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 caesar
Seeking causal knowledge
Registered: 05/08/12
Posts: 164
Loc: Texas
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 How best to isolate Galindoi (ATL#7) 
#16250390 - 05/18/12 04:46 PM (1 year, 4 days ago) |
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I am about to start some plates of Galindoi on agar from a print. Ultimately, I want to get an isolate for max overall stone production. What is the best way to do this? Should I:
A) inoculate lots of jars from the agar plates and clone a stone from the best stone-producing jar at harvest time? If these are from spores, might a single jar contain multiple substrains--making isolation for anything other than size impossible?
B) allow the plates to colonize to the point of sclerotia formation (I've seen lots of pictures of this, but I don't know if I should actually expect sclerotia to form on agar); and select the best at that point.
I've never grown sclerotia. However, before committing lots of jars and cabinet space for 2 or 3 months, I'd really like to isolate a good producer which I know will make the time, effort and resources worthwhile. Does anyone have any experience, wisdom or advice on this? Thanks.
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 36fuckin5
Harder than your husband
Registered: 08/11/03
Posts: 3,096
Loc: Diving into Mystical Terr...
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Re: How best to isolate Galindoi (ATL#7) [Re: caesar] 1
#16250522 - 05/18/12 05:25 PM (1 year, 4 days ago) |
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I've done a fairly good-sized ATL#7 grow, just going for truffles, and had pretty good results just knocking up either corn or WBS with MS solution. So I'd say do that, then pick a good one to clone. This way you get some stones now, and a lot more later.
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caesar
Seeking causal knowledge
Registered: 05/08/12
Posts: 164
Loc: Texas
Last seen: 1 month, 16 days
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Re: How best to isolate Galindoi (ATL#7) [Re: 36fuckin5]
#16250682 - 05/18/12 06:01 PM (1 year, 4 days ago) |
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Quote:
36fuckin5 said:
I've done a fairly good-sized ATL#7 grow, just going for truffles, and had pretty good results just knocking up either corn or WBS with MS solution. So I'd say do that, then pick a good one to clone. This way you get some stones now, and a lot more later.
That is how I originally planned it. I don't know if I'm over-thinking it or what. I just read an awesome post by devilstone about his sclerotia grows, and he says he gets better overall yields not from selecting the largest but by an isolate of a smaller, more consistent stone-producer. I just wonder how you would isolate for that?
I will probably either noc some jars from ms on agar and go on and grow them out, or I'll bite the bullet, make my transfers, isolate single strains and keep them marked in petris while growing them out in separate jars. I know this is the ideal; i was just looking for shortcuts I guess.
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andymc
cocoa beetles from zanzibar
Registered: 11/25/09
Posts: 2,390
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Re: How best to isolate Galindoi (ATL#7) [Re: caesar] 2
#16250690 - 05/18/12 06:03 PM (1 year, 4 days ago) |
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I've learned that there isn't much point in trying to isolate from a multispore situation; you just end up senescing the mycelium without really knowing what you're getting from any given strain.
I'd say, put the spores on agar, get them clean, select wedges from a few strong segments, and start 'multispore' jars with those (perhaps using the wedges in LC for your inoculate). Clone and isolate from any big, fat stones you get.
Ultimately the whole process will take months, and numerous jars, though, if you're really looking for an optimal sclerotia-producing strain.
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caesar
Seeking causal knowledge
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Loc: Texas
Last seen: 1 month, 16 days
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Re: How best to isolate Galindoi (ATL#7) [Re: andymc]
#16251551 - 05/18/12 09:45 PM (1 year, 3 days ago) |
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Thank you for the advice. Yeah, I see this is going to be a big investment in time and effort to select a standout substrain. And I see that the first step is just going to have to be growing them out from MS to see what comes. I think it is worth it, and I am prepared to do it. I see what you mean about senescence in keeping isolates during a "testing" phase, and I see the value of getting stones from each iteration. Thanks to you both for replying. You helped direct me.
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andymc
cocoa beetles from zanzibar
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Re: How best to isolate Galindoi (ATL#7) [Re: caesar]
#16251686 - 05/18/12 10:18 PM (1 year, 3 days ago) |
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 Good luck! 
I like Ps. galindoi 
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If he asks me "Did you have a good time?" I'll say,
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Doc_T
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Loc: Colorado 
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Re: How best to isolate Galindoi (ATL#7) [Re: andymc] 1
#16253325 - 05/19/12 09:45 AM (1 year, 3 days ago) |
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Agreed w/andy, except there's no particular reason to go to LC at the MS stage.
Once upon a time, spores of sclerotia species were rare and exotic, but now not so much.
Spores->agar or grain or cakes, then clone a stone. Use that culture to make more, whether with LC, Grain LC, or whatever method.
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RogerRabbit
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Re: How best to isolate Galindoi (ATL#7) [Re: caesar] 1
#16253399 - 05/19/12 10:18 AM (1 year, 3 days ago) |
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Actually, it's very easy to isolate a super producing sclerotia strain.
Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color. By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.
Now, take each stone and move it to a new dish. Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate. Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR
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caesar
Seeking causal knowledge
Registered: 05/08/12
Posts: 164
Loc: Texas
Last seen: 1 month, 16 days
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Re: How best to isolate Galindoi (ATL#7) [Re: RogerRabbit]
#16253420 - 05/19/12 10:26 AM (1 year, 3 days ago) |
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It's happening. Thank you guys so much. This is an awesome community!
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BobbySkidz
Aces High!
Registered: 02/11/12
Posts: 309
Loc: Neptune
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Re: How best to isolate Galindoi (ATL#7) [Re: RogerRabbit]
#16521678 - 07/12/12 08:50 PM (10 months, 3 days ago) |
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Quote:
RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.
Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color. By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.
Now, take each stone and move it to a new dish. Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate. Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR
Wondering if you would use the same technique described by RR above to isolate a "super slerotia producer" of Mexicana-Strain A as well?
Great info to know!
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