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 OICU812
Chefs Choice Mushroom Co.
 
Registered: 11/06/11
Posts: 751
Loc: Foothills of NC 
Last seen: 3 hours, 57 minutes
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 Re: Things every lab/clean room should have  [Re: Terry M]
#16510878 - 07/10/12 08:07 PM (11 months, 2 days ago) |
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Quote:
Terry M said:
I always have a list of things to do printed out for that day's lab session. It allows me to line up my plates, grain jars, etc. into sequential order for simple work flow. I check off a task when I have done it. Since I do lots of different species, this list can get long and varied.
But I've found that if I try to do too much in one session, I often screw something up towards the end. Usually it's not serious, and just results in a wasted grain jar or something. Prolonged lab work can be tiring, especially as the lab heats up from the flow hood and turned off AC. But it's hard to resist putting too much on the list when me and my work area are sterile and ready to go!
An excellent idea, Terry. I tend to be an over achiever too and it has consequences from time to time!
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"Human beings, who are almost unique in having the ability to learn from the experiences of others,
are also remarkable for their apparent disinclination to do so." - Douglas Adams
“Experience is the best teacher . . . and fools will
learn from no other" - my Grandmother
Forget injuries, never forget kindnesses. - Confucius
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chefinainteasy
Chef
Registered: 12/13/10
Posts: 887
Last seen: 2 months, 6 days
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Re: Things every lab/clean room should have [Re: OICU812]
#16511011 - 07/10/12 08:40 PM (11 months, 2 days ago) |
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Slightly off topic but as good a place as any, those of you who do lots of grain prep at home or small scale, in what and how do you cook your grains. Basically what size batches do you do? At work I have access to large pots, big steam jacket kettles, and a 12 burner range. So I usually do 25# dry rye batches. But I also have large colanders and basically everything else. Just wondering if you guys have a good method for bulk prep?
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Amanita virosa
botanist by day
Registered: 12/04/11
Posts: 1,556
Loc: north kakalacky
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Re: Things every lab/clean room should have [Re: Terry M]
#16511127 - 07/10/12 09:15 PM (11 months, 2 days ago) |
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Quote:
Terry M said:
And when you're spraying bleach solution all around a small, closed room, you will definitely need a 3M 7502 respirator or equivalent. This needs a pair of 6006 cartridges which filter out the bleach so that you can't even smell it from inside the respirator.
I also always wonder if the 10% bleach does a number on my hepa filter?? whatcha think?
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Terry M
Stranger in a Strange Land
Registered: 06/18/10
Posts: 1,375
Loc: Rhode Island
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Re: Things every lab/clean room should have [Re: Amanita virosa]
#16511470 - 07/10/12 10:27 PM (11 months, 2 days ago) |
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Quote:
Amanita virosa said:
I also always wonder if the 10% bleach does a number on my hepa filter?? whatcha think?
I don't know the answer. But before I fill the air with bleach (+ vinegar), I cover my entire work table with a heavy plastic tarp. This table has my flow hood, microscope, and a laptop for the microscope camera.
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 Aleon
Void, Form, and Formless
Registered: 05/26/11
Posts: 444
Loc: WI, USA
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Re: Things every lab/clean room should have [Re: Terry M]
#16512166 - 07/11/12 12:34 AM (11 months, 2 days ago) |
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Quote:
Terry M said:
and a laptop for the microscope camera.
 Im jealous! Thats sounds like lots of fun; i might never leave my lab! 
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"And the day came when the risk to remain tight in a bud was more painful than the risk it took to blossom."
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Javadog
Continuing along
Registered: 05/03/10
Posts: 7,326
Loc: USA
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Re: Things every lab/clean room should have [Re: Aleon]
#16512237 - 07/11/12 12:51 AM (11 months, 2 days ago) |
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Yup...Terry is a gear-head, a very good one too.
JD
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Terry M
Stranger in a Strange Land
Registered: 06/18/10
Posts: 1,375
Loc: Rhode Island
Last seen: 1 day, 7 hours
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Re: Things every lab/clean room should have [Re: Javadog]
#16513141 - 07/11/12 08:41 AM (11 months, 1 day ago) |
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I use the camera only occasionally, to help find my way in real time when I get lost on a microscope slide. My cell phone takes better pictures! And the laptop is a retired one that was replaced from regular use because the LCD display has a line through it. But it's perfect for infrequent duty.
