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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis 1
#18055207 - 04/03/13 10:20 PM (10 years, 11 months ago) |
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Quite excited to be branching out and trying my first sclerotia grow!
March 20th
I prepared 3 mycobags and 2 qrt jars of rye grain (each bag contained 3.5L of hydrated grain). The grain was soaked overnight with 2% by weight/volume of gypsum. The soak water also contained coffee. I did this by adding 150g of spent coffee to the soak water (3% wt./vol of the dry volume of the grain being soaked), boiling it for 10 minutes and then decanting off the water onto the grain.
This may actually be significantly too much coffee. I didn't have access to a means of preparing fresh coffee, but what I did have was about 10kg of spent coffee grounds. The mass of coffee I used was a rough estimate based on the volume of soak water and the average mass of coffee used (per unit volume of water) to prepare a standard coffee. I then doubled the calculated mass based on the fact that I was actually using pre-used coffee. This was a very rough guess at how much I should use.
The following morning the grain was brought to the boil for 5 minutes, drained and then spread out to air dry for 30 minutes to remove all surface moisture.
The bags and jars were PC'd at 15 PSI for 3 hours.
March 21st
The bags and jars were inoculated in front of my flow box.
I did this by filling them with a plume of sterile air and adding 5mL of P. Galindoi LC. The bags were then impulse sealed and shaken up to evenly distribute the dryer grains.
The jars were shaken and then inoc'd with roughly 5mL of LC.
March 24th
Another 4 mycobags were prepared in the same manner as before. The same coffee content was once again used.
March 25th
Yesterday's mycobags were inoculated in the same manner as the first set.
At this point I was concerned at the low level of colonisation that the first set of bags were showing (4 days after being inoc'd with LC). I decided that I would inoc these bags with a further 5mL of LC. This time, the bags would not be shaken after inoculation, allowing for a more concentrated delivery of mycelium.
I performed the second inoculation by sanitising an area on the bag, placing a piece of micropore tap on this area, and inoculating through the tape. The LC was focused on the edges of the bag, so as to drain down the side. The second set of bags were also inoculated a second time in this manner.
The reason I chose to do this, is because I wanted the mycelium growth to be as uniform throughout the bags from as early as possible in their colonisation. I have read from several sources, that the mycelium of stone producing strains is not nearly as robust as that of other cubes, and that breaking up the mycelium in order to disperse it more evenly (as you do when you shake jars/bags), has been known to prevent or hinder the production of stones.
The two jars were shaken today. If not, it appeared that at the current rate of colonisation they would take a very long time to completely colonise. Also, this way I could observe the effect on the jars before deciding whether or not to shake the bags.
March 30th
Shaking the jars definitely helped to improve the rate of colonisation, and had no obvious negative influence. The mycelium does appear much finer and weaker than I observe in my shroom grows, however I think this is to be expected.
I decided to shake and re-distribute each of the bags. Upon breaking up the material I was suprised at both how much colonised material there was, and at how easily the colonised portions broke apart. The mycelium appeared quite wispy and weak.
April 2nd
A third set of 4 mycobags were prepared. This time a 2% wt./vol. of spent coffee (per unit of volume of dry grain) was used in the soak water (i.e. 120g of spent coffee for 6L of dry grain). The difference this time is that the coffee was left in the soak water, rather than being removed before adding the grain. Doing this was actually a mistake on my part. I expect that leaving the coffee in will cause problems in two possible ways; either their will be simply too much coffee (which is known to retard colonisation and stone production), and/or I will experience a significantly higher likelihood of contamination (since moulds love coffee).
Besides the coffee, no other aspect of the preparation procedure was altered.
The two quart jars were examined and seem to be progressing relatively well. It appears that the majority of the mycelium is very light and wispy, with sporadic stronger spots dispersed throughout. Will all the mycelium eventually look like this, or are these stronger points the beginnings of stone formation??.... The jars were inoc'd 12 days ago.
April 3rd
The third set of mycobags were inoculated using the same two step inoculation as used on all the previous bags (i.e. 5mL of LC, twice. Once before and once after mixing the grain).
April 4th
The first two sets of grain (7 mycobags and 2 quart jars in total), were closely examined today.
One of the mycobags had been almost consumed by trichoderma (obviously there was a small contaminated region that had been spread throughout the bag upon mixing several days ago), and was discarded. A second mycobag may also have a tiny spot of trich contamination (each of the two were inoc'd on different days). It was isolated and will be discarded in the coming days if necessary. This is a significantly higher rate of contamination that I experience with my shroom mycobags - perhaps the presence of the coffee has caused this (or simply a coincidence).
