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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: Aero]
    #20889257 - 11/25/14 03:31 PM (9 years, 4 months ago)

second batch just arrived
agar is cooling, once its solid gonna do 2 jars of each kind

Tampanensis, Mexicana, Mex Pajarito




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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: Aero]
    #20893104 - 11/26/14 01:04 PM (9 years, 4 months ago)

the two mexicana jars are already showing sings of growth :smile:
these are the fastest so far out of the 4 cultures


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Edited by Aero (11/26/14 01:12 PM)

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InvisiblecArcace-x
YeAh!!
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Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
Re: cloning sclerotia [Re: Aero]
    #20893140 - 11/26/14 01:16 PM (9 years, 4 months ago)

:popcorn:


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Invisibleazur
God of Fuck
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: cloning sclerotia [Re: cArcace-x]
    #20896079 - 11/27/14 08:23 AM (9 years, 4 months ago)

Quote:

cArcace-x said:
:popcorn:




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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: cloning sclerotia [Re: Aero]
    #20896573 - 11/27/14 10:55 AM (9 years, 4 months ago)

sweet thread, good luck :mushroom2:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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Invisibleeatyualive
Eat's You Alive :)
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Re: cloning sclerotia [Re: Aero]
    #20896974 - 11/27/14 12:55 PM (9 years, 4 months ago)

Quote:

Aero said:
This is how i make the syringes from colonized agar jars

Once the agar is 100% colonized i make a few syringes with sterile water

As u can see it on the picture the jars i use have metal lids, i put two holes on them.
one is with polyfil the other is with RTV silicone.

the following is happening inside the glovebox:

I squirt some water in the jar then with circular motions the water washes the agar off the wall.

Next step I get a clean brand new ziplock baggie. Open the jar open the baggie, slip the agar into the baggie and close it.

U got some water u got the fully colonized agar wedge ready to rock.
Massage the agar then press it till it falls apart into tiny pieces. some agar will be more difficult to break depends on the mix really.

once its done, get some IPA and spray the side of the bag. wipe it clean then poke a whole with the needle, squirt some more water in then start sucking the water back up. when it stops squirt some in the suck it back again, ull get bigger clumps blocking the needle but with a bit of patient ull get a syringe full of colonized agar pieces floating in the water


ive been using this method for quite some time and never failed me :smile:

hope it helps for some to extend cultures to jars and so on
comes very handy when u are running out of something or just on the last jar, put some colonized grain on agar wait till its colonized then make a few syringes out of it


enjoy :wink:



ah the old bag method! gotta love a ziplock. :thumbup:


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: eatyualive]
    #20897186 - 11/27/14 01:51 PM (9 years, 4 months ago)

Yea man. Never fails me!


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InvisibleToxicDuck
Burt Macklin - F.B.I.
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Registered: 08/25/14
Posts: 1,021
Re: cloning sclerotia [Re: Aero]
    #20897629 - 11/27/14 04:15 PM (9 years, 3 months ago)

Looking forward to more updates.


I also must add that I'm super jealous that you can just order stones :lol:.

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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: ToxicDuck]
    #20897693 - 11/27/14 04:42 PM (9 years, 3 months ago)

gonna soak some rye and shoot the jars with the Atl7 clones


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OfflineAero
Orea


Registered: 11/01/13
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Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: Aero]
    #20905053 - 11/29/14 04:04 PM (9 years, 3 months ago)

growth in all jars made from the second batch of stones :smile:


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Invisibleeatyualive
Eat's You Alive :)
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Registered: 08/17/01
Posts: 19,026
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Re: cloning sclerotia [Re: Aero]
    #20905065 - 11/29/14 04:08 PM (9 years, 3 months ago)

:thumbup:


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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InvisibleToxicDuck
Burt Macklin - F.B.I.
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Registered: 08/25/14
Posts: 1,021
Re: cloning sclerotia [Re: Aero]
    #20907198 - 11/30/14 06:05 AM (9 years, 3 months ago)

Quote:

Aero said:
growth in all jars made from the second batch of stones :smile:





Nice :smile:!

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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: ToxicDuck]
    #20995308 - 12/18/14 05:29 PM (9 years, 3 months ago)



Clone wars

Tampanensis
Mexicana
Mexicana pajarito
Galindoi Atl#7


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Edited by Aero (12/18/14 05:46 PM)

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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
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Re: cloning sclerotia [Re: Aero]
    #20995312 - 12/18/14 05:30 PM (9 years, 3 months ago)

:dawerp:,

Got any extra stone producers you want to send to a fan? :lol:


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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: taGyo]
    #20995387 - 12/18/14 05:45 PM (9 years, 3 months ago)

Next time try pm. This isn't the marketplace really -_-


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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
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Re: cloning sclerotia [Re: Aero]
    #20995399 - 12/18/14 05:48 PM (9 years, 3 months ago)

I didn't even expect a yes :lol:,

Wasn't really a marketplace question anyway :wink:


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Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: taGyo]
    #20995469 - 12/18/14 06:05 PM (9 years, 3 months ago)

If u :pm: u might get lucky


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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: Aero]
    #21035837 - 12/28/14 02:51 PM (9 years, 3 months ago)

9days after inoculation :wink:


The ATL7 the Pajarito already shows sclerotia veins :wink:
lookin good so far, very aggressive colonizers, the mexicana has the lightest mycelium out of the four


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OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
Re: cloning sclerotia [Re: Aero]
    #21035957 - 12/28/14 03:23 PM (9 years, 3 months ago)

ATL7 closeup


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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
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Re: cloning sclerotia [Re: Aero]
    #21035969 - 12/28/14 03:26 PM (9 years, 3 months ago)

:noargument:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum

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