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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Gypsum, Rye Grain

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Offlinebootsyyy
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No Growth RYE - PICS
    #16171938 - 05/02/12 05:55 PM (1 year, 11 months ago)

Hey everyone, i shot up these RYE Jars about 7 days ago with multispore from sporeworks and dont have any signs of growth, but also no sign of contams.

This is my first time using RYE, but with BRF and Popcorn ive never had a problem, or even a contam for that matter

I soaked for 24 hours then followed the "rye bitches rye" tek and boiled for 10 minutes before rinse and PC. I also made sure that the RYE was not too wet before loading into the jars. Tyvek lids also. Im kinda getting worried about them now since i usually always have growth within 7 days. Could it be the RYE? Or should i just keep waiting?



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OfflineDiesel_Dawg
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16171989 - 05/02/12 06:09 PM (1 year, 11 months ago)

Sporeworks is obviously sending out bunk syringes. Because I think its more than coincidence that three of us here are having the exact same problem with the exact same vendor.


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Invisiblecubes4cancer
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Re: No Growth RYE - PICS [Re: Diesel_Dawg]
    #16172002 - 05/02/12 06:13 PM (1 year, 11 months ago)

if nothing comes to be in another week start :cussing:


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$$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
                                        :ramen:



ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc.
In other words,it's hard to fuck up a cube grow.
RR


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OfflineDiesel_Dawg
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Re: No Growth RYE - PICS [Re: cubes4cancer]
    #16172041 - 05/02/12 06:23 PM (1 year, 11 months ago)

Thats the shitty part, by purchasing the spores you are entering into a legal contract saying you will not germinate them under any circumstances, so they can get away with it. I am still at the conclusion stage, but if I get any more confirmation on this, I'm changing vendors for sure.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: cubes4cancer]
    #16172042 - 05/02/12 06:23 PM (1 year, 11 months ago)

really? thats crazy, my syringe's i got are FULL of spores, very dark and i made sure to shake all of them before hand.
Quote:

Diesel_Dawg said:
Sporeworks is obviously sending out bunk syringes. Because I think its more than coincidence that three of us here are having the exact same problem with the exact same vendor.




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OfflineDiesel_Dawg
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16172049 - 05/02/12 06:24 PM (1 year, 11 months ago)

Mine are the same way, bro. Which is different, because they used to send me some pretty clear syringes, but they were always viable, now all of the sudden they are dark and don't work. Very suspicious.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: Diesel_Dawg]
    #16172059 - 05/02/12 06:26 PM (1 year, 11 months ago)

oh so you just recently got some and they didnt work?


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16172406 - 05/02/12 07:36 PM (1 year, 11 months ago)

does anyone els have an opinion on what is happening here? I thought sporeworks was suppose to be awesome.


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Offlinerohr
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Re: No Growth RYE - PICS [Re: cubes4cancer] * 1
    #16172658 - 05/02/12 08:19 PM (1 year, 11 months ago)

Quote:

cubes4cancer said:
if nothing comes to be in another week start :cussing:




I second this. Seven days really isn't long for multispore. I don't think it's time to get upset yet, but if you're really worried, perhaps you could get started on some more jars so you'll have something to fall back on?

I've only ever purchased from ____________-. Since my first experience was so positive, I never had a reason to go elsewhere. I have a syringe left from when I ordered during the Halloween special, I really need to use that bad boy up real soon. The syringes I did use from the Halloween special germinated beautifully, save for one. Mean green hit me with the Costa Rica attempt, but the moisture content was far too high in those jars, so easily could have been my fault.

I've got Hautla spores in seven rye jars now, also from the Halloween pack. Inoculated Saturday. After checking on them this morning, I believe I have germination in three of them. But that's pretty fast for germination in my experience, so I'm not going to say for certain that germination took place since it's too early to tell if it's myc or the goddamn green again. I'm staying positive and I'll report back when I can say for certain whether it's green or myc since there seems to be rumors of foul play afoot.

:tripping:


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Edited by Prisoner#1 (05/02/12 08:39 PM)


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: rohr]
    #16172742 - 05/02/12 08:34 PM (1 year, 11 months ago)

Okay ill wait a bit longer. I have a STRONG feeling that it is the spores though, So i actually contacted sporeworks and they are sending me some replacements. I also gave them the "batch number" so they can pinpoint if some of there stuff is bad.


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OfflineDrCrumbs
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16173134 - 05/02/12 09:30 PM (1 year, 11 months ago)

Did you shake it after you inoculated it?

How many cc's did you use per jar?

It can be hard to get Spores to germinate on strait grain, using an LC or agar is going to ensure success.


