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 absolute zero
The Hero
 
Registered: 11/04/01
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 Re: The Coffee Project [Re: Hippie3]
#3050806 - 08/25/04 08:52 PM (7 years, 9 months ago) |
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Well, lets aid the cause of experimentation...
Rye was simmered in solution (50/50 freshly brewed coffee to water) for 45 minutes
Approximately 1 cup of simmered rye was placed in each quart jar
Jars will sit overnight and be PC'd in the morning
Jars will be innoculated via G2G using a pre-existing, 100% colonized quart of rye (08/27)
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Edited by Zero__Glass (08/27/04 07:13 AM)
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absolute zero
The Hero
Registered: 11/04/01
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 Re: The Coffee Project [Re: Hippie3]
#3050830 - 08/25/04 09:00 PM (7 years, 9 months ago) |
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Also... another thought... if the point of not using coffee on top of casings is to not invite contamination, what about using that coffee mixture for a bottom casing layer or using that syringe of yours to inject coffee into the bottom layer of a casing that's on a second or third flush?
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 GnuBobo
Frilly Cuffs Extraordinaire
Registered: 06/17/04
Posts: 43,754
Loc: Charisma
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Re: The Coffee Project [Re: Hippie3]
#3073496 - 08/31/04 10:29 AM (7 years, 8 months ago) |
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So in grain preparation with coffee, we use 50% diluted coffee for the 24 hour soak. Then, for the simmering, do we also use 50% diluted coffee water? Or do we just use normal water for the simmering? It seems like simmering for 45 minutes or what have you in normal water would leach out the nutrients absorbed during the soak. Any comments on this?
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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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FirstAvailable
enthusiast
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Re: The Coffee Project [Re: GnuBobo]
#3073513 - 08/31/04 10:36 AM (7 years, 8 months ago) |
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I wuld say use the same water/coffe you used to soak the grain.
Why would you need to use fresh water to simmer?
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GnuBobo
Frilly Cuffs Extraordinaire
Registered: 06/17/04
Posts: 43,754
Loc: Charisma
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I like to drain the water off after soaking. It gets a little nasty. And haven't you just freed up loads of endospores into the water? I'd think you'd want to dump those, even if they are PC'd in the end.
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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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RogerRabbit
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Re: The Coffee Project [Re: GnuBobo]
#3077263 - 09/01/04 06:36 AM (7 years, 8 months ago) |
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No, I boil in the nasty coffee/water they soak in. Sometimes I let it go too long and it begins to ferment. That's ok too. After soaking 24-48 hours, only let it boil for five or ten minutes max. Pour out into a very large spaghetti strainer and let drain for a few minutes. Then, every few minutes or so, toss the grains around in the strainer by shaking it up and down. This will 'rotate' the rye and you'll see the steam rising off it. This is a good way to dry the grain before you load the jars. Keep tossing them until there's no more steam. That should leave them dry on the outside. Then, load your jars and pc.
It helps to use a pinch or two of hydrated lime in the mix to combat the acidity of coffee. It's also good to add about 1/2 tablespoon of gypsum per gallon, to the water the grains soak/boil in.
Edited by RogerRabbit (09/01/04 06:41 AM)
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GnuBobo
Frilly Cuffs Extraordinaire
Registered: 06/17/04
Posts: 43,754
Loc: Charisma
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THanks! Well, just today I have loaded up 7 quarts of wheat berries and they're soaking in coffee-water now. Will add a pinch of lime and shake the jars around while they soak. Good information, Roger. You are a huge help! A vault of knowledge, I daresay. I'll post pics of things if I see anything interesting (these will be 'nnoced up with some karo I made...not sure how robust this myc will be. Keeping fingers crossed.)
GB
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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!
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absolute zero
The Hero
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Quote:
Zero__Glass said:
Rye was simmered in solution (50/50 freshly brewed coffee to water) for 45 minutes
Approximately 1 cup of simmered rye was placed in each quart jar
Jars will sit overnight and be PC'd in the morning
Jars will be innoculated via G2G using a pre-existing, 100% colonized quart of rye (08/27)
One week later...
