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OfflineET80s
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Registered: 01/06/13
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2nd attempt at monotubs w. contams. is it the grains maybe?
    #18811301 - 09/07/13 11:50 AM (10 years, 6 months ago)

this is my 2nd failed attemp useing monotubs the first was a disaster. i know it. i even post something about it tht dident get any replys (i assume its fairly obvious i was ignoring teks ide read) so heres the most recent i followed some of TLs things along with frank horrigans substrate recipe and pasteurizing process using a 1/1 ratio. i have contams in tubs again and im wondering if maybe its hidden in my grains or is it possible that the Mycelium gets weak and this leads to contams. heres some pics of whats happened so far.

And 12 days later it looks like this.
heres the other one

these are not the grains i used but they looked the exact same just fully colonized these were spore to lc to grains and a series of g2gs. im working with agar again as of last week so well see how those grains turn out soon.


if these look a little odd would these be more acceptable for a monotub i have 20 jars and i was just going to case them and throw um in a sgfc because i find it hard to break them up after theyve been left this long(4weeks)


any info would be great ive put alot of time and $ into this hobby itd be nice to see some results. after all ,halloween is just around the corner:)


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OfflinePsuper
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Registered: 02/12/08
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Re: 2nd attempt at monotubs w. contams. is it the grains maybe? [Re: ET80s]
    #18811368 - 09/07/13 12:08 PM (10 years, 6 months ago)

How long are you letting your fully colonized jars sit around?

What is your exact pastuerization  process?


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OfflineET80s
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Re: 2nd attempt at monotubs w. contams. is it the grains maybe? [Re: Psuper]
    #18811389 - 09/07/13 12:17 PM (10 years, 6 months ago)

as soon as they look fully colonized on the outside (after 2 shakes) one at 5% and another @ 20% i give them another shake and let them sit a day or 2 and use them. some i have let sit around longer like the ones pictured (4weeks) but i havent used those grains for tubs.
i pasteurized using the RRs method (into the jars then the kettle for an hour between 140 and 160c. i also make my grains using his methods they come out nice every time.


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OfflineDeadPhan
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Re: 2nd attempt at monotubs w. contams. is it the grains maybe? [Re: ET80s]
    #18811459 - 09/07/13 12:40 PM (10 years, 6 months ago)

I might be wrong but that doesn't look like mycelium to me. Unless they are gourmets it looks quite cottony.  I'm still learning but seems a lil suspect to me.


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OfflineET80s
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Re: 2nd attempt at monotubs w. contams. is it the grains maybe? [Re: DeadPhan]
    #18811624 - 09/07/13 01:31 PM (10 years, 6 months ago)

im just gonna add this to here now, how does this agar look alright this is a 3rd transfer and  what im working with now, "golden mammoth strain. i threw it on some grains last week and its doing pretty good almost ready for the first shake.


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InvisibleSpitballJedi
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Re: 2nd attempt at monotubs w. contams. is it the grains maybe? [Re: DeadPhan]
    #18811896 - 09/07/13 03:04 PM (10 years, 6 months ago)

Quote:

DeadPhan said:
I might be wrong but that doesn't look like mycelium to me. Unless they are gourmets it looks quite cottony.  I'm still learning but seems a lil suspect to me.



exactly what I was thinking.


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OfflineET80s
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Re: 2nd attempt at monotubs w. contams. is it the grains maybe? [Re: SpitballJedi]
    #18816166 - 09/08/13 06:04 PM (10 years, 6 months ago)

i was reading the mushroom cultivator about agar culture, theres a place in there where it says that when mycelium get old its said to be senescing and changes from a rhizomorphic to cottony, i thought maybe ive just played with the same culture for too long and thats what happened. Better then thinking ive just been playing with a contaminated culture for the last 6 months.


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