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 blazedup
Dirk Diggler
 
Registered: 06/05/11
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 first attempt with agar 
#21243812 - 02/08/15 01:07 PM (9 years, 2 months ago) |
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So I'm finally going to attempt agar work. Hopefully I can manage to do it right to knock up some grain.
2/17 update...took some pictures of one of the agar cultures I used a really old syringe on.
     
Edited by blazedup (02/17/15 07:24 PM)
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21243993 - 02/08/15 01:42 PM (9 years, 2 months ago) |
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Sorry it ended upside down
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mushpunx
Fungus Punk
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Re: first attempt with agar [Re: blazedup]
#21244063 - 02/08/15 02:02 PM (9 years, 2 months ago) |
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This is where the real fun starts!
Agar is actually one of the more forgiving parts of this hobby, you can recover from mistakes.
As long as you practice your hand motions and mind what you're doing in your still box you do fine man!
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Amateur Mycologists United
AMU Q&A
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: mushpunx]
#21244090 - 02/08/15 02:08 PM (9 years, 2 months ago) |
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Quote:
mushpunx said:
This is where the real fun starts!
Agar is actually one of the more forgiving parts of this hobby, you can recover from mistakes.
As long as you practice your hand motions and mind what you're doing in your still box you do fine man!
It was intimidating to finally try it but I can't wait to see what comes from it. I assume this also makes it easier to use a wedge then waste a syringe.
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 NumeroEno
I come from the land of lizards
Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge 
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Re: first attempt with agar [Re: blazedup]
#21244154 - 02/08/15 02:26 PM (9 years, 2 months ago) |
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Agar is the shit. I've been using it since about September and I'm never looking back.
Good luck!
--------------------
  Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Grey
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Posts: 6,223
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Re: first attempt with agar [Re: NumeroEno]
#21244344 - 02/08/15 03:21 PM (9 years, 2 months ago) |
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I just started agar too. Just waiting on some germination.
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  AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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mushpunx
Fungus Punk
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Re: first attempt with agar [Re: blazedup]
#21244358 - 02/08/15 03:25 PM (9 years, 2 months ago) |
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Quote:
blazedup said:
Quote:
mushpunx said:
This is where the real fun starts!
Agar is actually one of the more forgiving parts of this hobby, you can recover from mistakes.
As long as you practice your hand motions and mind what you're doing in your still box you do fine man!
It was intimidating to finally try it but I can't wait to see what comes from it. I assume this also makes it easier to use a wedge then waste a syringe.
Yea man you can keep a culture alive into perpituity almost
You can never be sure a print or syringe is truly clean, especially if you do a bigger grow you want to be confident of your spawn!
Post us on your progress
--------------------
Amateur Mycologists United
AMU Q&A
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
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Re: first attempt with agar [Re: mushpunx]
#21244366 - 02/08/15 03:28 PM (9 years, 2 months ago) |
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Quote:
mushpunx said:
Quote:
blazedup said:
Quote:
mushpunx said:
This is where the real fun starts!
Agar is actually one of the more forgiving parts of this hobby, you can recover from mistakes.
As long as you practice your hand motions and mind what you're doing in your still box you do fine man!
It was intimidating to finally try it but I can't wait to see what comes from it. I assume this also makes it easier to use a wedge then waste a syringe.
Yea man you can keep a culture alive into perpituity almost
You can never be sure a print or syringe is truly clean, especially if you do a bigger grow you want to be confident of your spawn!
Post us on your progress
After coming out the pc. When do I drop some spores in?
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mushpunx
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Re: first attempt with agar [Re: blazedup]
#21244412 - 02/08/15 03:40 PM (9 years, 2 months ago) |
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As soon as the agar is cool and set dude, couple hours maybe?
You can see the color changing, I say give a little extra time your first shot
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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Re: first attempt with agar [Re: blazedup]
#21244420 - 02/08/15 03:42 PM (9 years, 2 months ago) |
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if it is your first time maybe try transferring spores to many plates in case some fail
and have some more agar on hand for when the first transferable growth shows up
--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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blazedup
Dirk Diggler
Registered: 06/05/11
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Quote:
filthyknees said:
if it is your first time maybe try transferring spores to many plates in case some fail
and have some more agar on hand for when the first transferable growth shows up
Ok so once there is some good growth cut it and put it into another new plate?
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21244601 - 02/08/15 04:37 PM (9 years, 2 months ago) |
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Yep, exactly.
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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Re: first attempt with agar [Re: Psilicon]
#21244665 - 02/08/15 04:56 PM (9 years, 2 months ago) |
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--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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NumeroEno
I come from the land of lizards
Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Quote:
filthyknees said:
if it is your first time maybe try transferring spores to many plates in case some fail
and have some more agar on hand for when the first transferable growth shows up
Yes. I'm a huge fan of making way more plates than I need. It's kinda hard to spot contams on no-pour agar, so it's wise to have as many plates as you can make. I just poured 20 plates and inoculated 14 of them. Only 4 different kinds of mushrooms. The other 6 are all gonna be for this PE I have pinning right now.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: NumeroEno]
#21244839 - 02/08/15 05:30 PM (9 years, 2 months ago) |
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That's very thorough. I only do two plates at a time, unless it's a clone, in which case I go wild and do three.