Jars have been colonized for days
Jars have received light exposure
Jars have yet to show signs of pinning
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suzie_girl
journeyman
Registered: 10/31/01
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Loc: The Proud and Ignorant So...
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This thread encouraged me to give it a try. Took two days of used coffee grounds (filters too) and mixed in a ziplock with a pint of colonized birdseed. You could really see the mycelia take off in the coffee. I'm sure the extra moisture helped. It was soon relatively well colonized.
Cased with Schultz Cactus Potting soil. Just getting my first pins, but lots of knots.
Coffee's great!
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megaman3
Stranger
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 Re: The Coffee Project [Re: suzie_girl]
#3100010 - 09/06/04 04:23 PM (7 years, 8 months ago) |
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Hippie3: Here is a question. Did the person who dunked his cake in cold coke for 24 hours (Baddaboom I believe) do anything after the dunk before putting the cake back in the jar for fruiting? I would assume that if one were to leave a cake that has been dunked in coke as is, then the coke on the surface would attract contams. So did he thoroughly rinse the cake after the dunk? Did he apply any peroxide to it after the dunk, or do anything else before putting it in the jar to fruit?
Also, since a cake is pretty porous, I would think that maybe it would be difficult to rinse all the exposed coke out (i.e. the coke that has not been absorbed by the mycelium and therefore is available to contaminant spores). Or, conversely, would you possibly get rid of all the coke by rinsing the cake too thoroughly, thus defeating the purpose of the dunk? Finally, did Baddaboom perhaps use a glovebox?
Thanks Hippie.
Edited by megaman3 (09/06/04 04:28 PM)
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absolute zero
The Hero
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Quote:
Zero__Glass said:
Quote:
Zero__Glass said:
Rye was simmered in solution (50/50 freshly brewed coffee to water) for 45 minutes
Approximately 1 cup of simmered rye was placed in each quart jar
Jars will sit overnight and be PC'd in the morning
Jars will be innoculated via G2G using a pre-existing, 100% colonized quart of rye (08/27)
One week later...
Jars have been colonized for days
Jars have received light exposure
Jars have yet to show signs of pinning
One week later, still waiting for invitro pins
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absolute zero
The Hero
Registered: 11/04/01
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Although I haven't seen results with invitro... After a casing started slowing down, weak coffee was injected into the bottom casing layer... 2-3 days later, the casing had a good number of mature fruits and a ton of pins... unfortunately, most of those pins ended up being aborts
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RogerRabbit
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Be sure to use an invitro proved isolated fruiting strain for stable results. If you have a good invitro producing strain, coffee will help it pin sooner. If not, no amount of coffee will make it pin invitro. For example, I've only seen half a dozen or so PE fruits form invitro, but tons of PR, BR, TX and Huatla.
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NNY
Z?
Registered: 07/17/04
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just reporting in... i just got back from a week and a half vacation, the cubes on coffee are fully colonized, 1 of the 4 was before i left. the pan cyans are still going painfully slow. one jar i caught a contamination in (rot) before leaving. the rest are colonized maybe 25% at most.
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Hippie3
mycotopiate
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Posts: 3,090
Loc: mycotopia.net
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Re: The Coffee Project [Re: megaman3]
#3178286 - 09/25/04 06:04 AM (7 years, 8 months ago) |
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Quote:
megaman3 said:
Hippie3: Here is a question. Did the person who dunked his cake in cold coke for 24 hours (Baddaboom I believe) do anything after the dunk before putting the cake back in the jar for fruiting? I would assume that if one were to leave a cake that has been dunked in coke as is, then the coke on the surface would attract contams. So did he thoroughly rinse the cake after the dunk? Did he apply any peroxide to it after the dunk, or do anything else before putting it in the jar to fruit?
Also, since a cake is pretty porous, I would think that maybe it would be difficult to rinse all the exposed coke out (i.e. the coke that has not been absorbed by the mycelium and therefore is available to contaminant spores). Or, conversely, would you possibly get rid of all the coke by rinsing the cake too thoroughly, thus defeating the purpose of the dunk? Finally, did Baddaboom perhaps use a glovebox?
Thanks Hippie.
he did rinse the cake
but that's all.
