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 FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 3 hours
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 Additive Experiment 
#21458368 - 03/26/15 01:26 AM (9 years, 23 days ago) |
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I've done some browsing and have seen ideas of different additives - but people didn't follow through... So here I am taking it upon myself.
My two variables are the fact that I'm using a ms syringe and the additives used in my simmer:
1. 1000mg of vit C with 150mg of rose hips
2. 1/2 teaspoon of organic, raw honey
3. 1/2 teaspon of extra virgin cocon oil, cold pressed
4. .05oz of organic ginger w/chamomile
Ignore the coffee, was going to use it but I want to try something different.
My controls are as follow:
-5qt jars, each with 1 & 1/4 cups of WBS
-10 cups of water for simmer
- All will be PC'd and inoculated in the same batch with same syringe.
-Same shelf, temp, etc.. Etc...
My main goal in this is to see if these additives have a positive or negative effect on the growth rate of mycelium within the jars.
I will proceed to compare side by side with colinization of sub and fruiting pending results.
Edited by FeedYourMind (03/26/15 03:45 AM)
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 R0ughHab1tz
drop the hardstyle!!
Registered: 06/07/09
Posts: 216
Loc: Canada
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Are you using spores or LC? And if your using LC did you isolate until you got a single sector?
Going to be following for sure but if your using spores or didn't isolate, the results might not give a definitive answer
--------------------
my Android eats Blackberrys and shits Iphones
proper WBS tek - 2 ingredient 4 step LC TEK (noobafied)
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StygianKnight
A Mushroom
Registered: 03/12/12
Posts: 2,717
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Why did you choose these additives?
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FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 3 hours
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Quote:
djbluephoenix said:
Are you using spores or LC? And if your using LC did you isolate until you got a single sector?
Going to be following for sure but if your using spores or didn't isolate, the results might not give a definitive answer
Quote:
FeedYourMind said:
My two variables are the fact that I'm using a ms syringe and the additives used in my simmer.
I don't have an isolate as of yet, I need to get some supplies for agar.
Fortunately I already have some qt jars going from his same syringe and their growth is nearly identical. 
But yes, the correct way would be to isolate. I plan on doing a part 2 to follow up with an isolate to either confirm or deny my findings with an ms syringe.
Part of me hopes that if I have a consistent ms syringe or print, that it can be used for testing next to isolates, but I know that's a high hope.
Edited by FeedYourMind (03/26/15 02:24 AM)
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FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 3 hours
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Quote:
StygianKnight said:
Why did you choose these additives?
Great question.
I'm just curious and would love to help give back to the community with new discoveries.
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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this could be off, but i think coconut oil/extract/milk/juice might be anti fungal?
stoner memory, u dig? 
i read it in terms of agar additives though.
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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 3 hours
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Quote:
blindingleaf said:
this could be off, but i think coconut oil/extract/milk/juice might be anti fungal?
stoner memory, u dig?
i read it in terms of agar additives though.
That's interesting, and I think you're right. I used to have seborrheic dermatitis on my face and I use to use coconut oil for relief.
I don't even know if the mycelium will even like a fatty substance like this or olive oil.
We shall see, I have another two jars of spawn to simmer before I pc and get to inoculation.
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Albertheisenhouer
Tryptamine doctor
Registered: 10/26/13
Posts: 734
Loc: Holland
Last seen: 3 days, 21 hours
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I would use at least 5 replicates. I have experienced a big standard deviation in yields when using the same technique. Using only 1 sample will likely give you false results or interpretations
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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fats/lipids are ok, just in moderation.
have not read about olive oil. mainly soy oil.
--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 3 hours
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Quote:
Albertheisenhouer said:
I would use at least 5 replicates. I have experienced a big standard deviation in yields when using the same technique. Using only 1 sample will likely give you false results or interpretations
Damn, great point.
Luckily I have more jars, but not enough to even go 3 on each. But maybe two..
So I'm starting to see the flaws in my plan thanks to you fine people, so maybe I can expand on this when I get my isolate ready and have it at full force experiment/tek.
Quote:
blindingleaf said:
fats/lipids are ok, just in moderation.
have not read about olive oil. mainly soy oil.
That's good to hear, and I figured 2.5ml to 10 oz. of water would suffice as a low ratio.
I know this may not be indicative of a great experiment, but it is one nonetheless.
Curiosity... I just have to try it all.
Or learn more about the science behind mycelium.
Which reminds me, I need to go buy some books.
Thanks Fam!
Side note - I predict the tea spawn will do best. Just because of its organic nature as an herb.
Edited by FeedYourMind (03/26/15 03:21 AM)
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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books are good, but growing is better 
good luck man!
--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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FeedYourMind
indiGlo
Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 3 hours
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Quote:
blindingleaf said:
books are good, but growing is better
good luck man!
I know that's the truth!
When I first started growing a few years ago, I lurked as a guest on these forums and read as much as I could for a month before I even gathered supplies.
I'm glad I read though because looking back, I could have wasted my time and money on a complete and utter failure - because there were times, even feeling confident for my first grow, that I nearly messed up.
And thanks dude! I'll keep everyone updated. 
Update: All jars nocc'd up.
Edited by FeedYourMind (03/26/15 01:24 PM)
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