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solarity
mm... my favourite food
Registered: 03/31/09
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Re: Things every lab/clean room should have [Re: Amanita virosa]
#16513973 - 07/11/12 01:38 PM (11 months, 1 day ago) |
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Reply Moved to new "on topic" thread here to avoid highjack!
Edited by solarity (07/11/12 05:41 PM)
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chefinainteasy
Chef
Registered: 12/13/10
Posts: 887
Last seen: 2 months, 6 days
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Re: Things every lab/clean room should have [Re: solarity]
#16514358 - 07/11/12 03:20 PM (11 months, 1 day ago) |
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Really, PC for 3.5 hours. I only do 2 hours at 15 psi. I usually put the gypsum in the water when im boiling it. Do you notice any benefit from adding it after it is drained besides there is none wasted in the water?
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chefinainteasy
Chef
Registered: 12/13/10
Posts: 887
Last seen: 2 months, 6 days
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Re: Things every lab/clean room should have [Re: chefinainteasy]
#16522538 - 07/12/12 11:27 PM (11 months, 3 hours ago) |
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So how far is the reach, that is distance from the front of the filter, of laminar flow? How big should my work surface be in front of the flow hood?
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Amanita virosa
botanist by day
Registered: 12/04/11
Posts: 1,556
Loc: north kakalacky
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Re: Things every lab/clean room should have [Re: chefinainteasy]
#16529270 - 07/14/12 09:04 AM (10 months, 29 days ago) |
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So I am also planning a new lab space; this time, i have some financial backing and am wondering about how to cool the lab portion of the building. the rest of the space is climate controlled. I was thinking I would suck air thru a hepa from the outside room, also creating some positive pressure in the lab. Question: is there an advantage to doing this in the ceiling versus a side wall? also, will the motor that is sucking the air heat it up to the point that it wont really be cool any more. mostly concerned with cooling, not heating of the space. Right now i am in a space that is 8x10 with a four foot series three hood and it is COOKING MY BRAIN!!
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OICU812
Chefs Choice Mushroom Co.
Registered: 11/06/11
Posts: 751
Loc: Foothills of NC
Last seen: 3 hours, 57 minutes
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Re: Things every lab/clean room should have [Re: Amanita virosa]
#16531801 - 07/14/12 09:51 PM (10 months, 29 days ago) |
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Quote:
Amanita virosa said:
...I was thinking I would suck air thru a hepa from the outside room, also creating some positive pressure in the lab.
Your lab air should be pushed through the HEPA as it enters the lab. Using the HEPA the way you would use a regular HVAC filter is wrong. All HVAC systems leak air. Non-filtered, contaminated air would be mixed with your clean air before reaching the lab.
Quote:
Question: is there an advantage to doing this in the ceiling versus a side wall?
I have seen HEPAs mounted both ways. Mine is mounted directly over the lab bench to allow cool air to fall directly on the lab technician.
Quote:
also, will the motor that is sucking the air heat it up to the point that it wont really be cool any more.
Not if it is properly sized for the load it will be under.
Edited by OICU812 (09/13/12 10:55 PM)
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Amanita virosa
botanist by day
Registered: 12/04/11
Posts: 1,556
Loc: north kakalacky
Last seen: 9 hours, 21 minutes
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Re: Things every lab/clean room should have [Re: OICU812]
#16533698 - 07/15/12 09:16 AM (10 months, 28 days ago) |
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right! sucking was a poor choice of words. the blower would be outside the lab pushing air thru the filter. I am following this thread closely and have learned alot! Thanks.
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a social revolt
retired cultivator
Registered: 09/07/11
Posts: 833
Loc: Brooklyn, NYC
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Re: Things every lab/clean room should have [Re: chefinainteasy]
#16535288 - 07/15/12 04:53 PM (10 months, 28 days ago) |
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This is such a great thread
I too am in the midst of setting up a lab and this is so so so so so helpful!
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DRCola
Registered: 05/04/11
Posts: 111
Loc: USA
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Re: Things every lab/clean room should have [Re: OmSource]
#16837859 - 09/12/12 11:05 PM (9 months, 3 hours ago) |
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Great ideas and info in this post, and I have a discussion topic related to panda film, plastic, etc.
What are your thoughts on lining the lab with medical Tyvek®?
My idea/dream would be to cut it from lab coats and seam it with Tyvek® tape. It seems the building-type roll vapor barrier MIGHT work, but may be less of a contamination barrier.
