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 psychonautic
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 Unusual Green Color at Base? 
#15946651 - 03/14/12 04:55 PM (1 year, 2 months ago) |
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SWIM has some Pint-sized PF TEK cakes in a terrarium, and instead of doing what they're supposed to, they do this:
The two cakes have been in the chamber for 4 full days. Speaking with someone else, he learned that they puffed up due to lack of air-exchange, and now I'm seeing green-mold. He tried rubbing a dry q-tip, and it actually comes off of the substrate material. Though, he's afraid of removing it for spreading more.
Best case-scenario?
Edited by psychonautic (03/14/12 04:58 PM)
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 TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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best case scenario, you don't get any more contams. But you have to get rid of that cake.
Ditch the cake, wipe down the FC w/ some warm soapy water.
the Q-tip test is complete BS. Once it goes green, it's done & best outside away from any future grows.
--------------------
Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
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"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
   How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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psychonautic
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So, the second cake is okay though?
Edit:
What's the best way to dispose of this thing?
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.:PsillyPsycho:.
Edited by psychonautic (03/14/12 05:17 PM)
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TranscendingLife
I Don't Need a Life to Live
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second one looks fine.
throw it in your garden.
--------------------
Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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fungusapien
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Well, it seems that your contam has already sporulated, and you have other cakes in such close proximity, so it's quite possible it will show up on some of those other cakes. I'd take a ziplock or some kind of plastic bag and just grab it with that and close it up trying not to move it much, then take it outside somewhere, dump it and bury it.
And like TL said, you should clean out your FC.
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"It achieved symbiosis with human society early by associating itself with domesticated cattle." - T. McKenna
leave yourself behind
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punkrocker292004
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Re: Unusual Green Color at Base? [Re: fungusapien]
#15946980 - 03/14/12 06:17 PM (1 year, 2 months ago) |
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get it out now 
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EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
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billy
Registered: 07/13/11
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cake is toast. clean your fc walls and rinse the pearlite. keep a very close eye on the other cakes. are they sitting on a bed of verm in the jar lid? if so you might want to get rid of the extra. its uncolonized and stays moist. just what contams like that need.....
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Rest NVS
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Re: Unusual Green Color at Base? [Re: billy]
#15947109 - 03/14/12 06:50 PM (1 year, 2 months ago) |
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Get rid of them (bury in garden) and clean your FC. Once you see the green, its not looking good, and that first cake is out of control! I am pretty new at this so my advice might not be worth much, but Id say that its better to be safe then sorry, or you have the chance of having that problem keep being a problem. Take care of it now!
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Javadog
Continuing along
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Yeah, you are learning. ...that cake should have been identified as
infected earlier, but there is little else for you to do but what has
been suggested.
Good luck,
JD
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Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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psychonautic
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Re: Unusual Green Color at Base? [Re: Javadog]
#15947193 - 03/14/12 07:23 PM (1 year, 2 months ago) |
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Yup, this is SWIM's first attempt, he has 3 other pint jars that have stalled at like 98% colonization. Hopefully they don't stop growing with such little left.
Either way, SWIM is confident about the other cake. It's pinning already, and seems unphased by the whole thing.
Any suggestions on what SWIM could do to help the jars finish colonizing? It's been 8 weeks.... 
Does one suggest the cakes sit on aluminum foil vs. moist verm?
EDIT:
Did a total removal of the perlite, soapy anti-bacterial scrub inside, fresh-moist perlite in as well.
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.:PsillyPsycho:.
Edited by psychonautic (03/14/12 07:25 PM)
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Rest NVS
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I had the same problem with some of my jars and I just turned the heat up on the incubation chamber to 88 and boom!!! they started growing again. Thats my best suggestion! It would be a shame to toss jars. Mycelia grows faster at a higher temp! Hope it helps
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psychonautic
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15947275 - 03/14/12 07:38 PM (1 year, 2 months ago) |
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Quote:
Rest NVS said:
I had the same problem with some of my jars and I just turned the heat up on the incubation chamber to 88 and boom!!! they started growing again. Thats my best suggestion! It would be a shame to toss jars. Mycelia grows faster at a higher temp! Hope it helps:D
It does actually. SWIM also heard that if you flipped them upside down and smacked the cake off the bottom of the jar it would force air through the holes and make it breathe. So, SWIM left them upside-down for a day, then flipped them back over. It kicked two into gear, but the third one is still like a good solid week of colonization. It's lame because it's been so long thus far.
