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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: hello i need a bit of help [Re: mr.coolguy]
#16223248 - 05/13/12 09:32 AM (1 year, 7 days ago) |
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You probably have bacterial contamination in one or more jars. It won't spread from jar to jar, so there's no special need to isolate it. When you figure out which of the jars is stinky, toss it. (If it was all of them, it would smell worse)
Did you go get the correct jars yet? These tall ones are going to keep giving you trouble.
-------------------- You make it all possible. Doesn't it feel good?
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
Last seen: 4 days, 2 hours
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Re: hello i need a bit of help [Re: mr.coolguy]
#16223249 - 05/13/12 09:33 AM (1 year, 7 days ago) |
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Quote:
i would say a fruity fermentation smell
That indicates bacteria contamination. Stop right here and start over, following the tek to the letter. This way, you're sure to have fresh mushrooms in six weeks. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
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mr.coolguy
Stranger


Registered: 03/04/12
Posts: 106
Last seen: 10 months, 4 days
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Re: hello i need a bit of help [Re: Doc_T]
#16223260 - 05/13/12 09:42 AM (1 year, 7 days ago) |
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Quote:
Doc_T said: You probably have bacterial contamination in one or more jars. It won't spread from jar to jar, so there's no special need to isolate it. When you figure out which of the jars is stinky, toss it. (If it was all of them, it would smell worse)
Did you go get the correct jars yet? These tall ones are going to keep giving you trouble.
no i didnt im going to wait till i try again of if any of these make it i want to do a LC or some kind of transfer maybe clone a fruit body but i cant find any petri dishes that can be heat sterilized
-------------------- those who were meant to smell the roses don't mind the thorns
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mr.coolguy
Stranger


Registered: 03/04/12
Posts: 106
Last seen: 10 months, 4 days
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Quote:
RogerRabbit said:
Quote:
i would say a fruity fermentation smell
That indicates bacteria contamination. Stop right here and start over, following the tek to the letter. This way, you're sure to have fresh mushrooms in six weeks. RR
by start over do you mean toss all my jars and what do mushrooms smell like also my room is 70 to 76 degrees
-------------------- those who were meant to smell the roses don't mind the thorns
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: hello i need a bit of help [Re: mr.coolguy]
#16223267 - 05/13/12 09:45 AM (1 year, 7 days ago) |
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LC sucks, forget you ever heard about it. If you try to use an LC method, YOU WILL FAIL. Understand?
If you go get the correct PF jars, you can use those for agar until you buy petri dishes. Plus, like RR says, you need to follow the tek, and the right jars are part of the tek.
-------------------- You make it all possible. Doesn't it feel good?
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mr.coolguy
Stranger


Registered: 03/04/12
Posts: 106
Last seen: 10 months, 4 days
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Re: hello i need a bit of help [Re: Doc_T]
#16223276 - 05/13/12 09:53 AM (1 year, 7 days ago) |
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Quote:
Doc_T said: LC sucks, forget you ever heard about it. If you try to use an LC method, YOU WILL FAIL. Understand?
If you go get the correct PF jars, you can use those for agar until you buy petri dishes. Plus, like RR says, you need to follow the tek, and the right jars are part of the tek.
yeah i kn ow i just thought that if they were the same volume it would work but in good news one jar looks great the myc covers most of the jar and its moving down to the bottom
-------------------- those who were meant to smell the roses don't mind the thorns
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: hello i need a bit of help [Re: mr.coolguy]
#16223329 - 05/13/12 10:20 AM (1 year, 7 days ago) |
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It's not impossible, people have used the taller jars for cakes, including me. But the widemouths work better, and you want every advantage you can get.
And truly, LC is a waste of your time. Don't even bother.
-------------------- You make it all possible. Doesn't it feel good?
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mr.coolguy
Stranger


Registered: 03/04/12
Posts: 106
Last seen: 10 months, 4 days
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Re: hello i need a bit of help [Re: Doc_T]
#16223358 - 05/13/12 10:35 AM (1 year, 7 days ago) |
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Quote:
Doc_T said: LC sucks, forget you ever heard about it. If you try to use an LC method, YOU WILL FAIL. Understand?
If you go get the correct PF jars, you can use those for agar until you buy petri dishes. Plus, like RR says, you need to follow the tek, and the right jars are part of the tek.
Quote:
Doc_T said: It's not impossible, people have used the taller jars for cakes, including me. But the widemouths work better, and you want every advantage you can get.
And truly, LC is a waste of your time. Don't even bother.
ok thanks but what should i do to clone and inoculate more jars also i spore printed wild mushrooms before but how should i spore print a weak indoor one i just cant think of any way to sterilize it with out killing the spores
-------------------- those who were meant to smell the roses don't mind the thorns
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: hello i need a bit of help [Re: mr.coolguy]
#16223364 - 05/13/12 10:38 AM (1 year, 7 days ago) |
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Clone using a cloneing tek with agar. RR shows it in his video. Wild spores will be contaminated, you'll have to clean those up on agar. Your current syringe seems to be contaminated, you could clean that on agar.
I'm thinking you need agar.
-------------------- You make it all possible. Doesn't it feel good?
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mr.coolguy
Stranger


