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horusiscalling
Partner
Registered: 08/17/13
Posts: 111
Last seen: 10 years, 6 months
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Collecting spores from wild mushrooms
#18817066 - 09/08/13 10:29 PM (10 years, 7 months ago) |
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has anyone had success collecting spores from the wild and germinating them without contam? I'm wondering if my prints are clean enough. any tips on the subject are appreciated
also the mushrooms im trying to print have long been "unvieled"
Edited by horusiscalling (09/08/13 10:39 PM)
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Ganzig
It's for the street cred
Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Collecting spores from wild mushrooms [Re: horusiscalling]
#18817123 - 09/08/13 10:48 PM (10 years, 7 months ago) |
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You should make a clone. You can totally make a culture from a wild print but making a clone, in my opinion, is easier.
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dodeski
Student of liff
Registered: 11/30/08
Posts: 576
Loc: OR
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Re: Collecting spores from wild mushrooms [Re: Ganzig]
#18817352 - 09/08/13 11:41 PM (10 years, 7 months ago) |
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I have not tried. Search for PF tek on here or use your Google substitute. I If you take your print on foil I hear it's easier to transfer spores to your dish. I then believe you have to chase your contaminate out. you do this by cutting out pieces of your culture that are not contaminated and re-culturing them in a new sterile dish. You then keep doing this until you come to a culture that is clean.
-------------------- "People use the word "natural" ... What is natural to me are these botanical species which interact directly with the nervous system. What I consider artificial is 4 years at Harvard, and the Bible, and Saint Patrick's cathedral, and the Sunday school teachings." -Timothy Leary “You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness.” ― Terence McKenna "In defying the authority we become the authorities" - Unknown
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inski
Cortinariologist
Registered: 02/28/06
Posts: 5,776
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Re: Collecting spores from wild mushrooms [Re: Ganzig]
#18817529 - 09/09/13 12:53 AM (10 years, 7 months ago) |
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Quote:
Ganzig said: You should make a clone. You can totally make a culture from a wild print but making a clone, in my opinion, is easier.
In my experience cloning wild collected mushrooms is more difficult than making spore prints and starting cultures from them, more often than not you will get bacterial contamination with tissue culture.
The best method I have found is to take a small piece of fresh gill fragment using sterile tweezers and swipe it on an agar plate, do the same with at least three or four dishes, it will be essential to do a few transfers to isolate a pure culture, I have been able to start good cultures with all species I have tried this with.
I would only attempt tissue culture if the fungus in question is sterile or almost sterile.
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Ganzig
It's for the street cred
Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Collecting spores from wild mushrooms [Re: inski]
#18817553 - 09/09/13 01:03 AM (10 years, 7 months ago) |
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I must have had some bad luck. I have had a hard time with spore prints compared to clones. I have had great luck with tearing a mushroom open and pulling tissue from inside and getting a clean culture.
I would trust what inski says. He has a lot more cultivation work under belt than I do.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
Edited by Ganzig (09/09/13 01:07 AM)
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DCart
Strangest
Registered: 06/18/13
Posts: 530
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Re: Collecting spores from wild mushrooms [Re: Ganzig]
#18818076 - 09/09/13 07:51 AM (10 years, 7 months ago) |
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I have made prints on tin foil and wax paper and then transfered the spores to agar. From there you grow out the colony and transfer healthy, clean, fast growin mycelium to another dish.
I've tried cloning wild oyster mushrooms and had pretty bad luck. I tore the stipe in half and took some tissue from the inner stipe and put it on agar.... Yeah it was a green nasty mess within a week or so.
What kind of mushroom are you trying to culture?
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MacMerdin
Hunter
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Re: Collecting spores from wild mushrooms [Re: DCart]
#18818309 - 09/09/13 09:43 AM (10 years, 7 months ago) |
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I have found that with woodlovers, using a modified pf tek with aspen snake bedding works well. Unless the prints were taken in a moldy basement with no oversight as to any clean technique (even then only about 20% contamed).
Psilocybe ovoideocystidiata
Edited by MacMerdin (09/09/13 09:45 AM)
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mahniti
Stranger
Registered: 10/22/12
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Loc: south europe
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Re: Collecting spores from wild mushrooms [Re: MacMerdin]
#18818643 - 09/09/13 11:52 AM (10 years, 7 months ago) |
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my friend managed to do that with agrocybe aegerita on poplar sawdust.
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RiparianZoneJunky
hunter/gatherer
Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 8 months
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Re: Collecting spores from wild mushrooms [Re: mahniti]
#18818733 - 09/09/13 12:25 PM (10 years, 7 months ago) |
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As long as the specimen is fresh and relatively clean, I've had good luck cloning as well as taking prints, it's really only when the specimen is old, manky, half rotten and bug eaten that you get problems, and at that point both a spore print as well as a clone are going to be problematic.
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horusiscalling
Partner
Registered: 08/17/13
Posts: 111
Last seen: 10 years, 6 months
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Re: Collecting spores from wild mushrooms [Re: DCart]
#18825086 - 09/10/13 08:09 PM (10 years, 7 months ago) |
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all i can say is its not p. cubensis.
so how to i transfer the agar culture to a grain spawn sterilly? I have a glove box.
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RiparianZoneJunky
hunter/gatherer
Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 8 months
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Just sterilize the jar with alcohol, in the box, flame sterilize the scalpel outside of the box, cut a small square of mycelium from the leading edge, pick it up with the scalpel and pop it off into the jar real quick with minimal time open.
Edited by RiparianZoneJunky (09/10/13 10:18 PM)
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Ganzig
It's for the street cred
Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Agreed.
Been having bacterial problems with my flowhood recently. But more recently I fixed it! Woot. Been having a great time doing plate work.
speaking of, I have a bunch of pink oyster that I don't mind sharing.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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