This is the new situation for the remaining cake:
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.:PsillyPsycho:.
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Rest NVS
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Yep, I have done the same thing and it has helped! The heat seemed to work the best for me. I try to keep them at around 81-82, but sometimes you just need to resort to drastic measures.  LOL
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Rest NVS
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15947324 - 03/14/12 07:47 PM (1 year, 2 months ago) |
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Get some more jars going!!!!! It sucks waiting, but you might as well start some more while these are stalled! You can never have to much LOL Plus once the jars you have now are done fruiting you'll have some more to throw in!
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psychonautic
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15947338 - 03/14/12 07:50 PM (1 year, 2 months ago) |
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Well, I'll say that SWIM has had enormous amounts of fun thus far. The research, the learning.. it's all super effective. Pluss, the pay-off at the end makes everything completely worth it. Even if 100% of it doesn't go right, SWIM will be okay with everything either way. It's been fun thus far, and he's sure it will continue.
SWIM has 1cc left in a syringe, and needs to order more. Though his brother is the one who has to receive them. It's kind of drastic because SWIM can't have spores delivered to his location. Though it's not too difficult.
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.:PsillyPsycho:.
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Rest NVS
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I am having a great time with this!!!!! I am a vet when it comes to growing green, but this has become my new little obsession! My girl hasn't been to happy with me since I seem to have more interest in shrooms, but what can ya do???? LOL Best of luck to you man! keep us informed on the progress!
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Swwert
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15947702 - 03/14/12 08:58 PM (1 year, 2 months ago) |
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Whole Brown Rice Cakes TEK
  
"The mushrooms are just loving you back."
"Maria Sabina is a symbol of wisdom and love."
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pqueue
Vowel Enthusiast
Registered: 08/12/11
Posts: 95
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Quote:
psychonautic said:
Yup, this is SWIM's first attempt, he has 3 other pint jars that have stalled at like 98% colonization. Hopefully they don't stop growing with such little left.
Are you sure it isn't the top verm layer falling down between the cake and the jar? If there has been no change for over a week I'd go ahead and birth 'em. Either they're contaminated or they're ready if they've been sitting a while with no progress.
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pqueue
Vowel Enthusiast
Registered: 08/12/11
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15947961 - 03/14/12 09:38 PM (1 year, 2 months ago) |
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Quote:
Rest NVS said:
I had the same problem with some of my jars and I just turned the heat up on the incubation chamber to 88 and boom!!! they started growing again. Thats my best suggestion! It would be a shame to toss jars. Mycelia grows faster at a higher temp! Hope it helps:D
Please don't give advice if you don't know what you're talking about. 88 is way too hot to incubate your jars. Unless you keep your house below 68 or so you don't need an incubator in the first place. Cubes will grow fine at even lower temps, just more slowly.
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psychonautic
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Re: Unusual Green Color at Base? [Re: pqueue]
#15947962 - 03/14/12 09:38 PM (1 year, 2 months ago) |
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How does the top dry verm falling down between the jar and the cake affect the colonization?
SWIM can get some pictures for you. Just give him a few.
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.:PsillyPsycho:.
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pqueue
Vowel Enthusiast
Registered: 08/12/11
Posts: 95
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Quote:
psychonautic said:
How does the top dry verm falling down between the jar and the cake affect the colonization?
SWIM can get some pictures for you. Just give him a few.
Myc won't colonize dry verm so it'll just sit there between the cake and the jar, especially if the cake has shrunk and the verm is stuck to the jar. How long have they been at 98%? I just reread your other post, if it has been 8 weeks they're definitely either ready or contaminated.
