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 mellowparty
legitimate researcher
Registered: 05/17/09
Posts: 17,486
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 I killed a mouse and had fun with its brain  8
#16622220 - 07/31/12 02:53 PM (9 months, 15 days ago) |
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I injected the animal with 20% sodium pentobarbitone in the heart, subsequently perfused it with half a liter 4% paraformaldehyde and opened its skull.
I extracted the brain and preserved it in formaldehyde at 4oC. Then I cryoprotected the brain in 30% sucrose and sliced it into thick slabs. Subsequently I embedded the brain slabs in special media and sliced it into 6 micron sections. Then I stained it with toluidine blue dye. You can see the neurons in the cortex and what appears to be the hippocampus with the various Cornu Ammonis areas.
          
I hope you enjoyed this post as much as I enjoyed playing with the brain.
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 moonrockmushy
certifiedpoopface
Registered: 07/01/05
Posts: 5,521
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 Re: I killed a mouse and had fun with its brain [Re: mellowparty] 3
#16622267 - 07/31/12 02:59 PM (9 months, 15 days ago) |
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You're a monster.
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mellowparty
legitimate researcher
Registered: 05/17/09
Posts: 17,486
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Re: I killed a mouse and had fun with its brain [Re: moonrockmushy]
#16622282 - 07/31/12 03:02 PM (9 months, 15 days ago) |
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Actually I needed another part of the mouse for a legit study approved by an ethics committee. I kept the brain cause it seemed interesting and it would've been a waste to just throw it out as I was supposed to.
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NWlight
Just look
Registered: 01/13/10
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Re: I killed a mouse and had fun with its brain [Re: mellowparty]
#16622297 - 07/31/12 03:05 PM (9 months, 15 days ago) |
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:awescience:
I quit a job because I had to do stuff on animals but I realize that it's an extremely effective method of making advances in medicine 
We were simulating the symptoms of Preeclampsia by putting clamps on the organs of live mice in a few locations... bit much for a vegetarian 
GO SCIENCE! 
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Hilarious and inconsequential, the lot of it. Enjoy the ride. Music is medicine.  
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mellowparty
legitimate researcher
Registered: 05/17/09
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Re: I killed a mouse and had fun with its brain [Re: NWlight]
#16622354 - 07/31/12 03:14 PM (9 months, 15 days ago) |
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I didn't feel bad for the mouse because it wasn't feeling any pain. I used isoflurane-halothane anaesthesia before the pentobarbitone shot.
I wish I could've injected myself with that pentobarbitone but the mouse needed it more than me
I discovered a new receptor that gets upregulated immediately after nerve injury so it was adopted by Pfizer as a new drug target for neuropathic pain treatment. The fuckers never informed me on their progress but I had to give them a detailed report  I feel angry about that.
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BothHands
Dog Coffee
 
Registered: 10/28/09
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Re: I killed a mouse and had fun with its brain [Re: mellowparty]
#16622365 - 07/31/12 03:17 PM (9 months, 15 days ago) |
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Would you like to study my brain? All I require is some pentobarbitone. 
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Put America to sleep with warm milk and clichés.
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mellowparty
legitimate researcher
Registered: 05/17/09
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Re: I killed a mouse and had fun with its brain [Re: BothHands]
#16622379 - 07/31/12 03:19 PM (9 months, 15 days ago) |
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I would love to explore other parts of your body but sure I'll give you a shot 
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Soulidarity
All Good Things Come To An End
Registered: 07/15/12
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Re: I killed a mouse and had fun with its brain [Re: mellowparty]
#16622402 - 07/31/12 03:22 PM (9 months, 15 days ago) |
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so what possessed you to fillet the brain of a mouse and inject it with dr00gz. hopefully this was some kind of research project or had some legitimate purpose to it.
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NWlight
Just look
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Re: I killed a mouse and had fun with its brain [Re: BothHands]
#16622405 - 07/31/12 03:23 PM (9 months, 15 days ago) |
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Quote:
I discovered a new receptor that gets upregulated immediately after nerve injury so it was adopted by Pfizer as a new drug target for neuropathic pain treatment
The only thing I have "discovered" was a bump on the knee that is in 75% of the population but never mentioned in text books. Might get to name it 
It doesn't affect anybody in the world besides orthopedic surgeons though
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Hilarious and inconsequential, the lot of it. Enjoy the ride. Music is medicine.
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NWlight
Just look
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Re: I killed a mouse and had fun with its brain [Re: Soulidarity] 1
#16622408 - 07/31/12 03:24 PM (9 months, 15 days ago) |
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Quote:
Soulidarity said:
so what possessed you to fillet the brain of a mouse and inject it with dr00gz. hopefully this was some kind of research project or had some legitimate purpose to it.
Summarized:
Quote:
*starts reading OP*
blah blah big words blah blah...
So..... was there a point to this thread?
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Hilarious and inconsequential, the lot of it. Enjoy the ride. Music is medicine.
Edited by NWlight (07/31/12 03:59 PM)
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ellenallien
lumpy space princess
Registered: 12/04/08
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Re: I killed a mouse and had fun with its brain *DELETED* [Re: mellowparty]
#16622434 - 07/31/12 03:29 PM (9 months, 15 days ago) |
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Post deleted by ellenallienReason for deletion: T . M . I
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BothHands
Dog Coffee
Registered: 10/28/09
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Re: I killed a mouse and had fun with its brain [Re: mellowparty]
#16622438 - 07/31/12 03:30 PM (9 months, 15 days ago) |
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Quote:
mellowparty said:
I would love to explore other parts of your body but sure I'll give you a shot
When I'm dead, explore away. Most people aren't lucky enough to die blissfully on barbiturates.
