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 abductee
Time
 
Registered: 05/07/15
Posts: 2,224
Loc: Canada
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When you slowly bring it to pressure do you start at medium for 10, then medium high, then high?, how slow is slow?
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insanemike
Registered: 02/23/14
Posts: 4,272
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 Re: Pasty Agar Tek  [Re: abductee]
#21877511 - 06/30/15 10:50 AM (8 years, 9 months ago) |
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Quote:
abductee said:
When I read that it grows really well after forest fires and old apple orchards I thought it had somerhing to do with the soil, would it be more acidic? Higher levels of some ph balance, more light, I dont think just adding any root system would help.
Actually the ash would bring the ph up and make the soil more alkaline.
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abductee
Time
Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Yea, so thats probably what it favours 
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Grey
⇜ ✯ ⇝
Registered: 11/06/14
Posts: 6,223
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Re: Pasty Agar Tek [Re: abductee]
#21877749 - 06/30/15 11:52 AM (8 years, 9 months ago) |
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I get the water in the PC boiling on 8 (electric stove) put the lid on (let it vent) and drop it to 7 maybe 6 until it gets to pressure.
Stoves are different though. Once mine is up to pressure/temp, I can keep my PC idling at 15 psi with my stove set to just over the 3 Mark
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  AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Pasty Agar Tek [Re: Grey]
#21878128 - 06/30/15 01:26 PM (8 years, 9 months ago) |
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Quote:
Grey said:
I get the water in the PC boiling on 8 (electric stove) put the lid on (let it vent) and drop it to 7 maybe 6 until it gets to pressure.
Stoves are different though. Once mine is up to pressure/temp, I can keep my PC idling at 15 psi with my stove set to just over the 3 Mark
Hahaha. What a coincidence I have the same setting.
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Yeah I wouldn't wanna try to put the lid on my AA after it's already boiling... I guess depending on how long.
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abductee
Time
Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: Grey]
#21880143 - 06/30/15 08:46 PM (8 years, 9 months ago) |
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Im sure it worked fine, in still getting used to pressure cookers and over thought bringing it up slowly.lol. Got some new plates to lay some spores on. It's going to be my first time working with prints and will be going over some good tutorials I saw around here. it's late and not going to inoculate now, but excited about tomorrow
Edited by abductee (07/01/15 04:19 PM)
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abductee
Time
Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: abductee]
#21909043 - 07/07/15 10:16 AM (8 years, 9 months ago) |
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Finally got some clean growth going on  Thanks to this tek and the people contributing info
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Pastywhyte 
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Re: Pasty Agar Tek [Re: abductee]
#21918551 - 07/09/15 09:10 AM (8 years, 9 months ago) |
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Great stuff man. You're gonna love agar 
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eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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had to add a shelf to the storage.
thought you would like this one pasty.
 
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Indeed I do, that's some sweet culture action ya got going on
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eatyualive
Eat's You Alive :)
Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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yeah i only use an exacto blade for culture work. i have 50 blades left as replacements. i used that same 1st blade for 8 years. i recently replaced it last week.
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Thoky.ykoht
Registered: 10/08/13
Posts: 399
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Do you think these plates from 4/20 are still salvageable?
They've been in my fridge, in ziplocks with two pieces of micropore tape over the hole in the lid.
I don't want to waste time cooking more up if I'm dead in the water.
      
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 PenisPornProducer
It's What's for Dinner!
Registered: 07/06/15
Posts: 19
Last seen: 8 years, 8 months
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If I was in a position I needed to use those I would first take a transfer to a new agar plate.
Its hard to tell from the pictures exactly how much growth those plates have though.
Edited by PenisPornProducer (07/14/15 10:19 AM)
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insanemike
Registered: 02/23/14
Posts: 4,272
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Were them plates refrigerated after full colonization? I'm asking because they look pretty healthy for being so old. If I leave mine out for 4-6 weeks after colonization, they shrivel and look very unhealthy. But after transfer, they become vigorous again.
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lovesquare
Love²
Registered: 06/04/15
Posts: 556
Last seen: 8 years, 6 months
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+5 pasty, thanks for your time and dedication to the community, just marking this so I can refer to it when I start agar for the first time for my edibles/medicinals this fall. 
--------------------
If you go down round the bend in the river,
You're gonna find a few changes been going down there.
If you go down to the gas-powered flatland,
Where most of the people just think that they're free,
Remember the peace that you had on the mountain,
Come back to the love that you had here with me...
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Thoky.ykoht
Registered: 10/08/13
Posts: 399
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I tossed them in the fridge on 5/4 so they had a couple weeks to grow out.
I planned to transfer them.
I thought they looked pretty healthy for as old as they were, but someone said they looked "dry" so I figured I'd ask around before jumping the gun.
I was curious if I can get some jars going with whatever is leftover or if its just to old and i should wait for the transfers to grow out?
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insanemike
Registered: 02/23/14
Posts: 4,272
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I would not drop any of those into grain jars. I would instead opt to transfer before knocking anything up, just to be on the safe side.
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kushroom
Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 6 months, 27 days
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So i think imma try my first real attempt with agar, im not using anything fancy just some baby food jars, if i put a hole in the top nd filled with polyfil would that work out?
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:/
   
All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Pasty Agar Tek [Re: kushroom]
#21948893 - 07/15/15 06:51 PM (8 years, 8 months ago) |
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Quote:
kushroom said:
So i think imma try my first real attempt with agar, im not using anything fancy just some baby food jars, if i put a hole in the top nd filled with polyfil would that work out?
Glad mini rounds are cheap and easily obtainable. There is nothing wrong with using glass but with plastic you can loosen up the agar before you even open the lid and drop it into a grain jar or liquid medium without having to use any tools which eliminates atleast one possible contam vector.
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