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mushpunx
Fungus Punk
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Re: first attempt with agar [Re: Psilicon]
#21245018 - 02/08/15 06:07 PM (9 years, 2 months ago) |
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My inoculation are usually one or two plates, sometimes I use no pour for the first plates, but really I try not to inoculate too many plates cause I only need a tiny clean piece and then it gets tossed. I hate waste
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NumeroEno
I come from the land of lizards
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Posts: 9,652
Loc: Gamehendge
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Re: first attempt with agar [Re: mushpunx]
#21245043 - 02/08/15 06:12 PM (9 years, 2 months ago) |
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Quote:
mushpunx said:
My inoculation are usually one or two plates, sometimes I use no pour for the first plates, but really I try not to inoculate too many plates cause I only need a tiny clean piece and then it gets tossed. I hate waste
I've been having a lot of contams lately, but I'm also weeding through a lot of genetics. Every last bit of clean agar I have will get used in some way though. I hear LI keeps pretty well in the fridge too.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: NumeroEno]
#21245127 - 02/08/15 06:34 PM (9 years, 2 months ago) |
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I messed up because I was doing too much. So I knocked up 3 but 2 are ksss and the 1 is an old brazil. Forgot to label them 
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21270948 - 02/13/15 09:53 PM (9 years, 2 months ago) |
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So I'm finally seeing some growth but its tough to see because of the type of container they are in. Is it possible to put them in a SAB to take to tops off to check status?
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mushpunx
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Re: first attempt with agar [Re: blazedup]
#21271039 - 02/13/15 10:16 PM (9 years, 2 months ago) |
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Quote:
blazedup said:
So I'm finally seeing some growth but its tough to see because of the type of container they are in. Is it possible to put them in a SAB to take to tops off to check status?
As soon as you see growth you wanna make your first transfer.
You could open them up yea but you dont want to leave the lid off more than a few seconds. But if you see growth its a safe bet its time to take first transfers
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trippyshroom
Bacterial genius
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Re: first attempt with agar [Re: mushpunx]
#21271277 - 02/13/15 11:27 PM (9 years, 2 months ago) |
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I absolutely love agar I'm still a total noob at it but right now I got some Cambodian starting on agar and PE and a Brazilian clone.
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Anything that is posted above is just a figment of your imagination.
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blazedup
Dirk Diggler
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Sorry for the poor pictures but my phone couldn't seem to focus correctly and I didn't want to chance contams from having it open for more then a couple seconds.
   
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21273040 - 02/14/15 12:13 PM (9 years, 2 months ago) |
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Doesn't look all good to me, you need better pics though.
How many drops did you do? Did you move the plate directly after dropping it, causing the drop to move around and spread spores everywhere?
You're looking for pure white:
The milky stuff is bacteria:
This is cubensis:
--------------------
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21273070 - 02/14/15 12:20 PM (9 years, 2 months ago) |
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Quote:
taGyo said:
Doesn't look all good to me, you need better pics though.
How many drops did you do? Did you move the plate directly after dropping it, causing the drop to move around and spread spores everywhere?
You're looking for pure white:
The milky stuff is bacteria:
This is cubensis:
Unfortunately I ended up putting more then a drip in there and when putting it away it spread around because too much liquid
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21273076 - 02/14/15 12:21 PM (9 years, 2 months ago) |
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Ahh,
Watch it then. Transfer myc at the first signs of healthy growth.
--------------------
Gyo's Better Grows
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: taGyo]
#21273077 - 02/14/15 12:22 PM (9 years, 2 months ago) |
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I think the biggest blotch, which he seems to be centered on, looks okay. I'd transfer from there now. I agree with tag that it looks like it spread around, though.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21273088 - 02/14/15 12:26 PM (9 years, 2 months ago) |
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Ok I'll make some new dishes ASAP...and make a loop to transfer. Does it make sense to cut the piece out to transfer or just scrape the top?
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21273106 - 02/14/15 12:31 PM (9 years, 2 months ago) |
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I've heard scraping works from people I respect, but haven't tried it so I can't recommend it personally.
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spacechildo
proletarians rise up
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Re: first attempt with agar [Re: Psilicon]
#21273481 - 02/14/15 02:23 PM (9 years, 2 months ago) |
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I wouldn't recommend it but I've done it when the leading edges look bacterial,
just lightly touch an area where the cube myc has "risen" a bit above the plate
and touch it again on your new plate.
I've only done it a handful times but still considered every success to be "luck".
You should try it vis a vis the "nothing to lose" theorY!
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blazedup
Dirk Diggler
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Quote:
spacechildo said:
I wouldn't recommend it but I've done it when the leading edges look bacterial,
just lightly touch an area where the cube myc has "risen" a bit above the plate
and touch it again on your new plate.