Edited by pqueue (03/14/12 09:42 PM)
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psychonautic
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These are SWIMS 3 other jars. The black lines are expo marker around the outside of the jar to track progress. They've been this close for over a week now, and my other onecake has been in the FC for 4 days. I waited roughly a week before fruiting (saw visible hyphal knots). It's only been since SWIM turned the cakes over and left them for 24 hours that they've really started moving. Though one of the cakes hasn't picked back up since the flip.
SWIM has seen pictures of cakes that aren't 100% covered in mycelium, and he's often wondered what the danger is of birthing a cake before 100% colonization?
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.:PsillyPsycho:.
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pqueue
Vowel Enthusiast
Registered: 08/12/11
Posts: 95
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Yeah definitely not dry verm, those aren't ready yet. How long ago did you inoculate?
You want the substrate to be fully colonized so you don't give contams a place to grow. Once it is colonized the myc is fairly resistant to contams.
Edited by pqueue (03/14/12 09:48 PM)
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pqueue
Vowel Enthusiast
Registered: 08/12/11
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Oh and drop the SWIM crap, we all know you're growing the shrooms 
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psychonautic
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Re: Unusual Green Color at Base? [Re: pqueue]
#15948067 - 03/14/12 09:51 PM (1 year, 2 months ago) |
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SWIM inoculated on Jan 19.
Though, SWIM may have used too little spores during the injection. 1cc in a pint..may not get but one or two flushes, but SWIM figured he could at least get it to colonize. 
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.:PsillyPsycho:.
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pqueue
Vowel Enthusiast
Registered: 08/12/11
Posts: 95
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Quote:
psychonautic said:
SWIM inoculated on Jan 19.
Though, SWIM may have used too little spores during the injection. 1cc in a pint..may not get but one or two flushes, but SWIM figured he could at least get it to colonize.
Ah, I didn't notice you were using pint jars the first time I looked those pics. Try half pints next time, pint jars have a tendency to do exactly what you're seeing. 1cc is fine for brf cakes, you probably could have used a little less without issue. I would go ahead and buy some half pints and get those started. As long as you don't see contams let the pints finish up, no harm at this point, just don't reuse them for brf cakes. Two months is definitely a long time though.
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psychonautic
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Re: Unusual Green Color at Base? [Re: pqueue]
#15948122 - 03/14/12 09:59 PM (1 year, 2 months ago) |
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Quote:
pqueue said:
Ah, I didn't notice you were using pint jars the first time I looked those pics. Try half pints next time, pint jars have a tendency to do exactly what you're seeing. 1cc is fine for brf cakes, you probably could have used a little less without issue. I would go ahead and buy some half pints and get those started. As long as you don't see contams let the pints finish up, no harm at this point, just don't reuse them for brf cakes. Two months is definitely a long time though.
The issue with the whole thing was SWIM couldn't find half-pints without using the internet, and he didn't want to wait. Though he technically could've just waited anyway because he didn't get started for a long time. He figures that's the next adjustment, and maybe a lid with a CPU fan for air-flow.
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.:PsillyPsycho:.
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pqueue
Vowel Enthusiast
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Quote:
The issue with the whole thing was SWIM couldn't find half-pints without using the internet, and he didn't want to wait. Though he technically could've just waited anyway because he didn't get started for a long time. He figures that's the next adjustment, and maybe a lid with a CPU fan for air-flow.
Patience is the number one requirement for this hobby. You won't get far without it.
I can't see your fruiting chamber in any of the pics but a fan won't help. Since you're using perlite I'll assume you built a SGFC. If you don't know what a SGFC is then this is the perfect time to get acquainted with the search function 
Remember: every 2 inches, and all SIX sides! Follow the tek to the T and you'll get what you're after.
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psychonautic
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Re: Unusual Green Color at Base? [Re: pqueue]
#15948290 - 03/14/12 10:31 PM (1 year, 2 months ago) |
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SWIM has removed the divider, as the humidity is solid at 99%, plus he has a 30gallon air-pump to increase humidity/temperature. SWIM learned he needed to fan his FC a lot more often than initially expected, though that's okay because he doesn't mind tending to his sheep.
