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 beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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 noob question 
#20720491 - 10/18/14 02:16 PM (9 years, 5 months ago) |
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Ok I'm trying to get a better understanding of what I'm doing!! It all started with 2 12cc syringes of a Classic PF strain. I read somewhere the genetics that were crossed to create this strain but can't find it now? I started with 4 jars, 1 half colonized I cut it and fruited and the 2nd one fully colonized I let it consolidate(1week) with the D&R then into SGFC. It's just starting to pin now so I'm excited for that :-)) **BUT Before I rolled that cake..... I made up 2 of my own BRF mix jars.... I sterilized a small area cut a small piece of the cake w sterile knife and chopped up finely in sterile water, sucked it up in a syringe and nocked the 2 jars.... 3 days later growth!!!! My question is with a multistory syringe am I getting random genetics and how long can I keep cutting and making my own jars off this one strain?? How does it work??!!??
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beave928
Young Grasshoppa
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 Re: noob question [Re: beave928]
#20720500 - 10/18/14 02:17 PM (9 years, 5 months ago) |
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PurePleasure
Untrusted bacteria cultivator.
Registered: 08/29/14
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Re: noob question [Re: beave928]
#20720602 - 10/18/14 02:45 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
My question is with a multistory syringe am I getting random genetics
A multispore syringe is filled with random genetics.
I'm kind of confused...
You sterilized a part of your brf cake...?
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
 
Edited by PurePleasure (10/18/14 02:46 PM)
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 wowimflabbergasted
supercalifragilistic
Registered: 07/16/12
Posts: 18,919
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Multispore is random genetics and agar is what you are looking for to isolate and keep genetics going. 
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beave928
Young Grasshoppa
Registered: 09/07/14
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Ok but if I ordered a specific strain would it be a multispore? It was called PF Classic... And no I didn't sterilize the cake in any way I just cut a small piece of the fully colonized cake and cut it up into tiny tiny pieces and sucked it up into sterile water and injected into jars
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PurePleasure
Untrusted bacteria cultivator.
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Re: noob question [Re: beave928]
#20720749 - 10/18/14 03:27 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Ok but if I ordered a specific strain would it be a multispore? It was called PF Classic... And no I didn't sterilize the cake in any way I just cut a small piece of the fully colonized cake and cut it up into tiny tiny pieces and sucked it up into sterile water and injected into jars
yep.
ms syringe is pretty much the only legal way to send spores. that or a print.
and your "pf classic" strain is a marketing gimmick.
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
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wowimflabbergasted
supercalifragilistic
Registered: 07/16/12
Posts: 18,919
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Quote:
PurePleasure said:
Quote:
beave928 said:
Ok but if I ordered a specific strain would it be a multispore? It was called PF Classic... And no I didn't sterilize the cake in any way I just cut a small piece of the fully colonized cake and cut it up into tiny tiny pieces and sucked it up into sterile water and injected into jars
yep.
ms syringe is pretty much the only legal way to send spores. that or a print.
and your "pf classic" strain is a marketing gimmick.
