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I have my procedure down pretty well and feel completely ready to start but I am concerned about contamination. I have made a still air box and know the basic ways to try to prevent contamination in my area but i wanna make sure that I don't have to throw away a cake So, basically I would like to know how to be contam free as possible with this. How problematic is it? Thanks
i just started as well but i had no contams in my first 9 jars by doing all my noccing up in my closet, in a SAB. i lysol'd the shit out of the closet and just outside of it, took a shower, put on clean clothes, used gloves, wiped down my entire work station and my arms and hands with 70%iso and then after noccing up, left them in the SAB and have had no problems, oh and i put a second shirt on just over my nose and mouth as a mask. the biggest thing i didnt do was shaking the syringe between every jar 2 of my jars didnt germinate at all, one of them germinated at one noc point, 2 of them only germinated at 2 noc points, and 2 only germinated at 3 noc points, the other 2 all 4 points germinated nicely. but good luck man! oh yeah and i steam sterilized for 110 mins, which may have been a bit long but it sure as hell got em sterile! edit: i also used micropore tape and nocced straight through the tape while flame sterilizing my needle between every jar using a homemade alcohol lamp. (outside of the SAB of course!)
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