I've only done it a handful times but still considered every success to be "luck".
You should try it vis a vis the "nothing to lose" theorY!
You don't recommend which option?
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spacechildo
proletarians rise up
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Re: first attempt with agar [Re: blazedup]
#21273540 - 02/14/15 02:38 PM (9 years, 2 months ago) |
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scraping the top of clean looking myc. I've done it with success a handful times but each time it has felt like luck.
Dont ask me how luck feels, just that I never expected it to work but yet it somehow did 
I'd rather start over, but as I stated, what have you got to lose? 
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blazedup
Dirk Diggler
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So I did the transfer using the scrape method...after getting a better look in the jar there was a nice growth going on. The second one I opened looked like straight bacteria because it was cloudy white spots and just didn't look right. I also did my first print to agar transfer.
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Grey
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Re: first attempt with agar [Re: blazedup]
#21276857 - 02/15/15 11:00 AM (9 years, 2 months ago) |
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I did print to agar. The first ones took 7-8 days to show any signs of growth. I made up some more plates, but cut back .5g on the agar giving it a more "preserve" look rather than a stiff wax. I used some of the same spores I used on the first plates on the new ones, as well as some GT and Texan that was printed last month. I'm hoping the more aqueous agar and fresher spores will take off sooner. That and I used a scalpel instead of a sterile swab so I got a ton of spores put down.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Grey]
#21276883 - 02/15/15 11:05 AM (9 years, 2 months ago) |
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Quote:
ingclassy said:
I did print to agar. The first ones took 7-8 days to show any signs of growth. I made up some more plates, but cut back .5g on the agar giving it a more "preserve" look rather than a stiff wax. I used some of the same spores I used on the first plates on the new ones, as well as some GT and Texan that was printed last month. I'm hoping the more aqueous agar and fresher spores will take off sooner. That and I used a scalpel instead of a sterile swab so I got a ton of spores put down.
Nice work..any pics? I want to figure out a better agar mix because the potato agar seems to come out very thick and the color ain't the greatest. I prefer something more transparent
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Grey
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Re: first attempt with agar [Re: blazedup]
#21276953 - 02/15/15 11:15 AM (9 years, 2 months ago) |
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I cant get them to upload. I'm switching phones today, maybe my new one won't suck. I made a double batch of pasty plates so the mix, with reduces agar, ended up being:
1 cup water,
6.6g potato flakes,
4.5 grams agar powder (would have been 5 if I had used the same amount as the first batch), and 3.1g honey.
I used food coloring but only three drops so it came out almost pink. Everyone says the mycelium eats up the red so I used that to kinda measure growth from the bottom.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Grey
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Re: first attempt with agar [Re: Grey]
#21277555 - 02/15/15 01:08 PM (9 years, 2 months ago) |
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Here we go:
   
You can't see that well, but I can tell where I rolled the swab because spores germinated in different spots. It took a while to germinate, but they're almost doubling in size every day now.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
Edited by Grey (02/15/15 01:14 PM)
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taGyo
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Re: first attempt with agar [Re: Grey]
#21277569 - 02/15/15 01:10 PM (9 years, 2 months ago) |
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Three looks like a little bacteria in there. Two and four look fine, I think one might have a little bacteria as well.
Agar Bacteria:
Bacteria is kind of milky, whiteish. Looks like a pool of milky white water.
--------------------
Gyo's Better Grows
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Edited by taGyo (02/15/15 01:13 PM)
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Grey]
#21277586 - 02/15/15 01:12 PM (9 years, 2 months ago) |
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Quote:
ingclassy said:
Here we go:
Thanks for posting the pics
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21277591 - 02/15/15 01:13 PM (9 years, 2 months ago) |
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I'm starting to really like agar
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21277604 - 02/15/15 01:14 PM (9 years, 2 months ago) |
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Quote:
taGyo said:
Bacteria is kind of milky, whiteish. Looks like a pool of milky white water.
Yeah, or the plaque you scrape off your teeth. 
Quote:
blazedup said:
I'm starting to really like agar
Good! I knew you would. 
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taGyo
Strainiac/AMU
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Re: first attempt with agar [Re: Psilicon]
#21277631 - 02/15/15 01:18 PM (9 years, 2 months ago) |
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What kind of shit do you eat bro?
--------------------
Gyo's Better Grows
TNF Q&A
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Dominus fortunae meae sum
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blazedup
Dirk Diggler
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Posts: 1,235
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Re: first attempt with agar [Re: Psilicon]
#21277682 - 02/15/15 01:26 PM (9 years, 2 months ago) |
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Quote:
van der griegen said:
Quote:
taGyo said:
Bacteria is kind of milky, whiteish. Looks like a pool of milky white water.
Yeah, or the plaque you scrape off your teeth.
Quote:
blazedup said:
I'm starting to really like agar
Good! I knew you would.
