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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: Aero]
#20889257 - 11/25/14 03:31 PM (9 years, 4 months ago) |
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second batch just arrived agar is cooling, once its solid gonna do 2 jars of each kind
Tampanensis, Mexicana, Mex Pajarito
-------------------- SPREAD THE SPORES
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: Aero]
#20893104 - 11/26/14 01:04 PM (9 years, 4 months ago) |
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the two mexicana jars are already showing sings of growth these are the fastest so far out of the 4 cultures
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Edited by Aero (11/26/14 01:12 PM)
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cArcace-x
YeAh!!
Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: cloning sclerotia [Re: Aero]
#20893140 - 11/26/14 01:16 PM (9 years, 4 months ago) |
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azur
God of Fuck
Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: cloning sclerotia [Re: cArcace-x]
#20896079 - 11/27/14 08:23 AM (9 years, 4 months ago) |
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Quote:
cArcace-x said:
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filthyknees
no coincidence
Registered: 03/08/13
Posts: 6,283
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Re: cloning sclerotia [Re: Aero]
#20896573 - 11/27/14 10:55 AM (9 years, 4 months ago) |
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sweet thread, good luck
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: cloning sclerotia [Re: Aero]
#20896974 - 11/27/14 12:55 PM (9 years, 4 months ago) |
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Quote:
Aero said: This is how i make the syringes from colonized agar jars
Once the agar is 100% colonized i make a few syringes with sterile water
As u can see it on the picture the jars i use have metal lids, i put two holes on them. one is with polyfil the other is with RTV silicone.
the following is happening inside the glovebox:
I squirt some water in the jar then with circular motions the water washes the agar off the wall.
Next step I get a clean brand new ziplock baggie. Open the jar open the baggie, slip the agar into the baggie and close it.
U got some water u got the fully colonized agar wedge ready to rock. Massage the agar then press it till it falls apart into tiny pieces. some agar will be more difficult to break depends on the mix really.
once its done, get some IPA and spray the side of the bag. wipe it clean then poke a whole with the needle, squirt some more water in then start sucking the water back up. when it stops squirt some in the suck it back again, ull get bigger clumps blocking the needle but with a bit of patient ull get a syringe full of colonized agar pieces floating in the water
ive been using this method for quite some time and never failed me
hope it helps for some to extend cultures to jars and so on comes very handy when u are running out of something or just on the last jar, put some colonized grain on agar wait till its colonized then make a few syringes out of it
enjoy
ah the old bag method! gotta love a ziplock.
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Yea man. Never fails me!
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ToxicDuck
Burt Macklin - F.B.I.
Registered: 08/25/14
Posts: 1,021
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Re: cloning sclerotia [Re: Aero]
#20897629 - 11/27/14 04:15 PM (9 years, 3 months ago) |
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Looking forward to more updates.
I also must add that I'm super jealous that you can just order stones .
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: ToxicDuck]
#20897693 - 11/27/14 04:42 PM (9 years, 3 months ago) |
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gonna soak some rye and shoot the jars with the Atl7 clones
-------------------- SPREAD THE SPORES
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: Aero]
#20905053 - 11/29/14 04:04 PM (9 years, 3 months ago) |
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growth in all jars made from the second batch of stones
-------------------- SPREAD THE SPORES
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eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: cloning sclerotia [Re: Aero]
#20905065 - 11/29/14 04:08 PM (9 years, 3 months ago) |
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ToxicDuck
Burt Macklin - F.B.I.
Registered: 08/25/14
Posts: 1,021
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Re: cloning sclerotia [Re: Aero]
#20907198 - 11/30/14 06:05 AM (9 years, 3 months ago) |
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Quote:
Aero said: growth in all jars made from the second batch of stones
Nice !
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: ToxicDuck]
#20995308 - 12/18/14 05:29 PM (9 years, 3 months ago) |
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Clone wars
Tampanensis Mexicana Mexicana pajarito Galindoi Atl#7
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Edited by Aero (12/18/14 05:46 PM)
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Re: cloning sclerotia [Re: Aero]
#20995312 - 12/18/14 05:30 PM (9 years, 3 months ago) |
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,
Got any extra stone producers you want to send to a fan?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: taGyo]
#20995387 - 12/18/14 05:45 PM (9 years, 3 months ago) |
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Next time try pm. This isn't the marketplace really -_-
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Re: cloning sclerotia [Re: Aero]
#20995399 - 12/18/14 05:48 PM (9 years, 3 months ago) |
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I didn't even expect a yes ,
Wasn't really a marketplace question anyway
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: taGyo]
#20995469 - 12/18/14 06:05 PM (9 years, 3 months ago) |
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If u u might get lucky
-------------------- SPREAD THE SPORES
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: Aero]
#21035837 - 12/28/14 02:51 PM (9 years, 3 months ago) |
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9days after inoculation
The ATL7 the Pajarito already shows sclerotia veins lookin good so far, very aggressive colonizers, the mexicana has the lightest mycelium out of the four
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Aero
Orea
Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 4 months
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Re: cloning sclerotia [Re: Aero]
#21035957 - 12/28/14 03:23 PM (9 years, 3 months ago) |
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ATL7 closeup
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 5 months
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Re: cloning sclerotia [Re: Aero]
#21035969 - 12/28/14 03:26 PM (9 years, 3 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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