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 sci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
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In a week or two I'm going to make some batches of agar with different dilutions of coffee in them and some with caffeine pills mixed in.
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Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)
Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)
PM for a trade.
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 amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 1 year, 4 months
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this guy boiled a bottle of beer and half the suggested amount of coffee. then he innoculated the beer with a multispore syringe. there was no apparent growth after 3 days.
a g2g jar of popcorn was soaked for 24hrs in water then pc'd. right after he did a g2g transfer. he then shot 2 syringes full of boiled uninnoculated beer coffee mix. after 3 days this jar is showing rhzimorphic growth. it looks fast too me, but i cant say for sure yet.
all of this guys experiments where stored @ 86f.
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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein
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TeknoJunkie
Pioneer
Registered: 05/27/03
Posts: 38
Loc: The Mountains
Last seen: 6 years, 8 months
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 Re: The Coffee Project [Re: Hippie3]
#2356920 - 02/19/04 07:48 PM (8 years, 3 months ago) |
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This sounds great! Perfect timing for me to read this to. I have a casing of mexicans I am just about to dunk....but now with a little something extra. I'll keep everyone updated.
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SoulJah2
Columbus of the inner globe
Registered: 05/17/02
Posts: 76
Last seen: 7 months, 28 days
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I tried dunking with diluted Tea on rye/verm cakes. Documented it here in mushroom cultivation forum. long story short all my cakes got lots of pins. None matured lots aborted then every single cake covered with brown slime.
search it up youll find my thread with more details if you wish
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That's not pretty cool.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
Last seen: 5 hours, 56 minutes
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 Re: The Coffee Project [Re: SoulJah2]
#2359208 - 02/20/04 11:03 AM (8 years, 3 months ago) |
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tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: The Coffee Project [Re: Anno]
#2359257 - 02/20/04 11:19 AM (8 years, 3 months ago) |
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Is'nt coffe on the acidic side of the ph scale ?
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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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mrjim202000
barefoot andfree
Registered: 02/20/04
Posts: 237
Last seen: 2 years, 10 months
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could you use spent coffee grounds as a casing layer ??
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dont tell lies, there will be less to remember
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 152,789
Loc: Pvt. Pubfag NutSuck 
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Quote:
tripndicular said:
Is'nt coffe on the acidic side of the ph scale ?
not enough so to prevent mycelial growth...
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: The Coffee Project [Re: SoulJah2]
#2359800 - 02/20/04 01:25 PM (8 years, 3 months ago) |
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Quote:
SoulJah2 said:
I tried dunking with diluted Tea on rye/verm cakes. Documented it here in mushroom cultivation forum. long story short all my cakes got lots of pins. None matured lots aborted then every single cake covered with brown slime.
search it up youll find my thread with more details if you wish
i read your thread,
but i see no good reason to think
you proved much of anything.
your cakes were old, already flushed.
you used tea, not coffee.
you don't mention rinsing after the dunk.
etc.
in other words, there are many potential
reasons for the cakes contam'ing.
it is interesting that you did start off with
many pins, a pity your cakes apparently died
before they could finish the job.
any time you dunk an older cake,
the risk is pretty high.
throw in a nutritious dunk
and the odds of failure go even higher.
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Admin @ mycotopia.net
Mycotopia
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 1 year, 4 months
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Re: The Coffee Project [Re: Hippie3]
#2359931 - 02/20/04 01:49 PM (8 years, 3 months ago) |
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you remind me of a non-rhyming dr. suess.
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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: The Coffee Project [Re: amyloid]
#2360288 - 02/20/04 03:17 PM (8 years, 3 months ago) |
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one fish,
two fish,
red fish,
blue fish.
ABC
LSD
gummi bears are chasing me.
one is green,
one is blue,
the yellow one
just took my shoe.
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Admin @ mycotopia.net
Mycotopia
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crime_think
Stranger
Registered: 02/08/04
Posts: 6
Last seen: 8 years, 7 days
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Re: The Coffee Project [Re: Hippie3]
#2362724 - 02/21/04 03:48 AM (8 years, 3 months ago) |
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i soaked millet in coffee and water (3 parts coffee, 5 parts water) and plan to add verm later as per the rhizo-tek in the grow log forum. I am still waiting for responses on my other thread to tell me whether or not the tyvek having gotten wet will lead to contam as i only have 2 syringes and will have to expend it all. Please post up here or on the other thread (search my name or whatever bc ive been pressure cooking for 20 hours and dont have the energy to post a single link) so i know if it is ok to inoculate. If i can, and all goes well, i will post my results on this thread. you might want to swing by the growlog forum and check out the rhizo-millet tek to see just what im talking about. thanks.
