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OfflineDarthGeorge
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Popping/bubbling while flame sterilizing the needle?
    #20854020 - 11/18/14 01:32 AM (9 years, 4 months ago)

I'm using a BIC lighter to flame sterilize the needle. After inoculating a jar, I pull it out to flame it and it pops and bubbles. Do I have it to close to the needle? Or is this normal?

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InvisibleInocuole
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Re: Popping/bubbling while flame sterilizing the needle? [Re: DarthGeorge]
    #20854023 - 11/18/14 01:33 AM (9 years, 4 months ago)

That's water inside the needle boiling, pretty normal.  Just keep doing it till the tip glows red, and don't let it spurt hot ass water on you.


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OfflineDarthGeorge
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Inocuole]
    #20854032 - 11/18/14 01:36 AM (9 years, 4 months ago)

Ah ok. Thank you.

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OfflinePiranha
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Inocuole]
    #20868462 - 11/20/14 11:16 PM (9 years, 4 months ago)

When sterilizing the needle do you sterilize the whole thing or just the tip. I don't want to waste spores...

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Invisiblewowimflabbergasted
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Piranha]
    #20868469 - 11/20/14 11:18 PM (9 years, 4 months ago)

The tip.


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OfflinePiranha
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Re: Popping/bubbling while flame sterilizing the needle? [Re: wowimflabbergasted]
    #20868475 - 11/20/14 11:22 PM (9 years, 4 months ago)

Can't contams be all along the side of the needle as well?

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InvisibleToxicDuck
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Piranha]
    #20868481 - 11/20/14 11:25 PM (9 years, 4 months ago)

Quote:

Piranha said:
Can't contams be all along the side of the needle as well?





If the tip is glowing red enough heat has transferred to the rest of the needle to be good.

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OfflinePiranha
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Re: Popping/bubbling while flame sterilizing the needle? [Re: ToxicDuck]
    #20868488 - 11/20/14 11:28 PM (9 years, 4 months ago)

Does that mean that all the spore water in the actual needle is all useless? I'm trying to make 4 syringes inoculate 48 half pint jars.

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InvisibleMunchauzen
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Piranha]
    #20868496 - 11/20/14 11:31 PM (9 years, 4 months ago)

yes. its going to boil away.

1cc per jar. how many cc's in your syringes?

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Invisiblewowimflabbergasted
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Piranha]
    #20868500 - 11/20/14 11:32 PM (9 years, 4 months ago)

The amount of spores in the needle are such a small fraction of the whole syringe it doesn't matter.


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OfflinePiranha
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Munchauzen]
    #20868503 - 11/20/14 11:33 PM (9 years, 4 months ago)

Roughly 10 a little more in each. Is that stretching it a little far or is it possible?

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Invisiblewowimflabbergasted
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Piranha]
    #20868508 - 11/20/14 11:35 PM (9 years, 4 months ago)

Anything is possible :lol:


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OfflinePiranha
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Re: Popping/bubbling while flame sterilizing the needle? [Re: wowimflabbergasted]
    #20868518 - 11/20/14 11:40 PM (9 years, 4 months ago)

I was thinking two inoculation holes in each jar and injecting  .4 CCS per injection point... Well maybe more like .3 does this seem unstable in anyway to anyone?

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Invisiblewowimflabbergasted
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Re: Popping/bubbling while flame sterilizing the needle? [Re: Piranha]
    #20868528 - 11/20/14 11:44 PM (9 years, 4 months ago)

2 holes will work, it will just take longer to colonize. And good luck measuring .3ml 48 times lol.


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OfflineNickShade
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Re: Popping/bubbling while flame sterilizing the needle? [Re: wowimflabbergasted]
    #20868548 - 11/20/14 11:50 PM (9 years, 4 months ago)

Just do 4 holes and .25cc per hole. No sense in tampering with a tried and true method and risking unnecessary complication.


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Offlinetombosley8
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Re: Popping/bubbling while flame sterilizing the needle? [Re: NickShade]
    #20868561 - 11/20/14 11:53 PM (9 years, 4 months ago)

better yet try agar and turn 1 drop of ms into a hundred cakes . well you could but that might be pushing it a bit but not much. i just want to make known how valuable agar can be for expanding ms :stoner:  along with endless other benefits.

Edited by tombosley8 (11/21/14 12:00 AM)

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