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
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Lol how funny someone used his screen name to make money lol!!! Silly me to believe he made a "holy grail" strain and I had it!!?? Is there anyway to tell what strain it mighta been? I have pics of some fruits
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Re: noob question [Re: beave928]
#20720932 - 10/18/14 04:12 PM (9 years, 5 months ago) |
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Oh and I'd love to start working with agar but I need to invest in a pressure cooker first! That an I want to keep experimenting and working on sterile technique. I would love to jump right into A2G and G2G spawned to bulk. Can't do any of it without a PC
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Munchauzen
Registered: 06/22/11
Posts: 14,344
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Re: noob question [Re: beave928]
#20721011 - 10/18/14 04:41 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Oh and I'd love to start working with agar but I need to invest in a pressure cooker first! That an I want to keep experimenting and working on sterile technique. I would love to jump right into A2G and G2G spawned to bulk. Can't do any of it without a PC
check out thrift stores like Goodwill. you can used pressure cookers for super cheap
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beave928
Young Grasshoppa
Registered: 09/07/14
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Last seen: 9 years, 5 months
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Small town they don't come up often.. I can pick up a brand new one at a giant country feed and supply store. They got everything I need for this lol! Verm, perlite, tubs, fresh Hpoo, rye berries, PC the nice all American ones, the short 1/2 pint jars, it's just having the extra cash to purchase everything I need to start going to bulk... I wana try a mono tub with ground up cakes and Hpoo but I don't quite understand if I need to fan and mist a mono tub or just leave it closed an let it do it's thing I live in AZ we don't have much humidity
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TheMustardTiger
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Re: noob question [Re: beave928]
#20721114 - 10/18/14 05:07 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Small town they don't come up often.. I can pick up a brand new one at a giant country feed and supply store. They got everything I need for this lol! Verm, perlite, tubs, fresh Hpoo, rye berries, PC the nice all American ones, the short 1/2 pint jars, it's just having the extra cash to purchase everything I need to start going to bulk... I wana try a mono tub with ground up cakes and Hpoo but I don't quite understand if I need to fan and mist a mono tub or just leave it closed an let it do it's thing I live in AZ we don't have much humidity
Start out with a coir based bulk substrate until you get the hang of it. And no, a properly dialed in mono tub requires no misting at all unless you're replenishing moisture after a flush.
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spacechildo
proletarians rise up
Registered: 01/24/13
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Re: noob question [Re: beave928]
#20721139 - 10/18/14 05:13 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Ok but if I ordered a specific strain would it be a multispore? It was called PF Classic... And no I didn't sterilize the cake in any way I just cut a small piece of the fully colonized cake and cut it up into tiny tiny pieces and sucked it up into sterile water and injected into jars
looks like you got a bit of bacteria in there as well. your method is far from fool proof,
just grow out the shrooms and take a new print!
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Re: noob question [Re: beave928]
#20721197 - 10/18/14 05:32 PM (9 years, 5 months ago) |
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And!!!! #its growing wayyy faster than spores :-)
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PurePleasure
Untrusted bacteria cultivator.
Registered: 08/29/14
Posts: 5,893
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Re: noob question [Re: beave928]
#20721203 - 10/18/14 05:33 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Lol how funny someone used his screen name to make money lol!!! Silly me to believe he made a "holy grail" strain and I had it!!?? Is there anyway to tell what strain it mighta been? I have pics of some fruits
yes.
it looks like a cubensis strain
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
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spacechildo
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Re: noob question [Re: beave928]
#20721208 - 10/18/14 05:34 PM (9 years, 5 months ago) |
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yep, but its hard to get the process done cleanly. like the bacteria in your jars show.
Quote:
beave928 said:
try this, Muda's LI tek
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Quote:
spacechildo said:
Quote:
beave928 said:
Ok but if I ordered a specific strain would it be a multispore? It was called PF Classic... And no I didn't sterilize the cake in any way I just cut a small piece of the fully colonized cake and cut it up into tiny tiny pieces and sucked it up into sterile water and injected into jars
looks like you got a bit of bacteria in there as well. your method is far from fool proof,
just grow out the shrooms and take a new print!
I know it's far from fool proof I guess I could pull a print off one if the caps just coming in now? Why do you say contam??! That's been said before about my jar and it wasn't correct? The source who said it was wayyyyy more qualified than my young grasshopper self but I truly believe I had terrible he Which caused the jar to stall
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Re: noob question [Re: beave928]
#20721217 - 10/18/14 05:36 PM (9 years, 5 months ago) |
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But heck if I can get 2 jars to fully colonize I'd say I've got a pretty dang good sterile technique ehh??
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Re: noob question [Re: beave928]
#20721223 - 10/18/14 05:37 PM (9 years, 5 months ago) |
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The thing about spores is they take sooooo long to germinate :-(.
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spacechildo
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Re: noob question [Re: beave928]
#20721235 - 10/18/14 05:40 PM (9 years, 5 months ago) |
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nah just start the new batch a few days earlier. 3-5 days is normal with fresh spores.
what does "terrible he" means? I didnt say contam I said bacteria.
