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 trippyshroom
Bacterial genius
 
Registered: 05/07/13
Posts: 363
Last seen: 6 months, 7 days
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 Re: first attempt with agar  [Re: mushpunx]
#21271277 - 02/13/15 11:27 PM (9 years, 2 months ago) |
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I absolutely love agar I'm still a total noob at it but right now I got some Cambodian starting on agar and PE and a Brazilian clone.
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Anything that is posted above is just a figment of your imagination.
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Sorry for the poor pictures but my phone couldn't seem to focus correctly and I didn't want to chance contams from having it open for more then a couple seconds.
   
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land 
Last seen: 5 years, 5 months
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Re: first attempt with agar [Re: blazedup]
#21273040 - 02/14/15 12:13 PM (9 years, 2 months ago) |
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Doesn't look all good to me, you need better pics though.
How many drops did you do? Did you move the plate directly after dropping it, causing the drop to move around and spread spores everywhere?
You're looking for pure white:
The milky stuff is bacteria:
This is cubensis:
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Gyo's Better Grows
TNF Q&A 
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Dominus fortunae meae sum
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Re: first attempt with agar [Re: taGyo]
#21273070 - 02/14/15 12:20 PM (9 years, 2 months ago) |
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Quote:
taGyo said:
Doesn't look all good to me, you need better pics though.
How many drops did you do? Did you move the plate directly after dropping it, causing the drop to move around and spread spores everywhere?
You're looking for pure white:
The milky stuff is bacteria:
This is cubensis:
Unfortunately I ended up putting more then a drip in there and when putting it away it spread around because too much liquid
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Re: first attempt with agar [Re: blazedup]
#21273076 - 02/14/15 12:21 PM (9 years, 2 months ago) |
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Ahh,
Watch it then. Transfer myc at the first signs of healthy growth.
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Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Psilicon
Really Nice Guy
Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
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Re: first attempt with agar [Re: taGyo]
#21273077 - 02/14/15 12:22 PM (9 years, 2 months ago) |
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I think the biggest blotch, which he seems to be centered on, looks okay. I'd transfer from there now. I agree with tag that it looks like it spread around, though.
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Re: first attempt with agar [Re: Psilicon]
#21273088 - 02/14/15 12:26 PM (9 years, 2 months ago) |
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Ok I'll make some new dishes ASAP...and make a loop to transfer. Does it make sense to cut the piece out to transfer or just scrape the top?
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Psilicon
Really Nice Guy
Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
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Re: first attempt with agar [Re: blazedup]
#21273106 - 02/14/15 12:31 PM (9 years, 2 months ago) |
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I've heard scraping works from people I respect, but haven't tried it so I can't recommend it personally.
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spacechildo
proletarians rise up
Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 7 months
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Re: first attempt with agar [Re: Psilicon]
#21273481 - 02/14/15 02:23 PM (9 years, 2 months ago) |
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I wouldn't recommend it but I've done it when the leading edges look bacterial,
just lightly touch an area where the cube myc has "risen" a bit above the plate
and touch it again on your new plate.
I've only done it a handful times but still considered every success to be "luck".
You should try it vis a vis the "nothing to lose" theorY!
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Quote:
spacechildo said:
I wouldn't recommend it but I've done it when the leading edges look bacterial,
just lightly touch an area where the cube myc has "risen" a bit above the plate
and touch it again on your new plate.
I've only done it a handful times but still considered every success to be "luck".
You should try it vis a vis the "nothing to lose" theorY!
You don't recommend which option?
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spacechildo
proletarians rise up
Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 7 months
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Re: first attempt with agar [Re: blazedup]
#21273540 - 02/14/15 02:38 PM (9 years, 2 months ago) |
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scraping the top of clean looking myc. I've done it with success a handful times but each time it has felt like luck.
Dont ask me how luck feels, just that I never expected it to work but yet it somehow did 
I'd rather start over, but as I stated, what have you got to lose? 
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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So I did the transfer using the scrape method...after getting a better look in the jar there was a nice growth going on. The second one I opened looked like straight bacteria because it was cloudy white spots and just didn't look right. I also did my first print to agar transfer.
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 Grey
⇜ ✯ ⇝
Registered: 11/06/14
Posts: 6,223
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Re: first attempt with agar [Re: blazedup]
#21276857 - 02/15/15 11:00 AM (9 years, 2 months ago) |
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I did print to agar. The first ones took 7-8 days to show any signs of growth. I made up some more plates, but cut back .5g on the agar giving it a more "preserve" look rather than a stiff wax. I used some of the same spores I used on the first plates on the new ones, as well as some GT and Texan that was printed last month. I'm hoping the more aqueous agar and fresher spores will take off sooner. That and I used a scalpel instead of a sterile swab so I got a ton of spores put down.
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  AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Re: first attempt with agar [Re: Grey]
#21276883 - 02/15/15 11:05 AM (9 years, 2 months ago) |
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Quote:
ingclassy said:
I did print to agar. The first ones took 7-8 days to show any signs of growth. I made up some more plates, but cut back .5g on the agar giving it a more "preserve" look rather than a stiff wax. I used some of the same spores I used on the first plates on the new ones, as well as some GT and Texan that was printed last month. I'm hoping the more aqueous agar and fresher spores will take off sooner. That and I used a scalpel instead of a sterile swab so I got a ton of spores put down.
Nice work..any pics? I want to figure out a better agar mix because the potato agar seems to come out very thick and the color ain't the greatest. I prefer something more transparent
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Grey
⇜ ✯ ⇝
Registered: 11/06/14
Posts: 6,223
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Re: first attempt with agar [Re: blazedup]
#21276953 - 02/15/15 11:15 AM (9 years, 2 months ago) |
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I cant get them to upload. I'm switching phones today, maybe my new one won't suck. I made a double batch of pasty plates so the mix, with reduces agar, ended up being:
1 cup water,
6.6g potato flakes,
4.5 grams agar powder (would have been 5 if I had used the same amount as the first batch), and 3.1g honey.
I used food coloring but only three drops so it came out almost pink. Everyone says the mycelium eats up the red so I used that to kinda measure growth from the bottom.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Grey
⇜ ✯ ⇝
Registered: 11/06/14
Posts: 6,223
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Re: first attempt with agar [Re: Grey]
#21277555 - 02/15/15 01:08 PM (9 years, 2 months ago) |
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Here we go:
   