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Admin @ mycotopia.net
Mycotopia
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whiterasta
Day careobserver
Registered: 04/09/02
Posts: 1,780
Loc: Oregon
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Re: The Coffee Project [Re: Hippie3]
#3178409 - 09/25/04 07:55 AM (7 years, 8 months ago) |
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Gonna "claim" a new tek soon eh, Hippie 
WR
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To old for this place
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Anno
Experimenter
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Re: The Coffee Project [Re: Hippie3]
#3178413 - 09/25/04 07:58 AM (7 years, 8 months ago) |
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Hippie, are there any definitive reliable results in? Does coffee really help pinning? Is it significant?
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Silven
I trip,therefore weare.
Registered: 08/30/04
Posts: 2,071
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Re: The Coffee Project [Re: Anno]
#3191971 - 09/28/04 01:58 PM (7 years, 7 months ago) |
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So what I'm taking from all this reading (I read the entire thread) is this:
1) Coffee seems to enhance the growth of mycelium.
2) Coffee seems to increase the number of pins and amount of pins
*) Someone also claimed that they grew (6) 12" mushrooms off their 3rd and 4th flushes if I recall correctly
Here is where I would like Roger's feedback, or anyone who has been successful in duplicating his methods and feels knowledgeable about the "Coffee Project".
Would using 50/50 coffee/water mixture instead of straight water work for BRF cakes?
Every time I saw someone say they were trying it, I either passed their results unknowingly or they didn't ever post results.
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Also, my opinion is that the nutes in the coffee is what is speeding along colonization and pinning.
My reasoning is that if I break up a caff. pill and mix the appropriate amount to immitate the bottom of a cup of coffee, and leave that cup of water/caff. out, it will not mould. Atleast, I don't believe it will. I plan on trying this experiment.
My belief is it's the PH and the nutes that it provides. Mould seems to grow faster on a substance that has a higher acidity, and if this is the case that could explain why the mycelium does so well on coffee as well. Though, as we know too much acid is bad for mycelium, so what we should do now is dillute the PH little by little in controlled experiments.
Two people said the PH of coffee. One said 5, the other said 4.5. So what we should do is try 50/50 water/coffee mixtures with PH's of 5, 4.9, 4.8, etc all the way down to where it doesn't seem to do anything.
We should also target other suspected "growth boosters", like the caffiene that people were mentioning. I believe that decaf coffee still has some caffiene so one would need dichloromethane to rid it of the caff, and that can be rather costly (but attainable).
I'm not in the best state of mind, but I believe that what I said has atleast some value, if not to atleast weed out the PH factor.
Do with it what you will, but please answer the BRF question if nothing else! 
- Silven 
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Born: 10/31/83, which makes me a Scorpio.
1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.
What do you bring to the table?
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Lifenergy
Yo Mama
Registered: 08/05/04
Posts: 766
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Re: The Coffee Project [Re: Hippie3]
#3194558 - 09/29/04 03:34 AM (7 years, 7 months ago) |
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Has anyone tried just substituting coffee for water when making BRF/verm jars? I apologize if this has already been asked.
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Everything that we see is a shadow cast by that which we do not see.
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Silven
I trip,therefore weare.
Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
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Re: The Coffee Project [Re: Lifenergy]
#3194641 - 09/29/04 05:15 AM (7 years, 7 months ago) |
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I wrote:
Quote:
) Coffee seems to enhance the growth of mycelium.
2) Coffee seems to increase the number of pins and amount of pins
*) Someone also claimed that they grew (6) 12" mushrooms off their 3rd and 4th flushes if I recall correctly
Here is where I would like Roger's feedback, or anyone who has been successful in duplicating his methods and feels knowledgeable about the "Coffee Project".
Would using 50/50 coffee/water mixture instead of straight water work for BRF cakes?
Yea Lifenergy, I asked that yesterday, just awaiting an answer.
Though I was talking 50/50 water/coffee since all the people who tried it earlier in the post with straight coffee failed miserably. Coffee is too strong when it's 100% strength, you need to dillute it to 50% or less. Atleast that's my conclusion from reading 14 pages of posts.
- Silven
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Born: 10/31/83, which makes me a Scorpio.
1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.
What do you bring to the table?
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