I think the real fun is going to be isolating and of course using the active myc to put on grain
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Grey
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Re: first attempt with agar [Re: blazedup]
#21277781 - 02/15/15 01:44 PM (9 years, 2 months ago) |
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Yeah, on 1 2 and 3 I rolled the swab. On 3 I was trying to press hard and sort of flicked it. There's three points on 3 that germinated, the center and two next to each other off to the side that are growing in a faint figure eight. 4 I actually just jabbed the swab right into the center of the agar. I was trying to see the consistency, which ended up being firm like wax. That's why I backed off half a gram on the new plates. I made enough in case there's a bad one.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Grey]
#21284995 - 02/16/15 07:10 PM (9 years, 1 month ago) |
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Just got a nice shipment in...
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taGyo
Strainiac/AMU
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Re: first attempt with agar [Re: blazedup]
#21285016 - 02/16/15 07:15 PM (9 years, 1 month ago) |
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Nice,
Pack of 500?
--------------------
Gyo's Better Grows
TNF Q&A
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Dominus fortunae meae sum
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21285065 - 02/16/15 07:24 PM (9 years, 1 month ago) |
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Quote:
taGyo said:
Nice,
Pack of 500?
250... I saw the deal for 500 but didn't know if that was too much
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taGyo
Strainiac/AMU
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Re: first attempt with agar [Re: blazedup]
#21285107 - 02/16/15 07:31 PM (9 years, 1 month ago) |
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I got 500 tri-petris.
Next time save the money and just get the 500 lol. You'll need them all if you plan on continuing down this path and judging by your registration date you do 
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Gyo's Better Grows
TNF Q&A
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Dominus fortunae meae sum
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21285356 - 02/16/15 08:28 PM (9 years, 1 month ago) |
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Quote:
taGyo said:
I got 500 tri-petris.
Next time save the money and just get the 500 lol. You'll need them all if you plan on continuing down this path and judging by your registration date you do
Lol funny u mention that because I was just noticing how long I been a member to the forum. Ur right though next time I'm doing the 500. I just saw my first sectors today on one of my plates
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21285371 - 02/16/15 08:32 PM (9 years, 1 month ago) |
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Congrats brother. It's all uphill from here 
--------------------
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NumeroEno
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Re: first attempt with agar [Re: taGyo]
#21285511 - 02/16/15 09:09 PM (9 years, 1 month ago) |
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It was very hard for me to spot bacteria in pasty plates. I've started using petris and now I can see all the bacteria. Now I know why I lost so many grain jars to bacteria.
I still have to give major props to pasty. I never would have started using agar without his tek. It's a great introduction to agar, but after doing a lot of both I definitely prefer poured petris.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
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Re: first attempt with agar [Re: NumeroEno]
#21297581 - 02/19/15 08:48 AM (9 years, 1 month ago) |
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I am about to finally make my first Petri dishes. I picked up some green food coloring as I read in a few threads helps spot contams. Should I use PDA or mea? I just ordered some light malt extract.
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21297683 - 02/19/15 09:09 AM (9 years, 1 month ago) |
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Both PDA and MEA are great. I recommend you use both in rotation, so whichever one it closer to hand.
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21298658 - 02/19/15 12:24 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Both PDA and MEA are great. I recommend you use both in rotation, so whichever one it closer to hand.
Got ya....either way I need to make some transfers so I'm not waiting until my malt extract comes in.
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blazedup
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Re: first attempt with agar [Re: blazedup]
#21300202 - 02/19/15 05:50 PM (9 years, 1 month ago) |
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These pics are from one of the transfers I made. I need to take out my slr to get a really clear picture.
Update 2/19:
    
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21300214 - 02/19/15 05:52 PM (9 years, 1 month ago) |
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Looking good. Is that a takeout container?
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21300276 - 02/19/15 06:12 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Looking good. Is that a takeout container?
Lol...They are close to the size of takeout containers. They may be a tiny bit over 2 inches tall. Dollar store gems. A buck for 6 and they are able to go in the pc.
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blazedup
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Re: first attempt with agar [Re: blazedup]
#21300637 - 02/19/15 07:23 PM (9 years, 1 month ago) |
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21300651 - 02/19/15 07:27 PM (9 years, 1 month ago) |
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Cool. So it looks like you might have gotten a little bit more than a drop of spore solution in there, and it rolled around. Am I right?
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21300678 - 02/19/15 07:31 PM (9 years, 1 month ago) |
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Yea ur def right...tried to put a drop and it didn't come out that way. I know you can't see detail in the picture but I'm curious if that's healthy growth or both bacteria/myc.
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21300683 - 02/19/15 07:32 PM (9 years, 1 month ago) |
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No clue! You're going to have to transfer a wedge either way, right? No big deal.
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21300692 - 02/19/15 07:35 PM (9 years, 1 month ago) |
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Lol I have no clue either. I guess I will be transferring some fun tomorrow. . . U see the 3rd and 4th picture? Any thoughts on that smaller one?
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21301083 - 02/19/15 08:56 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
No clue! You're going to have to transfer a wedge either way, right? No big deal.
Should I transfer wedge to another plate, LC or grain? Or all 3?
