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Anonymous

Sterilizing with Heat or Fire, versus wiping with alcohol (needle and others)
    #3625737 - 01/13/05 11:29 PM (9 years, 7 months ago)

All teks, every ware, recommend using heat. Burning the needle, heating the foil, etc? Can I use cotton and alcohol to sterilize the needle after every inoculation instead of burning it? It is pretty difficult to burn anything in an airtight glovebox, and dangerous if you sprayed Lysol on it.
I feel fire is just so dirty imperfect process that I wish I could clean everything inside the glovebox with alcohol.
Nobody mentions alcohol, is there anything wrong with it?
Can I clean with alcohol the foil that I?m going to use for the jars?


Edited by Anonymous (01/14/05 12:12 AM)


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InvisibleHolydiver
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Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: cuatiklad]
    #3625760 - 01/13/05 11:33 PM (9 years, 7 months ago)

Alcohol burner can be a cumbersome pain in the glove box, can't recall the last time I used mine for anything really. Plus, no one wants dirty burned needles like you mentioned.

Alchohol + a paper towel is all you need to sterilize between inoculation points - keep the needle covered in alcohol soaked paper towel between shoots.


--------------------
To find a place to live between the negatives and positives.


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Anonymous

Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: Holydiver]
    #3625903 - 01/14/05 12:16 AM (9 years, 7 months ago)

thanks
You had free me.


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Invisibleohmatic
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Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: cuatiklad]
    #3627491 - 01/14/05 08:20 AM (9 years, 7 months ago)

jep.

but for initial sterilisation, before you can swab it,
you shall flame it till glowing red because alcohol cannot kill any contams
that may be lurking in the inside of the needle waiting to grow.

by flaming it, you also toast these guys.
peace ohm :mushroom2:


--------------------
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RIP #cultivation! ....can't associate? well FUCK U !


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OfflineMycoJunkie
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Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: ohmatic]
    #3627688 - 01/14/05 11:35 AM (9 years, 7 months ago)

I wipe my syringe with 99.99% Germ killing Hand Sanitizer from any grocery or pharmacy store. Works everytime.

I also suggest Lysol Anti-Bacterial wet wipes. They sterilize shit good too.

IMHO, Flame sterilizing is a waste of time.


--------------------
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Invisibleohmatic
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Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: MycoJunkie]
    #3627714 - 01/14/05 11:48 AM (9 years, 7 months ago)

Quote:

MycoJunkie said:
IMHO, Flame sterilizing is a waste of time.




read my post again, if there is shit in the needle, ya fooked.
since alc cant go there.

also it takes like 10 secs to sterilize it with the flame,
WHAT an enormous ammount of time.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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OfflineKaptKid
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Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: ohmatic]
    #3628440 - 01/14/05 02:39 PM (9 years, 7 months ago)

Quote:

ohmatic said:
Quote:

MycoJunkie said:
IMHO, Flame sterilizing is a waste of time.




read my post again, if there is shit in the needle, ya fooked.
since alc cant go there.

also it takes like 10 secs to sterilize it with the flame,
WHAT an enormous ammount of time.
peace ohm :mushroom2:





Thats GOOD advice but experience is also a teacher.Just slower.GL


--------------------
Child of the 60's, Tripping ever since.


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InvisibleRockstar69
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Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: KaptKid]
    #3628506 - 01/14/05 03:00 PM (9 years, 7 months ago)

Dunno if this helps but..
After sterilizing, I fill my syringe cap with h-peroxide before snapping it back on.. it hasnt hurt any of my spore syringes yet  :smile:


--------------------
"We gotta stop smokin', stop, stop, ..I mean cigarette smokin!" -- Jimi Hendrix :jamming:


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Anonymous

Re: Sterilizing with hit, versus wiping with alcohol (needle and others) [Re: MycoJunkie]
    #3629096 - 01/14/05 05:56 PM (9 years, 7 months ago)

MycoJunkie :

That sound great, and so commutable, but I?m always afraid Lysol will hurt the spores. The ideal could be if one could wipe the needle and the lid with Lysol like substance, can this be done wit no risk?

Also, can you give me more detail of how you do it?
The soap you mention, is it liquid or bar soap?
Which one do you use and what do you use to wipe things? tissue or something?

Does any one else does this?

Thank you all for your imputs, it has really help
Clarify lots of doubts


Edited by Anonymous (01/14/05 05:59 PM)


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