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 Stropharis
Registered: 04/02/07
Posts: 1,854
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 Re: Stro's Cleaning and Isolating On Agar  [Re: cronicr]
#19673668 - 03/09/14 09:40 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
Stropharis said:
Just stopping by, I'm hoping to be back at it in March, we will see.
it's march, i didn't forget lol! bumpity bump stropharis where ya at!
I LOL'D! I'm in the shadows Breh! Lately I can't help myself though and fungus is on the mind.
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 cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle 
Last seen: 2 years, 2 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
#19673861 - 03/09/14 10:24 PM (10 years, 1 month ago) |
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   It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mdahmer
Aloysius devadander abercrombie
Registered: 04/05/14
Posts: 2,516
Last seen: 6 years, 8 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
#19876913 - 04/21/14 06:33 AM (9 years, 11 months ago) |
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I know this is old but I want ithe thread, thanks man nice pics 
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cArcace-x
YeAh!!
Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
#20001359 - 05/17/14 10:22 AM (9 years, 10 months ago) |
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wow man nice write-up!
i just jumped into agar and you've explained it very well
THAnx A10T
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Keyser Soze
Registered: 11/07/11
Posts: 36
Loc: Canada
Last seen: 9 years, 3 months
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Re: Stro's Cleaning and Isolating On Agar [Re: cArcace-x]
#20001543 - 05/17/14 11:26 AM (9 years, 10 months ago) |
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WOW!!! Very informative & thorough thread...Thank You Stro for taking the time.
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"A spook story that criminals tell their kids at night."
Roger "Verbal" Kint
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magicMerlin
Registered: 01/26/12
Posts: 617
Loc: Toronto
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Re: Stro's Cleaning and Isolating On Agar [Re: Keyser Soze]
#20047148 - 05/27/14 02:13 PM (9 years, 10 months ago) |
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Awesome write up Stro!
Would it be best to put spores, a pin, or the inside of a fruit body onto agar? I haven't found much info on this and it's still a little confusing to me.
Thank you!
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My favorite threads
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Stropharis
Registered: 04/02/07
Posts: 1,854
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Re: Stro's Cleaning and Isolating On Agar [Re: magicMerlin] 1
#20057794 - 05/29/14 07:03 PM (9 years, 10 months ago) |
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Quote:
magicMerlin said:
Awesome write up Stro!
Would it be best to put spores, a pin, or the inside of a fruit body onto agar? I haven't found much info on this and it's still a little confusing to me.
Thank you!
If you already have a multispore grow going and you want to start working with clones and/or monocultures, then it would be best to transfer a live tissue culture to agar. This clone culture would be the same genetically. However, it will more than likely still carry multiple sectors and not be a true isolate, and it also more than likely won't need to be in order to preform well.
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rohr
silly cybin
Registered: 08/08/11
Posts: 384
Loc: TX 
Last seen: 8 years, 9 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Stropharis]
#20058076 - 05/29/14 07:57 PM (9 years, 10 months ago) |
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Thanks, stro
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Knothead
Invisable
Registered: 01/10/14
Posts: 294
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Re: Stro's Cleaning and Isolating On Agar [Re: rohr]
#20066524 - 05/31/14 08:10 PM (9 years, 10 months ago) |
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First of all I want to say THANK YOU, nice write up and I learned that what I thought I understood was wrong. Thanks again for the knowledge.
I have a question:
Is it possible to get a non fruiting strain from and "cloned" isolate?
Edited by Knothead (05/31/14 08:24 PM)
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
#20066538 - 05/31/14 08:13 PM (9 years, 10 months ago) |
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Quote:
Knothead said:
Is it possible to get a non fruiting strain from and isolate?
yes, this is why they need to be tested
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It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilicon
Really Nice Guy
Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
#20066539 - 05/31/14 08:13 PM (9 years, 10 months ago) |
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I hope Stro doesn't mind me jumping in here to answer that, but yes, it's entirely possible. You should check out pussyfart's 10 PE ISOLATES thread to see what the end result of isolation by a method very similar to this one can be.
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Knothead
Invisable
Registered: 01/10/14
Posts: 294
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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
#20066590 - 05/31/14 08:25 PM (9 years, 10 months ago) |
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I edited the question, I meant to say "cloned" isolate. Sorry!
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Psilicon
Really Nice Guy
Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
#20066603 - 05/31/14 08:27 PM (9 years, 10 months ago) |
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Do you mean an isolate from a clone or a clone of an isolate?
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Knothead
Invisable
Registered: 01/10/14
Posts: 294
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Re: Stro's Cleaning and Isolating On Agar [Re: Psilicon]
#20066610 - 05/31/14 08:30 PM (9 years, 10 months ago) |
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Thanks Pussyfart, I read that a while back and didn't catch they where cloned isolates. Not trying to rub yall wrong, sometimes us all farts just don't on catch everything the first time. Thanks for the feedback as always.
Another misconception that I had. Thought I understood and found out I didn't.
Sure it's never happened to you. LOL
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Knothead
Invisable
Registered: 01/10/14
Posts: 294
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Re: Stro's Cleaning and Isolating On Agar [Re: Psilicon]
#20066615 - 05/31/14 08:31 PM (9 years, 10 months ago) |
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I'm referring to an isolate from a clone, Pussfart provided the answer I think.
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Knothead
Invisable
Registered: 01/10/14
Posts: 294
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Re: Stro's Cleaning and Isolating On Agar [Re: Psilicon]
#20066645 - 05/31/14 08:37 PM (9 years, 10 months ago) |
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Sorry VDG, thanks for pointing me to the answer on Pussyfats post! Appreciate the response, confused again.
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
#20066664 - 05/31/14 08:44 PM (9 years, 10 months ago) |
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all isolates even from clones need to be tested, it';s rare to happen but it is possible to get a non fruiter from a clone
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It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Knothead
Invisable
Registered: 01/10/14
Posts: 294
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Re: Stro's Cleaning and Isolating On Agar [Re: cronicr]
#20066724 - 05/31/14 09:02 PM (9 years, 10 months ago) |
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Thanks Chonicr, another misconception I had, haven't experienced it, thought I understood something and found out I was wrong.
Live, pay attention and learn.
Thanks to all!
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Dilated
KB Cubensis Fanaticus
Registered: 03/28/14
Posts: 2,347
Loc: The Ether
Last seen: 3 years, 4 months
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Re: Stro's Cleaning and Isolating On Agar [Re: Knothead]
#20137567 - 06/15/14 11:40 PM (9 years, 9 months ago) |
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This is exactly what I needed as I venture onward into my quest of cloning and isolation. Bravo, very well written and easily digested.
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Stro's Cleaning and Isolating On Agar [Re: Dilated]
#20137779 - 06/16/14 12:41 AM (9 years, 9 months ago) |
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Quote:
Dilated said:
This is exactly what I needed as I venture onward into my quest of cloning and isolation. Bravo, very well written and easily digested.
QFT...im pretty sure this thread alone has helped more ppl than stropharis can imagine...
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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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