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NumeroEno
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Re: first attempt with agar [Re: blazedup]
#21301194 - 02/19/15 09:16 PM (9 years, 1 month ago) |
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Definitely just to another plate first. You want to be very sure your culture is clean before you try inoculating anything. Most of my failed grows are the result of dropping dirty wedges into grain.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Psilicon
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Re: first attempt with agar [Re: NumeroEno]
#21301385 - 02/19/15 09:48 PM (9 years, 1 month ago) |
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21302347 - 02/20/15 06:27 AM (9 years, 1 month ago) |
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Guess you can't appreciate agar without seeing all types of outcomes. It's a learning experience every time. Here are a few pics of confirmed bacteria from and old syringe. Or at least I think it's from the old syringe. Didn't get to label it when I did it.
   
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21302363 - 02/20/15 06:34 AM (9 years, 1 month ago) |
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That screams syringe to me. Your chances get a little better the less spore solution you use, so I'd practice letting out a single drop onto your SAB floor right before putting another single drop to agar.
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21302384 - 02/20/15 06:43 AM (9 years, 1 month ago) |
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Quote:
van der griegen said:
That screams syringe to me. Your chances get a little better the less spore solution you use, so I'd practice letting out a single drop onto your SAB floor right before putting another single drop to agar.
I noticed what else messed it up was the way the agar solidified. I must have had it off balance because its in a slanted position causing the syringe drop to slide down. I will practice with one of my used up syringes and some tap water. Better to get a full understanding of what's necessary by practicing. Good advice..thanks
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21302394 - 02/20/15 06:48 AM (9 years, 1 month ago) |
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A little trick you can do is to use the tip of your hot needle to burn a little hole into the agar. That gives the drop a place to sit without rolling around.
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21302697 - 02/20/15 08:35 AM (9 years, 1 month ago) |
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Quote:
van der griegen said:
A little trick you can do is to use the tip of your hot needle to burn a little hole into the agar. That gives the drop a place to sit without rolling around.
Smart idea..I will do that tonight. Thanks for the tip
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blazedup
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Re: first attempt with agar [Re: blazedup]
#21307033 - 02/21/15 07:18 AM (9 years, 1 month ago) |
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21307036 - 02/21/15 07:19 AM (9 years, 1 month ago) |
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 ,
What's that dot in the middle?
--------------------
Gyo's Better Grows
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Dominus fortunae meae sum
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21307042 - 02/21/15 07:22 AM (9 years, 1 month ago) |
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Quote:
taGyo said:
,
What's that dot in the middle?
Lol I thought you might be able to tell me or someone. It looks like a little volcano. There is no discoloration if you see it up close. Really thick growing white in all of it
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21307045 - 02/21/15 07:22 AM (9 years, 1 month ago) |
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Does it look like mycelium? At this point in the game you should be able to tell if it doesn't. There are some white molds that look like what you've got there.
--------------------
Gyo's Better Grows
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blazedup
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Re: first attempt with agar [Re: taGyo]
#21307058 - 02/21/15 07:26 AM (9 years, 1 month ago) |
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Quote:
taGyo said:
Does it look like mycelium? At this point in the game you should be able to tell if it doesn't. There are some white molds that look like what you've got there.
To me it looks like healthy mycelium. It's just hasn't got any bigger yet.
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21307067 - 02/21/15 07:27 AM (9 years, 1 month ago) |
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--------------------
Gyo's Better Grows
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Dominus fortunae meae sum
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blazedup
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Re: first attempt with agar [Re: taGyo]
#21312003 - 02/22/15 08:39 AM (9 years, 1 month ago) |
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Another closer picture of this strange type of growth. I have seen different type of growth through pictures and my own experiments.
 
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21312237 - 02/22/15 10:12 AM (9 years, 1 month ago) |
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What species are you trying to grow?
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21312247 - 02/22/15 10:15 AM (9 years, 1 month ago) |
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Quote:
van der griegen said:
What species are you trying to grow?
I believe that is KSSS
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21312264 - 02/22/15 10:19 AM (9 years, 1 month ago) |
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Looks like mold to me. Did you inoculate there or elsewhere on the plate?
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21312266 - 02/22/15 10:20 AM (9 years, 1 month ago) |
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Doesn't look like any KSSS myc I've ever seen.
And I've seen a lot.
--------------------
Gyo's Better Grows
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blazedup
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Re: first attempt with agar [Re: taGyo]
#21312279 - 02/22/15 10:22 AM (9 years, 1 month ago) |
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I scraped across from another dish. But I don't remember directly touching that spot. Maybe you are right that its mold then.
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blazedup
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Re: first attempt with agar [Re: blazedup]
#21312287 - 02/22/15 10:24 AM (9 years, 1 month ago) |
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Guess I will go clean out that container and start over.
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21312888 - 02/22/15 12:42 PM (9 years, 1 month ago) |
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I'd give it two more days, personally. Your mycelium might show up.
