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 Violet
the Clear Light
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 GIVE YOUR TRUFFLES LIGHT *Pics* & most of my knowledge on cultivating these species. 
#16328051 - 06/03/12 09:37 PM (11 months, 12 days ago) |
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Hello there!
This thread is about sclerotia forming species, and how light can optimize their sclerotum growth.
Seems to now also contain much of my knowledge on generally cultivating these.
For those who don't know, here's the good skinny on sclerotia:
Ps. Galindoi [aka Atl#7], Ps. Mexicana, Ps. Tampanensis, Ps. Atlantis. These mushroom species produce in plenty a kind of invitro fruit, sclerotia or "truffles/stones". I think of them as mycelial potatoes. Their purpose serves to live on and rejuvenate the colony in the event of a climate too dry, too wet, too hot or too cold to reproduce or live at all. They are not for reproduction unlike mushroom capophores. The sclerotia form in areas that have become well-consolidated when the colony moves nutrients, water, and metabolites to these locations for storage and defense.
Also unlike capophores, sclerotia are about 2/3 water. Although less potent per non-water mass than mushrooms, their greater relative makeup of fungal body than mushrooms when fresh makes them variably twice [x2] potent as typical Ps. Cubensis. When dried however they lose less water than mushrooms, so the same weight proportion involves many more mushrooms when dried than with fresh, making them variably 2/3 as potent as typical Cubensis.
Best of all, they form in great quantity invitro with extremely high CO2 ppm on nutritious hydrated substrates. This means spawn colonization is almost synonymous with sclerotia 'fruiting'.
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I thus consider these species in fruiting when they are fully colonized.
Now it seems to me that light is immensely beneficial to this fruiting. I have heard/read here that ambient light can have a positive effect on colonization and perhaps on stone formation too, but...
Let's allow the pictures their 1,000 words.
Eleven filled rye jars were inoculated with ~5cc multi-spore GLC Ps. Galindoi on 4-28-12 and immediately shaken to distribute the inoculant. Their colonization was observed for a week after recovery whence they all finished colonization at the same time.
As some began to form stones, I would pick them up and observe closely, but most of them were left most of the time where they sat on a shelf next to my inside-lit mini auto-greenhouse.
It will be obvious, but the photos next to each other are opposite sides of the same jars, the Left being the side facing my GH and the Right the side facing the wall.
 
 
 
All eleven jars could be examples, but these were the clearest to see.
The light they received was indirect, green-tinted, ambient light. Nonetheless, it has made a major obvious impact on sclerotia formation for the positive.
[The green tint is from the light passing thru my greenhouse plastic, I don't use green lighting]
I now thoroughly believe that invitro sclerotia can [perhaps even should] be given light to encourage plentiful, large, fast and strong truffle growth.
Something to consider and experiment:
I know that these species [Galindoi being the one I'm most familiar with] form sclerotia in their casing layers if they don't like their environment when you work to get them to grow mushrooms.
This leads me to believe that they may be more truffle-productive in general when given tolerable environmental stressors.
This means
A) temperature fluxes may support heavier sclerotia yields as well, &
B) a 24/hr light cycle may possibly be further ideal to these ends.
C) Also having grains on the slightly extra-wet side may possibly encourage sclerotia formation, but for sure gives the organism more water with which to make them.
Edited by Violet (07/06/12 02:35 PM)
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 BjJiggles
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 Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16328098 - 06/03/12 09:47 PM (11 months, 12 days ago) |
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Nice comparisons, keep up the research, this is how we progress!! 
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Kizzle
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: BjJiggles]
#16328364 - 06/03/12 10:35 PM (11 months, 12 days ago) |
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I'm curious, not that I have reason to doubt that light is a positive factor, could there be temperature differences at work? The side towards the light might be slightly warmer or something?
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Violet
the Clear Light
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Kizzle]
#16328392 - 06/03/12 10:40 PM (11 months, 12 days ago) |
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Quote:
Kizzle said:
I'm curious, not that I have reason to doubt that light is a positive factor, could there be temperature differences at work? The side towards the light might be slightly warmer or something?
Definitely not, the light is in an enclosed greenhouse that's constantly exchanging air. And as I said in the post the light was dim and diffuse as it hit the jars. Floros emit much less heat also, that's why they're more energy-efficient.
If radiant heat were the case, it would be more apparent in the jars closer to the greenhouse [just 3 or 4] and far less prevalent in the further jars [7 or 8] but it's clear in all 11 jars.
I have 10 more jars reaching full colonization... If I can figure out a way to with all the moving that's about to happen, I intend to give different groups of them no light, diffuse light 12/12, direct flourescent light 12/12, and direct constant flourescent light.
Edited by Violet (06/03/12 10:47 PM)
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TheEaglesGift
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16328487 - 06/03/12 10:59 PM (11 months, 12 days ago) |
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Really interesting. I've yet to grow any of the sclerotia forming species but this has peeked my interest immensely.
