago) |
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.
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Edited by ralphster44 (02/16/05 12:11 PM)
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Workman
Psilocybe Microscopist
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Posts: 3,094
Loc: Washington, USA
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: ralphster44]
#3761912 - 02/10/05 12:07 PM (7 years, 3 months ago) |
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You will have to excuse me if I don't take your advise and ignore your predujice against bags. You are also showing your ignorance with your evaporation rate comment. As can be clearly seen, the bag has been cut away to allow casing and fruiting. The bag in no way hampers evaporation rate from the surface any more than a rigid tray might. It may not have been clear in my earlier post, but I have been working with this strain for quite awhile. Here is my first attempt with the wild culture which had to be extracted from a dried mushroom.
Dried mushroom (not very impressive)
Small tupperware container test fruiting.
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Research funded by the patrons of
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Anno
Experimenter
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 Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3762057 - 02/10/05 12:35 PM (7 years, 3 months ago) |
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They look great! I had nothing but bad luck with the recent panaeolus attempts  , but these pictures make me want to get a fresh start again. 
From my previous grows I had the feeling that an addition of straw is necessary to achieve good sized fruits, but it seems that the most factors still lie in the genes, as your horse dung grow shows.
Great work!
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,380
Loc: The Matrix
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 Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Anno]
#3762263 - 02/10/05 01:17 PM (7 years, 3 months ago) |
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I know that horse dung rocks!!!
Workman....excellent results. I am anxious to obtain a print from this species/strain.
Joshua
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Shdwstr
FSRCanada
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3762413 - 02/10/05 01:45 PM (7 years, 3 months ago) |
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Yes... absolutely beautiful work
When I grow up, I wan't my Pans to look as good
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dmc_
211
Registered: 12/23/03
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Shdwstr]
#3764148 - 02/10/05 07:06 PM (7 years, 3 months ago) |
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Constructive critizism is one thing, but I feel that r44 kind of attacked you alittle on his comment workman. Awesome grow!
www.sporeworks.com,
^great people
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 5,142
Loc: N38.93829W119.98108
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: ralphster44]
#3764232 - 02/10/05 08:21 PM (7 years, 3 months ago) |
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Quote:
ralphster44 said:
I guess you will never know the potential using BAGS however.
I'll post the full possibilities I get with this strain, when done properly with a casing.
It's a shame you used a bag...
Staw away from the Bags Workman...
Wtf?? i doupt he is growing for quanity
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The subjects that I cover are not species specific and can be used in All Aspects of Mycology
I really need a good flow hood and a scope
Teh=The
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