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blazedup
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Re: first attempt with agar [Re: Psilicon]
#21312980 - 02/22/15 01:07 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
I'd give it two more days, personally. Your mycelium might show up.
Just did some transfers today. I was curious when taking a wedge out do you place it on the new agar face down? Because when I take it the agar is extra thick and not all mycelium
Edited by blazedup (02/22/15 01:14 PM)
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21313347 - 02/22/15 02:12 PM (9 years, 1 month ago) |
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However I can get it to stick to the dish is good for me.
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taGyo
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Re: first attempt with agar [Re: Psilicon]
#21316175 - 02/23/15 06:38 AM (9 years, 1 month ago) |
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I try to grab it on the edge of my scalpel so I can gently lay it down on the agar and pull the scalpel out and drop it.
--------------------
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blazedup
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Re: first attempt with agar [Re: taGyo]
#21316336 - 02/23/15 07:52 AM (9 years, 1 month ago) |
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Quote:
taGyo said:
I try to grab it on the edge of my scalpel so I can gently lay it down on the agar and pull the scalpel out and drop it.
I will keep practicing and see what works best.
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NumeroEno
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Re: first attempt with agar [Re: blazedup]
#21316610 - 02/23/15 09:16 AM (9 years, 1 month ago) |
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I use #23 scalpel blades which have a nice sharp point and a round #4 handle. The sharp point helps to easily pick up and drop transfers. This handle was about 7 bucks and so was the 100 pack of pre sterilized blades.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
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Re: first attempt with agar [Re: NumeroEno]
#21316694 - 02/23/15 09:40 AM (9 years, 1 month ago) |
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Quote:
NumeroEno said:
I use #23 scalpel blades which have a nice sharp point and a round #4 handle. The sharp point helps to easily pick up and drop transfers. This handle was about 7 bucks and so was the 100 pack of pre sterilized blades.
I may get myself a scalpel set. I been using an exact-o knife
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NumeroEno
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Re: first attempt with agar [Re: blazedup]
#21316709 - 02/23/15 09:46 AM (9 years, 1 month ago) |
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My favorite thing about scalpels is the pre sterilized blade. The one pictured is the third handle I've bought. I really prefer the round #4 handles over the smaller #3s, and the #23 blade shape is really ideal for agar.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
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Re: first attempt with agar [Re: NumeroEno]
#21316736 - 02/23/15 09:53 AM (9 years, 1 month ago) |
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Quote:
NumeroEno said:
My favorite thing about scalpels is the pre sterilized blade. The one pictured is the third handle I've bought. I really prefer the round #4 handles over the smaller #3s, and the #23 blade shape is really ideal for agar.
Being that they are pre-sterilized do you not sterilize them with a flame after you use it? Or do u toss it and use a new blade each time?
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21316905 - 02/23/15 10:26 AM (9 years, 1 month ago) |
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Flame sterilize before cutting no matter what.
Between you putting the blade on and shit you've lost sterility.
--------------------
Gyo's Better Grows
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Dominus fortunae meae sum
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NumeroEno
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Re: first attempt with agar [Re: taGyo]
#21316924 - 02/23/15 10:29 AM (9 years, 1 month ago) |
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Quote:
taGyo said:
Flame sterilize before cutting no matter what.
Between you putting the blade on and shit you've lost sterility.
I don't touch the blade. I peel the package open slightly and hold it by the package. It works very well.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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taGyo
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Re: first attempt with agar [Re: NumeroEno]
#21316936 - 02/23/15 10:32 AM (9 years, 1 month ago) |
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I use the same lol,
I flame no matter what. I don't trust my air. I've had a few ride along in a jar and I got trich.
--------------------
Gyo's Better Grows
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21316987 - 02/23/15 10:41 AM (9 years, 1 month ago) |
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Better to be safe than sorry
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21319213 - 02/23/15 06:25 PM (9 years, 1 month ago) |
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Quote:
blazedup said:
Another closer picture of this strange type of growth. I have seen different type of growth through pictures and my own experiments.
So I decided to toss it because another little spot of dark bacteria started to grow on the opposite side. I didn't want to take the chance of spreading anything to all my other plates/containers.
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21319215 - 02/23/15 06:26 PM (9 years, 1 month ago) |
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--------------------
Gyo's Better Grows
TNF Q&A
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Dominus fortunae meae sum
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blazedup
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Re: first attempt with agar [Re: taGyo]
#21322736 - 02/24/15 01:23 PM (9 years, 1 month ago) |
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Update .... Wedge transfer completed on 2/22:
     
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PNWMusicMaker
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Re: first attempt with agar [Re: blazedup]
#21322838 - 02/24/15 01:51 PM (9 years, 1 month ago) |
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Right on. Agar is so great.
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Live to change, change to live.
   
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blazedup
Dirk Diggler
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Quote:
PNWMusicMaker said:
Right on. Agar is so great.
your are definitely right. I didn't think it would be this cool.
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PNWMusicMaker
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Re: first attempt with agar [Re: blazedup]
#21323111 - 02/24/15 02:57 PM (9 years, 1 month ago) |
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Agar gets results. I just hate waiting on jars to colonize.
