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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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PsiloSeaBean
Stranger
Registered: 09/12/13
Posts: 52
Last seen: 5 years, 9 months
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Nope, that's not the reason, the cake is nice, no worry. The reason, why it looks like sludge is, because I pushed it after filling it, so the water and the brf build sludge at the wall of the glass, so no sludge at all and definitly no mold. The mycel grows very nice, the question is wheater there is bacteria.
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amnesia
Stranger
Registered: 02/12/15
Posts: 4
Last seen: 9 years, 22 days
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If i dont have any acces liquid spore or print is there anyway to harvest in straight from the fruit?
-------------------- My girlfriend cook very well
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Re: Pf tek done right TEK [Re: amnesia]
#21266474 - 02/12/15 09:36 PM (9 years, 1 month ago) |
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Yes,
Take prints from your flush or get into agar, rip your shroom in half and take an inner tissue sample.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Zound
Stranger
Registered: 02/19/15
Posts: 19
Loc: SE QLD
Last seen: 8 years, 6 months
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Re: Pf tek done right TEK *DELETED* [Re: taGyo]
#21867247 - 06/28/15 05:04 AM (8 years, 8 months ago) |
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Post deleted by ZoundReason for deletion: none
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GreenRabbit
Plutonium Pollinator
Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 5 months
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Re: Pf tek done right TEK [Re: Zound]
#21867780 - 06/28/15 09:40 AM (8 years, 8 months ago) |
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PF tek done right? You are using a pressure cooker. Your first steps should have been:
1. Agar 2. Grain 3. Bulk
The only reason I ran with PF tek for 2 years is because I didn't have a pressure cooker. Please don't advise people who have proper equipment to use such a low yielding, time consuming method with a high contamination rate.
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Zound
Stranger
Registered: 02/19/15
Posts: 19
Loc: SE QLD
Last seen: 8 years, 6 months
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I have a pressure cooker yes.
Could you link me to the agar tek that you recommend?
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GreenRabbit
Plutonium Pollinator
Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 5 months
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Re: Pf tek done right TEK [Re: Zound]
#21869849 - 06/28/15 06:21 PM (8 years, 8 months ago) |
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)
Registered: 11/12/11
Posts: 26,370
Loc: Boston
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IMO the easiest and fastest way for spawn, is to use the PF tek. (when using MS)
what I do, and will probably do my whole life, is do the PF tek up until I have myc in the jars. Then I crumble the cakes and spawn to bulk. Each jar makes 1 tray. Each tray makes an average 10-15 dry grams.
I use "humidity domes" for the FC. Its literally TOO easy. Its like taking candy from a baby
so really, all I spend money on after my first go with the method, is spore syringes, and bulk substrate. I could even just make my own bulk sub but im lazy.
I already have the jars, the PC, the FC's...everything.
im sure theres better ways to grow, but this way is just my personal favorite because of the simplicity mixed with the time I spend, the success rate...etc..
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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GreenRabbit
Plutonium Pollinator
Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 5 months
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PF to bulk has been my favorite method so far but it is too time consuming. I hate waiting for jars to colonize, and waiting 1-3 weeks just to see if spores germinate is a pain. Even after that, they still have a month to contaminate.
With agar, everything is sped up, and you never have to buy syringes again.
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Bill_Oreilly
ANIMALS (the RAINBOW SERPENT)
Registered: 11/12/11
Posts: 26,370
Loc: Boston
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Quote:
GreenRabbit said: PF to bulk has been my favorite method so far but it is too time consuming. I hate waiting for jars to colonize, and waiting 1-3 weeks just to see if spores germinate is a pain. Even after that, they still have a month to contaminate.
With agar, everything is sped up, and you never have to buy syringes again.
of course agar is the best. im just talking about MS grows.
PF tek to bulk is the shittt
and really, I think its better to wait a month for jars to colonize than to wait like 2 months for quart jars to colonize. I know you get like triple the amount but still...just noc up more jars
-------------------- Something there is mysteriously formed, Existing before Heaven and Earth, Silent, still, standing alone, unchanging, All-pervading, unfailing, I do not know its name; I call it tao. If forced to give it a name, I call it Great (ta). Being great, it flows out; Flowing out means far-reaching; Being far-reaching, it is said to return. It's just a shot away..
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Zound
Stranger
Registered: 02/19/15
Posts: 19
Loc: SE QLD
Last seen: 8 years, 6 months
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A few of days later and something is happening in the jars at all the spots where I inoculated. But I'm not sure if its cobweb mold? What do you think?
