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OfflineBusst
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Weed baggie prints
    #17494987 - 01/04/13 02:32 AM (1 year, 7 months ago)

Could one theoretically line a pyrex dish with a bunch of 1cm by 1cm rectangles of foil, stick them in an oven at 300.....

And then lay caps on them, letting them cover all of the foil squares, and in a SAB, use sterile tweezers, and gently put them inside of a previously unopened weed baggies that are 1 inch by 1 inch?

To use, just squirt in some water from a sterile syringe, and suck it back up?

I know it's been done this way before, but it seemed on a larger scale, one cap per print. With this way, I'm thinking we can get one cap to make about 8-10 little weed baggie prints per cap


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InvisibleBeefy1
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Re: Weed baggie prints [Re: Busst]
    #17495058 - 01/04/13 03:05 AM (1 year, 7 months ago)

Make all your prints on one big piece of clean foil in a still air box.

Cut between the prints with a razor blade.

Much easier than dealing with a bunch of tiny squares of foil.


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Re: Weed baggie prints [Re: Beefy1]
    #17495075 - 01/04/13 03:14 AM (1 year, 7 months ago)

the heat of the oven does not sound like a good idea


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Re: Weed baggie prints [Re: travelleler]
    #17495080 - 01/04/13 03:16 AM (1 year, 7 months ago)

Quote:

travelleler said:
the heat of the oven does not sound like a good idea



Agreed.


Foil from the inside of a fresh roll is about as sterile as you can get.


Dry heat from an oven will not help.  Needs to get red hot if it's dry heat.


Skip it.


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InvisibleKizzle
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Re: Weed baggie prints [Re: Beefy1]
    #17495099 - 01/04/13 03:21 AM (1 year, 7 months ago)

Don't put the whole caps into the baggies, they're covered with contaminants. Make the prints and then transfer the spores into the water.


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OfflineCodymonkeyman
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Re: Weed baggie prints [Re: Kizzle]
    #17495121 - 01/04/13 03:30 AM (1 year, 7 months ago)

Make some LC


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OfflinePussyFartM
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Re: Weed baggie prints [Re: Codymonkeyman]
    #17495125 - 01/04/13 03:31 AM (1 year, 7 months ago)

Quote:

Codymonkeyman said:
Make some LC



:smbfacepalm:


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OfflineBusst
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Re: Weed baggie prints [Re: Beefy1]
    #17495167 - 01/04/13 04:04 AM (1 year, 7 months ago)

Quote:

Beefy1 said:
Make all your prints on one big piece of clean foil in a still air box.

Cut between the prints with a razor blade.

Much easier than dealing with a bunch of tiny squares of foil.





I don't know, in my head its easier to work with the little pieces of foil...... cutting a big piece of foil w/ prints already on it seems like an easy way to dirty it up....
if they're already cut up, the caps drop on them covering it completely, and then we just take one and stick it in a bag, the spore covered foil, that is...

I am NOT suggesting we put the whole cap in the bag. Rather we take the cap, to deposit a print that covers about 9 little squares of foil, which we then put inside 9 separate baggies, each with enough spores to make a viable syringe

About the dry heat- you don't think 300 degrees in an oven will sterilize aluminum foil?


Edited by Busst (01/04/13 04:21 AM)


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OfflinePussyFartM
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Re: Weed baggie prints [Re: Busst]
    #17495180 - 01/04/13 04:16 AM (1 year, 7 months ago)

Quote:

Busst said:
cutting a big piece of foil w/ prints already on it seems like an easy way to dirty it up....



Spore prints/syringes are never 100% clean, the cap was fruited in the open air.  :shrug:



Quote:

Busst said:
I am NOT suggesting we put the whole cap in the bag. Rather we take the cap, to deposit a print that covers about 9 little squares of foil, which we then put Inside 9 separate baggies, each with enough spores to make a viable syringe



Each one would be able to make a few viable syringes.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
  ^*~.,,.~*^*~.,_,.~*^*~.,_SHIT FOR NOOBS_,.~*^*~.,_,.~*^*~.,_,.~* 


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OfflineBusst
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Re: Weed baggie prints [Re: PussyFart]
    #17495198 - 01/04/13 04:30 AM (1 year, 7 months ago)

Yeah we aren't fruiting in a sterile condition, but I figure less handling after the spores have dropped would be better than more


Edited by Busst (01/04/13 04:56 AM)


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InvisibleBeefy1
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Re: Weed baggie prints [Re: Busst]
    #17495347 - 01/04/13 06:15 AM (1 year, 7 months ago)

Quote:

About the dry heat- you don't think 300 degrees in an oven will sterilize aluminum foil?




320F for 2 hours will get it pretty close to sterile.


But it's already as close to sterile as it's going to get fresh off the roll.


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OfflinePussyFartM
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Re: Weed baggie prints [Re: Beefy1]
    #17495373 - 01/04/13 06:34 AM (1 year, 7 months ago)

Quote:

Beefy1 said:
Quote:

About the dry heat- you don't think 300 degrees in an oven will sterilize aluminum foil?




320F for 2 hours will get it pretty close to sterile.


But it's already as close to sterile as it's going to get fresh off the roll.



This whole thing is silly, it is sterile out of the factory.

You guys do realize that as soon as you take the foil out of the oven and it hits open air it is no longer "sterile" right? Thus making the entire oven thing pointless.