The two quart jars appear to be progressing in the same manner. Quite a bit of myc piss is now visible. They were inoc'd 14 days ago. The myc-piss stained large white growth in the upper centre of this jar is an example of the type of growth that I assume (hope!) will become a stone in the coming weeks. Can anyone with more experience comment on this?...
The first set of 3 mycobags are now almost fully colonised. They appear slightly different to the jars, as there is less variance in the appearance of all the mycelium - it appears generally thicker and stronger than the jars. Maybe this was influenced by shaking the bags after a few more days of growth than the jars? There are still however clumps of denser mycelial growth.
There is a LOT of myc piss in these bags. It isn't very noticeable in the photos, because after I had handled them it was more or less dispersed around the surface.
I'm hoping that within another week I will be confident that stones are in fact forming, at which point I'll put them aside and forget about them for a couple of months.
NOTE: I have prepared an agar culture of P. Tampanensis. Tomorrow I will prepare an LC from this. Next week I plan to prepare a further 16 quart jars and 4 mycobags (the majority of which will be P. Tampanensis).
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18074685 - 04/07/13 10:47 PM (10 years, 11 months ago) |
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April 5th
LC prepared using a 4% solution of malt extract and distilled water. The vessel was autoclaved for an hour and after cooling was inoculated with a very small (approx. 3 square mm surface area) agar wedge of P. Tampanensis.
April 6th
Prepared 8 jars and 1 mycobag (which filled my 23qt presto PC). The rye grain was washed as usual and the soak water first prepared by boiling 2% by vol/wt. spent coffee (approx. 65g), then decanting the solution. Gypsum was added, 2% wt./vol., and the grain was left to soak overnight. The next morning the grain was brought to the boil for 5 minutes and then spread out to dry for 20 minutes (as per usual procedure).
The jars and bag were PC'd for 1.5 hours at 15 PSI (I would usually PC for at least 2 hours, but I was busy and had to leave).
I won't get a chance to inoculate this substrate tomorrow, so it will have to wait until Monday.
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18074696 - 04/07/13 10:49 PM (10 years, 11 months ago) |
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nice! love the work
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: cronicr]
#18074757 - 04/07/13 10:58 PM (10 years, 11 months ago) |
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Quote:
cronicr said: nice! love the work
thanks
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18075291 - 04/08/13 03:03 AM (10 years, 11 months ago) |
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April 8th
Today I inoculated the 8 jars and 1 mycobag prepared on the 6th.
7 of the jars and the mycobag were inoc'd with the ATL#7 LC used for all previous substrate. The jars were inoc'd with approx. 4mL and the bag was inoc'd with 10mL (5mL before mixing and sealing the bag and a further 5mL after).
The last jar was inoc'd with a MS syringe of P. Galindoi that I prepared from a print I received recently. I have been unable to get this print to germinate on agar, either by swabbing the spores directly to the plate or by using MS solution from a syringe. I used about 5mL of the syringe solution and am hoping the jar will colonise so that I can use it to collect a culture sample for further work with this variety.
I checked on my previous 3 sets of mycobags today (all of which were inoc'd with the ATL#7 LC).
The three oldest bags are coming along nicely. The progress of the mycelium colonisation is noticeably different to the cubensis species. The grain appears to be colonised by a very fine mycelium which spreads throughout the medium without fusing the grain together much at all. Once this occurs, a second wave of mycelial growth seems to pass through, this time which is quite stronger and whiter in appearance and much more strongly binds the grain together (i.e. much more like the Cubensis mycelial growth I am accustomed to). Another noticeable aspect of these bags is the HUGE amount of myc piss which is being produced and pooling on the sides and at the bottom of the bags. Is this to be expected, or perhaps a result of too much coffee being added?...
As for the four bags prepared last week (those where the coffee was left in the soak water and therefore remained with the final substrate), two of them were contaminated and were disposed of. The first of these had a large growth of cobweb mould (a contaminant I've only come across once before several years ago...), and the other an already yellow/green growth of trich.
I'm convinced that the high contamination rate I've experienced (3 bags lost so far out of 11) is due to the presence of the coffee (particularly for the past 4 bags in which the spent coffee was left to remain in the bags). I prepared these bags in the same manner as I do with my Cubensis bags, where contamination is not an issue for me whatsoever.
