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 firstgrowneedhelp
Stranger
Registered: 07/13/14
Posts: 15
Last seen: 9 years, 6 months
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 Is this mycelium? 
#20320954 - 07/24/14 11:18 AM (9 years, 8 months ago) |
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http://pinkfloyyyd.imgur.com/all/
Is this mycelium or contam or something else? It doesn't look like either, at least from what I've seen in pictures. Perhaps a problem with growing conditions?
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 mushmagic
supporting radical habits
 
Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Can't really see anything from those pics..
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mehdichi
Registered: 06/12/14
Posts: 152
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yup, definitely need better pics, all i see is verm and brf
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firstgrowneedhelp
Stranger
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Last seen: 9 years, 6 months
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Re: Is this mycelium? [Re: mushmagic]
#20321026 - 07/24/14 11:42 AM (9 years, 8 months ago) |
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I'll try to take some better pictures when my phone charges. Just without the pictures for now though, the white specks are pretty evenly distributed throughout the substrate, although one jar has a large mass of them pressed up against the glass. I'm thinking maybe when I inocculated I didn't tilt the jars right so the spores never ran down the side of the glass and instead went straight to the middle. If the mycelium grew from the middle out instead of the sides in, I guess it would make sense that it would be colonizing the whole jar at once. But it still looks different, instead of looking like it's woven out of many strands it sort of looks like little pieces of cooked white rice maybe?
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mehdichi
Registered: 06/12/14
Posts: 152
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im guessing it looks something like this? (one of my jars)
if those little white specs everywhere is what you mean, then there is nothing to worry about. its not contam or myc, its just the brf/verm, i think it gets like that when it gets wet. your fine man, just leave them be and they will start colonizing in no time!
edit: new picture
Edited by mehdichi (07/24/14 11:50 AM)
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firstgrowneedhelp
Stranger
Registered: 07/13/14
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Last seen: 9 years, 6 months
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Re: Is this mycelium? [Re: mehdichi]
#20321172 - 07/24/14 12:24 PM (9 years, 8 months ago) |
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http://pinkfloyyyd.imgur.com/all
Better pictures. My first thought was that it was just BRf, but does steaming it really make all that flour that was dispersed throughout the jar clump up like that? I mean some of the specks are pretty large, others I could believe are BRF.
If it is just BRF, I'm sure the lack of mycelium growth can be attributed to the fact that after sterilizing, I stupidly left the tin foil on the jars during several days of incubation, which would affect air flow. Can the mycelium bounce back after being suffocated like that?
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mehdichi
Registered: 06/12/14
Posts: 152
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dont worry about it man, next time just make sure there is some holes to allow gas exchange. and from the looks of it, that jar is pretty big. for brf/verm you definately want half pint-jars, wide mouth preferably. those big jars usually stall out
**** i definately dont see any mycelium though. so just let them sit and hope you see some in the next few days/weeks !
Edited by mehdichi (07/24/14 12:30 PM)
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Pastywhyte 
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Re: Is this mycelium? [Re: mehdichi]
#20321204 - 07/24/14 12:31 PM (9 years, 8 months ago) |
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The foil won't inhibit GE much but it will cause your dry verm layer to get damp which can wick contams.
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firstgrowneedhelp
Stranger
Registered: 07/13/14
Posts: 15
Last seen: 9 years, 6 months
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Re: Is this mycelium? [Re: mehdichi]
#20321226 - 07/24/14 12:37 PM (9 years, 8 months ago) |
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Yeah, I definitely will get some half pints if these big jars don't work. Luckily I saved some of the spore syringe and started a LC, so hopefully I won't need to buy anything else through the mail. Thanks for the help 
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firstgrowneedhelp
Stranger
Registered: 07/13/14
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Last seen: 9 years, 6 months
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Good to hear that gas exchange was still happening, hopefully it doesn't get contaminated because of that though.
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada
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I would not use an LC inoculated with spores 
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firstgrowneedhelp
Stranger
Registered: 07/13/14
Posts: 15
Last seen: 9 years, 6 months
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I know LC's are risky but I'm willing to take the chance of contamination if I might avoid buying another spore syringe.
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada
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You might feel differently if it contams on you. Why not just take a print and make your own syringe? Better yet take a print and use it on agar. Once you start agar you will never need another syringe. Unless you want a new variety. But you can trade for those easy enough so really once you start agar you never need to buy spores ever again 
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firstgrowneedhelp
Stranger
Registered: 07/13/14
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Last seen: 9 years, 6 months
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Right, I would definitely prefer to do that. I'll only use the LC if all of these jars fail, if they fruit then I'll get some prints.
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wowimflabbergasted
supercalifragilistic
Registered: 07/16/12
Posts: 18,919
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Quote:
Pastywhyte said:
You might feel differently if it contams on you. Why not just take a print and make your own syringe? Better yet take a print and use it on agar. Once you start agar you will never need another syringe.