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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" the tyvek having gotten wet will lead to contam "
yes, that can occur
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Admin @ mycotopia.net
Mycotopia
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hevvy_psi
groover
Registered: 01/29/04
Posts: 10,446
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Re: The Coffee Project [Re: Hippie3]
#2363490 - 02/21/04 10:36 AM (8 years, 3 months ago) |
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everyone is talking about soaking their verm...will just using 50/50 water/coffee in my jars really work?
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egocentrism is bangin on the door
self-destructive selflessness
seeps out from the core
alone - eyes closed - an empty room
i'm curled on the floor
choose nothing, thus deciding
all the nothing i've in store.
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Jared
Stranger
Registered: 04/22/01
Posts: 8,783
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Re: The Coffee Project [Re: Jared]
#2363561 - 02/21/04 10:55 AM (8 years, 3 months ago) |
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Quote:
Jared said:
Quote:
Jared said:
I'm thinking of using straight coffee to moisten some vermiculite for use in a rez-effect casing.. I don't trust adding anything with nuts to the casing layer, and have no lime to buffer the ph, so I think this is a safe way to see how coffee affects my pinsets. I'm going to be doing two casings:
Control:
- Multispore GT
- 4 pints of substrate
- 1cm 50/50 casing
- 12"x10" cropping surface
- 25% addition of hydrated verm to substrate
Experiment:
- Multispore GT
- 4 pints of substrate
- 1cm 50/50 casing
- 12"x10" cropping surface
- 25% addition of coffee hydrated verm to substrate
If there is anything about this you think I should change, speak up.
When I did the two casings, I first combined all of the substrate in one container, and crumbled it into rice sized peices, and mixed thoroughly to try and minimize the inconsistencies caused by multispore inoc.
Feb 15th: the coffee casing is saturated with pins, with roughly twice as many as the regular casing. They are about a day ahead of the non-coffee pins. So far, coffee is lookin very nice  I'm just waiting to see if the abort rate is high =X.. if not, im casing with coffeesized verm all the time.
Total dry results:
Coffee: 1.55 ounces
Control: 1.1 ounces
Keep in mind this was multispore.. but so far.. I'm impressed with coffee.
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: The Coffee Project [Re: Jared]
#2364076 - 02/21/04 01:01 PM (8 years, 3 months ago) |
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that's a pretty significant difference in yield,
i'm impressed with coffee, too.
thx for the update.
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Admin @ mycotopia.net
Mycotopia
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: The Coffee Project [Re: Hippie3]
#2364160 - 02/21/04 01:40 PM (8 years, 3 months ago) |
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some agar w/coffee, w/control.
Quote:
myco domesticus (Mycophil)
Senior Member
Username: Mycophil
Post Number: 823
Registered: 10-2002
Posted on Saturday, February 21, 2004 - 05:50 pm:
--------------------------------------------------------------------------------
these are the agar results thus far...the speed of germination doesn't seem to be altered by the coffee but once germinated it looks like the coffeeagar has more enthusiastic, abundant growth
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Admin @ mycotopia.net
Mycotopia
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amyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 1 year, 4 months
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Re: The Coffee Project [Re: amyloid]
#2367312 - 02/22/04 08:19 AM (8 years, 3 months ago) |
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just an update on my experiments.
the coffee + beer liquid, which was boiled prior to being cooled and grain innoculated is showing what i think is mycellium, ive havent seen any good pictures of mycellium in liquid so i cant really tell. there are clumps of stuff floating around and settling on the bottom, they look like the clumps of myco that are in old syringes to me, but the beer/coffee is hard to see through.
the jar of popcorn that was pc'd with just water, then had a squirt or 2 of beer+coffee, was innoculated using g2g with colonized popcorn, which had very thick and slow growing (tomentose) mycellium. 5 days later it is showing significantly faster growing mycellium which is all rhizomorphic (maybe linear too?.)
obviously not very conclusive but its still results of coffee experiments.
i can take pictures if u want to see the growth.
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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein
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BLuEFroG
Friend of Many
Registered: 02/13/04
Posts: 425
Loc: Eastern Coast
Last seen: 6 years, 3 months
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Re: The Coffee Project [Re: amyloid]
#2367733 - 02/22/04 11:18 AM (8 years, 3 months ago) |
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Just to throw this in...
1) Hope you got all the alch. out of the beer
2) what would the beer do for the mycellium? All of the sugar is used up by the yeast to produce alchohol and all your doing is boiling off the product...the only thing I think would really do anything is the caffene in the beer...
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: The Coffee Project [Re: BLuEFroG]
#2368219 - 02/22/04 01:13 PM (8 years, 3 months ago) |
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the addition of the beer does make the interpretation of any results more difficult.
better to omit extra variables
while testing the coffee or caffeine itself.
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Admin @ mycotopia.net
Mycotopia
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