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.:PsillyPsycho:.
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punkrocker292004
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i think your hygrometer is busted i see 0 condensation on the walls of that tank
i think you should consider making a shotgun fc
--------------------
EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
watch me
MHRB
got attitude?
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Rest NVS
Seeker of knowledge
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Re: Unusual Green Color at Base? [Re: pqueue]
#15948470 - 03/14/12 11:07 PM (1 year, 2 months ago) |
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Quote:
pqueue said:
Quote:
Rest NVS said:
I had the same problem with some of my jars and I just turned the heat up on the incubation chamber to 88 and boom!!! they started growing again. Thats my best suggestion! It would be a shame to toss jars. Mycelia grows faster at a higher temp! Hope it helps:D
Please don't give advice if you don't know what you're talking about. 88 is way too hot to incubate your jars. Unless you keep your house below 68 or so you don't need an incubator in the first place. Cubes will grow fine at even lower temps, just more slowly.
The only reason I had them at that temp was to start a stalled growth, and gosh it worked! The cakes finished colonizing and fruited nicely. I normally have it at 81-82. And I was just giving a suggestion, calm down. This post started from someone asking for advice and things that worked for other people. Isn't that the point of a fourm? I agree 88 is pretty warm, but far from hurting the cakes. Yes, room temp will work if you want to take the slow route, but they will colonize much faster in a warmer environment! Anyone can take my advice for what it is because i am certainly not a pro, but it worked for me. To each their own I guess.
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Rest NVS
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Quote:
punkrocker292004 said:
i think your hygrometer is busted i see 0 condensation on the walls of that tank
I agree! 
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psychonautic
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Quote:
punkrocker292004 said:
i think your hygrometer is busted i see 0 condensation on the walls of that tank
i think you should consider making a shotgun fc
Those pictures are before SWIM actually ran the first test. It works beautifully. Plus, SWIM has a heating pad below the tank that will only run in 1 hour bursts. SWIM's major difficulty at this point is air. Lots and lots of fresh air. His cakes started "foaming" because they didn't have enough air.
No, the hygrometer is 1 of 2, and both work splendidly. SWIM interchanges the two so as to not break the electronics on board with prolonged exposure to high RH.
What do you suggest as a good hygrometer other than an electronic one?
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.:PsillyPsycho:.
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 4,399
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15948537 - 03/14/12 11:20 PM (1 year, 2 months ago) |
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Quote:
Rest NVS said:
Quote:
pqueue said:
Quote:
Rest NVS said:
I had the same problem with some of my jars and I just turned the heat up on the incubation chamber to 88 and boom!!! they started growing again. Thats my best suggestion! It would be a shame to toss jars. Mycelia grows faster at a higher temp! Hope it helps:D
Please don't give advice if you don't know what you're talking about. 88 is way too hot to incubate your jars. Unless you keep your house below 68 or so you don't need an incubator in the first place. Cubes will grow fine at even lower temps, just more slowly.
The only reason I had them at that temp was to start a stalled growth, and gosh it worked! The cakes finished colonizing and fruited nicely. I normally have it at 81-82. And I was just giving a suggestion, calm down. This post started from someone asking for advice and things that worked for other people. Isn't that the point of a fourm? I agree 88 is pretty warm, but far from hurting the cakes. Yes, room temp will work if you want to take the slow route, but they will colonize much faster in a warmer environment! Anyone can take my advice for what it is because i am certainly not a pro, but it worked for me. To each their own I guess.
Yeah but when get into temperatures like that it actually slows the growth so your cakes would have finished earlier if left at 81-82F which is still unnecessarily high.
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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OK...first off, stop w/ the "SWIM" bullshit or the "AFOAF."
You're growing, we all know it, no need to shit a shitter.
Build a proper SGFC & use the proper jars.
You'll have around 95% success w/ using MS syringes for it.
www.mushroomvidoes.com has the entire PF tek for free on it.
Learn it, love it, live it.
those temperatures are perfect for mold & bacterial growth.
Keep them at room temp (68-76F) w/ indirect 12/12 lighting during colonization.