In the future I will ensure that I never leave the spent coffee in the substrate.
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
Edited by soma_seeker (04/11/13 06:41 AM)
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18090834 - 04/11/13 06:53 AM (10 years, 11 months ago) |
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April 11th
Today I prepared 7 jars and 2 mycobags (enough to fill my PC). I prepared these in the same manner as the last set (once again removing the 2% vol/wt. coffee from the soak water before adding it to the grain).
The LC I prepared on the 5th (P. Tampanensis) contaminated. This was disappointing, as the LC I prepared using my ATL#7 culture did not cause me any problems.
I have decided that I will inoculate todays jars and bags by preparing an 'LC slurry syringe' using my first multispore agar plate of P. Tampanensis (which I have now removed sectors of for further plates and slant tubes).
This is the plate.
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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first time expert
practice makes perfect
Registered: 02/19/13
Posts: 1,246
Loc: top of mount washington
Last seen: 6 years, 6 months
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18090882 - 04/11/13 07:22 AM (10 years, 11 months ago) |
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ah Im jelious, I want some fat stones
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: first time expert]
#18090889 - 04/11/13 07:25 AM (10 years, 11 months ago) |
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Quote:
first time expert said: ah Im jelious, I want some fat stones
me too! I've never actually grown sclerotia before so I'm in the dark as to whether this will succeed and if so what I will yield
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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first time expert
practice makes perfect
Registered: 02/19/13
Posts: 1,246
Loc: top of mount washington
Last seen: 6 years, 6 months
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18090897 - 04/11/13 07:31 AM (10 years, 11 months ago) |
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Ive just heard the longer you wait the bigger they get. I saw some pics of someone who had forgot about his stones, he had to break the jars to get em out in one peice. good luck, nice set up too!!
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: first time expert]
#18096845 - 04/12/13 11:37 AM (10 years, 11 months ago) |
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Cheers
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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Farmerwhite
Cultivation student
Registered: 02/03/13
Posts: 161
Last seen: 4 years, 11 months
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18165324 - 04/25/13 08:46 AM (10 years, 10 months ago) |
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are you planning on fruiting any of these?
-------------------- I'm here to learn from the best Objects only have meaning that you put upon them
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: Farmerwhite]
#18178529 - 04/27/13 09:10 PM (10 years, 10 months ago) |
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Quote:
Farmerwhite said: are you planning on fruiting any of these?
I wasn't planning to no. Maybe in the future.
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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mycomattie
Registered: 11/15/12
Posts: 1,323
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18178652 - 04/27/13 09:37 PM (10 years, 10 months ago) |
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Awesome - I've been dabbling in P. tampanensis and they are no more difficult than cubensis (in my limited experience). I'm definitely interested in this thread - best of luck!
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soma_seeker
Stranger in aStrange Land
Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 5 months, 8 days
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: mycomattie]
#18179402 - 04/28/13 12:44 AM (10 years, 10 months ago) |
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Quote:
mycomattie said: Awesome - I've been dabbling in P. tampanensis and they are no more difficult than cubensis (in my limited experience). I'm definitely interested in this thread - best of luck!
hmm... maybe I'll have to reconsider fruiting some then
Cheers for the support.
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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IGholowan88
assassin droid
Registered: 09/03/10
Posts: 253
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#18330616 - 05/27/13 09:53 PM (10 years, 9 months ago) |
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whats an average wait like until you harvest some sclerotia? I see you inoculated on March 21st; when do you plan on cracking one of your original bags open?
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mycomattie
Registered: 11/15/12
Posts: 1,323
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: IGholowan88]
#18332330 - 05/28/13 08:14 AM (10 years, 9 months ago) |
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Quote:
IGholowan88 said: whats an average wait like until you harvest some sclerotia? I see you inoculated on March 21st; when do you plan on cracking one of your original bags open?
My understanding - at least 90 days from full colonization; but the longer you leave them, the better.
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Pass the Myce
Monkey
Registered: 11/21/14
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Re: Grow log: My first sclerotia grow. Qrt jar and mycobags. P. Galindoi & Tampanensis [Re: soma_seeker]
#20872807 - 11/21/14 10:32 PM (9 years, 3 months ago) |
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Hey dude any results or pics?
Really interesting read I'm guessing/hoping you ended with a shit load of sclerotia.
PTM
-------------------- "If we could read each other's mind's, we would all converge into one organism. What then?" "Remember the love bit" - Christopher hitchens "The beauty you see in me is a only reflection of you" - Rumi
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