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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: blindingleaf]
    #21629325 - 05/03/15 09:51 AM (8 years, 10 months ago)

Yea I use new lids and rings each time.

That last plate was an agar transfer. It took a tumble and the agar I dropped to it tumbled around in there and I flicked the side till it was re centered.
So in lame terms this is the result of shaking up your plates after dropping the agar.
Here's two plates side by side.
Both transferred 4/18

One shaken one not... I'm not suggesting shaking up agar but you can see how nuts it went after it took a tumble.

Ps. Sorry for shit quality photos.

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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: help me with rye questions [Re: Thoky.ykoht]
    #21629334 - 05/03/15 09:55 AM (8 years, 10 months ago)

You just have an endless supply of rings you can use?


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: Buck513]
    #21629368 - 05/03/15 10:02 AM (8 years, 10 months ago)

All my weed comes in jars.
I've acquired many rings over the years.
New lids are a 1$ for 12 so for 10$ that's 120 lids.
Most expensive part is the glass, good thing ounces come in quart size jars haha

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: Thoky.ykoht]
    #21635404 - 05/04/15 03:47 PM (8 years, 10 months ago)

Tiger dropped that beast and five other plates, should I shake them as instructed in the tek?


Tossed the rest in ziplocks and threw them in the fridge.

Edited by Thoky.ykoht (05/04/15 03:48 PM)

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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: help me with rye questions [Re: Thoky.ykoht]
    #21635448 - 05/04/15 03:55 PM (8 years, 10 months ago)

Always shake after inoc (regardless of what the inoculant is) to help spread the inoc points.
A grain jar only needs to be shaken twice in its lifetime:wink:


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Fail to plan and you plan to fail.

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: Buck513]
    #21635522 - 05/04/15 04:10 PM (8 years, 10 months ago)

Done

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OfflinetaGyo
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Registered: 10/16/14
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Re: help me with rye questions [Re: Thoky.ykoht]
    #21635584 - 05/04/15 04:23 PM (8 years, 10 months ago)

Sup Thok,

Big chunks of agar you got there...


--------------------
Gyo's Better Grows
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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: taGyo]
    #21635594 - 05/04/15 04:26 PM (8 years, 10 months ago)

I tiger dropped my pasty plates.

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OfflinetaGyo
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Re: help me with rye questions [Re: Thoky.ykoht]
    #21635596 - 05/04/15 04:27 PM (8 years, 10 months ago)

That growth is a little weird... No one commented on that yet?


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: help me with rye questions [Re: taGyo]
    #21635607 - 05/04/15 04:28 PM (8 years, 10 months ago)

Quote:

taGyo said:
That growth is a little weird... No one commented on that yet?



I thought the same, I just figured it was from ms so it looked funky,lol


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OfflinetaGyo
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Re: help me with rye questions [Re: Thoky.ykoht]
    #21635624 - 05/04/15 04:32 PM (8 years, 10 months ago)

That looks like my contamed syringes from [Insert Shitty Vendor Here] Do. Can never get good growth. I assume you dropped from MS spore and just let those go?

They look like they're fighting a contam now. Time will tell but I would have transferred away at least once or twice until I clearly had good myc.
Quote:

Thoky.ykoht said:







One on the right looks OK but still a little sketch, the one on the left looks fucked.

We'll see though.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: Buck513]
    #21635689 - 05/04/15 04:42 PM (8 years, 10 months ago)

The plates I used were from a plate i got from a trade. I transferred the plate to 7 jars and 12 plates. No signs of contam so far. I just think it's from the agar i dropped tumbling around inside the pasty plate and breaking into smaller pieces causing the myc to grow out from several spots on the plate.
I mean we can all sit here and debate contam or not but won't know until it starts to recover in the jar.

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OfflinetaGyo
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Re: help me with rye questions [Re: Thoky.ykoht]
    #21635711 - 05/04/15 04:45 PM (8 years, 10 months ago)

:thumbup:


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Gyo's Better Grows
TNF Q&A :rockon:
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InvisiblebodhisattaMDiscordReddit
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Re: help me with rye questions [Re: taGyo]
    #21638219 - 05/05/15 05:06 AM (8 years, 10 months ago)

If a wedge moves around on agar before settling down in one spot you'll see weird growth patterns

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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: bodhisatta]
    #21643718 - 05/06/15 11:06 AM (8 years, 10 months ago)

So when do I shake these again?
Jars from 4/15 and 4/18

Here's the jars from 4/21 starting to recover I shook them two days ago.

And here's the jars with the tiger drop...
Only signs of growth so far is the myc recovering on the edge of a piece of agar.


Edited by Thoky.ykoht (05/06/15 11:07 AM)

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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: help me with rye questions [Re: Thoky.ykoht]
    #21644260 - 05/06/15 02:10 PM (8 years, 10 months ago)

When its like 20-30% colonized.


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: Buck513]
    #21665913 - 05/11/15 03:47 PM (8 years, 10 months ago)

Here's an update....

4/15: Few more days!

4/18: Few more days!

4/21: Considering shaking again

5/4 tiger drop: Let the plates recover both side and shook again today.
Each plate resembled the the third photo when uncovering them while shaking.


What should my spawn to sub ratio be when making up a 44qt tub?
I found some of these weatherized tubs, perfect for monotubes.

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OfflinetaGyo
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Re: help me with rye questions [Re: Thoky.ykoht]
    #21669057 - 05/12/15 09:14 AM (8 years, 10 months ago)

I'd do 4-5 in those 44qts.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: help me with rye questions [Re: taGyo]
    #21673755 - 05/13/15 08:16 AM (8 years, 10 months ago)

Hey so I was looking over jars from 4/21 and I came upon these spots that seem to be healthy myc bluing but only in tiny spot in 4 separate jars I don't want to jump the gun and toss them because I've read over several other people who experience something similar while there jars were recovering from a shake.
I've even see RR saying "It's common for some strains to bruise blue after shaking the jar. It usually goes away after a few days"

Here's some shots of the culprits

Edited by Thoky.ykoht (05/13/15 08:17 AM)

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InvisiblebodhisattaMDiscordReddit
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Re: help me with rye questions [Re: Thoky.ykoht]
    #21673831 - 05/13/15 08:45 AM (8 years, 10 months ago)

looking good

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