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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: Agar plauged by bacteria [Re: NumeroEno]
    #21361825 - 03/04/15 03:34 PM (9 years, 26 days ago)

Sounds very good, thanks for all the input everyone. This was the third transfer from spores and still seeing bacteria. Ill whip up a new batch today and hopefully ill be able to use the next transfer to inoculate some grains


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OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,866
Last seen: 20 days, 8 hours
Trusted Cultivator
Re: Agar plauged by bacteria [Re: JvF]
    #21361860 - 03/04/15 03:41 PM (9 years, 26 days ago)

I had a bacteria colony on one of those plates (agar+potato broth) and the colony didn't even grow. So I did an experiment and took my unsterile blade and touched it to the agar. A bacteria colony formed where I had touched it but once again the colony remained localized, it didn't even colonize the plate at least not visibly.


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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: Agar plauged by bacteria [Re: Kizzle]
    #21361896 - 03/04/15 03:49 PM (9 years, 26 days ago)



Im seeing alot of what youre describing with regard to the localized colonies. In one case, the myc even ate the colony completely. That was the transkei strain and is some of the most aggressive myc ive ever seen. The fluffy parts were all where the bacteria was and as you can see, once it 'ate' the bacteria, its grown incredibly healthy


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InvisibleVal
Were just Marbles in a Cave...

Registered: 01/31/15
Posts: 120
Re: Agar plauged by bacteria [Re: JvF]
    #21362171 - 03/04/15 04:37 PM (9 years, 26 days ago)

Cut out that ropey part and transfer to a few plates.
Only need a small chunk 1-2mm x 1-2mm @3,5,6 oclock

Also just a question is there any condensation on your plate lids?

Edited by Val (03/04/15 04:44 PM)

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InvisibleJvF
Fletcher Detcher


Registered: 02/13/14
Posts: 2,662
Loc: Chicago Flag
Re: Agar plauged by bacteria [Re: Val]
    #21362238 - 03/04/15 04:55 PM (9 years, 26 days ago)

Yea im planning on tranafering in a few hours :laugh:

and yea, usually for thr top 2 or so of the stadk theres condensation but it disappears by the next day or so


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