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 mrx8110
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Registered: 05/20/12
Posts: 6
Last seen: 11 months, 7 days
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 brf substrate turning grey 
#16257096 - 05/20/12 08:50 AM (1 year, 2 days ago) |
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 Ive been using the pf-tek with brf and vermiculite, its been 10 days since i inoculated my jars...
this one waz the last one to be inoculated and got very little spore water...how ever do u see the dark area on the brf...is that normal or is it some kind of contamination? all my jars got that grey on the brf but the mycelium is growing over it..
Edited by mrx8110 (05/20/12 08:53 AM)
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 Doc_T
Random Dude
 
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
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Re: brf substrate turning grey [Re: mrx8110]
#16257115 - 05/20/12 09:05 AM (1 year, 2 days ago) |
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Looks contaminated. the white stuff is what you want.
Next time inject your sore solution all down the side, not just at the top.
And a broader, shorter glass will work better than a tall one. I guess you aren't in the US?
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mrx8110
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Last seen: 11 months, 7 days
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Re: brf substrate turning grey [Re: Doc_T]
#16257126 - 05/20/12 09:11 AM (1 year, 2 days ago) |
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i did that on my other jars,but after 3-4 days u could see that brf turning grey. and no im not in the us. aroud the inoculation points
Edited by mrx8110 (05/20/12 09:12 AM)
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: brf substrate turning grey [Re: mrx8110]
#16257136 - 05/20/12 09:17 AM (1 year, 2 days ago) |
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If it turns grey around the inoculation points you probably have a contaminated syringe.
Next time, leave one jar unshot. If it turns grey, your sterilization isn''t working.
If that jar stays good and the ones you inoculate turn bad, it's a bad syringe.
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You make it all possible. Doesn't it feel good?
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mrx8110
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Registered: 05/20/12
Posts: 6
Last seen: 11 months, 7 days
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Re: brf substrate turning grey [Re: Doc_T]
#16257145 - 05/20/12 09:23 AM (1 year, 2 days ago) |
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ok i got it...better order some spore prints then...and thanks for the fast answer..
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: brf substrate turning grey [Re: mrx8110]
#16257150 - 05/20/12 09:24 AM (1 year, 2 days ago) |
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Prints are better than syringes for sure, you can make 100 syringes from one print.
And start looking around for a pressure cooker. Canning is a US hobby, but there are pressure cookers worldwide.
Agar work is cheaper and easier than it looks, but you need a pressure cooker.
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You make it all possible. Doesn't it feel good?
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mrx8110
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Registered: 05/20/12
Posts: 6
Last seen: 11 months, 7 days
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 Re: brf substrate turning grey [Re: Doc_T]
#16257190 - 05/20/12 09:48 AM (1 year, 2 days ago) |
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yeah for sure...i tried out of spore prints this time as well..its my first attempt ever to grow shrooms....but tried to sterilize with just the lid on for 90 minutes...but i made the spore water sterilization different...at least the cup i used to blend spores with water..so its probably the reason for contamination..but still i need a pc for sure...i would prefer a syringe over a print just because its easy for my first grow but i defiantly learn more by doing it all from scratch...but i have learned alot just using this forum...and i can assure u that i will post here again...next time with a better result i hope  and thanks again
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: brf substrate turning grey [Re: mrx8110]
#16257201 - 05/20/12 09:52 AM (1 year, 2 days ago) |
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Go easy on the spores, a tiny flake is all you need in a syringe. 
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You make it all possible. Doesn't it feel good?
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mrx8110
Stranger
Registered: 05/20/12
Posts: 6
Last seen: 11 months, 7 days
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Re: brf substrate turning grey [Re: Doc_T]
#16257218 - 05/20/12 10:01 AM (1 year, 2 days ago) |
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oh i use a thumb size spore print for 10cc water
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: brf substrate turning grey [Re: mrx8110]
#16257219 - 05/20/12 10:02 AM (1 year, 2 days ago) |
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Yes, that's about 100x too rich.
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You make it all possible. Doesn't it feel good?
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mrx8110
Stranger
Registered: 05/20/12
Posts: 6
Last seen: 11 months, 7 days
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Re: brf substrate turning grey [Re: Doc_T]
#16257239 - 05/20/12 10:09 AM (1 year, 2 days ago) |
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w.o.w u learn something new everyday..im gonna keep that in mind for next time...i got a little spores left on the tinfoil...maybe i can use that...or maybe just order a new one in case i contaminated the spore print
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