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 Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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 Re: Cactus tissue culture.  [Re: Una]
#2107117 - 11/14/03 04:47 PM (6 years, 8 days ago) |
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Ok here's a good pic of the san pedro. Most all of the areoles are swelling and sprouting new shoots. It's more than a week faster than the initial explants reacted. The plants are adapting to their new environment 
And some lophophora williamsii.
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www.911blogger.com
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#2135231 - 11/25/03 02:40 PM (5 years, 11 months ago) |
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just a little update:
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 stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Loc: Sweden
Last seen: 3 months, 19 days
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Re: Cactus tissue culture. [Re: Una]
#2135247 - 11/25/03 02:50 PM (5 years, 11 months ago) |
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Keep em comming Una..this is sooo cool.
Looking forward to more updates..
peace..
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medicinebag
Hunting
Registered: 11/15/03
Posts: 344
Loc: The land of The People
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What kind of Base is the culture setting in?
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,604
Loc: Rigel 7
Last seen: 15 days, 11 hours
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Re: Cactus tissue culture. [Re: medicinebag]
#2136592 - 11/26/03 12:09 AM (5 years, 11 months ago) |
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the explants are on agar with nutrition and hormones
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#2227778 - 01/08/04 08:53 AM (5 years, 10 months ago) |
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These pic were taken a few weeks ago. The pachanoi is growing very nice in tissue culture.
Group photo
Close-up
Loph. williamsii callus cutlure with spontaneous button formation.
Their habitat 
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www.911blogger.com
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DazedSol
old hand
Registered: 08/01/01
Posts: 1,230
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Re: Cactus tissue culture. [Re: Una]
#2229031 - 01/08/04 05:14 PM (5 years, 10 months ago) |
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Very very nice!!!
Wish i had time to experiment more.........ever try tissue culture of cannabis??
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Peace,
Adam
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Openminded
Dicotyledon
Registered: 08/28/03
Posts: 657
Loc: England.
Last seen: 1 year, 6 months
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Re: Cactus tissue culture. [Re: DazedSol]
#2230450 - 01/09/04 05:46 AM (5 years, 10 months ago) |
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For anyone that's interested in tissue culture, this page:
http://www.oup.co.uk/pdf/bt/slater/ch02.pdf
Might be a good starting point. It gives the composition of a typical culture medium, briefly describes the roles of the different growth regulators, gives examples of them, etc.
Edit: the ratios etc that they give surely won't be optimised for cacti, but I still think it's a page that is worth reading...
Edited by Openminded (01/09/04 05:50 AM)
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angryjslice
now with 20%more anger
Registered: 10/29/03
Posts: 916
Last seen: 10 months, 8 days
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Re: Cactus tissue culture. [Re: Una]
#2267795 - 01/24/04 07:56 AM (5 years, 9 months ago) |
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bump/question
this thread is amazing
una, do you forsee any problems in transfering these back out of the liquid medium there in into soil?
~JSlice~
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,604
Loc: Rigel 7
Last seen: 15 days, 11 hours
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Re: Cactus tissue culture. [Re: angryjslice]
#2268288 - 01/24/04 01:09 PM (5 years, 9 months ago) |
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There should be no problems what so ever. In a class i took some time ago, we got these african violets with chimera's on them. We isolated the tissue that was exhibiting the chimera and grew out african violets on the culture then transfered back to soil. No problems. Unfortunately the chimera's didn't last and the violets turned green again after some time.
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YSr
Registered: 04/13/04
Posts: 81
Last seen: 3 years, 4 months
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Re: Cactus tissue culture. [Re: neuro]
#2935660 - 07/28/04 08:10 AM (5 years, 3 months ago) |
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Is it possible to grow Salvia the same way?
What kind of media should I use then?
YSr
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,604
Loc: Rigel 7
Last seen: 15 days, 11 hours
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Re: Cactus tissue culture. [Re: YSr]
#2936577 - 07/28/04 02:15 PM (5 years, 3 months ago) |
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>>Is it possible to grow Salvia the same way?
Yes.
>>What kind of media should I use then?
Agar as the mediu, and proper phytonutrients and most important hormones. Plant Tissue culture is quite a complex thing compared average horticulture so much so that most colleges devote whole classes usually 101/102 and upper level tissue culture 300.
I suggest getting a book and startting from there and also a good supplier of IAA, GA, and the like. Because it all depends on the ratio of plant hormones as to whether you get roots, shoots, or both, or a callus .
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Stonehenge
enthusiast
Registered: 06/20/04
Posts: 2,056
Last seen: 2 hours, 31 seconds
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Re: Cactus tissue culture. [Re: neuro]
#2936801 - 07/28/04 03:02 PM (5 years, 3 months ago) |
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I think I'd like to take one of those classes. Another one I'd like to take is photoshop. The photoshop tutorials are just about worthless. I'll check to see if any tissue culture classes are offered in my area but I doubt it.
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: YSr]
#2936954 - 07/28/04 03:39 PM (5 years, 3 months ago) |
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Salvia can be grown in tissue culture quite easily. But.....my medium recipe is a secret, that's the way things are in tissue culture land...No one shares their recipes and it's often a long trial and error process to get some decent growth.
Here are two pics...
This plantlet has just started to root...
These guys are ready to be transplanted to soil.
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www.911blogger.com
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YSr
Registered: 04/13/04
Posts: 81
Last seen: 3 years, 4 months
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Re: Cactus tissue culture. [Re: Una]
#2939467 - 07/29/04 05:30 AM (5 years, 3 months ago) |
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But could you please give a light introduction of some ingredients
to start experimenting with?
And please give a short description of what IAA and GA is....
YSr
Edited by YSr (07/29/04 05:32 AM)
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: YSr]
#2939491 - 07/29/04 05:56 AM (5 years, 3 months ago) |
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IAA and GA3 are plant growth regulators. They signal the plant to development in a certain way; shoots, roots, callus etc.
A small introduction to plant tissue culture can be found here.
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www.911blogger.com
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YSr
Registered: 04/13/04
Posts: 81
Last seen: 3 years, 4 months
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Re: Cactus tissue culture. [Re: Una]
#2939507 - 07/29/04 06:18 AM (5 years, 3 months ago) |
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Do you have some more links to pages where I can read about diffrent
mediums for plant tissue cultures?
I'm very interested and don't know where to start. Love working with
shroom-cultures om agar and liquid karo.
YSr
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: YSr]
#2939550 - 07/29/04 06:56 AM (5 years, 3 months ago) |
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www.911blogger.com
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faslimy
Dead Man
Registered: 04/04/04
Posts: 3,435
Last seen: 11 days, 11 hours
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Re: Cactus tissue culture. [Re: Una]
#2946673 - 07/30/04 10:28 PM (5 years, 3 months ago) |
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There is another group of people who decided to keep their findings to themselves, they enslaved humanity for world domination. Why keep everyone in the dark on the best methods for things?
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eric_the_red
Registered: 02/28/03
Posts: 12,638
Loc: happy land
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Re: Cactus tissue culture. [Re: faslimy]
#2946717 - 07/30/04 10:40 PM (5 years, 3 months ago) |
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i don't see how una's secret formula compares to enslaving humanity in any way. una has a lot of money, time, research, and hard work invested in his business. he is not obligated to give away what he has worked so hard to find. there is nothing wrong with reaping the benefits of your hard work.
una has pointed you in the right direction. if you are really interested, do some research and follow the path that you have been shown.
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Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave
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