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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: mushpunx]
    #20994855 - 12/18/14 03:51 PM (9 years, 3 months ago)

Bro,

That's what I'm saying :lol:. You really have to run an agar-plate through the air or do some experiments to realize that your environment is DIRTY but clean enough for YOU to live in, you know what I mean?

All this open-air stuff gives me a headache when you could buy a hole saw for 10-20 bucks or heat up a knife or a soup can for gods sake.


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InvisibleMrGiraffe

Registered: 04/04/14
Posts: 3,149
Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitation [Re: taGyo]
    #20995136 - 12/18/14 04:51 PM (9 years, 3 months ago)

I used to open air G2G in a tiny ass closet with a wood floor.  I'd load it with my stuff, and spray the shit out of it with lysol with a towel stuffed under the door,  Then I'd shower, mask up, spray the shit out of again as I crept in.  It was like a human sized SAB.  I actually only ended up with one contam out of the times I did it, and that was from a bad syringe.  The original grain jars nocced with it all contaminated but one, and that last one eventually failed after the G2G. 
Now disclaimer before people jump on my shit, I eventually built a small flow hood and stopped playing the lottery and there's no way in hell I'd tell any one to attempt open air any thing.  I fucking love my flow hood lol.


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InvisibleSpitballJedi
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20995151 - 12/18/14 04:54 PM (9 years, 3 months ago)

Excellent post taGyo. 5 shrooms for you


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #20995247 - 12/18/14 05:17 PM (9 years, 3 months ago)

Quote:

SpitballJedi said:
Excellent post taGyo. 5 shrooms for you



:bow:

Means a lot SBJ. Thanks for stopping by,

:super:


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Invisibleblackdust
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20997914 - 12/19/14 07:26 AM (9 years, 3 months ago)

Quote:

taGyo said:
I've been seeing a lot of threads and people talking about open-air g2g.

These are the problems you could encounter without knowing. I ran an open-air experiment by running an agar plate through the air.



The other one I breathed on. The breathing one got a shit ton of bacteria but nothing special. The open-air one, however, got Asperigilus.



I also alcohol sanitized my hands and touched an agar plate with my index finger:



Unidentified as of now.

Moral Of The Story:

Proper Sterile Technique Is Key!

Open-Air G2G is a bad idea, I live in a clean house and still got this. Alcohol does not replace flame sterilization.


Hopefully this thread will help someone from doing something stupid.





make an agar plate and let it sit without opening. Let me know how long before it contams.

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: blackdust]
    #20997922 - 12/19/14 07:29 AM (9 years, 3 months ago)

I've actually done that,

It didn't :lol:, I used to use the same plate to cool my scalpel off because I was afraid of contams on my receiving dish. I'd literally use that dish over and over and over and over and over.

Then I realized I was being an idiot.


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Offlinetripdawg420
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20998009 - 12/19/14 08:14 AM (9 years, 3 months ago)

:thumbup:


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OfflinePsyCLown89
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: tripdawg420]
    #20998586 - 12/19/14 11:43 AM (9 years, 3 months ago)

Only saw the thread now, awesome thread indeed! :laugh:


BTW noticed quite a bit of supporter talk going on - drop geokills or Asante a PM about supporter accounts.


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: PsyCLown89]
    #20998590 - 12/19/14 11:44 AM (9 years, 3 months ago)

:eek:,

I will right now.


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Offlinemushpunx
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20998608 - 12/19/14 11:50 AM (9 years, 3 months ago)

What's a supporter account?


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InvisibleMunchauzen
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: mushpunx]
    #20998623 - 12/19/14 11:55 AM (9 years, 3 months ago)

unlimited storage, color gradient name, larger avatar, faster colonization

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Munchauzen]
    #20998633 - 12/19/14 11:57 AM (9 years, 3 months ago)

Quote:

Munchauzen said:
faster colonization



:solidnod:

:canthelpbutlaugh:


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InvisibleSpitballJedi
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #20999249 - 12/19/14 02:58 PM (9 years, 3 months ago)

:dawerp:


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Offlinemushpunx
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Munchauzen]
    #20999270 - 12/19/14 03:04 PM (9 years, 3 months ago)

Quote:

Munchauzen said:
unlimited storage, color gradient name, larger avatar, faster colonization





Ahahahahaha


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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Munchauzen]
    #21208285 - 01/31/15 06:57 PM (9 years, 1 month ago)

Quote:

Munchauzen said:
unlimited storage, color gradient name, larger avatar, faster colonization




:havesomescience:


--------------------
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InvisibleSpitballJedi
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21250514 - 02/09/15 07:59 PM (9 years, 1 month ago)

Quote:

taGyo said:
Hopefully this thread will help someone from doing something stupid.





:lol:

If you can just save one....


Nice post dude.


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A little civility goes a long way

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OfflinetaGyo
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21250523 - 02/09/15 08:00 PM (9 years, 1 month ago)

This was my last open-air experiment:


Thanks SBJ,
Figured I should put it in my sig :lol:


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InvisibleSpitballJedi
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: taGyo]
    #21250721 - 02/09/15 08:34 PM (9 years, 1 month ago)

Looks like pasta wheels and cream sauce. :lol:


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InvisibleFuzz-nutter
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: SpitballJedi]
    #21250877 - 02/09/15 09:08 PM (9 years, 1 month ago)

Can I give you 5 shrooms for the worst hand-writing I've ever seen?
:tongue2:

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OfflineKizzle
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Re: Open Air G2G/Flame Sterilization versus Alcohol Sanitization [Re: Fuzz-nutter]
    #21250988 - 02/09/15 09:37 PM (9 years, 1 month ago)

Quote:


Bro,

That's what I'm saying :lol:. You really have to run an agar-plate through the air or do some experiments to realize that your environment is DIRTY but clean enough for YOU to live in, you know what I mean?

All this open-air stuff gives me a headache when you could buy a hole saw for 10-20 bucks or heat up a knife or a soup can for gods sake.




Of course. The main reason the environment is so dirty in the first place is because you're in it. It's kind of funny the trouble some people have gone through to try to create a sterile room only to put the filthiest thing in the house inside it, a human being :lol:


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