|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 months, 5 days
|
10 PE ISOLATES 16
#18219379 - 05/05/13 07:36 PM (10 years, 10 months ago) |
|
|
So I took tissue samples from 3 nice MS PE fruits and placed them on agar.
After a few transfers, I had 10 plates that looked like this.
I then cut a nice wedge from each dish and inoculated 10 master grain jars with them.
Took about 2.5 weeks for full colonization of all jars, shaking each jar once at 20-30%.
Once they were all fully colonized, I expanded the mycellium via G2G(Grain to Grain) to 9 quarts each.
Grains were WBS(Wild Bird Seed) rinsed and soaked in hot tap water for 24 hours, then strained for 20 minutes, loaded into quart jars and PCed for 100 minutes at 15psi.
Once all 90 were fully colonized, 8 quarts from each batch/culture(1-10) were spawned to 1 brick of coir(650g) and 3 quarts of verm following Damion5050's Bucket Tek.
I used 5.5qts of water in my preparation. This equals out to 4qts for the brick of coir, and .5qts for each quart of verm.
They were spawned in monotubs and were all fully colonized in 10 days, and then fruited.(No consolidation)
Spawn was mixed evenly with the substrate with no top layer and have all shown just about equal colonizations speeds.
TUB - ISOLATE #1 FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17(HARVEST)
FRUITING DAY 20 FRUITING DAY 22 FRUITING DAY 23 (HARVEST 8.5oz DRY)
TUB - ISOLATE #2 - NOTHING AFTER A MONTH - AINT NOBODY GOT TIME FOR THAT! FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17
FRUITING DAY 20 FRUITING DAY 22
TUB - ISOLATE #3 FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17(HARVEST)
FRUITING DAY 20 FRUITING DAY 22 FRUITING DAY 23 (HARVEST 6oz DRY)
TUB - ISOLATE #4 - UNFORTUNATLEY LOST TO TRICH - FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11
TUB - ISOLATE #5 - NOTHING AFTER A MONTH - AINT NOBODY GOT TIME FOR THAT! FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17
FRUITING DAY 20 FRUITING DAY 22
TUB - ISOLATE #6 FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17(HARVEST)
FRUITING DAY 20 FRUITING DAY 22 FRUITING DAY 23 (HARVEST 5.5oz DRY)
TUB - ISOLATE #7 - HIT WITH TRICH/COBWEB - ISOLATED TO FINISH FLUSH FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17(HARVEST)
FRUITING DAY 20 FRUITING DAY 22 FRUITING DAY 23 (HARVEST 5.75oz DRY)
TUB - ISOLATE #8 - HIT WITH TRICH AFTER 3 WEEKS - TOSSED FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17
FRUITING DAY 20 FRUITING DAY 22
TUB - ISOLATE #9 FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17(HARVEST)
FRUITING DAY 20 FRUITING DAY 22 FRUITING DAY 26 (HARVEST 7.3oz DRY)
TUB - ISOLATE #10 FRUITING DAY 1 FRUITING DAY 8 FRUITING DAY 11 FRUITING DAY 15 FRUITING DAY 17(HARVEST)
FRUITING DAY 20 FRUITING DAY 22 FRUITING DAY 26 (HARVEST 4.4oz DRY)
RANDOM PICS
First Flush Totals:
Blobs 4.00oz Tub #1 8.50oz Tub #3 6.00oz Tub #6 5.50oz Tub #7 5.75oz Tub #9 7.30oz Tub #10 4.40oz Total = 41.45 Ounces of Cock!
Edited by PussyFart (05/17/13 02:48 PM)
|
cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
|
Re: 10 PE ISOLATES [Re: PussyFart]
#18219392 - 05/05/13 07:39 PM (10 years, 10 months ago) |
|
|
see you at the finish line hacker, nice pics
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Violet
Registered: 12/06/11
Posts: 4,205
|
Re: 10 PE ISOLATES [Re: cronicr] 1
#18219401 - 05/05/13 07:41 PM (10 years, 10 months ago) |
|
|
Hey this is awesome!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
roxxor
Lurker
Registered: 08/29/12
Posts: 251
Last seen: 8 months, 27 days
|
Re: 10 PE ISOLATES [Re: PussyFart]
#18219410 - 05/05/13 07:43 PM (10 years, 10 months ago) |
|
|
|
RiparianZoneJunky
hunter/gatherer
Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 7 months
|
Re: 10 PE ISOLATES [Re: Violet]
#18219421 - 05/05/13 07:45 PM (10 years, 10 months ago) |
|
|
Nice work man, can't wait to see the end results. There can be only one!
|
iOmniphobia
Not all who wander are lost
Registered: 03/29/13
Posts: 148
Last seen: 1 year, 24 days
|
Re: 10 PE ISOLATES [Re: roxxor]
#18219440 - 05/05/13 07:48 PM (10 years, 10 months ago) |
|
|
Well done sir. Really interesting to watch 10 isolates grow so differently. At once. Be watching this one
|
PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 months, 5 days
|
|
Thanks guys, I try.