They aren't bunch trust me, they look great under the scope.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16173152 - 05/02/12 09:32 PM (1 year, 11 months ago)

i did NOT shake after, mainly since i used multispore, and i dont think thats the problem at all. 2ccs in each quart jar. I did these the exact same way i did all of my popcorn and BRF.


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OfflineDrCrumbs
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16173165 - 05/02/12 09:33 PM (1 year, 11 months ago)

Sounds good, I would have expected results also. I have had problems germinating on grain though and LC's made it work.


Edited by DrCrumbs (05/02/12 09:34 PM)


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16186505 - 05/05/12 05:13 PM (1 year, 11 months ago)

Im confused now, 3 of my jars are now showing a VERY VERY TINY amount of growth, Im not sure if these spores were just weak or what.


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InvisibleBobbySkidz
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Re: No Growth RYE - PICS *DELETED* [Re: bootsyyy]
    #16189198 - 05/06/12 08:16 AM (1 year, 11 months ago)

Post deleted by BobbySkidz

Reason for deletion: .



Edited by BobbySkidz (05/06/12 08:16 AM)


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OfflineDazed Belief
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Re: No Growth RYE - PICS [Re: BobbySkidz]
    #16189322 - 05/06/12 09:12 AM (1 year, 11 months ago)

first off i would say you should remove the vendors name
no need to get them in trouble for you illegal actions

second off they are a very good vendor how many people on this site trust
myself as on of them, i would not use anyone else

and third off dont just start bashing a vendor when more then likely its something you did
there are many variables in this process and if one of them is off it could mess up your whole grow


--------------------
Enjoy the dazed state of life.
Everything i say or do on this page is all fake.
im just trying to live the life.


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Offlinedeucedbi9
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189559 - 05/06/12 10:56 AM (1 year, 11 months ago)

Quote:

bootsyyy said:






How did you make the lid you used. Number/size of holes.
If they're not drying out from too many/large holes, you should be OK as it looks like there's a small amount of myc growth at the bottom, unless it's a trick of the light, so you should be seeing more by now.


--------------------
Cheap coir in the UK from £0.88p (inc p+p) per brick. Link


whether low pressure sucks or high pressure blows...
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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: deucedbi9]
    #16189566 - 05/06/12 10:59 AM (1 year, 11 months ago)

i have 2 holes, one very small one to inoculate and a 1/2" hole for FAE, i followed the "RYE BITCHES RYE" tek to a T.

that is a trick of the light, but i do now have a small amount of growth.


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OfflineGoldenArrow
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Re: No Growth RYE - PICS [Re: deucedbi9]
    #16189567 - 05/06/12 11:00 AM (1 year, 11 months ago)

I've got a similar issue, rye I put cube spores on on the 16th of April and the jars look exactly the same as day 1...not sure what's going on!


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: Dazed Belief]
    #16189572 - 05/06/12 11:01 AM (1 year, 11 months ago)

Quote:

Dazed Belief said:
first off i would say you should remove the vendors name
no need to get them in trouble for you illegal actions

second off they are a very good vendor how many people on this site trust
myself as on of them, i would not use anyone else

and third off dont just start bashing a vendor when more then likely its something you did
there are many variables in this process and if one of them is off it could mess up your whole grow




Im not bashing this vendor at all, they have great customer service, but latley a few of us have had the EXACT same problem with not getting any growth.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: BobbySkidz]
    #16189573 - 05/06/12 11:02 AM (1 year, 11 months ago)

Quote:

BobbySkidz said:
May I ask what strain this was?





Golden Teacher


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OfflineDazed Belief
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189637 - 05/06/12 11:25 AM (1 year, 11 months ago)

Quote:

bootsyyy said:
Quote:

Dazed Belief said:
first off i would say you should remove the vendors name
no need to get them in trouble for you illegal actions

second off they are a very good vendor how many people on this site trust
myself as on of them, i would not use anyone else

and third off dont just start bashing a vendor when more then likely its something you did
there are many variables in this process and if one of them is off it could mess up your whole grow




Im not bashing this vendor at all, they have great customer service, but latley a few of us have had the EXACT same problem with not getting any growth.




yes there are a lot of people that have this problem with all vendors
each syringe has millions of spores and for none of them to germinate is a little hard to believe
and i dont see any vendor doing anything that would hurt the integrity of a syringe
usually is the people growing it doing something wrong

and a 1/2in whole is pretty big man
i only use a 1/4in


--------------------
Enjoy the dazed state of life.
Everything i say or do on this page is all fake.
im just trying to live the life.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: Dazed Belief]
    #16189679 - 05/06/12 11:39 AM (1 year, 11 months ago)

