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 Pastywhyte 
Say hello to my little friend
 
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Quote:
elasticaltiger said:
Quote:
blindingleaf said:
i like how u keep them upside down man, thats a great idea!!
I haven't read the entire pasty thread, was this mentioned in there a long time ago or something?
Not in the OP, I never really went into procedure at all. That's why I love when people do stuff like this. Both this thread and the Tiger Drop are linked in the OP 
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 10,128
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 Re: SAB Sterile Tek Video Series  [Re: Pastywhyte]
#21033775 - 12/27/14 11:57 PM (9 years, 3 months ago) |
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Nice videos man. I'm happy to know that I do most of the stuff you're doing in there the same way. I don't put my plate upside down though, although I do open them upside down in my hands when looking for stuff.
A few questions:
1. I find that having the spore syringe so high when letting the droplet go often creates a splash in there. It makes it look like I have satellites or promotes weird growth. Do you get this problem?
2. Why don't you cool your blade off in the receiving agar plate? Wouldn't the hot blade damage the mycelium you're cutting into or is that just some overly careful BS people spew?
Thanks!
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wowimflabbergasted
supercalifragilistic
Registered: 07/16/12
Posts: 18,919
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 Great work!
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Quote:
stareatclouds said:
Nice videos man. I'm happy to know that I do most of the stuff you're doing in there the same way. I don't put my plate upside down though, although I do open them upside down in my hands when looking for stuff.
A few questions:
1. I find that having the spore syringe so high when letting the droplet go often creates a splash in there. It makes it look like I have satellites or promotes weird growth. Do you get this problem?
2. Why don't you cool your blade off in the receiving agar plate? Wouldn't the hot blade damage the mycelium you're cutting into or is that just some overly careful BS people spew?
Thanks!
1. lets find out.
nope
2.I use an x-acto blade. Its super thin and cools off pretty quick. I'd never bothered to try to cool it, and never had any problems. I'd imagine the fixed blade scalpel you see in RR's vids has a bit more body to it so it might cool down slower than my little disposable blade.
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 10,128
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21034662 - 12/28/14 09:59 AM (9 years, 3 months ago) |
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Hi Munch,
I actually don't think you can eyeball the splashes I'm talking about. They're very tiny and show up when the mycelium starts developing. But I guess you would've noticed this beforehand as I'm sure you've done many single drops from that high up.
As far as blade cooling, I also use an X-acto blade and it takes me 3 dips in the receiving plate before it stops making the "tssss" burning sound. But I'm sure it's not that big of a deal.
Thanks again for the videos!
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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in the agar to agar video it looks like you may have put your left hand on the receiving dishes' lid, hard to tell from the angle but it may be behind the lid too. hands never above sterile surfaces.
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Quote:
stareatclouds said:
I actually don't think you can eyeball the splashes I'm talking about. They're very tiny and show up when the mycelium starts developing.
As far as blade cooling, I also use an X-acto blade and it takes me 3 dips in the receiving plate before it stops making the "tssss" burning sound. But I'm sure it's not that big of a deal.
like dis?
I've only ever had this happen when I get too much on the plate, like more than one drop, which is easy to do. Not all my syringes flow as smoothly as the APE used in the video. But when I do the single drop, they all germ nicely in the middle. Is it possible you might have tilted the plate and shifted the water?
I just heated my #2 xacto blade and it was cool to the touch after a 20 count. Here is a video showing that my blade has cooled to the touch by the time it hits the agar in my video.
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 10,128
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21034799 - 12/28/14 10:37 AM (9 years, 3 months ago) |
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Yep, like that, and I'm sure it's possible I tilted it. I'll do some more with single drops soon. I also use my X-acto immediately after it's flame sterilized as I thought it was supposed to be used ASAP after red hot.
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: SAB Sterile Tek Video Series [Re: bodhisatta] 1
#21034812 - 12/28/14 10:38 AM (9 years, 3 months ago) |
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Quote:
bodhisatta said:
in the agar to agar video it looks like you may have put your left hand on the receiving dishes' lid, hard to tell from the angle but it may be behind the lid too. hands never above sterile surfaces.
oh shit you are right. good eyes. I probably didn't notice it when it happened because I was watching myself on the video screen to make sure I was in frame, rather than concentrate on my hand placement. I will have the video replaced later today.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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nice work though
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Quote:
stareatclouds said:
I also use my X-acto immediately after it's flame sterilized as I thought it was supposed to be used ASAP after red hot.
I usually do too, but just the process of turning my torch off, putting my hands in the sab, and then grabbing the plate all takes roughly 15-20 seconds, so its cooled down by then. I'm not doing any active waiting for it to cool down. RR drags it through agar because he has a much more convenient setup with his flowhood, and he doesn't have any time between flaming and working like I do with the SAB.
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21034895 - 12/28/14 10:57 AM (9 years, 3 months ago) |
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You turn the torch off? Shit I just leave it going usually.
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: SAB Sterile Tek Video Series [Re: Pastywhyte]
#21034914 - 12/28/14 11:01 AM (9 years, 3 months ago) |
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just for safety reasons and because I can do multiple plates with the syringe since there is no contact. I get scared that I might knock it off my table and then it catches my house on fire. I get cases of power 5x for $20, so its def not me being cheap. PF-tek, I leave it on and do 5 jars at a time, turn the torch off and take a breather and do a dab or something. I fucking hate pf jars.
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 10,128
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21034936 - 12/28/14 11:05 AM (9 years, 3 months ago) |
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Quote:
and he doesn't have any time between flaming and working like I do with the SAB.
I work in a SAB and have a standard alcohol flame and it takes me like 2 seconds to move the blade from flame to agar plate.
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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 cool it of you need to. I don't always go straight on to the plate, it really depends on the tool and tek I'm using.
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21037032 - 12/28/14 08:23 PM (9 years, 3 months ago) |
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NumeroEno
I come from the land of lizards
Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge 
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21037039 - 12/28/14 08:25 PM (9 years, 3 months ago) |
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Gonna have to check these videos out on my computer.
--------------------
  Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in DOG FOOD AGAR MY ELECTRIC INOCULATION LOOP
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21037041 - 12/28/14 08:26 PM (9 years, 3 months ago) |
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What's up with the tape? 
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: SAB Sterile Tek Video Series [Re: Inocuole]
#21037050 - 12/28/14 08:28 PM (9 years, 3 months ago) |
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can't be too careful
clean long sleeves and tape, next best thing to tyvek sleeves when I'm out. I figured most people probably don't have them, though.
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: SAB Sterile Tek Video Series [Re: Munchauzen]
#21037055 - 12/28/14 08:29 PM (9 years, 3 months ago) |
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Ah okay. I kinda hate the tyvek sleeves sometimes when I use them. I've taken to just using my elbow length gloves and trying not to get my whole arm in there most of the time unless it's super serious.
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