Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineTheFog
Mycophobe
 User Gallery
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
Proper SAB construction.
    #19891390 - 04/24/14 03:34 AM (9 years, 11 months ago)

I've learned so much from this site and, like always, I'd like to learn more.

Please post your guides to proper SAB (Still Air Box) construction. I looked through the trusted cultivators and AMU guides but have not been able to find anything.

I recently stepped up my game and bought a PC. I have a lab coat, sleeves, gloves, alcohol lamp, etc. Seems to me most of you are using SAB without fixed gloves and rubber mounts. I'm not a science major so I don't know the proper terms. I just know that the pics I've seen are definitely not lab safe.

Any help is appreciated.

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Proper SAB construction. [Re: TheFog]
    #19891414 - 04/24/14 03:45 AM (9 years, 11 months ago)

biggest clear tote u can find (100qts is good) one coffee can, one gas stove, burn two holes shoulder width apart, and ur done.  use tyvek sleeves when doing work in an sab


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
OfflineI.P.Cleanly
Stranger
Registered: 02/16/14
Posts: 25
Last seen: 9 years, 11 months
Re: Proper SAB construction. [Re: blindingleaf]
    #19891460 - 04/24/14 04:03 AM (9 years, 11 months ago)

I use a hole saw... drill slowly, some people drill in reverse... I've never had to.

Really one of the easier projects in this hobby.

Extras: Filter Print Post Top
OfflineTheApprentice
back at it
Male User Gallery


Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 3 months
Re: Proper SAB construction. [Re: I.P.Cleanly]
    #19891670 - 04/24/14 05:40 AM (9 years, 11 months ago)

Take a tote, measure some arm holes.

Flame tip an old knife to cut thru plastic.

Easy


--------------------


RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek Easy COIR Trays! *
Pink Oysters on Newspaper TEK

"Yeah?  Well, DRACULA called... and he said he's coming over tonight, and I said OK!" :mastershake:

Extras: Filter Print Post Top
Invisibletoxetel
׀ǝʇǝxoʇ ●


Registered: 08/13/12
Posts: 708
Re: Proper SAB construction. [Re: TheFog]
    #19891676 - 04/24/14 05:43 AM (9 years, 11 months ago)

Quote:

TheFog said:
I'm not a science major so I don't know the proper terms. I just know that the pics I've seen are definitely not lab safe.




What do you believe is "lab safe?"

Attaching gloves to a plastic tote is worse than just having holes in the side. The lid does not a have a perfect seal, and the air inside is not sterile. So if you attached gloves your moving hands would cause the whole system to act as a pump, pulling air in through the cracks and swirling contaminants around.

Also, how are you gonna flame-sterilize your needles, scalpels, and loops if your gloves are stuck to the box? You can't burn the alcohol lamp inside the box.

Like these guys have said, all you need to do is take a big plastic box and cut two holes in it, about shoulder-width apart. As these guys have said, you can use a hole saw, melt the holes by heating up a coffee/soup/whatever can on the stove or with a propane torch, heat up a knife... even use a drill to perforate the boudnaries of a circle and then cut/punch through the little pieces.

The PF Tek chapter of RR's videos show what it should look like (though I think he calls it a 'glovebox'). FrankHorrigan's beginner's tek also describes how to make one. If you're having a hard time finding a clear box, you can cut a window into the top of an opaque one, covering it with some kind of clear plastic.

Extras: Filter Print Post Top
OfflineTheApprentice
back at it
Male User Gallery


Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 3 months
Re: Proper SAB construction. [Re: toxetel]
    #19891869 - 04/24/14 07:04 AM (9 years, 11 months ago)

my SAB rocks.

i just wear a lab coat or long sleeved shirt

no contams so for.

in fact, since i've started using
the SAB i havent even had contams
when i take clones.



--------------------


RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek Easy COIR Trays! *
Pink Oysters on Newspaper TEK

"Yeah?  Well, DRACULA called... and he said he's coming over tonight, and I said OK!" :mastershake:

Extras: Filter Print Post Top
OfflineTheFog
Mycophobe
 User Gallery
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
Re: Proper SAB construction. [Re: TheApprentice]
    #19896742 - 04/25/14 03:06 AM (9 years, 10 months ago)

Heating a coffee can is a great idea, never thought of it. I didn't want to shell out $40 for a 4" hole cutter.

I see some people prefer butane torches over alcohol lamps. Could I use the torch as needed inside the SAB? I've just been using regular Bic lighters so far.

Thanks everyone.

Extras: Filter Print Post Top
InvisibleBulbasaur
Droppin loads

Registered: 04/18/14
Posts: 329
Re: Proper SAB construction. *DELETED* [Re: TheFog]
    #19896786 - 04/25/14 03:52 AM (9 years, 10 months ago)

Post deleted by Bulbasaur

Reason for deletion: \



--------------------

Extras: Filter Print Post Top
Invisibletoxetel
׀ǝʇǝxoʇ ●


Registered: 08/13/12
Posts: 708
Re: Proper SAB construction. [Re: TheFog]
    #19896990 - 04/25/14 06:20 AM (9 years, 10 months ago)

Quote:

TheFog said:
I see some people prefer butane torches over alcohol lamps. Could I use the torch as needed inside the SAB?




No, and you shouldn't be using a lighter inside your still-air box either! For one thing, a flame causes significant air movement, the opposite of what a still-air box is trying to achieve. And for another, if you've used alcohol to sanitize anything inside the box, the fumes will hang around in there and could ignite. More than one Shroomery newbie has had an SAB explosion because of this.

Just keep the flame outside the box. Take your arm out of the hole with the instrument in it, flame sterilize it, and get it back in before the instrument cools off.

