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Offlinehorusiscalling
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Collecting spores from wild mushrooms
    #18817066 - 09/09/13 12:29 AM (11 months, 12 days ago)

has anyone had success collecting spores from the wild and germinating them without contam? I'm wondering if my prints are clean enough. any tips on the subject are appreciated :cool:

also the mushrooms im trying to print have long been "unvieled"


Edited by horusiscalling (09/09/13 12:39 AM)


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InvisibleGanzig
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Re: Collecting spores from wild mushrooms [Re: horusiscalling]
    #18817123 - 09/09/13 12:48 AM (11 months, 12 days ago)

You should make a clone. You can totally make a culture from a wild
print but making a clone, in my opinion, is easier.


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Offlinedodeski
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Re: Collecting spores from wild mushrooms [Re: Ganzig]
    #18817352 - 09/09/13 01:41 AM (11 months, 12 days ago)

I have not tried. Search for PF tek on here or use your Google substitute. I If you take your print on foil I hear it's easier to transfer spores to your dish. I then believe you have to chase your contaminate out. you do this by cutting out pieces of your culture that are not contaminated and re-culturing them in a new sterile dish. You then keep doing this until you come to a culture that is clean.


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Invisibleinski
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Re: Collecting spores from wild mushrooms [Re: Ganzig]
    #18817529 - 09/09/13 02:53 AM (11 months, 12 days ago)

Quote:

Ganzig said:
You should make a clone. You can totally make a culture from a wild
print but making a clone, in my opinion, is easier.



In my experience cloning wild collected mushrooms is more difficult than making spore prints and starting cultures from them, more often than not you will get bacterial contamination with tissue culture.

The best method I have found is to take a small piece of fresh gill fragment using sterile tweezers and swipe it on an agar plate, do the same with at least three or four dishes, it will be essential to do a few transfers to isolate a pure culture, I have been able to start good cultures with all species I have tried this with.

I would only attempt tissue culture if the fungus in question is sterile or almost sterile.


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InvisibleGanzig
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Re: Collecting spores from wild mushrooms [Re: inski]
    #18817553 - 09/09/13 03:03 AM (11 months, 12 days ago)

I must have had some bad luck. I have had a hard time with spore prints compared
to clones. I have had great luck with tearing a mushroom open and pulling
tissue from inside and getting a clean culture.

I would trust what inski says. He has a lot more cultivation work under belt
than I do.


--------------------
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Edited by Ganzig (09/09/13 03:07 AM)


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InvisibleDCart
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Re: Collecting spores from wild mushrooms [Re: Ganzig]
    #18818076 - 09/09/13 09:51 AM (11 months, 12 days ago)

I have made prints on tin foil and wax paper and then transfered the spores to agar. From there you grow out the colony and transfer healthy, clean, fast growin mycelium to another dish.

I've tried cloning wild oyster mushrooms and had pretty bad luck. I tore the stipe in half and took some tissue from the inner stipe and put it on agar.... Yeah it was a green nasty mess within a week or so.

What kind of mushroom are you trying to culture?


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OfflineMacMerdinS
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Re: Collecting spores from wild mushrooms [Re: DCart]
    #18818309 - 09/09/13 11:43 AM (11 months, 12 days ago)

I have found that with woodlovers, using a modified pf tek with aspen snake bedding works well.  Unless the prints were taken in a moldy basement with no oversight as to any clean technique (even then only about 20% contamed).

Psilocybe ovoideocystidiata



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Edited by MacMerdin (09/09/13 11:45 AM)


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Offlinemahniti
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Re: Collecting spores from wild mushrooms [Re: MacMerdin]
    #18818643 - 09/09/13 01:52 PM (11 months, 12 days ago)

my friend managed to do that with agrocybe aegerita on poplar sawdust.


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OfflineRiparianZoneJunky
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Re: Collecting spores from wild mushrooms [Re: mahniti]
    #18818733 - 09/09/13 02:25 PM (11 months, 12 days ago)

As long as the specimen is fresh and relatively clean, I've had good luck cloning as well as taking prints, it's really only when the specimen is old, manky, half rotten and bug eaten that you get problems, and at that point both a spore print as well as a clone are going to be problematic.  :lol:


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Offlinehorusiscalling
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Re: Collecting spores from wild mushrooms [Re: DCart]
    #18825086 - 09/10/13 10:09 PM (11 months, 11 days ago)

all i can say is its not p. cubensis. :cool:

so how to i transfer the agar culture to a grain spawn sterilly? I have a glove box.


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OfflineRiparianZoneJunky
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Re: Collecting spores from wild mushrooms [Re: RiparianZoneJunky]
    #18825654 - 09/11/13 12:16 AM (11 months, 10 days ago)

Just sterilize the jar with alcohol, in the box, flame sterilize the scalpel outside of the box, cut a small square of mycelium from the leading edge, pick it up with the scalpel and pop it off into the jar real quick with minimal time open. 


--------------------
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Edited by RiparianZoneJunky (09/11/13 12:18 AM)


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InvisibleGanzig
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Re: Collecting spores from wild mushrooms [Re: RiparianZoneJunky]
    #18825662 - 09/11/13 12:18 AM (11 months, 10 days ago)

Agreed.

Been having bacterial problems with my flowhood recently. But more recently I
fixed it! Woot. Been having a great time doing plate work.

speaking of, I have a bunch of pink oyster that I don't mind sharing.


--------------------
I must keep reminding myself of this.
I must keep reminding myself of this. I must keep reminding myself of this.
I must keep reminding myself of this.


I'm a Canadian stuck in an American's body.


MycosenseMushrooms
Check out our cultures and kits!


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