 Why make an LC and not some agar?
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Have fun combating doubts every step of the way with that LC.
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Donsanpedro
Stranger
Registered: 07/05/14
Posts: 268
Last seen: 9 years, 8 months
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Re: Is this mycelium? [Re: Inocuole]
#20321805 - 07/24/14 03:16 PM (9 years, 8 months ago) |
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I don't see mycelium. Sorry. But only time will tell. GL
DSP
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firstgrowneedhelp
Stranger
Registered: 07/13/14
Posts: 15
Last seen: 9 years, 6 months
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I've never heard of agar before. I read a few sources but can someone give me a quick rundown of what it is, why it's better than LC, and what I need in order to do it?
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Sanguin3
Optimist
Registered: 10/19/13
Posts: 2,273
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Re: Is this mycelium? *DELETED* [Re: Pastywhyte]
#20324210 - 07/24/14 10:34 PM (9 years, 8 months ago) |
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Post deleted by Sanguin3
Reason for deletion: .
Edited by Sanguin3 (07/24/14 10:35 PM)
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firstgrowneedhelp
Stranger
Registered: 07/13/14
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Last seen: 9 years, 6 months
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Re: Is this mycelium? [Re: Sanguin3]
#20324904 - 07/25/14 01:24 AM (9 years, 8 months ago) |
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I don't have a way to get a PC 
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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can't get 80 bux? shitty deal how were you going to do a lc then?
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   It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheEaglesGift
The Nagual
Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Is this mycelium? [Re: cronicr]
#20325098 - 07/25/14 03:27 AM (9 years, 8 months ago) |
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You'd be better off dropping the 17 bucks on a new syringe over wasting your time/money/effort on an LC. LC's can work, but they're very hit or miss. I don't muck with them anymore at all. I got sick of being a bacteria farmer.
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firstgrowneedhelp
Stranger
Registered: 07/13/14
Posts: 15
Last seen: 9 years, 6 months
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Re: Is this mycelium? [Re: cronicr]
#20327751 - 07/25/14 04:49 PM (9 years, 8 months ago) |
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I found a tek that used steam sterilization with a pot.
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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don't bother dude
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It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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firstgrowneedhelp
Stranger
Registered: 07/13/14
Posts: 15
Last seen: 9 years, 6 months
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For my LC I spent 0$ (used random jar and the last bit of my spore syringe) and it took about an hour, most of which was spent doing something else while the jar sterilized. So even if it gets contaminated, the cheapness and easiness of LC's (especially the fact that I can steam sterilize without a PC) makes their risk worth it to me.
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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good luck
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It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Is this mycelium? [Re: cronicr]
#20328039 - 07/25/14 05:53 PM (9 years, 8 months ago) |
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Making the LC isn't what's risky. It's giving it weeks to colonize, then injecting it in a dozen jars and every one of them contaminating that's risky.
Sure you could try again, but that's another couple weeks and more spores every time it fails.
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firstgrowneedhelp
Stranger
Registered: 07/13/14
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Last seen: 9 years, 6 months
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Re: Is this mycelium? [Re: Inocuole]
#20341662 - 07/28/14 07:14 AM (9 years, 8 months ago) |
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Maybe I'm missing something that more experience would tell me, but what's wrong with just inocculating 1 jar as a trial for each new LC?
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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That's what people typically do, but it will still take weeks to know.
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada
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Nothing, a test jar is a must with LC. But it takes time to grow and once it is you still need to inoculate your jars and in the time where you were waiting for your test jar to grow an endospore could have germinated or a filter failed and a mold spore got in. This is why LC is risky. It can be done but great care and proper sterile procedure needs to be adhered to if you don't want everything to shit the bed.
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Fivel
Crazy Smurf
Registered: 07/09/14
Posts: 521
Loc: Midwest
Last seen: 2 years, 28 days
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Cause that won't tell you 100% whether the LC is clean or not.There is so many ways to contam a LC. You could contaminate it while you are making the test jar and the test jar ends up fine but when you nocc up 20 jars with it thinking its fine and they all go bad. Problem is you can't spot contaminates in liquid. However if you make agar petri's, you can squirt a drop of your lc in a petri, let it grow, and if its contam you can 9 times out of 10 transfer a healthy piece of mycelium to a new plate and let that grow clean. Agar is a much more forgiving medium than liquid cultures. Everything you need to know can be found in these threads btw.
Making agar --> http://www.shroomery.org/forums/showflat.php/Number/19208976
Cleaning --> http://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/1/vc/1/nt/4
And of course what it should and shouldn't look like --> http://www.shroomery.org/forums/showflat.php/Number/17231313#17231313
Those three threads saved my operation.
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Edited by Fivel (07/28/14 07:53 AM)
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