Quote:
ellenallien said:
when i lost my mind on benzo's, i live in the city. when the mexicans moved out next door, their mice moved in here.
i got the sticky glue traps and i caught 7 babys glued to one trap one time but thats off topic.
i went crazy and lost my mind for a bit, i caught a few of them on a glue trap and tortured them. i really was sick at the time
ive never done anything like that before in my life. i really was sick. i only did it a few times but i feel remorse for it now
not the catching and throwing them away, but the torture.
i see it this way. they are home invaders using chemical warfare. their feces is toxic. they will drop it in our food and drag it anywhere else
across your house and they dont care if they get you sick it can even be fatal. so i think its far game to kill them
I really wish I never heard that.
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Put America to sleep with warm milk and clichés.
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Soulidarity
All Good Things Come To An End
Registered: 07/15/12
Posts: 4,776
Loc: with the fallen angels
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Re: I killed a mouse and had fun with its brain [Re: ellenallien]
#16622454 - 07/31/12 03:32 PM (9 months, 15 days ago) |
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dude thats fucked up 
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ellenallien
lumpy space princess
Registered: 12/04/08
Posts: 8,142
Loc: Sanriotown, Harmonyland
Last seen: 16 days, 23 hours
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Re: I killed a mouse and had fun with its brain [Re: Soulidarity]
#16622483 - 07/31/12 03:36 PM (9 months, 15 days ago) |
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well.. i cant take it back. i was really mental and suicidal at the time. if i didnt care about my own life then whats a little mouse to me ?
anyway like i said i really regret it, sorry you had to hear that. i shouldnt be so open about things 
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Soulidarity
All Good Things Come To An End
Registered: 07/15/12
Posts: 4,776
Loc: with the fallen angels
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Re: I killed a mouse and had fun with its brain [Re: ellenallien]
#16622519 - 07/31/12 03:42 PM (9 months, 15 days ago) |
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everyone makes mistakes, as long as you learn from them.... then theres not that much of an issue.
but why are you more worthy of life then then those mice?
to my mind theres a big difference between someone who is indifferent to what happens to a mouse, and someone who chooses to go out of their way to torture and inflict pain on a mouse. thats like psychologically bad shit.
what if a creature of some form that was experiencing turmoil came along and saw fit to torture you or something? you obviously wouldn't be very appreciative of that either.
i dunno dude i just hate hearing shit about animals being harmed un-necessarily. any poster who posted the same post would have elicited the same response from me, so it's nothing personal.
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mellowparty
legitimate researcher
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Re: I killed a mouse and had fun with its brain [Re: NWlight]
#16622560 - 07/31/12 03:47 PM (9 months, 15 days ago) |
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Quote:
Soulidarity said:
so what possessed you to fillet the brain of a mouse and inject it with dr00gz. hopefully this was some kind of research project or had some legitimate purpose to it.
As I said it was part of a legit study on neuropathic pain. I guess I'm just curious cause I had no rational reason to cut its brain, I did because I wanted to see whats inside.
Quote:
NWlight said:
Quote:
I discovered a new receptor that gets upregulated immediately after nerve injury so it was adopted by Pfizer as a new drug target for neuropathic pain treatment
The only thing I have "discovered" was a bump on the knee that is in 75% of the population but never mentioned in text books. Might get to name it
It doesn't affect anybody in the world besides orthopedic surgeons though
I didn't discovered the receptor per se. What I discovered was that a receptor normally expressed only in the immune system was significantly upregulated after nerve injury and its expression pattern correlated with electrophysiological aberrations that relate to neuropathic pain. I was also blessed by the god of serendipity and randomly stumbled upon a very peculiar spatial pattern of expression in the ganglion (CNS glia had it PNS glia didn't; it was so beautiful down the microscope).
Quote:
NWlight said:
So..... was there a point to this thread?
To show you a mouse brain and some cells. Geez if a thread is not about drugs and/or DSHSB/buttseks then everyone goes 
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NWlight
Just look
Registered: 01/13/10
Posts: 10,647
Last seen: 20 minutes, 7 seconds
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Re: I killed a mouse and had fun with its brain [Re: mellowparty]
#16622587 - 07/31/12 03:50 PM (9 months, 15 days ago) |
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Quote:
Quote:
NWlight said:
So..... was there a point to this thread?
To show you a mouse brain and some cells. Geez if a thread is not about drugs and/or DSHSB/buttseks then everyone goes
 I was merely summarizing that guys post, specifically noting that he clearly did not read the OP
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Hilarious and inconsequential, the lot of it. Enjoy the ride. Music is medicine.
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mellowparty
legitimate researcher
Registered: 05/17/09
Posts: 17,486
Last seen: 2 months, 7 days
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Re: I killed a mouse and had fun with its brain [Re: NWlight]
#16622630 - 07/31/12 03:56 PM (9 months, 15 days ago) |
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lol my bad I'm nodding too hard
Carry on
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trekie
Registered: 05/11/09
Posts: 4,760
Loc: Large city
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Re: I killed a mouse and had fun with its brain [Re: NWlight]
#16622635 - 07/31/12 03:57 PM (9 months, 15 days ago) |
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My girl gasses them around 20-30 at a time and grinds up there organs to extract RNA out of them.
Science kicks ass 
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I must not fear. Fear is the mind-killer. Fear is the little-death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.
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mellowparty
legitimate researcher
Registered: 05/17/09
Posts: 17,486
Last seen: 2 months, 7 days
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Re: I killed a mouse and had fun with its brain [Re: trekie]
#16622655 - 07/31/12 03:59 PM (9 months, 15 days ago) |
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What kind of RNA? Thats my favourite class of macromolecules.
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