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Invisibleazur
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Re: taping holes in monotubs/casing at spawning [Re: insanemike]
    #22452855 - 10/30/15 09:32 AM (8 years, 4 months ago)

Well damn mike, how is one to even respond to that?
Your data is proving more useful every day. Thank you for your diligent work. Many people will reap the rewards for a long time.

I just spawned 7 APE tubs. Don't have casing material, so i covered the holes and will case tonight and put poly in the holes. I don't think "choking" it for 10 hours will hurt it.


--------------------


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FALL IN LOVE WITH LC
FOTTSE!!!
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Invisiblepaperbackwriter
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Re: taping holes in monotubs/casing at spawning [Re: azur]
    #22452876 - 10/30/15 09:41 AM (8 years, 4 months ago)

Great post mike!

My non-scientific translation.

Mycelium always likes to breath.  When we're colonizing the tubs there's less mycelium (up to a point) so they need less air.  When the mycelium is fruiting it's doing more work and therefore needs more air.  But it always needs some air.  How much air is based on how much it's exerting itself and how big the mycelial network is.

Is that about right?

I think colonizing with low air exchange may be a holdover from people thinking they had to keep contams out during colonization.  In other words, it's superstition based on not understanding that first flush contams are from bad spawn or bad pasteurization.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley

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Invisibleinsanemike

Registered: 02/23/14
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Re: taping holes in monotubs/casing at spawning [Re: azur]
    #22452887 - 10/30/15 09:45 AM (8 years, 4 months ago)

Nah. There is plenty of oxygen in them tubs. I would confidently say that even going as far as up to 24 hours of limited gas exchange wouldn't effect the system. Especially not with in the first 24 hours of spawn run. :thumbup:

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Invisibleinsanemike

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Re: taping holes in monotubs/casing at spawning [Re: paperbackwriter]
    #22452894 - 10/30/15 09:46 AM (8 years, 4 months ago)

Quote:

paperbackwriter said:
Great post mike!

My non-scientific translation.

Mycelium always likes to breath.  When we're colonizing the tubs there's less mycelium (up to a point) so they need less air.  When the mycelium is fruiting it's doing more work and therefore needs more air.  But it always needs some air.  How much air is based on how much it's exerting itself and how big the mycelial network is.

Is that about right?

I think colonizing with low air exchange may be a holdover from people thinking they had to keep contams out during colonization.  In other words, it's superstition based on not understanding that first flush contams are from bad spawn or bad pasteurization.




:thatsayes:

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Invisiblepaperbackwriter
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Re: taping holes in monotubs/casing at spawning [Re: insanemike]
    #22452947 - 10/30/15 10:02 AM (8 years, 4 months ago)

Makes me wonder if the fast pinning azur is seeing with PE (and the slow pinning that's typical) is because the genetics use more oxygen.

Here's an old thread showing how PE fruit outdoors.  Took three weeks for his bed to colonize and fruit.  Five weeks from inoculation until first flush harvest.

Quote:

The total time from grain spawn to fruit was five weeks. One week for the grain to colonize (grain to grain form agar inoculated grain master), another week or so for dung crates to colonize and then three weeks for beds to colonize and fruit. The bed is now on its second flush. The main bed featured here only took 4 pint jars to create. 4 pints = 8 milk crates (4" deep) = +/- 12 sq. feet of bed. Total dry pull was 25 ozs from first flush.





http://www.shroomery.org/forums/showflat.php/Number/14847917/fpart/1/vc/1


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley

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Invisiblepaperbackwriter
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Re: taping holes in monotubs/casing at spawning [Re: paperbackwriter]
    #22453191 - 10/30/15 11:21 AM (8 years, 4 months ago)

Tub one, my straight into fruiting tub, now has a number of pins.  I see a little cluster forming I'm going to try to get a clone from.

Tub three, the one I let consolidate until it pinned, is more knotted than either tub one or two, and has a pin as well as quite a few primordia.  So it went into fruiting today (13 days after spawning).

These are all MS so some variety is to be expected.  I'll probably take a clone from both tub one and tub three.  Tub two seems to be the dud so far which was the one that was colonized more typically.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley

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Invisibleeatyualive
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Re: taping holes in monotubs/casing at spawning [Re: paperbackwriter]
    #22453300 - 10/30/15 11:54 AM (8 years, 4 months ago)

Quote:

paperbackwriter said:
Great post mike!

My non-scientific translation.

Mycelium always likes to breath.  When we're colonizing the tubs there's less mycelium (up to a point) so they need less air.  When the mycelium is fruiting it's doing more work and therefore needs more air.  But it always needs some air.  How much air is based on how much it's exerting itself and how big the mycelial network is.

Is that about right?