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Sorry terrible GE (gas exchange) and it cause my half colonized jar to stall.... Ok so why do u assume i have a bacterial contam??
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Re: noob question [Re: beave928]
#20721296 - 10/18/14 05:54 PM (9 years, 5 months ago) |
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If I get this shit to work for me more than once can someone give me some shroomery cred and rate a brotha :-).
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spacechildo
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Re: noob question [Re: beave928]
#20721303 - 10/18/14 05:56 PM (9 years, 5 months ago) |
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how can you have bad GE in a jar with 4 holes?
I dont assume, I can tell thats bacteria because of the way it looks.
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PurePleasure
Untrusted bacteria cultivator.
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Quote:
spacechildo said:
yep, but its hard to get the process done cleanly. like the bacteria in your jars show.
Quote:
beave928 said:
try this, Muda's LI tek
Wait wait wait..
hold the phone.
are you using coffee filters as a filter media?
do you still have a verm barrier?
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
Edited by PurePleasure (10/18/14 06:15 PM)
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beave928
Young Grasshoppa
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Last seen: 9 years, 5 months
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I still have a dry verm layer that's just for the steaming processes and foil over it... The jar didn't have 4 nail holes it had a self healing nock point and a lil filter that got clogged with verm and didn't allow any gas exchange
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PurePleasure
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Re: noob question [Re: beave928]
#20721426 - 10/18/14 06:30 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
I still have a dry verm layer that's just for the steaming processes and foil over it... The jar didn't have 4 nail holes it had a self healing nock point and a lil filter that got clogged with verm and didn't allow any gas exchange
the brf method with 4 holes is the simplest.
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
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beave928
Young Grasshoppa
Registered: 09/07/14
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Last seen: 9 years, 5 months
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Quote:
spacechildo said:
I dont assume, I can tell thats bacteria because of the way it looks.
I'm not calling you a liar but I don't think so an I really hope not!! Read my very first post PussyFart made the comment that I had bacterial growth but I don't feel that it was. It had that perfect mushy smell. I've read you will know the smell when u have a bacterial contam? Myc won't colonize over bacteria so why did my cake colonize %100 if i had a contam? All my jars look exactly the same and fruiting them with no problems?
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Quote:
PurePleasure said:
Quote:
beave928 said:
the brf method with 4 holes is the simplest.
I realize that now... I intend on buying a case of the short wide 1/2 pints and poking 4 nail holes with a dry verm layer and I should ever have a prob w GE and contams can't seem to get past dry verm :-)
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PurePleasure
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Re: noob question [Re: beave928]
#20721441 - 10/18/14 06:34 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Quote:
spacechildo said:
I dont assume, I can tell thats bacteria because of the way it looks.
I'm not calling you a liar but I don't think so an I really hope not!! Read my very first post PussyFart made the comment that I had bacterial growth but I don't feel that it was. It had that perfect mushy smell. I've read you will know the smell when u have a bacterial contam? so why did my cake colonize %100 if i had a contam? All my jars look exactly the same and fruiting them with no problems?
Yep your right, pussyfart must be wrong if you dont think so. You know.. since your jars didn't smell like bacteria
Quote:
Myc won't colonize over bacteria
This is FALSE
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
Edited by PurePleasure (10/18/14 06:34 PM)
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beave928
Young Grasshoppa
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Re: noob question [Re: beave928]
#20721451 - 10/18/14 06:36 PM (9 years, 5 months ago) |
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If there was say a 3/8 diameter hole on top where I removed the rubber self healing thing would it be too big a hole to leave open with a dry verm layer? I've been putting a piece of micro spore tape over the big hole I was worried a contam can get in if it's a big hole?
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beave928
Young Grasshoppa
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Re: noob question [Re: beave928]
#20721460 - 10/18/14 06:38 PM (9 years, 5 months ago) |
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Don't put words in my mouth I never said he was wrong? Nobody ever explained how that works?! Will they grow together and still fruit? Can a jar still look and be %100 colonized of it has bacteria??
