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TheFog
Mycophobe
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
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Proper SAB construction.
#19891390 - 04/24/14 03:34 AM (9 years, 11 months ago) |
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I've learned so much from this site and, like always, I'd like to learn more.
Please post your guides to proper SAB (Still Air Box) construction. I looked through the trusted cultivators and AMU guides but have not been able to find anything.
I recently stepped up my game and bought a PC. I have a lab coat, sleeves, gloves, alcohol lamp, etc. Seems to me most of you are using SAB without fixed gloves and rubber mounts. I'm not a science major so I don't know the proper terms. I just know that the pics I've seen are definitely not lab safe.
Any help is appreciated.
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Proper SAB construction. [Re: TheFog]
#19891414 - 04/24/14 03:45 AM (9 years, 11 months ago) |
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biggest clear tote u can find (100qts is good) one coffee can, one gas stove, burn two holes shoulder width apart, and ur done. use tyvek sleeves when doing work in an sab
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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I.P.Cleanly
Stranger
Registered: 02/16/14
Posts: 25
Last seen: 9 years, 11 months
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I use a hole saw... drill slowly, some people drill in reverse... I've never had to.
Really one of the easier projects in this hobby.
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TheApprentice
back at it
Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 3 months
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Re: Proper SAB construction. [Re: I.P.Cleanly]
#19891670 - 04/24/14 05:40 AM (9 years, 11 months ago) |
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Take a tote, measure some arm holes.
Flame tip an old knife to cut thru plastic.
Easy
-------------------- RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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toxetel
׀ǝʇǝxoʇ ●
Registered: 08/13/12
Posts: 708
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Re: Proper SAB construction. [Re: TheFog]
#19891676 - 04/24/14 05:43 AM (9 years, 11 months ago) |
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Quote:
TheFog said: I'm not a science major so I don't know the proper terms. I just know that the pics I've seen are definitely not lab safe.
What do you believe is "lab safe?"
Attaching gloves to a plastic tote is worse than just having holes in the side. The lid does not a have a perfect seal, and the air inside is not sterile. So if you attached gloves your moving hands would cause the whole system to act as a pump, pulling air in through the cracks and swirling contaminants around.
Also, how are you gonna flame-sterilize your needles, scalpels, and loops if your gloves are stuck to the box? You can't burn the alcohol lamp inside the box.
Like these guys have said, all you need to do is take a big plastic box and cut two holes in it, about shoulder-width apart. As these guys have said, you can use a hole saw, melt the holes by heating up a coffee/soup/whatever can on the stove or with a propane torch, heat up a knife... even use a drill to perforate the boudnaries of a circle and then cut/punch through the little pieces.
The PF Tek chapter of RR's videos show what it should look like (though I think he calls it a 'glovebox'). FrankHorrigan's beginner's tek also describes how to make one. If you're having a hard time finding a clear box, you can cut a window into the top of an opaque one, covering it with some kind of clear plastic.
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TheApprentice
back at it
Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 3 months
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Re: Proper SAB construction. [Re: toxetel]
#19891869 - 04/24/14 07:04 AM (9 years, 11 months ago) |
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my SAB rocks.
i just wear a lab coat or long sleeved shirt
no contams so for.
in fact, since i've started using the SAB i havent even had contams when i take clones.
-------------------- RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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TheFog
Mycophobe
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
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Heating a coffee can is a great idea, never thought of it. I didn't want to shell out $40 for a 4" hole cutter.
I see some people prefer butane torches over alcohol lamps. Could I use the torch as needed inside the SAB? I've just been using regular Bic lighters so far.
Thanks everyone.
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Bulbasaur
Droppin loads
Registered: 04/18/14
Posts: 329
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Re: Proper SAB construction. *DELETED* [Re: TheFog]
#19896786 - 04/25/14 03:52 AM (9 years, 10 months ago) |
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Post deleted by BulbasaurReason for deletion: \
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toxetel
׀ǝʇǝxoʇ ●
Registered: 08/13/12
Posts: 708
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Re: Proper SAB construction. [Re: TheFog]
#19896990 - 04/25/14 06:20 AM (9 years, 10 months ago) |
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Quote:
TheFog said: I see some people prefer butane torches over alcohol lamps. Could I use the torch as needed inside the SAB?