--------------------
Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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Javadog
Continuing along
Registered: 05/03/10
Posts: 7,286
Loc: USA
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Quote:
TranscendingLife said:
OK...first off, stop w/ the "SWIM" bullshit or the "AFOAF."
You're growing, we all know it, no need to shit a shitter.
Build a proper SGFC & use the proper jars.
You'll have around 95% success w/ using MS syringes for it.
www.mushroomvidoes.com has the entire PF tek for free on it.
Learn it, love it, live it.
those temperatures are perfect for mold & bacterial growth.
Keep them at room temp (68-76F) w/ indirect 12/12 lighting during colonization.
The perfect response, as usual, TL!
--------------------
Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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Rest NVS
Seeker of knowledge
Registered: 03/05/12
Posts: 88
Loc: Colorado
Last seen: 8 months, 29 days
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Quote:
TranscendingLife said:
OK...first off, stop w/ the "SWIM" bullshit or the "AFOAF."
You're growing, we all know it, no need to shit a shitter.
Build a proper SGFC & use the proper jars.
You'll have around 95% success w/ using MS syringes for it.
www.mushroomvidoes.com has the entire PF tek for free on it.
Learn it, love it, live it.
those temperatures are perfect for mold & bacterial growth.
Keep them at room temp (68-76F) w/ indirect 12/12 lighting during colonization.
YEP
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fungusapien
Nomad
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Posts: 317
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Re: Unusual Green Color at Base? [Re: Javadog]
#15948735 - 03/15/12 12:04 AM (1 year, 2 months ago) |
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--------------------
"It achieved symbiosis with human society early by associating itself with domesticated cattle." - T. McKenna
leave yourself behind
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Kizzle
Misanthrope
Registered: 08/30/11
Posts: 4,399
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Re: Unusual Green Color at Base? [Re: fungusapien]
#15951231 - 03/15/12 04:29 PM (1 year, 2 months ago) |
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Quote:
TranscendingLife said:
OK...first off, stop w/ the "SWIM" bullshit or the "AFOAF."
You're growing, we all know it, no need to shit a shitter.
But what if his name really is Swim?
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Rest NVS
Seeker of knowledge
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Posts: 88
Loc: Colorado
Last seen: 8 months, 29 days
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Re: Unusual Green Color at Base? [Re: Kizzle]
#15951255 - 03/15/12 04:34 PM (1 year, 2 months ago) |
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Quote:
Kizzle said:
Quote:
TranscendingLife said:
But what if his name really is Swim?
 That was kinda funny!
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Javadog
Continuing along
Registered: 05/03/10
Posts: 7,286
Loc: USA
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Re: Unusual Green Color at Base? [Re: Rest NVS]
#15951385 - 03/15/12 04:59 PM (1 year, 2 months ago) |
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20 people named "Richard Swim" in the US alone:
http://names.whitepages.com/richard/swim
--------------------
Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,403
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Re: Unusual Green Color at Base? [Re: Kizzle]
#15951439 - 03/15/12 05:10 PM (1 year, 2 months ago) |
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Quote:
Kizzle said:
Quote:
TranscendingLife said:
OK...first off, stop w/ the "SWIM" bullshit or the "AFOAF."
You're growing, we all know it, no need to shit a shitter.
But what if his name really is Swim?
 then he shouldn't be using his real name...
--------------------
Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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fungusapien
Nomad
Registered: 02/09/09
Posts: 317
Loc: PNW
Last seen: 4 months, 22 days
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"It achieved symbiosis with human society early by associating itself with domesticated cattle." - T. McKenna
leave yourself behind
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punkrocker292004
i am a liar
Registered: 12/17/09
Posts: 2,819
Loc:
Last seen: 12 days, 7 hours
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Re: Unusual Green Color at Base? [Re: fungusapien]
#15951650 - 03/15/12 06:06 PM (1 year, 2 months ago) |
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EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
watch me
MHRB
got attitude?
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OOOG
Stranger
Registered: 03/15/12
Posts: 57
Last seen: 1 year, 2 months
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