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 navyseals101
Stranger
Registered: 09/16/04
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 Tis been a while 
#16525578 - 07/13/12 03:17 PM (10 months, 2 days ago) |
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since i did a grow. I was wondering if anyone knew if agar still made is lids that had the filter disk and injection port?
Also, can these be used for liquid cultures?
Is there an easy way around this?
whats the easiest way to get a sterile liquid culture going?
Whats the best way to inoculate jars? Are filter jars a huge improvement in terms of reduction of contamination?
Are spawn bags easier to use / more efficient as far as bulk goes?
whats the best grain to use ( in the bast I used wild bird seed but i had like half my jars contaminate for some unknown reason)
Why is rye berries used? what is a rye berrY? are they hard to find? is its extra cost over wild birds seed worth it? How come?
any additional advice would be greatly appreciated. will be growing with the golden teacher variety, but I am confused as to how to get a good culture going. Will I need to try to isolate a strain?/ would that help? roger rabbit iirc used to have a guide on how to isolate the characteristics of a single spore and grow out subsequent generation and isolate certain ones, but i forget where the link went.
does anyone have any pointers here? have grown before but still have some questions
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"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
-wumen
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 Fungal growth
Lootinint
Registered: 03/21/10
Posts: 3,537
Loc: under a rock in your yard
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where's the search engine?
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navyseals101
Stranger
Registered: 09/16/04
Posts: 999
Last seen: 9 days, 19 hours
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I'm having trouble phrasing my questions, but do you guys understand what it is that i'm asking? I don't know how to phrase what i'm looking for without pulling up all kinds of irrelevant topics so i figured that i'd ask the community..
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"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
-wumen
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Queen of Kings
Get on with the Fascination
Registered: 12/11/11
Posts: 922
Loc: Pacific Northwest, almost
Last seen: 3 hours, 59 minutes
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Most people post one question looking for input.
You have posted about a billion. Seriously.
I read and read and researched and bookmarked for months here before my first grow.
It looks like you'd like the entire experience of mushroom growing laid out neatly in one place.
Roger Rabbit's videos do that pretty nicely.
Damion5050's coir tek explains bulk nicely.
There are a gazillion threads about rye, and why it's better. (It is, for me.)
There ar e many ways to make agar as well as liquid culture. Simple search terms that will have you reading for days.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
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Forget all about LC.
Agar is where you want to be. AGar work is cheaper and easier than it looks.
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You make it all possible. Doesn't it feel good?
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navyseals101
Stranger
Registered: 09/16/04
Posts: 999
Last seen: 9 days, 19 hours
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Re: Tis been a while [Re: Doc_T]
#16526014 - 07/13/12 04:46 PM (10 months, 2 days ago) |
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Doc_T can you explains?
wouldn't liquid culture be better because you can create large volumes of inocculant sp??
or is the same true with agar?
would it be easy to fill a vat with agar and colonize it and then try to extract mycelium? / psil
my questions are geared towards sterile technique and the viability of methods.
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"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
-wumen
Edited by navyseals101 (07/13/12 04:52 PM)
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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You want large amounts of spawn, try grain to grain expansion.
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You make it all possible. Doesn't it feel good?
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navyseals101
Stranger
Registered: 09/16/04
Posts: 999
Last seen: 9 days, 19 hours
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Re: Tis been a while [Re: Doc_T]
#16526077 - 07/13/12 04:57 PM (10 months, 2 days ago) |
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but wouldn't inocculation through a liquid culture take only 10 days whereas initial inoculation with a spore will take a month to get the jar fully colonized?
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"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
-wumen
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Did you come here for advice or an argument?
Do it any way you like. Don't blame me if it fails. 
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You make it all possible. Doesn't it feel good?
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Mateo
High on LIFE!
Registered: 06/24/11
Posts: 470
Loc: North Europe
Last seen: 6 months, 9 days
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Re: Tis been a while [Re: Doc_T]
#16526424 - 07/13/12 06:00 PM (10 months, 2 days ago) |
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I have some advice for you.
Don't start with LC, if half of your jars contaminated by some unknown reason then LC's is out of the question.
Why?
LC's are super fast for cononization of substrates but they are also super sesitive to contaminations.
There are other ways.
Do ryegrain jars or bags, not ryeberries, what you want is rye.
The whole grain of rye, sometimes stupid people call these ryeberries.
Either you take the time to look into agar work and inoculate your ryegrain jars/bags with an colonized agar piece or you inoculate your rye jars/bags with a sporesyringe.
I suggest you look into agar work but can do some sporesyringes right now.
Rye is probably the best grain to use, thats why the growers who do this for a living use rye.
Although it can depend on what mushroom you want to grow ofcause.
But rye can handle the most.
When you have got a small jar of ryegrain colonized with your mycelium and you see that it is clean you can do a GLC (grain liquid cilture).
This is basicly an LC but as you do it from you clean grains you can be certain its clean witch isn't the case with ordinary LC's.
Just look up a tek on this site.
I prefer to do G2G, grain to grain.
Just take one of your colonized grainjars and pour some into a batch of new sterilized grainjars.
Shake and it colonizes very fast.
You must do some reading up on these things either online or by reading P.Stamets 2 good books on mushroom cultivation.
They are "The Mushroom Cultivator" and "Growing Gourmet and medicinal mushrooms".
These 2 books explains ALL things in detail and can be used as a reference when you get into trouble or just dont know what todo next.
Super recomended.
Now i have bla bla too much.
It's the vodka maybee...?
Good luck though..
/Mateo
Have you seen RR's mushrom cultivation videos?
I think thats where you need to start, they are very good.
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"I am That, You are That, We are That and That's That."
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Queen of Kings
Get on with the Fascination
Registered: 12/11/11
Posts: 922
Loc: Pacific Northwest, almost
Last seen: 3 hours, 59 minutes
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Re: Tis been a while [Re: Mateo]
#16527598 - 07/13/12 10:24 PM (10 months, 2 days ago) |
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I like liquid culture and agar. Why not?
Liquid culture gets a bad name because you can't tell it's got a contamination until you try and grow it, thus potentially ruining jars.
Agar, on the other hand, tips you off right away.
It's good not to put all your eggs in the same basket with mushrooms. Diversify.
Do you have a pressure cooker?
When I get a new syringe, I (a) inoculate BRF cakes, (b) make a small half-pint jar of liquid culture, and (b) put a few drops into a petri dish (or two).
Multiple coverage!! You would have a hard time failing in all three endeavors (though I don't doubt it can be done). 
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