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Live to change, change to live.
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blazedup
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It's going to take some time to understand the results for me. Some stuff it's obvious but others not so much. This is definitely a patient persons hobby. The end result is worth every min, hour, day, week, etc...
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21323140 - 02/24/15 03:04 PM (9 years, 1 month ago) |
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Quote:
blazedup said:
It's going to take some time to understand the results for me. Some stuff it's obvious but others not so much. This is definitely a patient persons hobby. The end result is worth every min, hour, day, week, etc...
Nah! Just start another project. Then another, and then it'll be time to do something with your first one.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21323186 - 02/24/15 03:14 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Quote:
blazedup said:
It's going to take some time to understand the results for me. Some stuff it's obvious but others not so much. This is definitely a patient persons hobby. The end result is worth every min, hour, day, week, etc...
Nah! Just start another project. Then another, and then it'll be time to do something with your first one.
Funny I been doing that and I'm running out of free space lol
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21323241 - 02/24/15 03:28 PM (9 years, 1 month ago) |
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 Almost there, buddy!
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21323310 - 02/24/15 03:40 PM (9 years, 1 month ago) |
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I think I'm going to start packing up some wedges and offer for trades
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21323644 - 02/24/15 04:51 PM (9 years, 1 month ago) |
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Remember that it's very illegal to do this with active species.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21323816 - 02/24/15 05:21 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Remember that it's very illegal to do this with active species.
Not with the ksss...I was referring to pleurotus eryngii
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21323843 - 02/24/15 05:26 PM (9 years, 1 month ago) |
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21323857 - 02/24/15 05:28 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
How much of a wedge would be needed to offer someone a fair trade?
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Grey
⇜ ✯ ⇝
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Re: first attempt with agar [Re: Psilicon]
#21324081 - 02/24/15 06:00 PM (9 years, 1 month ago) |
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I'll trade you something for a wedge of pleurotus
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21324094 - 02/24/15 06:01 PM (9 years, 1 month ago) |
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Quote:
blazedup said:
Quote:
van der griegen said:
How much of a wedge would be needed to offer someone a fair trade?
Enough that they can easily pick it up with a scalpel or exacto.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21324232 - 02/24/15 06:21 PM (9 years, 1 month ago) |
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Quote:
ingclassy said:
I'll trade you something for a wedge of pleurotus
Pm me
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21324242 - 02/24/15 06:22 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Quote:
blazedup said:
Quote:
van der griegen said:
How much of a wedge would be needed to offer someone a fair trade?
Enough that they can easily pick it up with a scalpel or exacto.
Cool ..good info to know. So I should be able to make a few tubes.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21329667 - 02/25/15 06:18 PM (9 years, 1 month ago) |
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So I thought I saw something bad and unfortunately I did. I took a wedge when this thing looked healthy and now it turned on me.
 
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21329724 - 02/25/15 06:33 PM (9 years, 1 month ago) |
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I can't see what's going on there. It's too blurry. Try putting it down.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21329751 - 02/25/15 06:40 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
I can't see what's going on there. It's too blurry. Try putting it down.
That was one of the best pictures but I noticed when u select it the zoom makes it blurry. I can't take anymore because I tossed it. The color was like a dark green/blue color.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21334392 - 02/26/15 06:06 PM (9 years, 1 month ago) |
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New pics ..... This time I think it's no bueno.
   
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21334405 - 02/26/15 06:08 PM (9 years, 1 month ago) |
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Yep. Looks pretty skanky to me, too.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21334422 - 02/26/15 06:11 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Yep. Looks pretty skanky to me, too.
Time to clean them out and start over. Do u think this is the type of contam that needs special treatment disposing? Also it was Ina drawer with other agar growing, can it possibly spread?
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21334436 - 02/26/15 06:13 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Yep. Looks pretty skanky to me, too.
Also the white growth in the 3rd and 4th picture myc growth? Can it be saved?
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taGyo
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Re: first attempt with agar [Re: blazedup]
#21334460 - 02/26/15 06:17 PM (9 years, 1 month ago) |
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In theory yes but I wouldn't do it because sometimes myc looks good but has ride along contams.
--------------------
Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21334467 - 02/26/15 06:19 PM (9 years, 1 month ago) |
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Quote:
taGyo said:
In theory yes but I wouldn't do it because sometimes myc looks good but has ride along contams.
True, good point. I'm worried whatever this contamination is going to spread to stuff it's been around
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taGyo
Strainiac/AMU
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Re: first attempt with agar [Re: blazedup]
#21334487 - 02/26/15 06:21 PM (9 years, 1 month ago) |
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Nah, just toss and clean with soap and water. Toss outside. I throw all of my myc into a hole under a big tree in my backyard. My downstairs neighbor is gonna be pissed but if it works I wont be.
