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 darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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 First post/First grow 
#16157731 - 04/30/12 12:18 AM (1 year, 24 days ago) |
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Air Pump (for humidity control in the terrarium)
10-gallon aquarium
Myco box
   
Jar #1 (4 sides)
   
Jar #2 (4 sides)
  
Jar #3 (3 sides)
   
Jar #4 (4 sides)
 
Jar #5 (2 sides)
Hey all. This is my first post! Not surprisingly this is also my first grow. I decided to keep it simple and go with the PF-Tek, and my species/strain was Psilocybe Cubensis/Golden Teachers. I stuck with the substrate discussed in the Tek, 140ml of vermiculite and 40ml of BRF. I sterilized using a pressure cooker for 90 minutes. My jars were first inoculated, with 1cc per jar, on April 15th.
For the first 5 days I did not see any germination, then I began noticing a tiny little patch of fuzz in a couple of the jars. Then it was just time. As you can see my jars are doing well. Colonization is slower in some than it is in others, and in a few jars there is one injection site that has just finally started to germinate.
- Is there any reason for this late germination?
- What causes colonization rates to differ for jars made in the same environment?
I am guessing that by the looks of how fast the mycelium is spreading that I will see my first jars reach 100% colonization sometime this week. I am going out of town this Thursday (May 3rd) and staying gone until the next Saturday (May 12th).
- Will any jars that are 100% colonized before I leave will be okay to stay in the jars until I return?
-How long can jars be colonized before they NEED to be removed?
I'm new to mycology and am excited everyday that I see new growth. I cannot wait to get back from my trip out of town and have some birthdays! I'll post pics and bi-weekly updates on this grow.
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"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
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mark6587
enthusiast
Registered: 03/14/12
Posts: 58
Loc: south-east USA
Last seen: 1 day, 19 hours
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Quote:
Is there any reason for this late germination
There is, but its probably nothing to do with anything you did. Each spore has to find another spore that its compatible with before it can start producing mycelium. It's kind of like human sex cells except way different haha. It could also be because of variations of moisture and nutrients in the individual jars due to everything not being stirred up quite enough.
Quote:
What causes colonization rates to differ for jars made in the same environment
When you use a multispore syringe there are thousands of substrains floating around in your syringe which means that while all of your jars are Golden Teacher they aren't all the exact same type. This means different colonization rates and fruit sizes etc.
Quote:
Will any jars that are 100% colonized before I leave will be okay to stay in the jars until I return
After jars appear to be 100% colonized you still need to leave them sit for at least a week with a month being optimal. This is called the consolidation period.
Quote:
How long can jars be colonized before they NEED to be removed
I wouldn't wait much longer than a month but you can refrigerate them to extend their shelf-life.
Also welcome to the community and congrats on getting along this far.
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The stories and information posted by myself are artistic works of fiction and falsehood. Only a fool would take anything I have posted here as fact.
Blue oyster prints for trade
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pf madmax
Herbal Enthusiast
Registered: 04/27/12
Posts: 53
Loc: Antartica
Last seen: 9 months, 29 days
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Re: First post/First grow [Re: mark6587]
#16157955 - 04/30/12 01:12 AM (1 year, 24 days ago) |
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I Like your setup, can't wait to see how yours turn out!
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Make friends not enemies. In the end, we all just want to be loved.
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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Re: First post/First grow [Re: mark6587]
#16157977 - 04/30/12 01:20 AM (1 year, 24 days ago) |
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Thanks for the answers, mark. I knew I had to let them sit at least a week, but I didn't know they could sit for a month. That takes a load off my mind, I was worried that the cultures might start fruiting in vitro and cause complications.
Also, I am wondering what kind of light setup I may need for fruiting?
And thanks, pf madmen. I'm excited to see how it turns out to. This is my first run, if this is successful with no complications, next time I'll inoculate more jars. Eventually I hope to start doing bulk grows.
Also, my father is diabetic and is looking for more natural ways to regulate his body's insulin, and I showed him an article I read a few months back on Agaricus campestris. So, after some practice with myco, I want to try to grow a batch.
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"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
Edited by darkliight (04/30/12 01:26 AM)
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mark6587
enthusiast
Registered: 03/14/12
Posts: 58
Loc: south-east USA
Last seen: 1 day, 19 hours
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I use a 6500 kelvin dual fluorescent fixture setup behind my martha. You can get a very basic fixture at home depot for $15 and the bulbs were around $7 each. Essentially You want your light in the blue spectrum which is 6000 K and up (I believe). At stores they are marked as daylight or sunlight bulbs.
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The stories and information posted by myself are artistic works of fiction and falsehood. Only a fool would take anything I have posted here as fact.
Blue oyster prints for trade
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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Re: First post/First grow [Re: mark6587]
#16158006 - 04/30/12 01:29 AM (1 year, 24 days ago) |
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Ok, good. I have a MMJ grow as well, so I have some fluoro's set aside for clones. I'll have to make sure they are the right spectrum, though.
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"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
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mark6587
enthusiast
Registered: 03/14/12
Posts: 58
Loc: south-east USA
Last seen: 1 day, 19 hours
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I'm sure they're fine if you were using them for that haha
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The stories and information posted by myself are artistic works of fiction and falsehood. Only a fool would take anything I have posted here as fact.
Blue oyster prints for trade
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 cubes4cancer
C0nn0IssuR Of s0rtS
Registered: 01/12/12
Posts: 1,527
Loc: thiswashereandthatwaswhen
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Re: First post/First grow [Re: mark6587]
#16158042 - 04/30/12 01:39 AM (1 year, 24 days ago) |
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just a thought here darkliight,
i see you have your supplies in a plastic tub .so why not use your perlite to put together a SGFC(shotgun fruiting chamber) out of that? .
just use the fishtank to make a (PMP)poor mans pod with hydroton once you get the swing of things.
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$$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
  
ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc.
In other words,it's hard to fuck up a cube grow.
RR
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mark6587
enthusiast
Registered: 03/14/12
Posts: 58
Loc: south-east USA
Last seen: 1 day, 19 hours
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Wasn't the sgfc designed to replace the pmp? I have heard several times from several trusted cultivators that the pmp is outdated. Not trying to rustle any jimmies..
--------------------
The stories and information posted by myself are artistic works of fiction and falsehood. Only a fool would take anything I have posted here as fact.
Blue oyster prints for trade
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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Actually, you know, I thought about doing that. Now that you suggested it I think that I might. For now, I am just trying to make the time to go by as fast as possible.
I see lots of MJ  and Comedy Central in my future, after work and school of course  .
Edited by darkliight (04/30/12 01:55 AM)
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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May 1st Update:
All 5 Jars are looking very nice. Mycelium growth is still fast and consistent, with no observable slowing in growth. No contaminations spotted in any of the jars.
In Jars #1 and #2, the least colonized inoculation points are showing increased mycelium growth and look like they will continue to spread without hesitation
Jar #5 should be ready to begin its consolidation period. And I will be keeping them in consolidation for about a week and a half to two weeks.
I will update again Thursday (with pics!) before I leave to go out of town. Then fresh new pics and updates when I get back.
---------------------------------------------------------------------------
So does anyone around here have any experience with buying compost for mycelium from online vendors?
- Is it safe/reputable?
- Pasteurized or non-pasteurized?
I am not looking to jump into a large grow op using bulk substrate, I am just gathering materials for future experiments.
Also, I have decided that after my trip i will be turning my Myco box into a SGFC.
Also, before fruiting begins (I don't want to be asking questions last minute). I have to work weekdays and was wondering if my air pump, an air stone, and some water+ hydrogen peroxide would be good for while I was at work. I can mist and spray all day after I get home.
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"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
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pf madmax
Herbal Enthusiast
Registered: 04/27/12
Posts: 53
Loc: Antartica
Last seen: 9 months, 29 days
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I noticed with my mycelium growth that it seemed to slow and then expand through out the cake quickly like it went inside the cake then popped back to the surface. Not sure if that's what really happens thought!
PS i made my FC into a Shotgun with a soldering iron turning it on/off so it was just hot enough to melt but not burn the plastic, makes adding the holes quick! And the increase in fresh air exchange has already boosted growth a lot in the first 16 hours.
check out my first grow
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Make friends not enemies. In the end, we all just want to be loved.
Edited by pf madmax (05/05/12 02:34 AM)
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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Hello all!
Back in town from vacation and it was great. Spent a week in SW Florida with the GF and took mom so she could have a week long Mother's Day present. I know, I know, I'm a good son lol.
Anyway, I returned excited and eager to see my cakes an inspect them for further growth. Jars 1, 3, 4, and 5 are doing great. There was some halted mycelium growth in Jars 1 and 4, probably due to the tape still on the holes. I removed the tape from Jars 1, 3, 4, and 5 in hopes that it will expedite mycelium growth.
As someone of you may have noticed, I neglected to add any details about Jar 2, this was not an accident. I am worried about some strange growth I have noticed about 3cm to the left of one of the inoculation points. It is grayish blue-green to me, but I had someone else look at it and they said they seen nothing. I wanted to know what you guys think.
- Will removing the tape from the inoculation holes expedite mycelium growth or will it cause the cakes to lose moisture?
- Is the spot marked in the picture, the starting of Trichoderma? It looks a lot like it to me, but I want to be sure.
- In the event that Jar 2 is contaminated, any disposal ideas? Could I recycle the jar? Or is it just easier to completely dispose of it?
And now what you've all been waiting for!!!!
::::PICTURES::::
Jar 1
Jar 2
Jar 2 (Better view of the possible contam)
Jar 3
Jar 4
Jar 5
--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
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mrmojoshroomin
All-summa BeenNoddin
Registered: 01/25/12
Posts: 611
Loc: OH
Last seen: 10 months, 21 days
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Doesnt look like contam to me, just a little bit wet from condensation, wetter=slower colonization,keep an eye on it though, all in all, looking good
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herfy
Stranger
Registered: 01/26/12
Posts: 111
Last seen: 10 months, 14 days