Edited by Zound (07/01/15 05:11 AM)
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TurtleUp
Danger
Registered: 07/03/15
Posts: 29
Last seen: 7 years, 3 days
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Re: Pf tek done right [Re: Zound]
#21895345 - 07/04/15 01:44 AM (8 years, 8 months ago) |
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Lots of good info!
--------------------
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Divinori
Stranger
Registered: 01/26/14
Posts: 497
Last seen: 3 years, 8 months
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Re: Pf tek done right [Re: TurtleUp]
#21899991 - 07/05/15 07:59 AM (8 years, 8 months ago) |
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Is there something you can do without the 6500K light or do you think it's a must?
-------------------- “Life lived in the absence of the psychedelic experience that primordial shamanism is based on is life trivialized, life denied, life enslaved to the ego.”
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Mr. Alien
I will abduct andprobe your anus
Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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Re: Pf tek done right [Re: Divinori]
#21901429 - 07/05/15 02:54 PM (8 years, 8 months ago) |
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Quote:
Divinori said: Is there something you can do without the 6500K light or do you think it's a must?
Is not a must. Just use your fruiting chamber close a window, avoid direct sunlight.
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homerj
dreamer
Registered: 11/25/15
Posts: 11
Last seen: 7 years, 2 months
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Re: Pf tek done right [Re: Mr. Alien]
#22666733 - 12/17/15 08:30 AM (8 years, 3 months ago) |
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yep, just put it in a room that gets enough sunlight to read by and you should be good.
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Boogens
Boogens
Registered: 06/18/15
Posts: 96
Loc: Canada
Last seen: 3 years, 18 days
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Re: Pf tek done right TEK [Re: Shea25]
#22970706 - 03/03/16 07:00 PM (8 years, 26 days ago) |
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Is it ok to use 91% ISO?
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Munchauzen
Registered: 06/22/11
Posts: 14,343
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Re: Pf tek done right TEK [Re: Boogens]
#22970719 - 03/03/16 07:04 PM (8 years, 26 days ago) |
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just add some water to make 70% bro. its been scientifically proven 70% is a better sanitizer than other percentages
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Pf tek done right TEK [Re: Munchauzen]
#22970862 - 03/03/16 07:39 PM (8 years, 26 days ago) |
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91% iso is inferior to 70% use 70% next time. it denatures proteins water is miscable with alcohol and helps it penetrate cell walls.
C1V1=C2V2 is the concentration calculation you can dilute it to make 70% with distilled water
(0.70)(650mL)=(.91)(500mL)
add 150ml of water to 500ml of 91% yields you 650mL of 70%.
Quote:
70% is far superior as a sanitizer than 91% you can turn 91% into 70% with distilled water
(70%)(130ml)=(91%)(100ml) so to 100ml of 91% add 30ml H2O to achieve 70%
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Mycolamb
ShroomPool
Registered: 07/01/16
Posts: 6
Last seen: 7 years, 4 months
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Re: Pf tek done right TEK [Re: Shea25]
#23432728 - 07/11/16 05:55 PM (7 years, 8 months ago) |
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Quote:
Shea25 said: PF tek Things needed
1/2 pint wide mouth canning jars Pot/Pressure cooker Vermiculite Perlite Brown rice/Brow Rice Flour Tin foil 70% Iso alcohol Plastic Tote 6500K florescent bulb 1/4 inch drill bit/Drill Hammer/Nail Mixing bowl and spoon Gypsum Spore syringe Latex gloves Dust mask Lighter
Preparing the jar
Take the lids and using a nail and hammer or a 1/8 drill bit, drill 4 holes near the rim of the lids nicely spaced out. Depending what way you use you lids i use mine rubber side down just punch the holes so they don’t leave burrs pointing towards you.
The basic recipe for 5 PF jars is this 2 parts vermiculite 1 part water and 1 part BRF (additionally you can add 1 tablespoon of gypsum for 5 jars)
In a bowl add the 2 cups of vermiculite (and gypsum if you use it) now add the 1 cup of water, mix really well. Now add the 1 cup of Brown rice flour (you can make your own by grinding brow rice in a coffee grinder) Mix this really really well. Now load this into the 5 canning jar equally, making sure not to compress the mixture in the jars you want it nice and airy. You will be leaving a 1/2 inch space at the tops of the jars for the dry vermiculite layer.
