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OfflineSyntheticwords
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Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
The Road to 7 mono tubs
    #21736944 - 05/29/15 01:53 PM (8 years, 9 months ago)

Greetings Humans,

I have recently undertaken a project for an assosciate of mine
I will use my powers for good! Never evil...

So here we begin. I will work and update this post through my ventures

I began with 3 syringes

Cambodian
Amazon
Creepers

Using these strains I created
12 jars of liquid culture



With these jars I will make 10 x 10ml syringes
And I plan to test out the LC tonight to see if it's ready for bulk


I plan to use all the syringes to knock up
42 Quart jars of WBS

That's a whopping 7 mono tubs expected for this project

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OfflinePinPornProducer
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Registered: 08/23/14
Posts: 9,981
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21736964 - 05/29/15 02:00 PM (8 years, 9 months ago)

Good luck man, just a head up though, you may want to test those lcs before going balls out with them. They can be very deceiving and bite you right in the ass very quick.

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OfflineSyntheticwords
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Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21736979 - 05/29/15 02:03 PM (8 years, 9 months ago)

I plan to test each jar with a brf cake tonight

They are currently in the PC cooling down

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OfflinePinPornProducer
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21736982 - 05/29/15 02:04 PM (8 years, 9 months ago)

:super:

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21736990 - 05/29/15 02:07 PM (8 years, 9 months ago)

If you have spores left, you might as well just knock up a bunch of jars with spores. It only takes a tiny amount of spores per jar, maybe 1/10 of a ml. By the time those BRF cakes are done you would have plenty of spawn.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflineSyntheticwords
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Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21737005 - 05/29/15 02:11 PM (8 years, 9 months ago)

Well the brf cakes are just my testers. No need to wait until they are finished

If I see good healthy spread of mycelium, then I will use the LC to knock up
WBS qt jars

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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21737014 - 05/29/15 02:12 PM (8 years, 9 months ago)

No, you still need to wait for them to finish. Mold and bacteria can show up at any time.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflinePinPornProducer
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Registered: 08/23/14
Posts: 9,981
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Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21737025 - 05/29/15 02:14 PM (8 years, 9 months ago)

:whathesaid: and fruit them, mold doesn't show up sometimes until it's introduced to fresh air (fruiting conditions)

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21737038 - 05/29/15 02:16 PM (8 years, 9 months ago)

I would fruit them anyway, cause why waste spawn, but you don't really need to go that far. If they colonize clean then it should work.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflineSyntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
Re: The Road to 7 mono tubs [Re: PinPornProducer]
    #21737067 - 05/29/15 02:24 PM (8 years, 9 months ago)

So before I can determine if my LC is healthy I have to wait until cakes are done?
Under my time parameters It may not be viable to wait to fruit the cakes. The real treasure is in the bulk.

Your saying mold and bacteria are harboring inside the mycelium ?
Fruiting conditions won't be introduced until ....the substrate has consolidated?

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OfflinePinPornProducer
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Re: The Road to 7 mono tubs [Re: Syntheticwords] * 1
    #21737092 - 05/29/15 02:29 PM (8 years, 9 months ago)

It's the same as bulk, grains look healthy and clean but once spawned and fruited, if the spawn was bad, 3-4 days later it tams out. That's why we use agar, contams can't hide. Trich mycelium hides very well within mushroom myc. If you don't want to wait until fruits then test it on agar, you will know within a few days to a week if it's clean or not

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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,830
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Re: The Road to 7 mono tubs [Re: PinPornProducer] * 1
    #21737104 - 05/29/15 02:32 PM (8 years, 9 months ago)

FYI its best to test LC on agar. You can see right away on the 2D media if its clean or not. Its also better to inoculate LC with agar wedges anyway so the two go hand in hand IMO.

Given that its nearly impossible to have a 100% aseptic LC you need to use it as soon as you can after colonization. Agar gives an edge there for sure.

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OfflineSyntheticwords
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Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
Re: The Road to 7 mono tubs [Re: Pastywhyte]
    #21737137 - 05/29/15 02:42 PM (8 years, 9 months ago)

Currently the LC is on its 10th day

And agar would probably take a while to ship here
As a future reference those helps tremendously

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InvisiblePastywhyteMDiscord
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21737196 - 05/29/15 02:54 PM (8 years, 9 months ago)

I buy agar in an asian super market for cheap. Add your own nutes and its done. It can also be found in health food stores.

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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21739127 - 05/29/15 10:57 PM (8 years, 9 months ago)

Quote:

Syntheticwords said:
So before I can determine if my LC is healthy I have to wait until cakes are done?
Under my time parameters It may not be viable to wait to fruit the cakes. The real treasure is in the bulk.




Then wait the proper amount of time. Rushing things leads to failure, especially when you're new enough to have to ask these questions.

Trust me, putting shit off 2 weeks is much better than throwing away 7 tubs' worth of shit. Learn from my mistake, not yours.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflinePhosCap
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Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 10 months, 11 days
Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21739203 - 05/29/15 11:13 PM (8 years, 9 months ago)

I remember reading last year or so Frank went balls deep in LC's and then ultimately lost many tubs due to that unknown factor of contam. Then he bypassed and went straight to agar.  I personally never done LC because of what i read. Agar seems the best, easiest route.


--------------------
Psychoactive Fungi of the World        TLs            Franks teks and Tips        Stonesun's..
Spitballs Quick Rye Prep

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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: The Road to 7 mono tubs [Re: PhosCap]
    #21739218 - 05/29/15 11:17 PM (8 years, 9 months ago)

I've done lots and lots of LC. There's a reason I quit. If it works you have a fuckload of inoculant ready at once. A couple quarts of LC does a LOT of jars or bags. If it doesn't work, or you happen to contaminate it during a transfer, you lose just as much. Not worth it. Methods need to work 100% of the time for 100% of people who take time to do it properly.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
OfflineSyntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 11 months
Re: The Road to 7 mono tubs [Re: 36fuckin5]
    #21752126 - 06/02/15 10:33 AM (8 years, 9 months ago)

UPDATE:

Brf cakes are showing signs of healthy growth
If I hadn't posted on this forum I would probably
Feel extremely confident

However. Many of you make me question my work
I will heed your warnings and order a pack of petri agar dishes

Pics coming soon

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Invisibleslapphappypill
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Registered: 11/07/10
Posts: 5,570
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Re: The Road to 7 mono tubs [Re: Syntheticwords]
    #21752334 - 06/02/15 11:32 AM (8 years, 9 months ago)

next stop on that road, 100 monotubs :-P

good luck and keep posting pics/questions as needed!


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineBurberrySpores
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Registered: 08/05/14
Posts: 1,013
Last seen: 6 hours, 12 minutes
Re: The Road to 7 mono tubs [Re: slapphappypill]
    #21752453 - 06/02/15 12:02 PM (8 years, 9 months ago)

Lol.  I'm working on 20 right now:lookslucrative:


--------------------
I could go crazy... and no one would notice :seeya:

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Shop: North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals CBD Concentrates   MagicBag.co Certified Organic All-In-One Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Kratom Capsules for Sale


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