Quote:

Dazed Belief said:
Quote:

bootsyyy said:
Quote:

Dazed Belief said:
first off i would say you should remove the vendors name
no need to get them in trouble for you illegal actions

second off they are a very good vendor how many people on this site trust
myself as on of them, i would not use anyone else

and third off dont just start bashing a vendor when more then likely its something you did
there are many variables in this process and if one of them is off it could mess up your whole grow




Im not bashing this vendor at all, they have great customer service, but latley a few of us have had the EXACT same problem with not getting any growth.




yes there are a lot of people that have this problem with all vendors
each syringe has millions of spores and for none of them to germinate is a little hard to believe
and i dont see any vendor doing anything that would hurt the integrity of a syringe
usually is the people growing it doing something wrong

and a 1/2in whole is pretty big man
i only use a 1/4in




Thanks for the info, I have to disagree a little though, ive spoken to a few people this week that recently got spores from the same place i just got mine from.

ive done Popcorn this way and BRF multiple times with no problems. The thing that  stuck out to me is a member got a few different strains from the same place and only one of the strains colonized, all the others had no growth at all. That leads me to believe that this is a freak incident with this vendor.

read this thread: http://www.shroomery.org/forums/showflat.php/Number/16171889


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R O X I


Edited by bootsyyy (05/06/12 11:40 AM)


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OfflineDazed Belief
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189715 - 05/06/12 11:47 AM (1 year, 11 months ago)

if you saying last week
then yea you might actually be right
there was that shit storm in the use that snowed real but and temps in some places got below zero
if being shipped with temp being that low they would be a chance of the syringes turned to shit
probably to cold for them


--------------------
Enjoy the dazed state of life.
Everything i say or do on this page is all fake.
im just trying to live the life.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189719 - 05/06/12 11:48 AM (1 year, 11 months ago)

yep, so ill post with the new stuff and see how it goes


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Offlinedeucedbi9
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189737 - 05/06/12 11:53 AM (1 year, 11 months ago)

Quote:

bootsyyy said:
i have 2 holes, one very small one to inoculate and a 1/2" hole for FAE, i followed the "RYE BITCHES RYE" tek to a T.

that is a trick of the light, but i do now have a small amount of growth.




Two holes? I don't know the Tek, but I'm surprised it suggested a 1/2" hole for "FAE":eek: as that is likely the problem. Your grain is drying out.
One 1/4" hole, covered with micro-pore tape after inoculation, would suffice.


--------------------
Cheap coir in the UK from £0.88p (inc p+p) per brick. Link


whether low pressure sucks or high pressure blows...
it's a bugger to cycle in


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OfflineDazed Belief
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189761 - 05/06/12 11:59 AM (1 year, 11 months ago)

Quote:

bootsyyy said:
yep, so ill post with the new stuff and see how it goes




ok good shit man
i want trying to be a dick man
hope eveything works out for ya


--------------------
Enjoy the dazed state of life.
Everything i say or do on this page is all fake.
im just trying to live the life.


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Offlinerohr
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16189942 - 05/06/12 01:00 PM (1 year, 11 months ago)

Quote:

bootsyyy said:
Im confused now, 3 of my jars are now showing a VERY VERY TINY amount of growth, Im not sure if these spores were just weak or what.




Well, you did use a MS syringe. Rollin dice, you know.

Earlier in the thread I said I'd report back about my grain jars. They've got mycelium in em, I'm certain of it.


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Offlinebootsyyy
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Re: No Growth RYE - PICS [Re: deucedbi9]
    #16189994 - 05/06/12 01:18 PM (1 year, 11 months ago)

Quote:

deucedbi9 said:
Quote:

bootsyyy said:
i have 2 holes, one very small one to inoculate and a 1/2" hole for FAE, i followed the "RYE BITCHES RYE" tek to a T.

that is a trick of the light, but i do now have a small amount of growth.




Two holes? I don't know the Tek, but I'm surprised it suggested a 1/2" hole for "FAE":eek: as that is likely the problem. Your grain is drying out.
One 1/4" hole, covered with micro-pore tape after inoculation, would suffice.




i followed the "RYE BITCHES RYE" tek, check out the picture of the lid in this thread, http://www.shroomery.org/forums/showflat.php/Number/4576865/fpart/1/vc/1

they seem to work fine for him


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R O X I


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Offlinejoyless
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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16190437 - 05/06/12 03:30 PM (1 year, 11 months ago)

maybe its just light, but i think your grain looks really dry


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Re: No Growth RYE - PICS [Re: joyless]
    #16190830 - 05/06/12 05:31 PM (1 year, 11 months ago)

I somehow screwd up the moisture content of my rye jars and I had the inject some cc's of boiled (then cooled) water into them. They colonized well after that.