I think colonizing with low air exchange may be a holdover from people thinking they had to keep contams out during colonization.  In other words, it's superstition based on not understanding that first flush contams are from bad spawn or bad pasteurization.



no, people used to colonize tubs this way long ago. when i was doing it around 2001, it was definitely the norm. somewhere along the line, someone decided it was necessary. which is not the case.


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Invisiblefilthyknees
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Re: taping holes in monotubs/casing at spawning [Re: eatyualive]
    #22456864 - 10/31/15 08:43 AM (8 years, 4 months ago)

Good on ya mike.

Some how related i found was a radio show I heard the other day. About arachnids. They breathe by air moving over their bodies, if they move too fast and run out of breath they have to stand still to catch up. I imagine it's relative to the mycelium as its getting into a sprint (day2-4) it would react much the same, having to slow down to breathe. Loving this thread. Changing the way it's done. :thumbup:


Good luck with your tests!

Ps did you know arachnids shed their respitory and digestive systems? :eek:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
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Invisibleinsanemike

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Re: taping holes in monotubs/casing at spawning [Re: filthyknees]
    #22456942 - 10/31/15 09:21 AM (8 years, 4 months ago)

Thanks for that bit of info. I love learning things like that. :rockon:

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OfflineDeTwizzle
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Re: taping holes in monotubs/casing at spawning [Re: insanemike]
    #22457435 - 10/31/15 11:53 AM (8 years, 4 months ago)

A p. subfusca(Highland Kandy Ornamental)of mine!

Happy Halloween!


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OfflineDrCrumbs
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Re: taping holes in monotubs/casing at spawning [Re: DeTwizzle]
    #22461387 - 11/01/15 10:08 AM (8 years, 4 months ago)

I had always thought that the taping had to do with protecting the spawn/substrate from contams. Didn't realize there was some kind of CO2 thought going on to induce pinning. Which clearly doesnt work anyway at least not for me since its usually about 2 weeks before pins after removing the taped holes (It could have been other factors as well).

Since coir is now known to be so contam resistant it makes total sense. Well I guess also the poly should work well as a filter anyway so more nutritious substrates would be fine right (like straw)?

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Invisibleeatyualive
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Re: taping holes in monotubs/casing at spawning [Re: DrCrumbs]
    #22461450 - 11/01/15 10:22 AM (8 years, 4 months ago)

Yes. I'm using dung in my tubs and there are no issues either.


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Edited by eatyualive (11/01/15 10:31 AM)

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InvisiblePastywhyteMDiscord
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Re: taping holes in monotubs/casing at spawning [Re: eatyualive]
    #22461475 - 11/01/15 10:27 AM (8 years, 4 months ago)

Well the tub I spawned with the bottom holes stuffed had the myc blow right through the top layer. Gonna patch er up and dial the FAE up for fruiting today. Looks pretty healthy.

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Invisibleazur
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Re: taping holes in monotubs/casing at spawning [Re: Pastywhyte]
    #22461569 - 11/01/15 10:46 AM (8 years, 4 months ago)

All my tubs get poly from the get now.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisibleeatyualive
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Re: taping holes in monotubs/casing at spawning [Re: azur]
    #22461647 - 11/01/15 11:06 AM (8 years, 4 months ago)

I second that notion and all my tubs will be cased at spawning as well.


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Invisibleazur
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Re: taping holes in monotubs/casing at spawning [Re: eatyualive]
    #22461657 - 11/01/15 11:09 AM (8 years, 4 months ago)

Yes. Any tub that requires it (pe, ape, peu) will be cased at spawning.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisiblewowimflabbergasted
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Re: taping holes in monotubs/casing at spawning [Re: azur]
    #22461831 - 11/01/15 11:45 AM (8 years, 4 months ago)

So poly and top layer at spawning FTW. Case again after 100% or no?


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Invisibleeatyualive
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Re: taping holes in monotubs/casing at spawning [Re: wowimflabbergasted]
    #22461842 - 11/01/15 11:47 AM (8 years, 4 months ago)

Nope.


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Invisiblewowimflabbergasted
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Re: taping holes in monotubs/casing at spawning [Re: eatyualive]
    #22461850 - 11/01/15 11:48 AM (8 years, 4 months ago)

well fuckin a


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InvisiblePastywhyteMDiscord
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Re: taping holes in monotubs/casing at spawning [Re: eatyualive]
    #22461869 - 11/01/15 11:51 AM (8 years, 4 months ago)

Quote:

wowimflabbergasted said:
So poly and top layer at spawning FTW. Case again after 100% or no?



Quote:

eatyualive said:
Nope.




Hey eat, how thick do ya make yer top layers in these scenarios? Mine is all eaten up, so I was gonna patch heavy. Was about to do it just now. This culture really does prefer a casing. What is your opinion?

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Shop: North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Substrate Mix   Myyco.com APE Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


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