You can't see that well, but I can tell where I rolled the swab because spores germinated in different spots. It took a while to germinate, but they're almost doubling in size every day now.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
Edited by Grey (02/15/15 01:14 PM)
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Re: first attempt with agar [Re: Grey]
#21277569 - 02/15/15 01:10 PM (9 years, 2 months ago) |
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Three looks like a little bacteria in there. Two and four look fine, I think one might have a little bacteria as well.
Agar Bacteria:
Bacteria is kind of milky, whiteish. Looks like a pool of milky white water.
--------------------
Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
Edited by taGyo (02/15/15 01:13 PM)
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Re: first attempt with agar [Re: Grey]
#21277586 - 02/15/15 01:12 PM (9 years, 2 months ago) |
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Quote:
ingclassy said:
Here we go:
Thanks for posting the pics
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blazedup
Dirk Diggler
Registered: 06/05/11
Posts: 1,235
Loc: Middle Earth
Last seen: 9 days, 5 hours
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Re: first attempt with agar [Re: blazedup]
#21277591 - 02/15/15 01:13 PM (9 years, 2 months ago) |
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I'm starting to really like agar
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Psilicon
Really Nice Guy
Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
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Re: first attempt with agar [Re: blazedup]
#21277604 - 02/15/15 01:14 PM (9 years, 2 months ago) |
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Quote:
taGyo said:
Bacteria is kind of milky, whiteish. Looks like a pool of milky white water.
Yeah, or the plaque you scrape off your teeth. 
Quote:
blazedup said:
I'm starting to really like agar
Good! I knew you would. 
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