Extras: Filter Print Post Top
OfflineTheApprentice
back at it
Male User Gallery


Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 3 months
Re: Proper SAB construction. [Re: toxetel]
    #19896993 - 04/25/14 06:21 AM (9 years, 10 months ago)

Quote:

toxetel said:
Quote:

TheFog said:
I see some people prefer butane torches over alcohol lamps. Could I use the torch as needed inside the SAB?




No, and you shouldn't be using a lighter inside your still-air box either! For one thing, a flame causes significant air movement, the opposite of what a still-air box is trying to achieve. And for another, if you've used alcohol to sanitize anything inside the box, the fumes will hang around in there and could ignite. More than one Shroomery newbie has had an SAB explosion because of this.

Just keep the flame outside the box. Take your arm out of the hole with the instrument in it, flame sterilize it, and get it back in before the instrument cools off.



:zaphod:


--------------------


RR Videos -Best $9 Ever Spent *
No Pour AGAR Tek Easy COIR Trays! *
Pink Oysters on Newspaper TEK

"Yeah?  Well, DRACULA called... and he said he's coming over tonight, and I said OK!" :mastershake:

Extras: Filter Print Post Top
OfflineTheFog
Mycophobe
 User Gallery
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
Re: Proper SAB construction. [Re: Bulbasaur]
    #19930789 - 05/02/14 05:09 AM (9 years, 10 months ago)

Quote:

Bulbasaur said:
I like how TranscendingLife does his thangs

http://www.shroomery.org/forums/showflat.php/Number/14722730#14722730




Followed that guide exactly.



The sleeves were a nightmare with sharp needles. I will save them for G2G.

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,866
Last seen: 20 days, 19 hours
Trusted Cultivator
Re: Proper SAB construction. [Re: TheFog]
    #19930804 - 05/02/14 05:20 AM (9 years, 10 months ago)

You could still keep one. Only takes one hand to flame a needle.


--------------------

Extras: Filter Print Post Top
OfflineTheFog
Mycophobe
 User Gallery
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
Re: Proper SAB construction. [Re: Kizzle]
    #19930817 - 05/02/14 05:40 AM (9 years, 10 months ago)

Quote:

Kizzle said:
You could still keep one. Only takes one hand to flame a needle.




Yeah, it makes sense to do that.

I never let my left hand out of the box, poked plenty of holes in the right sleeve before I ripped it out. Tried bringing in syringes backwards but they hit the sleeve so… contam-ville. Also, there is no reason to do this with at hole saw… it was $40 and I could have done just as good of a job with a hot knife or a small sauce pan.

My SAB is only going to be used for G2G transfers from now on. Sleeves might be a good idea for that. But for inoculation from liquid syringes, no thanks.

Extras: Filter Print Post Top
OfflineQuexl
Male

Registered: 12/17/13
Posts: 1,443
Loc: Flag
Last seen: 9 years, 1 month
Re: Proper SAB construction. [Re: TheFog]
    #19930880 - 05/02/14 06:25 AM (9 years, 10 months ago)

I know you've already got things constructed but ... You can just flip the box on it's side cover 2/3rds of the opening in seran wrap too, so there's a slot at the bottom for your hands. It works fine. You don't need to melt\cut perfectly circular holes in it at all, and you don't absolutely need sleeves either. imo the more things going on in that space the more likely you'll just frustrate your attempt at being sterile trying to fumble things around.

Extras: Filter Print Post Top
OfflineTheFog
Mycophobe
 User Gallery
Registered: 10/04/13
Posts: 200
Last seen: 9 years, 7 months
Re: Proper SAB construction. [Re: Quexl]
    #19930897 - 05/02/14 06:38 AM (9 years, 10 months ago)

I felt pretty good about building such a contraption. Learned a lot and I definitely think I have more sterile procedure now.

The box and lid are clear :smile:)). Sleeves seemed to limit airflow, and that's what we're after, yeah? One pro trick I used: wash down the SAB with dish soap and don't clean it up or let it dry. Bacteria / fungal spores will stick to the wet areas from increased surface tension. Same reason you put a drop of soap into fruit fly traps.

The more you know!!!

Extras: Filter Print Post Top
OfflineQuexl
Male

Registered: 12/17/13
Posts: 1,443
Loc: Flag
Last seen: 9 years, 1 month
Re: Proper SAB construction. [Re: TheFog]
    #19930936 - 05/02/14 06:59 AM (9 years, 10 months ago)

Soap actually decreases the surface tension of water. :smile: If you mist the inside of the the SAB with straight water before you use it it'll physically drop all the aerosols out of the air too.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Alcohol to kill living mold and spores?
( 1 2 3 4 all )
GnuBobo 22,447 63 08/16/16 11:35 AM
by h0ldthedoor
* Diploid's Home-made Alcohol Lamp
( 1 2 3 all )
DiploidM 15,903 57 11/16/08 08:44 AM
by CultiVader
* Re: Alcohol 80% better than 96%!!
( 1 2 all )
paddoholland 5,232 25 03/15/01 09:40 PM
by Shroomzilla
* Alcohol proof?
( 1 2 all )
Juggles 2,986 20 09/24/02 08:00 PM
by Juggles
* colonized PF cakes and alcohol EverlastingNo 972 1 01/15/03 06:47 PM
by Roadkill
* rubbing alcohol harmful? switchstants 2,938 12 03/20/03 04:39 AM
by Anonymous
* Hydrapod Construction, stop sucking up to Mycotek
( 1 2 all )
DolceRequiem 7,869 35 09/05/02 12:01 PM
by Lana
* Non-alcoholic trip???
( 1 2 all )
Sypriss 3,297 29 02/11/02 11:18 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,333 topic views. 13 members, 78 guests and 108 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.01 seconds on 14 queries.