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beave928
Young Grasshoppa
Registered: 09/07/14
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Re: noob question [Re: beave928]
#20721471 - 10/18/14 06:40 PM (9 years, 5 months ago) |
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I said I don't feel that statement was correct... That's my noob ass opinion everyone has one and it's not any easier when nobody explained how a bacterial contam works... I asked if anyone could further explain but noooooo... Just that I had bacterial growth... Sounds like that's all I grow lol I'm a bacterial farmer lol
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spacechildo
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Re: noob question [Re: beave928]
#20721543 - 10/18/14 06:52 PM (9 years, 5 months ago) |
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the white stuff is bacteria.
just use 4 puncture holes, cover with micropore tape and inoculate through the tape.
www.mushroomvideos.com
edit; if you wanna see what bacteria looks like close up checkout purepleasure's threads
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PurePleasure
Untrusted bacteria cultivator.
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Quote:
spacechildo said:
the white stuff is bacteria.
just use 4 puncture holes, cover with micropore tape and inoculate through the tape.
www.mushroomvideos.com
edit; if you wanna see what bacteria looks like close up checkout purepleasure's threads
Sorry, I couldn't help but laugh at this either...
My sig is proof of bacterial sub potential 
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
Edited by PurePleasure (10/18/14 11:27 PM)
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PurePleasure
Untrusted bacteria cultivator.
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Re: noob question [Re: beave928]
#20722642 - 10/18/14 11:41 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Can a jar still look and be %100 colonized of it has bacteria??
yep, depending on the extent of the bacteria present and what kind.
Ive seen bacteria so strong the jars cant even germinate spores and grow mycelium.
I've also seen cubensis mycelium woop some bacterial ass plenty of times leaving only a path of smoking metabolites behind.
unfortunately, It is a constant battle for the most part 
bacteria usually never truly leaves though, just waits for the mycelium to become weak and to exhausted to fight.
again, the extent of bacteria plays the role,
I've had bacterial subs go as far as 5 and 6 flushes.
I have had bacterial subs put out 1 canopy, and the rest were about 2 inches tall and just exhausted lol.
OP,
Don't be discouraged
Most of your jars look like they are going to be healthy enough to put out some beautiful fruits for you 
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Making more and more sense!!!! Thank you so much pure pleasure!!! I needed an explanation like that the first time :-))) wow wow wow I love this hobby :-).
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beave928
Young Grasshoppa
Registered: 09/07/14
Posts: 71
Last seen: 9 years, 5 months
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Re: noob question [Re: beave928]
#20723752 - 10/19/14 10:25 AM (9 years, 5 months ago) |
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The metabolites that get left behind? Is that the "myc piss" or yellow stuff ppl speak of???
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PurePleasure
Untrusted bacteria cultivator.
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Re: noob question [Re: beave928]
#20724016 - 10/19/14 11:34 AM (9 years, 5 months ago) |
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Quote:
beave928 said:
The metabolites that get left behind? Is that the "myc piss" or yellow stuff ppl speak of???
Do me a favor and dont ever say myc piss again  .
That is metabolites.
Metabolites are usually present when the myc is under stress.
Like coming in contact with a contam or bacteria.
Most of your antibiotics are made from these metabolites that mushrooms produce.
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
Edited by PurePleasure (10/19/14 11:40 AM)
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beave928
Young Grasshoppa
Registered: 09/07/14
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Last seen: 9 years, 5 months
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Hahaha you got it!! Heard it around the forum before lol!! Anyway yup I had it In my fully colonized jar so there had to be a battle at jar hill going on in there!!
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PurePleasure
Untrusted bacteria cultivator.
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Re: noob question [Re: beave928]
#20725208 - 10/19/14 04:28 PM (9 years, 5 months ago) |
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Quote:
beave928 said:
Hahaha you got it!! Heard it around the forum before lol!! Anyway yup I had it In my fully colonized jar so there had to be a battle at jar hill going on in there!!
As long as your cakes colonize 100%, then go for it
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If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)
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