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Syntheticwords
Victus
Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
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The Road to 7 mono tubs
#21736944 - 05/29/15 01:53 PM (8 years, 9 months ago) |
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Greetings Humans,
I have recently undertaken a project for an assosciate of mine I will use my powers for good! Never evil...
So here we begin. I will work and update this post through my ventures
I began with 3 syringes
Cambodian Amazon Creepers
Using these strains I created 12 jars of liquid culture
With these jars I will make 10 x 10ml syringes And I plan to test out the LC tonight to see if it's ready for bulk
I plan to use all the syringes to knock up 42 Quart jars of WBS
That's a whopping 7 mono tubs expected for this project
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PinPornProducer
Buy the ticket, take the ride
Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 2 months
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Good luck man, just a head up though, you may want to test those lcs before going balls out with them. They can be very deceiving and bite you right in the ass very quick.
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Syntheticwords
Victus
Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
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I plan to test each jar with a brf cake tonight
They are currently in the PC cooling down
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PinPornProducer
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Registered: 08/23/14
Posts: 9,981
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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If you have spores left, you might as well just knock up a bunch of jars with spores. It only takes a tiny amount of spores per jar, maybe 1/10 of a ml. By the time those BRF cakes are done you would have plenty of spawn.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Syntheticwords
Victus
Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
#21737005 - 05/29/15 02:11 PM (8 years, 9 months ago) |
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Well the brf cakes are just my testers. No need to wait until they are finished
If I see good healthy spread of mycelium, then I will use the LC to knock up WBS qt jars
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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No, you still need to wait for them to finish. Mold and bacteria can show up at any time.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PinPornProducer
Buy the ticket, take the ride
Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 2 months
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
#21737025 - 05/29/15 02:14 PM (8 years, 9 months ago) |
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and fruit them, mold doesn't show up sometimes until it's introduced to fresh air (fruiting conditions)
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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I would fruit them anyway, cause why waste spawn, but you don't really need to go that far. If they colonize clean then it should work.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Syntheticwords
Victus
Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
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So before I can determine if my LC is healthy I have to wait until cakes are done? Under my time parameters It may not be viable to wait to fruit the cakes. The real treasure is in the bulk.
Your saying mold and bacteria are harboring inside the mycelium ? Fruiting conditions won't be introduced until ....the substrate has consolidated?
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PinPornProducer
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It's the same as bulk, grains look healthy and clean but once spawned and fruited, if the spawn was bad, 3-4 days later it tams out. That's why we use agar, contams can't hide. Trich mycelium hides very well within mushroom myc. If you don't want to wait until fruits then test it on agar, you will know within a few days to a week if it's clean or not
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,830
Loc: Canada
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FYI its best to test LC on agar. You can see right away on the 2D media if its clean or not. Its also better to inoculate LC with agar wedges anyway so the two go hand in hand IMO.
Given that its nearly impossible to have a 100% aseptic LC you need to use it as soon as you can after colonization. Agar gives an edge there for sure.
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Syntheticwords
Victus
Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
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Re: The Road to 7 mono tubs [Re: Pastywhyte]
#21737137 - 05/29/15 02:42 PM (8 years, 9 months ago) |
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Currently the LC is on its 10th day
And agar would probably take a while to ship here As a future reference those helps tremendously
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,830
Loc: Canada
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I buy agar in an asian super market for cheap. Add your own nutes and its done. It can also be found in health food stores.
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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Quote:
Syntheticwords said: So before I can determine if my LC is healthy I have to wait until cakes are done? Under my time parameters It may not be viable to wait to fruit the cakes. The real treasure is in the bulk.
Then wait the proper amount of time. Rushing things leads to failure, especially when you're new enough to have to ask these questions.
Trust me, putting shit off 2 weeks is much better than throwing away 7 tubs' worth of shit. Learn from my mistake, not yours.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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PhosCap
Gratuitous Heavenly Grace
Registered: 06/09/10
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
#21739203 - 05/29/15 11:13 PM (8 years, 9 months ago) |
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I remember reading last year or so Frank went balls deep in LC's and then ultimately lost many tubs due to that unknown factor of contam. Then he bypassed and went straight to agar. I personally never done LC because of what i read. Agar seems the best, easiest route.
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36fuckin5
Alchemycologist
Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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Re: The Road to 7 mono tubs [Re: PhosCap]
#21739218 - 05/29/15 11:17 PM (8 years, 9 months ago) |
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I've done lots and lots of LC. There's a reason I quit. If it works you have a fuckload of inoculant ready at once. A couple quarts of LC does a LOT of jars or bags. If it doesn't work, or you happen to contaminate it during a transfer, you lose just as much. Not worth it. Methods need to work 100% of the time for 100% of people who take time to do it properly.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Syntheticwords
Victus
Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
#21752126 - 06/02/15 10:33 AM (8 years, 9 months ago) |
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UPDATE:
Brf cakes are showing signs of healthy growth If I hadn't posted on this forum I would probably Feel extremely confident
However. Many of you make me question my work I will heed your warnings and order a pack of petri agar dishes
Pics coming soon
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slapphappypill
Enthusiast!
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
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next stop on that road, 100 monotubs :-P
good luck and keep posting pics/questions as needed!
-------------------- We think we have freedom, but we're all just mice in a maze.... FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly! I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably! ~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~ ~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~ ~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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BurberrySpores
72 zones
Registered: 08/05/14
Posts: 1,013
Last seen: 6 hours, 12 minutes
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Lol. I'm working on 20 right now
-------------------- I could go crazy... and no one would notice
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