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 Anand
AutisticShroomer
Registered: 11/05/02
Posts: 362
Loc: 30 miles From Amsterdam
Last seen: 30 days, 2 hours
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 Re: Cactus tissue culture. [Re: Openminded]
#1943811 - 09/23/03 08:30 AM (8 years, 8 months ago) |
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Una: will MushMush sell plant Tissue Culture supply's in the future? would be nice.... don't know where to get the MS and other ingredients
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 theshiftingwalls
Divine state
Registered: 06/17/03
Posts: 4,128
Loc: Residing in thee Universa...
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Re: Cactus tissue culture. [Re: Anand]
#1946529 - 09/23/03 10:44 PM (8 years, 8 months ago) |
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He would sell the ingredients to make it possible to culture, get it?
Its all marketing  .
Una you better ship to Canada ! You better!
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Embryogenesis from callus is possible with many species so i don't see why it's not possible with cacti. Of course you would need a good protocol (which i don't have yet).
The shoots of the SP took 3 weeks to develop.
We will not be selling plant TC supplies since most of the growth regulators that are needed are toxic (carry the skull icon) and these would require special (expensive) transport.
Anand if you are really serious about this is might help you out with some stuff. Or you might contact :info@labassociates.com at http://www.labassociates.com/
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www.911blogger.com
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#1957498 - 09/27/03 02:05 PM (8 years, 8 months ago) |
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I set up some pieces yesterday of this cristate/monstrosus pachanoi.
Here's my flow cabinet. Jars wiith medium and sterilised water on the right. Glass bead heater and more sterilised water on the right:
Surface sterilisation with bleach (toilet cleaner  )
Rinsing with sterile water.
Notice the bleach damage (white tissue)
The damaged tissue is cut off. And the remaining piece is divided.
These pieces are put on the multiplication/initiation medium.
No i cross my fingers and hope some of the areoles develop new buds.
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www.911blogger.com
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Anonymous
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Re: Cactus tissue culture. [Re: Una]
#1958488 - 09/27/03 09:31 PM (8 years, 7 months ago) |
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Very interesting stuff. I was talking to my grandmother the other day about growing mushrooms on agar. Then she starts talking about how my greatgrandfather when he was youger got into orchids. He had a full lab and spent all this money on growing thousands of rare orchids. No hybrids. Just pure strains. Then when Hawai came into the picture the market got flooded with orchids. So since he considered himself an artist he took all his plants and equipment and threw them in a pile and burnet them. I wish I had that stuff. Crazy
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#1990544 - 10/08/03 02:50 PM (8 years, 7 months ago) |
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Here's an update on the San Pedro experiment.
I transferred the pieces to fresh medium and cut away some of the callus. The new shoots are developing steadily:
The Trichocereus bridgesii is now also showing some activity:
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www.911blogger.com
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Anonymous
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Re: Cactus tissue culture. [Re: neuro]
#2027654 - 10/21/03 12:33 AM (8 years, 7 months ago) |
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Callus yields genetic variation. Bad idea for maintianing cultivars. Good idea for discovering new ones though.
Stick with the axillary shoot multiplication for maintaining cultivars, while multiplying them.
Those look nice Una.
Research is very limited in cacti microprop. What is out there is usually not reproducible, important info isconvieniently left out of the methodology.
I have some journal studies on the subject of cacti microprop. if you are interested. Explant selection, callus induction, axillary shoot multiplication, somatic embryogenesis, nutritional studies, some specific species studies. There really wasn't much out there as of 1996.
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,609
Loc: Rigel 7
Last seen: 9 months, 28 days
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Re: Cactus tissue culture. [Re: ]
#2028100 - 10/21/03 07:11 AM (8 years, 7 months ago) |
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>>I have some journal studies on the subject of cacti microprop. if you are interested. Explant selection, callus induction, axillary shoot multiplication, somatic embryogenesis, nutritional studies, some specific species studies. There really wasn't much out there as of 1996.
I'd be interested...
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Anonymous
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Re: Cactus tissue culture. [Re: neuro]
#2029409 - 10/21/03 03:25 PM (8 years, 7 months ago) |
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PM me an addy. Which studies are you interested in?