No, and you shouldn't be using a lighter inside your still-air box either! For one thing, a flame causes significant air movement, the opposite of what a still-air box is trying to achieve. And for another, if you've used alcohol to sanitize anything inside the box, the fumes will hang around in there and could ignite. More than one Shroomery newbie has had an SAB explosion because of this.
Just keep the flame outside the box. Take your arm out of the hole with the instrument in it, flame sterilize it, and get it back in before the instrument cools off.
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TheApprentice
back at it
Registered: 09/25/11
Posts: 4,727
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Re: Proper SAB construction. [Re: toxetel]
#19896993 - 04/25/14 06:21 AM (9 years, 10 months ago) |
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Quote:
toxetel said:
Quote:
TheFog said: I see some people prefer butane torches over alcohol lamps. Could I use the torch as needed inside the SAB?
No, and you shouldn't be using a lighter inside your still-air box either! For one thing, a flame causes significant air movement, the opposite of what a still-air box is trying to achieve. And for another, if you've used alcohol to sanitize anything inside the box, the fumes will hang around in there and could ignite. More than one Shroomery newbie has had an SAB explosion because of this.
Just keep the flame outside the box. Take your arm out of the hole with the instrument in it, flame sterilize it, and get it back in before the instrument cools off.
-------------------- RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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TheFog
Mycophobe
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
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Re: Proper SAB construction. [Re: Bulbasaur]
#19930789 - 05/02/14 05:09 AM (9 years, 10 months ago) |
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Quote:
Bulbasaur said: I like how TranscendingLife does his thangs
http://www.shroomery.org/forums/showflat.php/Number/14722730#14722730
Followed that guide exactly.
The sleeves were a nightmare with sharp needles. I will save them for G2G.
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 9,866
Last seen: 20 days, 19 hours
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Re: Proper SAB construction. [Re: TheFog]
#19930804 - 05/02/14 05:20 AM (9 years, 10 months ago) |
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You could still keep one. Only takes one hand to flame a needle.
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TheFog
Mycophobe
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
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Re: Proper SAB construction. [Re: Kizzle]
#19930817 - 05/02/14 05:40 AM (9 years, 10 months ago) |
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Quote:
Kizzle said: You could still keep one. Only takes one hand to flame a needle.
Yeah, it makes sense to do that.
I never let my left hand out of the box, poked plenty of holes in the right sleeve before I ripped it out. Tried bringing in syringes backwards but they hit the sleeve so… contam-ville. Also, there is no reason to do this with at hole saw… it was $40 and I could have done just as good of a job with a hot knife or a small sauce pan.
My SAB is only going to be used for G2G transfers from now on. Sleeves might be a good idea for that. But for inoculation from liquid syringes, no thanks.
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Quexl
Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 9 years, 1 month
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Re: Proper SAB construction. [Re: TheFog]
#19930880 - 05/02/14 06:25 AM (9 years, 10 months ago) |
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I know you've already got things constructed but ... You can just flip the box on it's side cover 2/3rds of the opening in seran wrap too, so there's a slot at the bottom for your hands. It works fine. You don't need to melt\cut perfectly circular holes in it at all, and you don't absolutely need sleeves either. imo the more things going on in that space the more likely you'll just frustrate your attempt at being sterile trying to fumble things around.
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TheFog
Mycophobe
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
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Re: Proper SAB construction. [Re: Quexl]
#19930897 - 05/02/14 06:38 AM (9 years, 10 months ago) |
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I felt pretty good about building such a contraption. Learned a lot and I definitely think I have more sterile procedure now.
The box and lid are clear )). Sleeves seemed to limit airflow, and that's what we're after, yeah? One pro trick I used: wash down the SAB with dish soap and don't clean it up or let it dry. Bacteria / fungal spores will stick to the wet areas from increased surface tension. Same reason you put a drop of soap into fruit fly traps.
The more you know!!!
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Quexl
Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 9 years, 1 month
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Re: Proper SAB construction. [Re: TheFog]
#19930936 - 05/02/14 06:59 AM (9 years, 10 months ago) |
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Soap actually decreases the surface tension of water. If you mist the inside of the the SAB with straight water before you use it it'll physically drop all the aerosols out of the air too.
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