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Take the ELECTRICAL tape off all you're lids they need to breath..the verm acts as a barrier for contams.
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herfy
Stranger
Registered: 01/26/12
Posts: 111
Last seen: 10 months, 14 days
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Re: First post/First grow [Re: herfy]
#16222516 - 05/13/12 02:22 AM (1 year, 11 days ago) |
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Jar number 2 needs to be disposed of,what i do is take it outside away from the house and open it up and dump it out..The jar can be reused just boil it in water before using it again..
but I am a newb also.so i could be wrong..
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pf madmax
Herbal Enthusiast
Registered: 04/27/12
Posts: 53
Loc: Antartica
Last seen: 9 months, 29 days
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Re: First post/First grow [Re: herfy]
#16223435 - 05/13/12 11:09 AM (1 year, 11 days ago) |
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Black/green is contam, yellowish/egg yolk/blue discoloration is just mycelium piss and blue is just bruising that can happen from dropping a jar.
Jar 2 def looks contaminated though. Toss that shit! You can try to stick it in a pile of manure outside and it may colonize the pile if your lucky =]
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Make friends not enemies. In the end, we all just want to be loved.
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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Re: First post/First grow [Re: herfy]
#16226814 - 05/14/12 12:19 AM (1 year, 10 days ago) |
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Quote:
herfy said:
Take the ELECTRICAL tape off all you're lids they need to breath..the verm acts as a barrier for contams.
I did take the tape off of all of the Jars. Except Jar 2, due to its contam.
May 14th Update
When I went to dispose of the contaminated cake (Jar 2), I opened the jar to find that the top dry layer of verm had been disturbed any mycelium both good and bad had begun to colonize it. I emptied the cake out, outside and broke it open at the discoloration and found much more of the blue-green fungus (Tricho). I wish I would have taken pictures, although it was bad for the outcome of my grow. It was very interesting to look at the contamination, it smelled much more foul than the mushroomy mycelium.
After examining and disposing of the cake, I washed up and changed clothes before I went back to my grow area. I put the clothes in the wash so that they could be cleaned of any spores I may have picked up during the disposal.
Jars 1, 3, 4, and 5 are doing much better. Mycelium growth has sped up a bit, I will take pictures on my Tuesday report.
Also, I will try my best to do updates every other day, and posts with pictures every other post.
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"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
Edited by darkliight (05/14/12 12:29 AM)
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darkliight
Aborted
Registered: 04/29/12
Posts: 178
Last seen: 3 days, 19 hours
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May 16th Update
Mycelium growth is continuing in Jars 1 and 2, while Jars 3 and 5 are 100% colonized. I will keep Jars 3 and 5 in consolidation until Jars 1 and 2 are 100% colonized for one week. So I am estimating a birthday around the end of the month. Not much else to report other than a strange spot on Jar 2. Probably nothing other than uncolonized substrate but just wanted some extra opinions. Now for the pics!
Jar 1 (Only a couple days until 100% I'm betting)
Jar 2 (Colonizing slower than the others even though it was one of the first to be inoculated)
Jar 2 (The strange spot I was talking about)
Jar 2c (A more complete view of the uncolonized substrate)
Jar 3 (100% colonized)
Jar 5 (100% colonized)
Next update is Thursday! I may post pics, depends on if anything interesting pops up. Maybe some primordia 
--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson
New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org
First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE
Everything I say on this site is a lie.
Let the paradox begin.
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Wallflower
Registered: 05/10/12
Posts: 721
Last seen: 8 months, 27 days
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Quote:
darkliight said:Also, my father is diabetic and is looking for more natural ways to regulate his body's insulin, and I showed him an article I read a few months back on Agaricus campestris. So, after some practice with myco, I want to try to grow a batch.
Heya, I was just browsing and when I read this it reminded me of an experiment a coworker of mine did a couple years ago.
Apparently cinnamon (true cinnamon, not the stuff in most grocery stores that comes from like a bastard cousin of the true plant) can cause increases in insulin production/release. This makes it actually a little hazardous for people with hypoglycemia, but could be a great natural help for diabetics. True cinnamon can be expensive and usually has to be bought online, and again most cinnamon in grocery stores is not true cinnamon. But anyway, my coworker reported that adding true cinnamon to her coffee seemed to help. Not sure if this was placebo or genuine, but there are a lot of articles out there about genuine science behind this. So just wanted to mention in case he hasn't tried it yet!
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