Prints are never 100% clean, it's a fact. This is why we use as little spores as possible when making syringes, so we have less of a chance of grabbing a contam along with the spores. This is one of the reasons why lighter syringes are better than darker ones.

Spores are microscopic and go a long way. If it's enough spores to visually see, then it's enough spores to make a syringe.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
  ^*~.,,.~*^*~.,_,.~*^*~.,_SHIT FOR NOOBS_,.~*^*~.,_,.~*^*~.,_,.~* 


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InvisibleFungal growth
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Re: Weed baggie prints [Re: PussyFart]
    #17495664 - 01/04/13 09:57 AM (1 year, 7 months ago)

Quote:

Notahacker420 said:
Quote:

Beefy1 said:
Quote:

About the dry heat- you don't think 300 degrees in an oven will sterilize aluminum foil?




320F for 2 hours will get it pretty close to sterile.


But it's already as close to sterile as it's going to get fresh off the roll.



This whole thing is silly, it is sterile out of the factory.

You guys do realize that as soon as you take the foil out of the oven and it hits open air it is no longer "sterile" right? Thus making the entire oven thing pointless.

Prints are never 100% clean, it's a fact. This is why we use as little spores as possible when making syringes, so we have less of a chance of grabbing a contam along with the spores. This is one of the reasons why lighter syringes are better than darker ones.

Spores are microscopic and go a long way. If it's enough spores to visually see, then it's enough spores to make a syringe.



:awesomenod:
KISS. baggies, foil, saran wrap, etc. are sold sterile. there are no degrees of sterile. it is or isn't.


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OfflineBusst
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Re: Weed baggie prints [Re: Fungal growth]
    #17495707 - 01/04/13 10:23 AM (1 year, 7 months ago)

Sure, maybe its sterile out of the factory. But once your fingers touch it, unroll it, cut it, whatever, its not sterile anymore  sure maybe prints themselves aren't sterile, but if you can avoid handling a print, it will have a higher chance of success. I won't believe otherwise. The smaller the window for an outside spore to settle on the foil, the better


Edited by Busst (01/04/13 10:25 AM)


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InvisibleFungal growth
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Re: Weed baggie prints [Re: Busst]
    #17495789 - 01/04/13 10:47 AM (1 year, 7 months ago)

k. imo, it's a waste of time. but it's your time, not mine.
but if your gonna, it would be better to use jars with the foil confetti on bottom, so they can be pc'd. fuck that oven.


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Offlinetwistedty
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Re: Weed baggie prints [Re: PussyFart]
    #17496010 - 01/04/13 11:57 AM (1 year, 7 months ago)

Quote:

Notahacker420 said:
Quote:

Codymonkeyman said:
Make some LC



:smbfacepalm:





:rofl:

i hate LCs!


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Invisibleserotonin storm
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Re: Weed baggie prints [Re: twistedty]
    #17496455 - 01/04/13 01:31 PM (1 year, 7 months ago)

I for one think that this could be a killer idea. I think you may be going about it the wrong way though.

If you have SAB big enough or a flow hood, you could just do it all in side there because as stated the foil is near sterile. If you don't like this the suggestion of using a jar so you can pressure cook it seems to be a better route than using the oven. I really like this idea of having perfectly proportioned foil squares ready to go, if you go about it the right way.


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Offlinehoobatech
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Re: Weed baggie prints [Re: Busst]
    #17496828 - 01/04/13 02:56 PM (1 year, 7 months ago)

Quote:

Busst said:
Could one theoretically line a pyrex dish with a bunch of 1cm by 1cm rectangles of foil, stick them in an oven at 300.....

And then lay caps on them, letting them cover all of the foil squares, and in a SAB, use sterile tweezers, and gently put them inside of a previously unopened weed baggies that are 1 inch by 1 inch?

To use, just squirt in some water from a sterile syringe, and suck it back up?

I know it's been done this way before, but it seemed on a larger scale, one cap per print. With this way, I'm thinking we can get one cap to make about 8-10 little weed baggie prints per cap





I think for ease of use try tmethyls setup

just put your cap on the lid and let it drop spores, then place it back onto the container of sterile water and shake.


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Invisible36fuckin5
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Re: Weed baggie prints [Re: hoobatech]
    #17496885 - 01/04/13 03:04 PM (1 year, 7 months ago)

Quote:

hoobatech said:
I think for ease of use try tmethyls setup

just put your cap on the lid and let it drop spores, then place it back onto the container of sterile water and shake.




That works if you just want syringes, but for long-term storage, you want prints.


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OfflineMycroProphecy
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Re: Weed baggie prints [Re: Busst]
    #17497268 - 01/04/13 04:26 PM (1 year, 7 months ago)

Last time I made prints I cut up a bunch of pieces of aluminum foil, stuck them in a quart jar, put them in the oven for 1 hour at 300 degrees. Then take out pieces as needed in SAB and continue printing/drying there as well. All on the bottom of my SAB. I haven't actually used any of them to make syringes yet so I can't say for sure on accuracy.
Quote:

Busst said:
Sure, maybe its sterile out of the factory. But once your fingers touch it, unroll it, cut it, whatever, its not sterile anymore  sure maybe prints themselves aren't sterile, but if you can avoid handling a print, it will have a higher chance of success. I won't believe otherwise. The smaller the window for an outside spore to settle on the foil, the better



Completely agreed. Always use sterile technique when printing and inoculating.


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Edited by MycroProphecy (01/04/13 04:29 PM)


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