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 bigd17
Registered: 07/31/08
Posts: 39
Loc: Richardson, Texas
Last seen: 2 months, 13 days
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 Proper Use of LC question 
#16726775 - 08/19/12 03:33 PM (8 months, 24 days ago) |
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Hey everyone. A FOAF is planning on using some spores to create liquid cultures to grow from. He is using cubensis spores if that makes any difference. After doing lots of research he has found much information regarding how to make and store LC but thus far has been unable to find the method by which the LC is applied to a substrate (I'm assuming via syringe) and how much is necessary for successful inoculation. My friend was thinking of using 1 gallon rectangular rubber made containers with WBS as the substrate.
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"The secret to success is to make your Vocation a Vacation."
-Mark Twain
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 Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
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Re: Proper Use of LC question [Re: bigd17]
#16726787 - 08/19/12 03:35 PM (8 months, 24 days ago) |
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LC is a huge waste of time, don't even bother with it.
Also, you're skipping some steps there.
Where you want to start is PF Tek, that's got the best chance of working well the first time.
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You make it all possible. Doesn't it feel good?
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jenkinz
Parliament of Trees Ambassador
Registered: 04/02/12
Posts: 414
Last seen: 1 month, 1 day
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Re: Proper Use of LC question [Re: Doc_T]
#16726941 - 08/19/12 04:06 PM (8 months, 24 days ago) |
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Just start with the spores to inoculate some PF jars. It'll help you learn the fundamentals and good sterile procedure. Once you're comfortable and successful you can apply what you've learned to bulk substrates. LC is an extra step that can potentially add a lot of problems if you don't do it right.
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 Notahacker420
Victory is Mine!
Registered: 04/08/12
Posts: 9,778
Loc: Quahog, Rhode Island
Last seen: 2 minutes, 31 seconds
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Re: Proper Use of LC question [Re: jenkinz]
#16726952 - 08/19/12 04:09 PM (8 months, 24 days ago) |
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Its not even about doing it right....LC from spores is a gamble.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
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GT Cluster Clone Monotubs, My Greenhouse, Up-to-date Cultivation Info, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber),
Monotub Tek, Damien5050's Coir Tek, Substrate Calculator, HippieChick's Greenhouse, TL's G2G in a SAB, TL's One Flush Wonder Tek, TL's WBS Tek,
TL's GLC TEK, TL's Alcohol Extraction TEK , TL's Still Air Box TEK, Common Contaminations, Frank's Proper Pasteurization Tek, EvilMushroom666's Pasteurization Tek
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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OP, watch these free video clips:
http://www.mushroomvideos.com/BRF-Pf-Tek
http://www.mushroomvideos.com/Terrarium-Tek
Roger Rabbit shows the PF Tek there. Do it like that, you'll like the results. 
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You make it all possible. Doesn't it feel good?
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Rahz
Dead Gone Forgotten
Registered: 11/11/05
Posts: 5,069
Last seen: 7 hours, 20 minutes
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Re: Proper Use of LC question [Re: bigd17]
#16726988 - 08/19/12 04:18 PM (8 months, 24 days ago) |
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Quote:
bigd17 said:
Hey everyone. A FOAF is planning on using some spores to create liquid cultures to grow from. He is using cubensis spores if that makes any difference. After doing lots of research he has found much information regarding how to make and store LC but thus far has been unable to find the method by which the LC is applied to a substrate (I'm assuming via syringe) and how much is necessary for successful inoculation. My friend was thinking of using 1 gallon rectangular rubber made containers with WBS as the substrate.
LC is generally applied to spawn jars (sterilized grain), ideally through an inoculation port.
Ideally the LC jar will also have an inoculation port. Read up on LC lids (there's one in my journal), and grain prep, sealant ports, etc.
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rahz
comfort pleasure power love truth awareness peace
"A fool thinks himself to be wise, but a wise man knows himself to be a fool."
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