Take a paper towel and wipe the 1/2inch space you left at the tops of the jars making sure to wipe away any BRF mixture or moisture away. Now top off the jars with dry vermiculite, screw on the lids and cover the tops the lids with tin foil. The tin foil is there to keep any moisture from entering the jars while there sterilizing.
Sterilizing
Now you want to take you PC or (pot with a tight fitting lid) and fill the bottom of it with jar lid rings and tin foil this is to keep the bottom of your jars off the bottom of the PC/Pot so they don’t burn Some PC’s come with trays to keep the jar already off the bottom use that if you can. Once that is done put the jars into the PC/pot and fill it with cold water just almost to the bottom of the jars. Now put on the lid of the PC/Pot and put the heat on High. Once the rocker on top of the PC starts to rock turn the heat right down to just keep it to a slight rock and start your 60 minute count down, If you’re using a pot once it come to a boil turn it down to a slight simmer and start your 90 minute count down. After the 60(PC) or 90(Pot) minutes are up turn off the heat and walk away let them cool down over night or for 8 hours.
Inoculation
Once the jars are cooled down put them in a draft free room along with the 70% Iso,Lighter,spore syringe . Make sure the rooms windows are shut and AC or heater turned off. Wash your hands and arms really good. Now put of the gloves and mask Take the foil lids off the jars you won’t need them anymore. Now rub your hand down with the 70% ISO wait a few seconds for it to evaporate. Now shake the syringe really well, using the lighter heat the needle of the syringe till its red hot now proceed to inoculate the jars using a 1/4 cc of solution per hole inoculating towards the glass. That’s 1 cc per jar. After every jar heat the needle back till its red hot and repeat.
Incubation
Now put the jars away in a closet of shelf at room temp they will start to colonize in a few days. Keep an eye on them every 4 days or so. Once they appear to be 100% colonized let them sit for another week to consolidate.
Birthing and dunk
Once the jars are finished their 1 week consolidation it’s ready to get them out of the jars. Open the jars get rid of the dry vermiculite layer now slap the jars (upside down) onto a wood cutting board or counter top, trying to coax the cakes out of the jars. Once out rinse them off and submerge in cool water for 24 hours making sure they stay submerged. After 24 hours rinse them again and now roll the cake in dry vermiculite making sure to cover the cake well tops and bottoms. I find busting up some of the verm for the dry roll up alittle finer helps.
Fruiting
Build one of these http://www.mushroomvideos.com/Terrarium-Tek
I personally use a PMP
Mist your cakes 3-4 times a day and fan right after, fan 5-6 times a day. Water evaporating off your cakes is a major pinning trigger. Give them a 12/12 light schedule using a 6500K Florescent bulb (daylight or cool blue). Once they have pinned and grown and all picked, dunk them for 24 hours again this time no dry roll in vermiculite and put back in the Shotgun Fruiting Chamber.
Good Luck
Tips
Gypsum really helps the development of the fruit bodies I recommend using it Room temp 70-75 is perfect for incubation and fruiting. There’s no need to keep the dunking cakes in the fridge Mist and Fan Mist and Fan Use short wide mouth 1/2 pint jars Dont cover the lids after inoculation with anything example, Foil/tape Give them love
Results in this Golden Teacher
This was 2 cakes first flush, came just under 19 grams dry. Oak Ridge
Heres another set of cakes
Results may vary
Here is a How to on sterilizing a syringe to make up a spore syringe
How to sterilize a syringe with just a pot water and syringe
Thing needed.
A pot Empty Syringe Water(tap can work)
Fill the pot with cold water. turn the burner on High and wait for the water to come to a full boil. now start a 10 minute countdown and keep the water at a full boil.
After 10 minutes take the empty syringe and suck back the boiling water and let it sit in the syringe for 1 minute then expel the water in a sink(not back in the pot)
Repeat 4 more times on the last time keep the water in the syringe cap it and let it cool down.
Hope this helps.
x4
Notes: Use a Q-tip and 70% alcohol to clean the inside of the cap I have found even after months and months they will stay sterile No need to store in the fridge Never try to cool them down fast in the fridge
-------------------- Send me a PM and chat for a while
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 10,017
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Re: Pf tek done right TEK [Re: Mycolamb] 1
#23433294 - 07/11/16 09:11 PM (7 years, 8 months ago) |
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I hate when people quote entire posts for no reason.
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