I'm not sure if this would be a fire hazard, Dupont states not to use it around open flame.
Any thoughts are appreciated--before I get too far along with this idea.
Thanks all !
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Terry M
Stranger in a Strange Land
Registered: 06/18/10
Posts: 1,375
Loc: Rhode Island
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Re: Things every lab/clean room should have [Re: DRCola]
#16839373 - 09/13/12 06:50 AM (8 months, 30 days ago) |
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I think a Tyvek lab interior lining is a bad idea. The interior surfaces should be sealed, non-porous and easily cleanable. Contaminants enter the lab via you and the door you walk in, not through the walls.
Since Tyvek is highly porous, it would be a bitch to clean. Unless you autoclaved the whole lab, of course!
Regards,
Terry
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Amanita virosa
botanist by day
Registered: 12/04/11
Posts: 1,556
Loc: north kakalacky
Last seen: 9 hours, 21 minutes
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Re: Things every lab/clean room should have [Re: Terry M]
#16839525 - 09/13/12 08:25 AM (8 months, 30 days ago) |
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Quote:
Terry M said:
I think a Tyvek lab interior lining is a bad idea. The interior surfaces should be sealed, non-porous and easily cleanable. Contaminants enter the lab via you and the door you walk in, not through the walls.
Since Tyvek is highly porous, it would be a bitch to clean. Unless you autoclaved the whole lab, of course!
Regards,
Terry
Quote:
Terry M said:
I think a Tyvek lab interior lining is a bad idea. The interior surfaces should be sealed, non-porous and easily cleanable. Contaminants enter the lab via you and the door you walk in, not through the walls.
Since Tyvek is highly porous, it would be a bitch to clean. Unless you autoclaved the whole lab, of course!
Regards,
Terry
I agree. Impossible to wipe down. I am also working on a lab and in the same boat. Wondering about green board sheetrock painted with two or three coats of oil based paint. NO way anything is gonna grow on it, non porous and easy to wipe down and the cheapest option for me since the sheetrock is already there. otherwise, it is plastic sheeting. I am using the metal backed half inch foam board in my current setup and it works great. just seal the seams with silicone caulk.
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DRCola
Registered: 05/04/11
Posts: 111
Loc: USA
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Re: Things every lab/clean room should have [Re: chefinainteasy]
#16839918 - 09/13/12 10:34 AM (8 months, 30 days ago) |
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Quote:
chefinainteasy said:
Can you put tyvek suits in the laundry? Or do people not reuse them? Seems like a waste if not.
Ya I bet with using open flame and flammable liquids a fire extinguisher is rather important. Are there any materials, such as wood cabinets, certain surfaces, or anything you can think of that should be avoided?
Hi Chefinainteasy (like the "handle" B.T.W.)
There is a PDF (use Adobe reader) document available on the DuPont website. Here is the link: http://www2.dupont.com/Personal_Protection/en_US/assets/downloads/cleanroom/07_09/Tyvek_IsoClean_Q&A.pdf
Question 8 has the laundry answer. And another one specified flammability.
Hope that's useful.
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OICU812
Chefs Choice Mushroom Co.
Registered: 11/06/11
Posts: 751
Loc: Foothills of NC
Last seen: 3 hours, 57 minutes
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Re: Things every lab/clean room should have [Re: DRCola]
#16842323 - 09/13/12 07:58 PM (8 months, 30 days ago) |
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Prime & paint the walls glossy white (cause it's more cleanable than flat). Wear the tyvek suit when you enter. You will have better luck with contamination that way.
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--------------
"Human beings, who are almost unique in having the ability to learn from the experiences of others,
are also remarkable for their apparent disinclination to do so." - Douglas Adams
“Experience is the best teacher . . . and fools will
learn from no other" - my Grandmother
Forget injuries, never forget kindnesses. - Confucius
|
Amanita virosa
botanist by day
Registered: 12/04/11
Posts: 1,556
Loc: north kakalacky
Last seen: 9 hours, 21 minutes
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Re: Things every lab/clean room should have [Re: OICU812]
#16842553 - 09/13/12 08:42 PM (8 months, 30 days ago) |
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Quote:
OICU812 said:
Prime & paint the walls glossy white (cause it's more cleanable than flat). Wear the tyvek suit when you enter. You will have better luck with contamination that way.
oil or latex? Seems like oil would inhibit any growth better and be more tolerant to solvents
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