Registered: 03/04/12
Posts: 106
Last seen: 10 months, 4 days
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Re: hello i need a bit of help [Re: Doc_T]
#16223385 - 05/13/12 10:43 AM (1 year, 7 days ago) |
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Quote:
Doc_T said:
I'm thinking you need agar.
OH YEAH but i dont know how to mix it how much to put in the dish or any of that i watched some vids but they dont show everything
-------------------- those who were meant to smell the roses don't mind the thorns
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: hello i need a bit of help [Re: mr.coolguy]
#16223390 - 05/13/12 10:46 AM (1 year, 7 days ago) |
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Well, you've got some time to research that.
-------------------- You make it all possible. Doesn't it feel good?
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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Re: hello i need a bit of help [Re: mr.coolguy]
#16224714 - 05/13/12 03:54 PM (1 year, 7 days ago) |
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mr.coolguy, I gave you a bit of a hard time earlier in your thread. I'm not trying to be difficult (I can be at times). But I do want to see you succeed with at least one brf (that is what your doing? brf?)I think the experience is tremendous (it definitely is something to see after all the hard work and time you will put into this). So keep trying and don't give up. You will get this all figured out @ Doc_T, I'm thinking about stealing boxxy from you. You don't mind, right?
--------------------
If it's anonymous you want... IRC: irc.apexsec.ca SSL port: +6697 Room #Apexsec
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otaku on shrooms
otaku on shrooms



Registered: 05/04/12
Posts: 335
Loc: arizona usa
Last seen: 8 months, 10 days
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um you dont need agar to clean up your syringe just soak it emerged in bleach for 24 hours purge with boiling water repeatedly then wrap with tin foil and steam for about an hour you'll be good
-------------------- im not r kelly i do not create evidence, and my experments are purely theoretical i refuse to post pics of any grows so ethier take my words at face value or simply choose to think what you already do. please note its my experiance that i dont think like others so please be patiant when i have to explain something it may make no sense to you but it does to me
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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I believe your talking about two different things here. He can do agar work to clean up his syringe if it's contamed by putting some of the spores from the syringe to agar. Both will grow, then he can cut out the good part and put that on an other petri dish. This will get ride of the contam. And your talking about sterilizing a syringe.
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If it's anonymous you want... IRC: irc.apexsec.ca SSL port: +6697 Room #Apexsec
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Ill-bird


Registered: 12/14/11
Posts: 1,563
Loc: AMERICA FUCK YEAH
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Hot boxxy, mmm mmm. I'd like to plug her tap hole if ya know what I mean. LOL
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smitty5606
country boy



Registered: 01/07/12
Posts: 848
Loc: southern states
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You should specify that your talking about a empty syringe..because bleach will kill your spores
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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Re: hello i need a bit of help [Re: Ill-bird]
#16228158 - 05/14/12 10:48 AM (1 year, 6 days ago) |
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Careful there lll-bird!! Boxxy can get to be a bit of a touchy subject for Doc_T. As seen here at the bottom
Quote:
MagicCarpetRide89 said: @ Doc_T, Still waiting for an answer on Boxxy
And he responded back with this
Quote:
Doc_T said:
Quote:
MagicCarpetRide89 said: @ Doc_T, Still waiting for an answer on your ignore list
You could end up there.
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If it's anonymous you want... IRC: irc.apexsec.ca SSL port: +6697 Room #Apexsec
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Actually, it's attention whoring that irks me. You've been trying to push my buttons to see if I'd react. Well, ok then. I'll react so you can STFU.
-------------------- You make it all possible. Doesn't it feel good?
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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Re: hello i need a bit of help [Re: Doc_T]
#16228197 - 05/14/12 11:04 AM (1 year, 6 days ago) |
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Why are you taking this so serious? Is it wrong to have a little fun?? I personally don't think I said anything that bad but if you wana put me on your ignore list, I can't stop you
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If it's anonymous you want... IRC: irc.apexsec.ca SSL port: +6697 Room #Apexsec
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smitty5606
country boy



Registered: 01/07/12
Posts: 848
Loc: southern states
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He wants to be her only stalker, let him be
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