--------------------
Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: taGyo]
#21334498 - 02/26/15 06:24 PM (9 years, 1 month ago) |
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Quote:
taGyo said:
Nah, just toss and clean with soap and water. Toss outside. I throw all of my myc into a hole under a big tree in my backyard. My downstairs neighbor is gonna be pissed but if it works I wont be.
Lol
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21334562 - 02/26/15 06:35 PM (9 years, 1 month ago) |
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Nope! Don't worry about it. Just put it out and into a plastic bag or something and then throw it in your outside trash receptacle.
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NumeroEno
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Re: first attempt with agar [Re: Psilicon]
#21337432 - 02/27/15 09:35 AM (9 years, 1 month ago) |
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Now that you've played around with agar a bit you should think about getting into pouring plates. I was very daunted by it at first but now it's easy. I use this bottle for pouring plates.
Just order some petris and some parafilm. You already have everything else. The major advantage with poured plates is that you can see the surface of the agar really well, provided you pour at the right temp to mitigate condensation.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: NumeroEno]
#21337597 - 02/27/15 10:15 AM (9 years, 1 month ago) |
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Quote:
NumeroEno said:
Now that you've played around with agar a bit you should think about getting into pouring plates. I was very daunted by it at first but now it's easy. I use this bottle for pouring plates.
Just order some petris and some parafilm. You already have everything else. The major advantage with poured plates is that you can see the surface of the agar really well, provided you pour at the right temp to mitigate condensation.
I actually i already have petri just have to finally pour dishes
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NumeroEno
I come from the land of lizards
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Posts: 9,652
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Re: first attempt with agar [Re: blazedup]
#21337634 - 02/27/15 10:24 AM (9 years, 1 month ago) |
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Nice! It's worth it 
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: NumeroEno]
#21338661 - 02/27/15 02:10 PM (9 years, 1 month ago) |
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Quote:
NumeroEno said:
Now that you've played around with agar a bit you should think about getting into pouring plates. I was very daunted by it at first but now it's easy. I use this bottle for pouring plates.
Just order some petris and some parafilm. You already have everything else. The major advantage with poured plates is that you can see the surface of the agar really well, provided you pour at the right temp to mitigate condensation.
The one thing I don't have is para film. Is it truly necessary?
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taGyo
Strainiac/AMU
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Re: first attempt with agar [Re: blazedup]
#21338689 - 02/27/15 02:17 PM (9 years, 1 month ago) |
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No,
Use glad cling wrap. Cling wrap specifically because its permeable. Other wraps may not work. Cut it down to size and wrap an empty Petri to practice. Strips 2" are best.
--------------------
Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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NumeroEno
I come from the land of lizards
Registered: 07/24/14
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Re: first attempt with agar [Re: taGyo]
#21338872 - 02/27/15 03:01 PM (9 years, 1 month ago) |
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Cling wrap will work, but parafilm fucking rocks.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: NumeroEno]
#21347269 - 03/01/15 09:05 AM (9 years, 1 month ago) |
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New pics: wedge transfer from 2/22
   
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: blazedup]
#21351389 - 03/02/15 10:33 AM (9 years, 1 month ago) |
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Another one bites the dust. What causes the little balls of liquid?
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21352915 - 03/02/15 05:25 PM (9 years, 1 month ago) |
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Sometimes when mycelium is battling a host of bacteria, it'll start putting out metabolites which include various antibiotics. These are water soluble, but they can't go anywhere unless water is also excreted, so you often see water droplets or puddling in bacterial grows.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21352972 - 03/02/15 05:41 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Sometimes when mycelium is battling a host of bacteria, it'll start putting out metabolites which include various antibiotics. These are water soluble, but they can't go anywhere unless water is also excreted, so you often see water droplets or puddling in bacterial grows.
So anytime there are metabolizes that would men there is bacteria present?
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Psilicon
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Re: first attempt with agar [Re: blazedup]
#21353194 - 03/02/15 06:34 PM (9 years, 1 month ago) |
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They excrete metabolites for other reasons, like digestion or heat stress. But I'd say it's fair to say that every time you see bacteria, metabolites are present. So if your culture is on something easy to digest like an agar media and if the heat has been stable and within range, metabolites are a sure sign of bacteria. But in this case the bacteria is pretty easy to spot without that sign.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21367550 - 03/05/15 06:08 PM (9 years, 1 month ago) |
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So I did a transfer and I noticed this white stuff starting to spread. I assume its contamination. Sorry for the crappy picture but I didn't want to open it.
 
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Psilicon
Really Nice Guy
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Re: first attempt with agar [Re: blazedup]
#21367747 - 03/05/15 06:57 PM (9 years, 1 month ago) |
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Different bacteria. =(
I think at this point we need to look at your SAB.
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blazedup
Dirk Diggler
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Re: first attempt with agar [Re: Psilicon]
#21367789 - 03/05/15 07:06 PM (9 years, 1 month ago) |
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Quote:
van der griegen said:
Different bacteria. =(
I think at this point we need to look at your SAB.
Yea that was my thought. I need to make a bleach mix and give it a super cleaning and start reevaluating my sterile process.
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