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Wing
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16328503 - 06/03/12 11:03 PM (11 months, 12 days ago) |
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Dopsith
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16328527 - 06/03/12 11:09 PM (11 months, 12 days ago) |
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I recently had a mini-mono of Galindoi that refused to fruit for me. It produced very large sclerotia mostly in the casing layer. Harvesting the sclerotia from the sub it became very obvious that it was way too dry to fruit.
I was suprised by the amount of stones I got from the tub with it being so dry (some patches of light and barely moist sub throughout.) I got around 260g of fresh stones.
Quote:
Violet said:
I know that these species [Galindoi being the one I'm most familiar with] form sclerotia in their casing layers if they don't like their environment when you work to get them to grow mushrooms
This quote clears that up for me. I don't know why it wasn't self explanatory before as the assumed genetic purpose of sclerotia is to survive and carry on life where mycelium and fruit bodies cannot.
Very interesting thread, man!
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Dr.Tooty
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dopsith]
#16328722 - 06/03/12 11:47 PM (11 months, 12 days ago) |
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does ATL#7 produce sclerotia well enough with just grain or are there any perks to moving it to a bulk sub?
I'm assuming it's easiest to let a bunch of jars colonize for a month or two and then harvest, though.
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"I get up, I get down." Insect Forum
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Violet
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant [Re: Dopsith]
#16328796 - 06/04/12 12:04 AM (11 months, 12 days ago) |
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Quote:
TootyBooty said:
does ATL#7 produce sclerotia well enough with just grain
You bet your ass it does.
Leave them in their jars for extra months to form a few more stones and really build up their strength.
Quote:
TootyBooty said:
or are there any perks to moving it to a bulk sub?
You bet your ass there are.
Breaking up the grain spawn and removing the harvest-sizable stones is like a "first flush" of stones. Their absence, and the colonization of the new substrate, will inspire the colony to form MORE STONES. Even just straight-casing the grain, my preferred method for stone-producers thusfar, it will form many new ones. The small stones left in will continue to grow too. In fact they'll all form and grow much faster in your fruiting environment than invitro.
And let's not forget, the mushrooms.... keep reading
Quote:
TootyBooty said:
I'm assuming it's easiest to let a bunch of jars colonize for a month or two and then harvest, though.
It's not much more difficult to harvest over a screen mesh of sorts that allows the grain spawn to fall thru into a tray/tub with bulk substrate. You can grow their small but potent mushrooms in plenty, and it will still grow sclerotia in the bulk sub.
Stonesun's "Just As Easy As Cubes" Galindoi monotub.
Give stone-producers lots more fresh air than Cubensis requires.
I recommend the total absence of vermiculite and manure from your substrates.
Quote:
Dopsith said:
I recently had a mini-mono of Galindoi that refused to fruit for me. It produced very large sclerotia mostly in the casing layer. Harvesting the sclerotia from the sub it became very obvious that it was way too dry to fruit.
This quote clears that up for me. I don't know why it wasn't self explanatory before as the assumed genetic purpose of sclerotia is to survive and carry on life where mycelium and fruit bodies cannot.
I recently used this trait of theirs with a small casing that had flushed once already to fine-tune my automated fruiting environment between the wet&cold/dry&hot extremes. For being such a tiny tray, it has four obvious stone protrusions.
After fine-tuning, it has pinned again and is heartily growing a few med-large fruits  Xmas in June
Edited by Violet (06/04/12 12:19 AM)
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funkyfish77
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dr.Tooty]
#16328870 - 06/04/12 12:17 AM (11 months, 12 days ago) |
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How did you know I was thinking about trying some truffles. Jars look great !, maybe one day
I'll have my own stones to look at until then I'll look at yours . Nice stones
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Nice tubs Here
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Dr.Tooty
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dr.Tooty]
#16328891 - 06/04/12 12:21 AM (11 months, 12 days ago) |
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I don't have access to manure currently and am stuck with coir.
I don't think they will fruit on coir...but what about stone production, do you think that would be affected?
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"I get up, I get down." Insect Forum
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Violet
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dr.Tooty]
#16328914 - 06/04/12 12:26 AM (11 months, 12 days ago) |
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Quote:
TootyBooty said:
I don't have access to manure currently and am stuck with coir.
I don't think they will fruit on coir...but what about stone production, do you think that would be affected?
Which "they" are you referring to? Galindoi also?
They will fruit on coir. They'll fruit on straight-cased grain. THEY WILL FRUIT. Most bountiful organism EVER, that Galindoi.
I'm pretty sure the others will too.
As I said above, I recommend the complete absence of manure and vermiculite from their substrates. This is because I know peeps want the bulk sub sclerotia. They'll make lots.
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Dr.Tooty
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16328967 - 06/04/12 12:39 AM (11 months, 12 days ago) |
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Quote:
Violet said:
Which "they" are you referring to? Galindoi also?
They will fruit on coir. They'll fruit on straight-cased grain. THEY WILL FRUIT. Most bountiful organism EVER, that Galindoi.
