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 Workman
Psilocybe Microscopist
 
Registered: 03/01/01
Posts: 3,058
Loc: Washington, USA
Last seen: 4 hours, 28 minutes
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 Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: tregar]
#3857371 - 03/02/05 12:17 PM (6 years, 11 months ago) |
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I used special presealable bags with injection port. They are more expensive but I get them free for my efforts. They have a special filter that vents faster to prevent bursting.
I didn't use a recipe. It is just horse manure, mostly dung with some grass or straw mixed in (not added but present in the manure when gathered). It was pretty wet when I got it so I squeezed out as much water as I could and then broke it up into a fluffy texture. I then loaded the bags, impulsed sealed them closed and sterilized at 15psi for 2 hours.
Earlier I had made a dilute malt water broth (1tsp:2cups) with a bit of the manure water from the squeezing. Sterilized this for 20 minutes at 15psi and inoculated with a pan cyan agar wedge when cool. This liquid inoculate was colonized in just a few days and was ready to draw into syringes and inject into the bags. I injected a full 10cc into the manure bag and growth was noticable in 24 hours at 85F. 10 days later (with one shaking) the bag was ready to be cased and 7 days later the first mushrooms were harvested.
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tregar
Stranger
Registered: 08/30/04
Posts: 111
Last seen: 6 days, 10 hours
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: Workman]
#3864176 - 03/03/05 05:20 PM (6 years, 11 months ago) |
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thanks for explaining, great job.
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psiloz
Stranger
Registered: 06/03/03
Posts: 75
Loc: Australia
Last seen: 6 years, 9 months
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Re: Panaeolus cyanescens, Suphanburi Thailand - robust isolate [Re: tregar]
#4049972 - 04/13/05 10:21 AM (6 years, 9 months ago) |
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Those robust Pans look just like the big var of pans in the fields of nthn NSW
identical in size and stature
could be the same strain/species
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