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OfflineGoldenArrow
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Re: No Growth RYE - PICS [Re: Waldfried]
    #16190863 - 05/06/12 05:44 PM (1 year, 11 months ago)

Since this thread seems to be getting replies and my thread doesn't, can I hijack to ask - are mine too dry then?



I thought that might be the case but then I read a post by RR saying (in essence) "They're almost never too dry, the moisture is in the grains, it's better to be too dry than too wet" so I abandoned my plans to inject some sterilised water...


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OfflineDazed Belief
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Re: No Growth RYE - PICS [Re: GoldenArrow]
    #16190939 - 05/06/12 06:04 PM (1 year, 11 months ago)

Quote:

GoldenArrow said:
Since this thread seems to be getting replies and my thread doesn't, can I hijack to ask - are mine too dry then?



I thought that might be the case but then I read a post by RR saying (in essence) "They're almost never too dry, the moisture is in the grains, it's better to be too dry than too wet" so I abandoned my plans to inject some sterilised water...




yea ur jars look good
and after a 24 hour soak its pretty hard to dry ur grains out to much
not sure about rye though but i would think it the same as everything else


--------------------
Enjoy the dazed state of life.
Everything i say or do on this page is all fake.
im just trying to live the life.


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Re: No Growth RYE - PICS [Re: Dazed Belief]
    #16190997 - 05/06/12 06:20 PM (1 year, 11 months ago)

I'll keep waiting then! Oh, I also put a pinch of gypsum in there so they might look drier than they normally would.


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OfflineDrCrumbs
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Re: No Growth RYE - PICS [Re: GoldenArrow]
    #16197020 - 05/07/12 11:04 PM (1 year, 11 months ago)

Good to see your getting some growth. Rye is much harder to achieve germination on than brf cakes. I bet if you took that same syringe and put it on Agar/LC or a cake you would have growth.

Best of luck


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OfflineGoldenArrow
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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16214786 - 05/11/12 01:57 PM (1 year, 11 months ago)

If anyone is keeping score, I've got growth now too! A tiny dot....possibly a record, 4 weeks after inoculation? Highly doubtful it's mycellium, but you never know! Now to see if my sclerotia jar will ever start...


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OfflineDrCrumbs
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Re: No Growth RYE - PICS [Re: GoldenArrow]
    #16215029 - 05/11/12 02:54 PM (1 year, 11 months ago)

It probably is mycelium, best of luck!


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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16215056 - 05/11/12 03:02 PM (1 year, 11 months ago)

my jars started colonizing, the spores were just weak i guess, cause i got some more from the same vendor, and they started growing after 3 days.


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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16215057 - 05/11/12 03:02 PM (1 year, 11 months ago)

While the grains do look a tad dry, there still lots of moisture in those kernels, should be enough for the mycelium to grow. Sometimes spores can be slow to germinate and what does germinate might be some slow growing strains, especially if they are old, which you never know they could be.


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Re: No Growth RYE - PICS [Re: tubervegan]
    #16215063 - 05/11/12 03:04 PM (1 year, 11 months ago)

yea, it was kinda weird because I posted this thread and found out that alot of other people had problems lately with the same vendor, and it took 2 weeks for them to start, but now they are going fine. Ill post some pics of the grow soon.

The vendor was nice enough to send me new spores and like i said they started growth in 4 days.


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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16215070 - 05/11/12 03:06 PM (1 year, 11 months ago)

I think it had more to do with the procedure than the vendor.


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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16215077 - 05/11/12 03:08 PM (1 year, 11 months ago)

Quote:

DrCrumbs said:
I think it had more to do with the procedure than the vendor.





why do you think that? I prepared the jars the exact same way, and with the new spores i got growth in 4 days, compared to 2 weeks from the first batch of spores they sent me.


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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16215128 - 05/11/12 03:17 PM (1 year, 11 months ago)

Its because you were shooting a MS syringe strait on Rye.


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Re: No Growth RYE - PICS [Re: DrCrumbs]
    #16215147 - 05/11/12 03:21 PM (1 year, 11 months ago)

yea, i guess it is hit and miss, but the good thing is they are all colonizing now!


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Re: No Growth RYE - PICS [Re: bootsyyy]
    #16215179 - 05/11/12 03:30 PM (1 year, 11 months ago)

Yeah, very cool. Mycology is so much fun!


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