-------------------- THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
shroom-a-zoom
Stranger in a strange land
Registered: 02/26/13
Posts: 414
Loc: Fungiland
|
Re: 10 PE ISOLATES [Re: PussyFart]
#18222292 - 05/06/13 12:53 PM (10 years, 10 months ago) |
|
|
Awesome work hacker.
-------------------- How it should & shouldn't look - NEW CULTIVATORS GUIDE Lets get rid of the SWIM shit, This also goes for my dog, someones pet ferret, and any other way of saying you are not really you. Its bullshit and we all know it. Man up and admit you are the SWIM.
|
FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
|
|
I fruited similar isolates like this a while ago..24 different plates and only one made no balls on the first flush. Ah, the elusive "clean penis" if you will
|
PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 months, 5 days
|
|
I can only hope Frank, really, this is what I am hoping for with 2, 5 and 8, as they look like they are about to explode.
-------------------- THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
PrinceShroom
Experienced Mofo
Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
Re: 10 PE ISOLATES [Re: PussyFart]
#18222399 - 05/06/13 01:20 PM (10 years, 10 months ago) |
|
|
Like Frank said no matter what you always get mutant blobs first flush. Awesome work I look forward to seeing this progress.
-------------------- WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
Sillyputty67
Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 9 years, 6 months
|
|
Post deleted by malicomReason for deletion: 1
-------------------- 1) Everything I ever posted or say is a lie.
|
Bangkok
Registered: 04/14/13
Posts: 92
Loc: Ball deep
Last seen: 3 years, 2 months
|
|
Nice work, interested in your results
|
PrinceShroom
Experienced Mofo
Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 9 years, 10 months
|
|
Quote:
malicom said: This keeps me apprehensive about growing PE. I like my non blob aa+ first flush.
The blobs are awesome though. They will have you
-------------------- WAR IS PEACE FREEDOM IS SLAVERY IGNORANCE IS STRENGTH Need help? Feel free to me.
|
Moosehead905
Stranger
Registered: 05/30/12
Posts: 244
Last seen: 6 years, 8 months
|
|
you make it look so easy.
|
Violet
Registered: 12/06/11
Posts: 4,205
|
|
My PE isolates don't blob at all… beautiful specimens for first and every flush. I didn't get any that did
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 2 months, 5 days
|
Re: 10 PE ISOLATES [Re: Violet]
#18223751 - 05/06/13 06:10 PM (10 years, 10 months ago) |
|
|
Quote:
Violet said: My PE isolates don't blob at all… beautiful specimens for first and every flush. I didn't get any that did
What was the substrate?
-------------------- THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
Violet
Registered: 12/06/11
Posts: 4,205
|
Re: 10 PE ISOLATES [Re: PussyFart]
#18223929 - 05/06/13 06:47 PM (10 years, 10 months ago) |
|
|
These are the only decent photos I have taken, tho there have been many lovely specimens on others since as well:
I bet you can guess the substrate ;] (strait grass seed)
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
Edited by Violet (05/09/13 09:38 AM)
|
TheCyndicate
Conglomerate
Registered: 10/16/11
Posts: 1,195
Loc: Outer Haven
Last seen: 5 years, 10 months
|
Re: 10 PE ISOLATES [Re: Violet]
#18224037 - 05/06/13 07:14 PM (10 years, 10 months ago) |
|
|
Nice work hacker!!! Tub 10 looks good. Cant wait to see how the others are gonna do.
Cyn
|
SLAP
Moon Shadow
Registered: 04/26/11
Posts: 62
Last seen: 10 years, 8 months
|
Re: 10 PE ISOLATES [Re: Violet]
#18224045 - 05/06/13 07:15 PM (10 years, 10 months ago) |
|
|
Awesome post!
|
|