Yes, sorry, I did mean Galindoi.
and sorry, I completely misread the part saying to leave out verm and manure. I just took my first few hits of the night and am feeling very relaxed.
This is awesome to know! I now have a new project to look forward to!
Deep tubs + lots of spawn/sub = Stones and  !
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"I get up, I get down." Insect Forum
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Violet
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dr.Tooty]
#16329019 - 06/04/12 12:47 AM (11 months, 12 days ago) |
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Quote:
TootyBooty said:
This is awesome to know! I now have a new project to look forward to!
Deep tubs + lots of spawn/sub = Stones and !
The mushrooms are small, but numerous. Really a sub of 3" is well more than enough. It will hold more water than all the flushes will need, though I've gently injected clean water into trays and I suppose it may help the yield between mushrooms and stones.
Yes it will flush multiple times, nicely.
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Dr.Tooty
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16329046 - 06/04/12 12:53 AM (11 months, 12 days ago) |
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Quote:
Violet said:
Quote:
TootyBooty said:
This is awesome to know! I now have a new project to look forward to!
Deep tubs + lots of spawn/sub = Stones and !
The mushrooms are small, but numerous. Really a sub of 3" is well more than enough. It will hold more water than all the flushes will need, though I've gently injected clean water into trays and I suppose it may help the yield between mushrooms and stones.
Yes it will flush multiple times, nicely.
These tubs are about the same width and length as mine, but a lot deeper, so i figure it might help the stone production while giving me about the same amount of fruits.
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"I get up, I get down." Insect Forum
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Violet
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dr.Tooty]
#16329095 - 06/04/12 01:04 AM (11 months, 12 days ago) |
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Quote:
TootyBooty said:
Quote:
Violet said:
Quote:
TootyBooty said:
This is awesome to know! I now have a new project to look forward to!
Deep tubs + lots of spawn/sub = Stones and !
The mushrooms are small, but numerous. Really a sub of 3" is well more than enough. It will hold more water than all the flushes will need, though I've gently injected clean water into trays and I suppose it may help the yield between mushrooms and stones.
Yes it will flush multiple times, nicely.
These tubs are about the same width and length as mine, but a lot deeper, so i figure it might help the stone production while giving me about the same amount of fruits.
Yeah it will. You'll get more fruits that way too for sure, as in your second flushes and on will be rather larger than otherwise. If it were mushroom-only mycelium it would be an inefficient use of substrate... but, STONES! ;] ;] ;] ;] ;]
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Dr.Tooty
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16329128 - 06/04/12 01:11 AM (11 months, 12 days ago) |
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doing it!
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"I get up, I get down." Insect Forum
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Violet
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Dr.Tooty]
#16331494 - 06/04/12 04:20 PM (11 months, 11 days ago) |
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Bringin' it back up for today's viewers 
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caesar
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: Violet]
#16331657 - 06/04/12 05:09 PM (11 months, 11 days ago) |
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More great knowledge on Galindoi, Violet, thank you. Please post the results of your next "light trial!"
I have 3 questions which I bet you can help with:
#1, Have you had good luck with multi-spore on Galindoi? Should I worry that I'll waste a crop cycle by not first isolating for stone production on agar?
#2, RR mentioned that master cultures of Mexicana A fared poorly in normal refrigeration temperatures. Have you noticed any cold aversion in Galindoi in either LC's or master cultures? How would you suggest storing them medium to long-term?
#3, I have read in several places that sclerotia seem to form most commonly against the walls of their containers. Have you found this to be true? If so, have you tried any strategies to capitalize on its affinity for forming stones at boundary areas?
I've found you a wealth of information in the past, and I'm loving the experiments with Galindoi. Keep up the great work and keep sharing, please!
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 deucedbi9
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Re: GIVE YOUR TRUFFLES LIGHT - Your sclerotia species is in constant "fruiting"! *Pics* [Re: caesar]
#16331722 - 06/04/12 05:29 PM (11 months, 11 days ago) |
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Quote:
caesar said:
More great knowledge on Galindoi, Violet, thank you. Please post the results of your next "light trial!"
I have 3 questions which I bet you can help with:
#1, Have you had good luck with multi-spore on Galindoi? Should I worry that I'll waste a crop cycle by not first isolating for stone production on agar?
#2, RR mentioned that master cultures of Mexicana A fared poorly in normal refrigeration temperatures. Have you noticed any cold aversion in Galindoi in either LC's or master cultures? How would you suggest storing them medium to long-term?
#3, I have read in several places that sclerotia seem to form most commonly against the walls of their containers. Have you found this to be true? If so, have you tried any strategies to capitalize on its affinity for forming stones at boundary areas?
I've found you a wealth of information in the past, and I'm loving the experiments with Galindoi. Keep up the great work and keep sharing, please!
#1. I've found MS to work well enough, but I'm sure isolating from a good stone would be even better.
#2. You could use dry grains from a 'stone grow' for later rejuvenation.
Something a little less haphazard than this> Atl#7 dried corn.
#3. I think it may be that the containers have glass walls, so, that is where you see them.
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