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Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Paul Stamets, Rye Grain

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Offlinebjanuszewski
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shaking spawn jars
    #19030777 - 10/25/13 02:06 PM (8 months, 23 days ago)

I had two quart jars of rye berries w/ two agar wedges of oysters in them. they started colonizing extremely fast, after they where colonized fairly well I gave em a shaking. now after close to a good month there is no sign of colonization, its as if I killed the mycelium. I had to give them a good shaking as the berries just weren't coming undone, but I believe that's expected w/ colonized berries. im thinking next time of just leaving the jars alone getting a nice white colonization and just spawn to bigger subs with that. anyone else have this issue or just not shake their spawn jars?


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Invisibleliamtheloser
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Re: shaking spawn jars [Re: bjanuszewski]
    #19030857 - 10/25/13 02:26 PM (8 months, 23 days ago)

Sounds like maybe you let them go too long before shaking.

I shake when the quart jar has a golfball size chunk of mycelium. It shouldn't be difficult to break apart.

But also,  I don't think it would outright kill the mycelium. Maybe you had a contaminate in the jar and when you shook it,  the mycelium couldn't fight it off.


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Offlinebjanuszewski
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Re: shaking spawn jars [Re: liamtheloser]
    #19031625 - 10/25/13 05:19 PM (8 months, 23 days ago)

was just reading you should shake @ 30 % colonized. I thought once jar had around full colonization was when you shook it :confused:


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OfflineJeff
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Re: shaking spawn jars [Re: bjanuszewski]
    #19031712 - 10/25/13 05:34 PM (8 months, 23 days ago)

I think liam is on point here.  It should have recovered even if you let it go too far if there were no contaminates present.


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Re: shaking spawn jars [Re: Jeff]
    #19034816 - 10/26/13 11:44 AM (8 months, 22 days ago)

My opinion would be a contam as well. You should see recovery in 24 hours if your culture is good..


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OfflineAmanita virosa
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Re: shaking spawn jars [Re: nanncee]
    #19037348 - 10/26/13 09:36 PM (8 months, 22 days ago)

Agreed. Classic test for bacterial contam. Shaking will always favor the unicellular bacteria over the filamentous mycelium. Liquid culture is prone to contam for exactly the same reason. Favors the bacteria.


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OfflineForrester
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Re: shaking spawn jars [Re: Amanita virosa]
    #19037488 - 10/26/13 09:56 PM (8 months, 22 days ago)

Quote:

Amanita virosa said:
Shaking will always favor the unicellular bacteria over the filamentous mycelium.




That's weird, I have a contaminated syringe I've done all my turkey tail grows with.  Bacteria usually starts to take hold at around 30% colonization.  I just shake the hell out of the jar and by the time the mycelium recovers there's no longer any signs of bacteria, it finishes colonizing and grows fine when spawned.  Of course that's turkey tails they're pretty tough.

Try to put a drop on agar though and the bacteria dominates almost immediately, TT never had a chance.  :shrug:


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Offlinedeadmandave
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Re: shaking spawn jars [Re: Forrester]
    #19038297 - 10/27/13 12:21 AM (8 months, 22 days ago)

forrester, maybe you can take a colonized grain to agar and sector a clean bit of mycelium at that point. Or try one of Stamets recommendations, run the mycelium through a piece of straw in a slant, by the time it gets to the end it may have outrun the bacteria and you can place it on agar and get a clean culture.

btw if your plates are full of condensation you may want to try and let the agar cool a bit longer before pouring and keep them stacked while cooling.

On the OP, I dont shake my jars any more. I dont find shaking to increase colonization speeds much at all and it feels like unnecessary roughness during the early phase of cultivation.  I use four bits of agar when i inoculate and after the initial shake i let it colonize at it's own pace.


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OfflineForrester
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Re: shaking spawn jars [Re: deadmandave]
    #19039549 - 10/27/13 09:24 AM (8 months, 21 days ago)

Yeah that's a good idea I think I'm going to try the piece of colonized grain to agar route, it should work. 
I pour my plates before PC'ing, in those little flat ball jars, so not too much I can do about condensation until I get a flow hood and can start pouring after.


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OfflineRogerRabbitM
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Re: shaking spawn jars [Re: Forrester]
    #19050872 - 10/29/13 10:04 AM (8 months, 19 days ago)

The bottom line is if you shake and it doesn't recover, it was contaminated.
RR


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Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood, Paul Stamets, Rye Grain

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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