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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Tomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
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sterilizing straw?
#2213382 - 01/01/04 03:05 PM (20 years, 2 months ago) |
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what would be the problem with sterilizing straw in the microwave?
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tchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 9 years, 6 months
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either too little finished product or burning down the house!
straw can be pc'd if you keep it off the bottom of the pc, but... usually people just pasturize straw. it is actually rather difficult to sterilize, hence the problem with burning.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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If you sterilize straw, it will likely contaminate before you can get it colonized. Only pasteurize straw for best results.
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Tomandjerry58
Stranger
Registered: 01/27/03
Posts: 5,212
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makes me feel good to have the great rabbit answer my question.thanks. ive only gotton the straw to work for me twice out of four times.with pasturization. i was thinking that it might be possible to inccorporate a little straw with some verm. to up the potency of my buddies. im giving it a try 2 quarts colonized birdseed 1 hand full of straw and 1 quart of verm . will see how it goes.thanks for the reply
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SixCee
keep rolling
Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
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You could up the volume of your substrate, but not the potency of the finished fruits. Vermiculite has no nutritional value so adding it to straw wouldn't supply it with more nutrients. It would hold more water however which is a good thing.
-------------------- ~~~~~~~~~~~~~~~~~~~~~~~~ -->The above statements may or not be true. ->Quote of the Moment : "Yea. All bitches are whores who love sex." -Cubie ----> PMs checked daily.
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Ayahuashka
Stranger
Registered: 07/08/17
Posts: 2
Last seen: 6 years, 8 months
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Hey!
I just joined here serching info and found your post.
I can talk about my experience with the microwave sterilization, cause I'm testing things with it right now.
For the sterilization of little amounts of substrate, for me, worked better than using a pressure cooker. Microowave wastes lot of electricity so, for a big amounts (and thinking that the capacity of a micro is about 25-30 l), its better to use an autoclave, big pressure cooker or else.
I using the micro to make easy the process with grain jars or little bags of spawn. I ever cook the grain/straw/hardwood chips first before sterilizate, so thats the first chance to eliminate the contaminants.
With grain is better to put them on water before do anything, for like 12h to let spores of contaminants activate and become suceptible to the heating.
Next ill try to give a recipe of how I make this:
- Soak grain 12h - Cook grain about 20-40 min (when a bit of foam forms on the surface and water start to be brown) - Let the grain dry on a clean wringer posted on a clean cooking pot and covered with a kitchen paper for about 2-4h (when u see grain surface not excessively wet) on a clean place (hot dry). - Pre-sterilize the surface of your clean and asceptic containers or bags (with a bit of 96º alchool) before adding the grain. - Add the grain having in mind that your hands or the tool that is going to touch the substrate to fill the pots, must be clean and sterile, so u will need to use 96º alchool several times during the proces. * When u fill the containers, have in mind too that u only have to open them the minimal time for introducing the substrate, so dont expose the inside to posible contaminants. - If u work with bags, let a bit open the zip, cause they will inflate inside the micro, and dont close at all the pots or jars, to let pressure escape. When u have the pots/bags filled, u need to use a 2nd bag or container to allocate them inside, and then the 2nd container inside the micro. That 2nd container (that can be another plastic bag, like a microowave or frozen bags or "potato microwave cooking bags", like the one I use, bought at china bazar xD). - Put a bit of destilated water inside this bag, -having cure that the pots or spawn bags inside dont get wet- to generete steam during the cooking. Also dont close at all the 2nd container. - Now its all prepared to run the microowave at minium 800 W or medium-high power. I recommend to dont run the micro at full pow. *If the micro have a rotating plate, have in mind that if the objects inside block the rotation, u can burn the enginee that rotates the plate. Anyway, me really dont care too much for that when I do this process, but i have to mention it
Generally I put a 15 min cicle to complete the sterilization, but with 10 minutes probablly its enought.
The rest of the process is the same that u sterlize with any other methods, u will have to inoculate on a clean enviroment with asceptic conditions, etc.
I maked this process many times and works fine for me. I used the same process with little bags of supermarchet filled with straw and 0 contaminantions :P Micellium grow fine on it (Pleurotus).
Edited by Ayahuashka (07/08/17 10:54 AM)
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vikingleif
Lazy Stoner
Registered: 08/11/16
Posts: 188
Last seen: 4 years, 4 months
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then if you just started searching then plz learn how to use the function to not ressurect posts from over 10 years ago :O
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Pipefitter537
You're Not Yelping
Registered: 11/25/16
Posts: 715
Loc: SodoSopa
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-------------------- "You should never underestimate the predictability of stupidity"-Bullet Tooth Tony
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Ayahuashka
Stranger
Registered: 07/08/17
Posts: 2
Last seen: 6 years, 8 months
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I dont undrstand the point of what u say. ¿Whats wrong with following this post?
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vikingleif
Lazy Stoner
Registered: 08/11/16
Posts: 188
Last seen: 4 years, 4 months
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there is a search function on this forum by clicking "search our forum" then you can specify your search so that you dont get older posts than last 3-4 years instead going full time machine and getting really old awnsers.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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This thread has been closed.
Reason: 13 year old thread
Use the search engine effectively https://www.shroomery.org/forums/showflat.php/Number/24270830#24270830
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