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 Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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 Cactus tissue culture.
#1932583 - 09/19/03 01:32 PM (6 years, 1 month ago) |
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Here's my first success.
San pedro developing new buds in vitro 
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 neuro
Phytophiliac
Registered: 08/10/99
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Re: Cactus tissue culture. [Re: Una]
#1932590 - 09/19/03 01:33 PM (6 years, 1 month ago) |
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what's your medium and hormone content and ratio?
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: neuro]
#1932650 - 09/19/03 01:44 PM (6 years, 1 month ago) |
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I can not reveal the recipe but if you are interested in cactus tissue culture this document will give some very usefull tips.
http://plant-tc.coafes.umn.edu/listserv/2000/log0010/msg00077.html
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www.911blogger.com
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neuro
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Re: Cactus tissue culture. [Re: Una]
#1932680 - 09/19/03 01:50 PM (6 years, 1 month ago) |
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heh i already know how to do cultures and such... back in school i had this shitty lab every tuesday afternoon and all we did was make cultures.. i was just curious as to your recipe...but if mum's the word, then mum's the word 
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DazedSol
old hand
Registered: 08/01/01
Posts: 1,230
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Re: Cactus tissue culture. [Re: Una]
#1933189 - 09/19/03 04:47 PM (6 years, 1 month ago) |
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Very fucken cool Una!!!
Please keep us updated on your progress.......
This is on my (really long)list of things to do(different, harder to acquire cacti though)......
anywho.
Hehe, I was gonna ask the same thing as neuro but figured the request would be shot down  With good reason though,..there is a lot of money to made on successful culture formula's ....plus all the time done experimenting and all.........thats alot to just give away
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Peace,
Adam
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theshiftingwalls
Divine state
Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
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Re: Cactus tissue culture. [Re: DazedSol]
#1933403 - 09/19/03 06:12 PM (6 years, 1 month ago) |
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Give us the secrect... Or thats just fucked...
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theshiftingwalls
Divine state
Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
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Malt extract or agar?
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neuro
Phytophiliac
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LOL! Not quite!
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felixhigh
stunt
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Re: Cactus tissue culture. [Re: neuro]
#1933797 - 09/19/03 09:41 PM (6 years, 1 month ago) |
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hehehehe
yeah, MEA, then you insert the Cube spores into the mix and there you have a shroomocactus psychodelicus. =)
very very cool UNA!
could you guys please give us more good links on cactus tissue culture? should i treat them like any other plant then (regarding substrate)?
Edited by felixhigh (09/20/03 01:28 AM)
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: felixhigh]
#1935653 - 09/20/03 02:21 PM (6 years, 1 month ago) |
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Of course the medium is not MEA.... That's for fungal growth not for plant growth (although malt extraxt is sometimes added to tissue culture media as an 'undefined' growth factor).
The cactus medium is based on Murashige and Skoog (MS) salts, sugar (sucrose), agar and growth regulators.
I've searched for many hours on the internet for info on TC of cactus but there's not much info out there. The link i posted earlier sums up (practically) all the info that is out there.
Whenever i have the time i will go visit the library of the Agricultural University in Wageningen because i'm sure that many scientific articles have been published about the subject that i can get my hands on there.
As for me not wanting to share the exaxt recipe; This is something we might want to take to a commerical scale (sometimes). In fact i studied horticulture for 4 years and i did my final thesis on the development of plant tissue culture protocols
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www.911blogger.com
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neuro
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Re: Cactus tissue culture. [Re: Una]
#1935877 - 09/20/03 04:03 PM (6 years, 1 month ago) |
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>>As for me not wanting to share the exaxt recipe; This is something we might want to take to a commerical scale (sometimes). In fact i studied horticulture for 4 years and i did my final thesis on the development of plant tissue culture protocols
Ahh so this is a carefully investigated recipe.. i understand now..
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theshiftingwalls
Divine state
Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
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Re: Cactus tissue culture. [Re: neuro]
#1936033 - 09/20/03 05:04 PM (6 years, 1 month ago) |
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Told you he used agar and malt extract.
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Quote:
Told you he used agar and malt extract. Quote:
I use malt extract for the growth of fungi, haven't used it for TC plants yet. Malt extract is regarded as complex organic supplement of which the effects are not completely understood.
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www.911blogger.com
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#1938225 - 09/21/03 03:29 PM (6 years, 1 month ago) |
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Here's some more pics.....
more pachanoi
Lophophora developing a new pup
Pereskiopsis rooting stage
oh here's some Salvia divinorum (not a cactus i know)
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www.911blogger.com
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neuro
Phytophiliac
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Posts: 6,604
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Re: Cactus tissue culture. [Re: Una]
#1938533 - 09/21/03 06:17 PM (6 years, 1 month ago) |
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looks good una..
have you tried producing a callus then growing shoots or roots off the callus in a separate culture?
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ISH
PsilocybianTranslationServices
Registered: 03/02/02
Posts: 784
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Re: Cactus tissue culture. [Re: neuro]
#1938571 - 09/21/03 06:33 PM (6 years, 1 month ago) |
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Bad Ass, I'm very interested in seeing your progress.
ISH
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Join The Shroomery Folding @ Home Team!
I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
- Terence McKenna, Archaic Revival
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Agarico
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 Re: Cactus tissue culture. [Re: Una]
#1938577 - 09/21/03 06:36 PM (6 years, 1 month ago) |
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Is it possible to do this without aureoles? Just tissue? 
Cloning Salvia that way looks like everything else than a productive method would 
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WHITE IS THE COLOUR OF THE WIND.
take a look at my sculptures updated 13sept.
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Effed
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Re: Cactus tissue culture. [Re: Una]
#1939019 - 09/21/03 09:34 PM (6 years, 1 month ago) |
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Una, you are a true innovator. 
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 I'll be a new man tomorrow...
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psilocybe cubensis
Blood, guts, andbroken teeth.
Registered: 08/09/00
Posts: 1,220
Loc: Lost Angels
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Re: Cactus tissue culture. *DELETED* [Re: Effed]
#1939068 - 09/21/03 09:58 PM (6 years, 1 month ago) |
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Post deleted by psilocybe cubensis
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Openminded
Dicotyledon
Registered: 08/28/03
Posts: 657
Loc: England.
Last seen: 1 year, 6 months
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Very, very nice. I've always been fascinated by this sort of tissue culture. I love the way you can simply slice off a bit of something and it will grow into a whole new plant. Well, not quite that simple in practice...
I love growing things, but feel more at home in the lab than in the garden, so this would be a perfect hobby for me!
Please, do keep us informed about your progress. How long did it take for the new shoots to form?
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