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#2044680 - 10/26/03 11:37 AM (8 years, 7 months ago) |
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Lophophora williamsii v. jourdaniana
Trichocereus pachanoi
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www.911blogger.com
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DazedSol
old hand
Registered: 08/01/01
Posts: 1,230
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Re: Cactus tissue culture. [Re: Una]
#2044693 - 10/26/03 11:42 AM (8 years, 7 months ago) |
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Hey Una, What kind of lighting do you have those jars under while they grow?
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Peace,
Adam
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: DazedSol]
#2046503 - 10/27/03 01:17 AM (8 years, 7 months ago) |
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They grow under simple fluorescent tubes (33/84 cool white)
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www.911blogger.com
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#2056126 - 10/30/03 01:28 AM (8 years, 6 months ago) |
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Yesterday i cut up the newly formed shoots. They had been growing for 2 months. Each shoot yielded between 2-4 new sections that were set up on fresh medium. This gave a mean multiplication factor of 7.
If further multiplication goes with that same number in one year 7^5 plants could be obtained. Not 7^6 because the last cycle the plants can not be cut up but should be rooted in a different medium. Still this would mean 16807 plants in one year starting with one explant. Now THAT is rapid multiplication 
Cytokinin concentration seems to influence number of shoots and number of ribs per shoot (higher -> more)
Here's two pics:
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www.911blogger.com
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stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 2 years, 2 months
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Re: Cactus tissue culture. [Re: Una]
#2056426 - 10/30/03 05:28 AM (8 years, 6 months ago) |
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And growth would probably be faster if after first initial growth, change lightning into something more powerfull light HPS/MH?[right?]
Peace..
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Probably not, the atmosphere within the jars does not allow for much assimilation. The medium contains sucrose to supply the plantlets with energy.
The shoots will also develop in the dark but they will remain white/yellow.
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www.911blogger.com
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,609
Loc: Rigel 7
Last seen: 9 months, 28 days
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Re: Cactus tissue culture. [Re: Una]
#2056583 - 10/30/03 07:27 AM (8 years, 6 months ago) |
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>>If further multiplication goes with that same number in one year 7^5 plants could be obtained. Not 7^6 because the last cycle the plants can not be cut up but should be rooted in a different medium. Still this would mean 16807 plants in one year starting with one explant. Now THAT is rapid multiplication
WOW! 
>>Cytokinin concentration seems to influence number of shoots and number of ribs per shoot (higher -> more)
Rather interesting that it affected number of ribs.
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felixhigh
KIA
Registered: 06/24/01
Posts: 7,533
Loc: Ly
Last seen: 4 days, 17 hours
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Re: Cactus tissue culture. [Re: neuro]
#2056798 - 10/30/03 09:30 AM (8 years, 6 months ago) |
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una, thats it, you've crossed the barrier between the healthy and the insane, please do society a favour and go to intern yourself on a mental hospital.
you leave me without words. it's a little boring to repeat but:
AMAZING!!!
FH
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stonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666
Last seen: 2 years, 2 months
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Re: Cactus tissue culture. [Re: felixhigh]
#2059435 - 10/30/03 11:17 PM (8 years, 6 months ago) |
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Yep..this work is just AMAZING..and very very interesting to follow..
great work UNA..
peace..
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#2102072 - 11/13/03 01:14 PM (8 years, 6 months ago) |
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I just received a beautiful variegated Tr. peruvianus and i couldn't resist setting up some new explants. We'll see what comes out, variegated, yellow or green shoots...
Here's the tip of the plant:
Surface sterilisation of the tip in household bleach on a magnetic stirrer plate. This is followed by three rinses with sterile water.
The tip is cut into sections.
And these are placed on the medium.
Once finished the jars are taped with breathable bandage tape. And yes, those are urine sample jars 
Here's some Lophophora jourdaniana
And some Lophphora williamsii seedlings
My pictures of the san pedro failed but they are developing new shoots on almost all areoles. Multiplication can be even faster than those 16,000 per year!
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www.911blogger.com
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Ekstaza
stranger thanmost
Registered: 04/10/03
Posts: 4,315
Loc: Around the corner
Last seen: 3 months, 1 day
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Re: Cactus tissue culture. [Re: Una]
#2105249 - 11/14/03 06:23 AM (8 years, 6 months ago) |
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Stupendous. I love to see people with great intellect do great things. Una, you are a credit to the human race.
Wonderful
Amazing
Terrific
Outstanding
Yea, I guess that's enough
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YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.
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